The Mechanisms, Research Status, and Future Prospects of m6A Modification in Breast Cancer DOI Creative Commons

Xiu Xue‐mei,

Chen Yang,

Ju Wen‐ting

et al.

The Journal of Gene Medicine, Journal Year: 2025, Volume and Issue: 27(2)

Published: Feb. 1, 2025

ABSTRACT N6‐methyladenosine (m6A) modification is a significant methylation alteration frequently observed in eukaryotic RNAs, garnering considerable attention the field of breast cancer research recent years. The m6A profoundly influences onset, progression, and prognosis by regulating RNA stability, translation efficiency, degradation processes. Numerous studies have demonstrated that regulatory factors, including METTL3, METTL14, ALKBH5, play pivotal roles cells, affecting cell proliferation, metastasis, drug resistance. Furthermore, interactions between non‐coding as well its role tumor microenvironment, increasingly attracted researchers' interest. Although numerous elucidated dual cancer, specific molecular mechanisms remain to be thoroughly investigated. Future should explore various aspects, different subtypes involvement chemotherapy resistance, with microenvironment. This exploration will contribute advancements diagnosis treatment cancer. present article aims systematically summarize progress on offering novel insights strategies for future related clinical applications.

Language: Английский

tRNA-derived small RNAs: their role in the mechanisms, biomarkers, and therapeutic strategies of colorectal cancer DOI Creative Commons
Bo Zhang, Yi Pan, Zhe Li

et al.

Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: Jan. 13, 2025

Colorectal cancer (CRC) is the third most prevalent malignancy and second leading cause of cancer-related mortality worldwide, with an increasing shift towards younger age onset. In recent years, there has been recognition significance tRNA-derived small RNAs (tsRNAs), encompassing fragments (tRFs) tRNA halves (tiRNAs). Their involvement in regulating translation, gene expression, reverse transcription, epigenetics gradually come to light. Emerging research revealed dysregulation tsRNAs CRC, implicating their role CRC initiation progression, highlighting potential early diagnosis, prognosis, therapeutic strategies. Although clinical application still its stages, findings highlight a close relationship between biogenesis function tsRNAs, chemical modifications, tumor immune microenvironment (TIME). Additionally, similar other RNAs, can be effectively delivered via nanoparticles (NPs). Consequently, future should focus on elucidating concerning base TIME regulation, immunotherapy, NPs delivery systems facilitate translation.

Language: Английский

Citations

1
tRF-5028c disrupts trophoblast function in recurrent spontaneous abortion by inhibiting CRKL-mediated Rap1 signaling pathway DOI Creative Commons
Jialyu Huang, Jiawei Wang, Shuang Wang

et al.

Cellular & Molecular Biology Letters, Journal Year: 2025, Volume and Issue: 30(1)

Published: March 5, 2025

Recurrent spontaneous abortion (RSA) affects approximately 1-5% of childbearing women and poses a significant threat to global reproductive health. Transfer RNA-derived small RNAs (tsRNAs) are novel class noncoding implicated in various human diseases. However, the role mechanism tsRNAs regulating trophoblast function during RSA development remain unknown. High-throughput sequencing was performed analyze differential villous tissues patients with controls. CCK-8, transwell assay, flow cytometry were detect effects tRF-5028c on proliferation, migration, invasion, apoptosis extravillous cell line HTR-8/SVneo. The target genes predicted via bioinformatic analysis verified by dual luciferase reporter gene assay. Moreover, pregnant mice injected mimics confirm findings vivo. A total 1907 detected, which 298 differentially expressed tissues. significantly upregulated group compared control. Functionally, overexpression inhibited HTR-8/SVneo invasion promoted apoptosis, whereas knockdown showed opposite effects. Mechanically, suppressed CRKL expression directly binding its 3'-untranslated region, thus inactivating downstream C3G/Rap1 signaling pathway. Finally, injection increased embryo absorption rate mice. upregulation impaired facilitate targeting CRKL-mediated Rap1 provide first evidence tsRNA dysregulation pathogenesis lay foundation for potential targeted therapies.

Language: Английский

Citations

1
Methylation modifications in tRNA and associated disorders: Current research and potential therapeutic targets DOI Creative Commons
Zhijing Wu, Ruixin Zhou,

Baizao Li

et al.

Cell Proliferation, Journal Year: 2024, Volume and Issue: 57(9)

Published: June 28, 2024

High-throughput sequencing has sparked increased research interest in RNA modifications, particularly tRNA methylation, and its connection to various diseases. However, the precise mechanisms underpinning development of these diseases remain largely elusive. This review sheds light on roles several methylations (m1A, m3C, m5C, m1G, m2G, m7G, m5U, Nm) diverse biological functions, including metabolic processing, stability, protein interactions, mitochondrial activities. It further outlines linked aberrant related enzymes, potential underlying mechanisms. Moreover, disruptions regulation abnormalities tRNA-derived small RNAs (tsRNAs) contribute disease pathogenesis, highlighting their as biomarkers for diagnosis. The also delves into exploration drugs targeting methylation emphasizing therapeutic prospects modulating processes. Continued is imperative a comprehensive comprehension integration molecular diagnosis treatment.

Language: Английский

Citations

4
The Mechanisms, Research Status, and Future Prospects of m6A Modification in Breast Cancer DOI Creative Commons

Xiu Xue‐mei,

Chen Yang,

Ju Wen‐ting

et al.

The Journal of Gene Medicine, Journal Year: 2025, Volume and Issue: 27(2)

Published: Feb. 1, 2025

ABSTRACT N6‐methyladenosine (m6A) modification is a significant methylation alteration frequently observed in eukaryotic RNAs, garnering considerable attention the field of breast cancer research recent years. The m6A profoundly influences onset, progression, and prognosis by regulating RNA stability, translation efficiency, degradation processes. Numerous studies have demonstrated that regulatory factors, including METTL3, METTL14, ALKBH5, play pivotal roles cells, affecting cell proliferation, metastasis, drug resistance. Furthermore, interactions between non‐coding as well its role tumor microenvironment, increasingly attracted researchers' interest. Although numerous elucidated dual cancer, specific molecular mechanisms remain to be thoroughly investigated. Future should explore various aspects, different subtypes involvement chemotherapy resistance, with microenvironment. This exploration will contribute advancements diagnosis treatment cancer. present article aims systematically summarize progress on offering novel insights strategies for future related clinical applications.

Language: Английский

Citations

0