Maternal plasma extracellular vesicles tsRNA as potential biomarkers for assessing preterm labor risk DOI Creative Commons

Xinrui Sun,

Ying Feng,

Liang Xie

и другие.

BMC Pregnancy and Childbirth, Год журнала: 2025, Номер 25(1)

Опубликована: Май 10, 2025

Spontaneous preterm labor (PTL) accounts for approximately 70% of births, posing significant risks to both maternal and neonatal health. Current predictive biomarkers lack sufficient reliability, underscoring the need non-invasive dependable indicators. Emerging research indicates that tRNA-derived small RNAs (tsRNAs) are involved in various diseases; however, their potential association with PTL remains underexplored. Bioinformatics analyses public GEO datasets (PRJNA415953 PRJNA428989) were conducted identify tsRNAs associated PTL. Validation was performed using plasma extracellular vesicles samples collected at 12 weeks gestation from patients (n = 45) healthy controls 38). Functional assays used assess impact tsRNA1 (tRNA-Gly-GCC-5p-tRF-921) on extravillous trophoblast (EVT) function, including apoptosis, migration, invasion, endothelial-like tube formation HTR8/SVneo cells. Transcriptomic sequencing tsRNA1-mediated pathways, DNA methylation patterns predicted based transcriptomic data. Statistical significance determined Student's t-test. Two tsRNAs, tsRNA3 (tRNA-Gly-GCC-5p-tR-half-368), significantly upregulated patient compared controls. Overexpression impaired EVT increased altered profiles, implicating its critical role mechanisms. This study identifies as a key regulator dysfunction placental pathology The findings provide novel insights into mechanistic highlight promising biomarker early risk stratification prediction condition. Not applicable.

Язык: Английский

tRNA-derived small RNAs: their role in the mechanisms, biomarkers, and therapeutic strategies of colorectal cancer DOI Creative Commons
Bo Zhang, Yi Pan, Zhe Li

и другие.

Journal of Translational Medicine, Год журнала: 2025, Номер 23(1)

Опубликована: Янв. 13, 2025

Colorectal cancer (CRC) is the third most prevalent malignancy and second leading cause of cancer-related mortality worldwide, with an increasing shift towards younger age onset. In recent years, there has been recognition significance tRNA-derived small RNAs (tsRNAs), encompassing fragments (tRFs) tRNA halves (tiRNAs). Their involvement in regulating translation, gene expression, reverse transcription, epigenetics gradually come to light. Emerging research revealed dysregulation tsRNAs CRC, implicating their role CRC initiation progression, highlighting potential early diagnosis, prognosis, therapeutic strategies. Although clinical application still its stages, findings highlight a close relationship between biogenesis function tsRNAs, chemical modifications, tumor immune microenvironment (TIME). Additionally, similar other RNAs, can be effectively delivered via nanoparticles (NPs). Consequently, future should focus on elucidating concerning base TIME regulation, immunotherapy, NPs delivery systems facilitate translation.

Язык: Английский

Процитировано

1
tRF-5028c disrupts trophoblast function in recurrent spontaneous abortion by inhibiting CRKL-mediated Rap1 signaling pathway DOI Creative Commons
Jialyu Huang, Jiawei Wang, Shuang Wang

и другие.

Cellular & Molecular Biology Letters, Год журнала: 2025, Номер 30(1)

Опубликована: Март 5, 2025

Recurrent spontaneous abortion (RSA) affects approximately 1-5% of childbearing women and poses a significant threat to global reproductive health. Transfer RNA-derived small RNAs (tsRNAs) are novel class noncoding implicated in various human diseases. However, the role mechanism tsRNAs regulating trophoblast function during RSA development remain unknown. High-throughput sequencing was performed analyze differential villous tissues patients with controls. CCK-8, transwell assay, flow cytometry were detect effects tRF-5028c on proliferation, migration, invasion, apoptosis extravillous cell line HTR-8/SVneo. The target genes predicted via bioinformatic analysis verified by dual luciferase reporter gene assay. Moreover, pregnant mice injected mimics confirm findings vivo. A total 1907 detected, which 298 differentially expressed tissues. significantly upregulated group compared control. Functionally, overexpression inhibited HTR-8/SVneo invasion promoted apoptosis, whereas knockdown showed opposite effects. Mechanically, suppressed CRKL expression directly binding its 3'-untranslated region, thus inactivating downstream C3G/Rap1 signaling pathway. Finally, injection increased embryo absorption rate mice. upregulation impaired facilitate targeting CRKL-mediated Rap1 provide first evidence tsRNA dysregulation pathogenesis lay foundation for potential targeted therapies.

Язык: Английский

Процитировано

1
Methylation modifications in tRNA and associated disorders: Current research and potential therapeutic targets DOI Creative Commons
Zhijing Wu, Ruixin Zhou,

Baizao Li

и другие.

Cell Proliferation, Год журнала: 2024, Номер 57(9)

Опубликована: Июнь 28, 2024

High-throughput sequencing has sparked increased research interest in RNA modifications, particularly tRNA methylation, and its connection to various diseases. However, the precise mechanisms underpinning development of these diseases remain largely elusive. This review sheds light on roles several methylations (m1A, m3C, m5C, m1G, m2G, m7G, m5U, Nm) diverse biological functions, including metabolic processing, stability, protein interactions, mitochondrial activities. It further outlines linked aberrant related enzymes, potential underlying mechanisms. Moreover, disruptions regulation abnormalities tRNA-derived small RNAs (tsRNAs) contribute disease pathogenesis, highlighting their as biomarkers for diagnosis. The also delves into exploration drugs targeting methylation emphasizing therapeutic prospects modulating processes. Continued is imperative a comprehensive comprehension integration molecular diagnosis treatment.

Язык: Английский

Процитировано

5
The Mechanisms, Research Status, and Future Prospects of m6A Modification in Breast Cancer DOI Creative Commons

Xiu Xue‐mei,

Chen Yang,

Ju Wen‐ting

и другие.

The Journal of Gene Medicine, Год журнала: 2025, Номер 27(2)

Опубликована: Фев. 1, 2025

ABSTRACT N6‐methyladenosine (m6A) modification is a significant methylation alteration frequently observed in eukaryotic RNAs, garnering considerable attention the field of breast cancer research recent years. The m6A profoundly influences onset, progression, and prognosis by regulating RNA stability, translation efficiency, degradation processes. Numerous studies have demonstrated that regulatory factors, including METTL3, METTL14, ALKBH5, play pivotal roles cells, affecting cell proliferation, metastasis, drug resistance. Furthermore, interactions between non‐coding as well its role tumor microenvironment, increasingly attracted researchers' interest. Although numerous elucidated dual cancer, specific molecular mechanisms remain to be thoroughly investigated. Future should explore various aspects, different subtypes involvement chemotherapy resistance, with microenvironment. This exploration will contribute advancements diagnosis treatment cancer. present article aims systematically summarize progress on offering novel insights strategies for future related clinical applications.

Язык: Английский

Процитировано

0
Maternal plasma extracellular vesicles tsRNA as potential biomarkers for assessing preterm labor risk DOI Creative Commons

Xinrui Sun,

Ying Feng,

Liang Xie

и другие.

BMC Pregnancy and Childbirth, Год журнала: 2025, Номер 25(1)

Опубликована: Май 10, 2025

Spontaneous preterm labor (PTL) accounts for approximately 70% of births, posing significant risks to both maternal and neonatal health. Current predictive biomarkers lack sufficient reliability, underscoring the need non-invasive dependable indicators. Emerging research indicates that tRNA-derived small RNAs (tsRNAs) are involved in various diseases; however, their potential association with PTL remains underexplored. Bioinformatics analyses public GEO datasets (PRJNA415953 PRJNA428989) were conducted identify tsRNAs associated PTL. Validation was performed using plasma extracellular vesicles samples collected at 12 weeks gestation from patients (n = 45) healthy controls 38). Functional assays used assess impact tsRNA1 (tRNA-Gly-GCC-5p-tRF-921) on extravillous trophoblast (EVT) function, including apoptosis, migration, invasion, endothelial-like tube formation HTR8/SVneo cells. Transcriptomic sequencing tsRNA1-mediated pathways, DNA methylation patterns predicted based transcriptomic data. Statistical significance determined Student's t-test. Two tsRNAs, tsRNA3 (tRNA-Gly-GCC-5p-tR-half-368), significantly upregulated patient compared controls. Overexpression impaired EVT increased altered profiles, implicating its critical role mechanisms. This study identifies as a key regulator dysfunction placental pathology The findings provide novel insights into mechanistic highlight promising biomarker early risk stratification prediction condition. Not applicable.

Язык: Английский

Процитировано

0