Microbial Ecology, Год журнала: 2016, Номер 73(1), С. 201 - 210
Опубликована: Ноя. 21, 2016
Язык: Английский
Current Biology, Год журнала: 2019, Номер 29(11), С. R485 - R495
Опубликована: Июнь 1, 2019
Язык: Английский
Процитировано
209
Nature Communications, Год журнала: 2017, Номер 8(1)
Опубликована: Ноя. 24, 2017
Plants respond to insect attack by releasing blends of volatile chemicals that attract their herbivores' specific natural enemies, while herbivores may carry endosymbiotic microorganisms directly improve herbivore survival after enemy attack. Here we demonstrate the two phenomena can be linked. fed upon pea aphids release volatiles parasitic wasps, and aphid facultative bacteria prevent development wasp larva thus markedly We show these endosymbionts also attenuate systemic plants attack, reducing recruitment increasing fitness. Our results reveal a novel mechanism through which symbionts benefit hosts emphasise importance considering microbiome in understanding ecological interactions.
Язык: Английский
Процитировано
117
Molecular Ecology, Год журнала: 2016, Номер 26(8), С. 2363 - 2378
Опубликована: Ноя. 16, 2016
Symbiotic associations with bacteria have facilitated important evolutionary transitions in insects and resulted long-term obligate interactions. Recent evidence suggests that these are not always evolutionarily stable symbiont replacement, and/or supplementation of an symbiosis by additional bacterium, has occurred during the history many insect groups. Yet, factors favouring one over another this dynamic well understood; progress been hindered our incomplete understanding distribution symbionts across phylogenetic ecological contexts. While aphids engaged into a single Gammaproteobacterium, Buchnera aphidicola, species Lachninae subfamily, relationship evolved 'ménage à trois', which is complemented cosymbiont, usually Serratia symbiotica. Using deep sequencing 16S rRNA bacterial genes from 128 Cinara (the most diverse genus), we reveal highly dual symbiotic system aphid lineage. Most host both but, several clades, endosymbionts related to Sodalis, Erwinia or unnamed member Enterobacteriaceae replaced Serratia. Endosymbiont genome sequences four confirm coresident fulfil essential metabolic functions ensured Buchnera. We further demonstrate through comparative analyses cosymbiont replacement associated adaptation new conditions. propose succession was driven intrinsic phenomenon endosymbiosis, such as rapid deterioration competitive interactions between similar capabilities.
Язык: Английский
Процитировано
104
Proceedings of the Royal Society B Biological Sciences, Год журнала: 2017, Номер 284(1866), С. 20171925 - 20171925
Опубликована: Ноя. 1, 2017
Heritable symbionts are common in insects with many contributing to host defence. Hamiltonella defensa is a facultative, bacterial symbiont of the pea aphid, Acyrthosiphon pisum that provides protection against endoparasitoid wasp Aphidius ervi Protection levels vary among strains H. differentially infected by bacteriophages named APSEs. By contrast, little known about mechanism(s) resistance owing intractability host-restricted microbes for functional study. Here, we developed methods culturing varied presence and type APSE. Most proliferated at 27°C co-cultures TN5 cell line or as pure cultures no insect cells. The strain APSE3, which high vivo, produced soluble factor(s) disabled development A. embryos independent any aphid factors. Experimental transfer APSE3 also conferred ability disable phage-free Altogether, these results provide critical foundation characterizing symbiont-derived involved other functions. Our demonstrate phage-mediated traits mechanism innovation restricted symbionts.
Язык: Английский
Процитировано
89
GigaScience, Год журнала: 2019, Номер 8(4)
Опубликована: Апрель 1, 2019
Abstract Background The corn leaf aphid (Rhopalosiphum maidis Fitch) is the most economically damaging pest on maize (Zea mays), one of world's important grain crops. In addition to causing direct damage by removing photoassimilates, R. transmits several destructive viruses, including yellow dwarf virus, barley sugarcane mosaic and cucumber virus. Findings genome a parthenogenetically reproducing clone was assembled with combination Pacific Biosciences (207-fold coverage) Illumina (83-fold sequencing. 689 contigs, which have an N50 size 9.0 megabases (Mb) low level heterozygosity, were clustered using Phase Genomics Hi-C interaction maps. Consistent commonly observed 2n = 8 karyotype maidis, contigs (473 spanning 321 Mb) successfully oriented into 4 scaffolds. assembly captured full length 95.8% core eukaryotic genes, indicating that it highly complete. Repetitive sequences accounted for 21.2% assembly, total 17,629 protein-coding genes predicted integrated evidence from ab initio homology-based gene predictions transcriptome generated both Illumina. An analysis likely horizontally transferred identified 2 bacteria, 7 fungi, protozoa, 9 algae. Repeat elements, transposons, encoding detoxification enzymes (cytochrome P450s, glutathione S-transferases, carboxylesterases, uridine diphosphate–glucosyltransferases, ABC transporters) in sequence. Other than Buchnera aphidicola (642,929 base pairs, 602 genes), no endosymbiont bacteria found maidis. Conclusions A high-quality at chromosome level. This sequence will enable further research related ecological interactions, virus transmission, pesticide resistance, other aspects biology. It also serves as valuable resource comparative investigation species.
Язык: Английский
Процитировано
80
Molecular Ecology, Год журнала: 2021, Номер 30(11), С. 2483 - 2494
Опубликована: Март 23, 2021
Abstract Many animals have evolved associations with symbiotic microbes that benefit the host through increased growth, lifespan, and survival. Some interactions are obligate (essential for survival) while others facultative (usually beneficial but not essential). Not all individuals symbionts in a population, thus there is probably trade‐off between cost of hosting these benefits they confer to host. Plant‐sucking insects been one most important models test costs experimentally. This research now moving beyond description symbiont effects towards understanding mechanisms action, their role wider ecological community. We present quantitative systematic analysis published evidence exploring this question. found whitefly true bugs experience growth fecundity, whereas aphids fecundity resistance natural enemies. also report lack data some plant‐sucking groups, explore variation effect strengths directions across aphid host, plant species highlighting importance considering context dependency interactions.
Язык: Английский
Процитировано
70
Current Opinion in Insect Science, Год журнала: 2018, Номер 32, С. 1 - 7
Опубликована: Авг. 30, 2018
Язык: Английский
Процитировано
83
Philosophical Transactions of the Royal Society B Biological Sciences, Год журнала: 2016, Номер 371(1702), С. 20150325 - 20150325
Опубликована: Авг. 2, 2016
Recent research has shown that the bacterial endosymbionts of insects are abundant and diverse, they have numerous different effects on their hosts' biology. Here we explore how insect might affect structure dynamics communities. Using obligate facultative symbionts aphids as an example, find there multiple ways symbiont presence food web structure. Many now known to help hosts escape or resist natural enemy attack, others can allow withstand abiotic stress host plant use. In addition direct effect aphid phenotypes may be indirect mediated through trophic non-trophic community interactions. We believe by using data from barcoding studies identify symbionts, this extra, microbial dimension webs better elucidated.This article is part themed issue 'From DNA barcodes biomes'.
Язык: Английский
Процитировано
80
Ecology, Год журнала: 2016, Номер 97(7), С. 1712 - 1723
Опубликована: Май 25, 2016
It has become increasingly evident that many organisms rely on microbial symbionts for defense against natural enemies, but the ecological importance of defensive communities still needs to be investigated. A well-known example is Hamiltonella defensa, a heritable endosymbiotic bacterium commonly found in aphids. Laboratory experiments have shown H. defensa strongly protects aphids parasitic wasps (parasitoids), although this protection not equally effective different species parasitoids, or even genotypes same species. These results suggest plays an important role reducing aphid mortality by parasitoids and presumably affects community composition relying as resource. However, there little evidence indeed case under conditions. We tested field experiment with black bean (Aphis fabae) setting up replicated plots genetically identical did harbor following their colonization enemies over growing season. observed clear reduction parasitism symbiont-protected aphids, particularly posing highest risk. protected develop larger populations than unprotected ones, possibly reflecting balancing effect costs associated harboring defensa. also shifts parasitoid showing potential alter diversity structure food webs, likely consequences function stability.
Язык: Английский
Процитировано
70
Applied and Environmental Microbiology, Год журнала: 2019, Номер 86(5)
Опубликована: Дек. 17, 2019
Animal-associated microbes are highly variable, contributing to a diverse set of symbiont-mediated phenotypes. Given that host and symbiont genotypes, their interactions, can impact symbiont-based phenotypes across environments, there is potential for extensive variation in fitness outcomes. Pea aphids, Acyrthosiphon pisum, assemblage heritable facultative symbionts (HFS) with characterized roles defense. Protective have been largely studied as single infections, but pea aphids often carry multiple HFS species, particular combinations may be enriched or depleted compared expectations based on chance. Here, we examined the consequences infection versus coinfection two common exhibiting variable enrichment, antiparasitoid Hamiltonella defensa antipathogen Regiella insecticola, three genotypes environments. As expected, infections either H. R. insecticola raised defenses against respective targets. Single protective lowered aphid absence enemy challenge, while was comparatively benign. However, coinfection, ameliorated costs. Coinfected continued receive protection from defensa, weakened by clones. Notably, eliminated survival benefits conferred after pathogen exposure coinfecting Since sporulation suppressed coinfected poor performance likely stemmed defensa-imposed costs rather than defenses. Our results reveal complex outcomes which partially explain natural patterns suggest not easily predicted solely status.IMPORTANCE The hyperdiverse arthropods harbor maternally transmitted bacteria protect enemies. In many low-diversity communities common, providing opportunities cooperation conflict among symbionts, defensive services rendered. Using aphid, model symbiosis, show coinfections antiparasite Hamiltonella, produce consistently designated Compared reduced during challenge yet improved Thus, does necessarily create generalist "Swiss army knife" numerous Instead, favored variety reasons, including abilities lessen other when enemies present.
Язык: Английский
Процитировано
59