Psychological Stress, Its Reduction, and Long-Term Consequences: What Studies with Laboratory Animals Might Teach Us about Life in the Dog Shelter

Animals, Год журнала: 2020, Номер 10(11), С. 2061 - 2061

Опубликована: Ноя. 7, 2020

There is a long history of laboratory studies the physiological and behavioral effects stress, its reduction, later psychological consequences unmitigated stress responses. Many stressors employed in these approximate experience dogs confined an animal shelter. We review how literature has guided our own work describing reactions to shelter housing helping formulate means reducing their Consistent with social buffering other species, human interaction emerged as key ingredient moderating glucocorticoid responses dogs. discuss variables that appear critical for effective use procedures well potential neural mechanisms underlying glucocorticoid-reducing effect. also describe recent which enrichment centered on been found reduce aggressive temperament test used determine suitability adoption. Finally, we suggest aspect underappreciated studying evidence long-term consequences—often mediated by glucocorticoids—that may not become apparent until after initial exposure.

Язык: Английский

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Estrogen Receptor β Contributes to Both Hypertension and Hypothalamic Plasticity in a Mouse Model of Peri-Menopause

Journal of Neuroscience, Год журнала: 2021, Номер 41(24), С. 5190 - 5205

Опубликована: Май 3, 2021

Hypertension susceptibility in women increases at the transition to menopause, termed perimenopause, a state characterized by erratic estrogen fluctuation and extended hormone cycles. Elucidating role of signaling emergence hypertension during perimenopause has been hindered animal models that are confounded abrupt cessation or effects aging. In present study, accelerated ovarian failure (AOF) receptor β (ERβ) reporter mice was induced 4-vinylcyclohexene diepoxide young model early-stage (peri-AOF) characteristic peri-menopause. It found administering ERβ agonists suppressed elevated blood pressure neurogenic angiotensin II (AngII) peri-AOF, but not age-matched male mice. also agonist administration peri-AOF females, males, heightened NMDAR reactive oxygen production neurons hypothalamic paraventricular nucleus (PVN), critical neural regulator pressure. further shown deleting PVN gonadally intact females produced phenotype marked sensitivity AngII hypertension. These results suggest plays an important regulation female contributes early stage comparable human perimenopause.

Язык: Английский

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Diurnal effects of polypharmacy with high drug burden index on physical activities over 23 h differ with age and sex

Scientific Reports, Год журнала: 2022, Номер 12(1)

Опубликована: Фев. 9, 2022

Aging, polypharmacy (concurrent use of ≥ 5 medications), and functional impairment are global healthcare challenges. However, knowledge the age/sex-specific effects is limited, particularly on daily physical activities. Using continuous monitoring, we demonstrated how with high Drug Burden Index (DBI-cumulative anticholinergic/sedative exposure) affected behaviors over 23 h in male/female, young/old mice. For comparison, also evaluated different drug regimens (polypharmacy/monotherapy) influenced activities young We found that after 4 weeks treatment, DBI (HDBI) decreased exploration (reduced mean gait speed climbing) during habituation period, but increased it other periods, old mice transition to inactivity. After HDBI polypharmacy, consistently throughout experiment. Some behavioral declines were more marked females than males, indicating treatment × sex interactions. Metoprolol simvastatin monotherapies mice, compared control/polypharmacy. These findings highlight some polypharmacy-associated changes greater age females. The observed diurnal analogous drug-induced delirium sundowning seen older adults. Future mechanistic investigations needed further inform considerations age, sex, optimize quality medicines.

Язык: Английский

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A single reminder trial updates fear memory and affects extinction in rats housed under different living conditions

Learning and Motivation, Год журнала: 2025, Номер 89, С. 102098 - 102098

Опубликована: Янв. 31, 2025

Язык: Английский

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Risk factors for barbering in laboratory mice

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Март 3, 2025

Barbering is a common abnormal behavior in laboratory mice, where mice pluck their own fur and/or the or whiskers of cage mates. are concern for welfare and research quality, as well serving spontaneous model trichotillomania (a hair-pulling disorder humans). Causes prevention barbering poorly understood, although there evidence that both biological environmental factors play role its prevalence. Since initial work this area was done 20 years ago, mouse husbandry has changed dramatically. We provide an updated analysis risk based on point prevalence hair loss 2544 cages over one year (7007 mice). analyzed effects biological, environmental, known to be stressors mice. found certain barbering, such sex breeding status, have persisted despite changes housing. additionally identified differences genetic background, housing system, time year, "hotspot" effect showing spatial clustering barbering. Our findings can used increase understanding inform reduce

Язык: Английский

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Voluntary wheel exercise improves learning and memory impairment caused by hippocampal Hb-α deficiency by reducing microglial activation and reversing synaptic damage

Brain Behavior and Immunity, Год журнала: 2025, Номер 127, С. 81 - 95

Опубликована: Март 7, 2025

Язык: Английский

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Oxytocin administration rescues the negative impacts of social isolation on wound healing in mice

Hormones and Behavior, Год журнала: 2025, Номер 171, С. 105741 - 105741

Опубликована: Апрель 15, 2025

Язык: Английский

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Pharmacological agents and injection stress, but not social isolation, alter cognitive judgement bias in the mouse touchscreen operant chamber

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Апрель 21, 2025

Cognitive judgement bias (CJB) refers to the interpretation of ambiguous stimuli in a negative (pessimistic) or positive (optimistic) way. Negative CJB is observed depression and anxiety, conditions that burden affected individuals their caregivers. Pre-clinical animal research key understanding developing therapies for affective anxiety disorders, so translationally relevant test would be useful addition existing pre-clinical rodent touchscreen battery. Here, task mouse operant chambers was adapted validated using pharmacological agents, bupropion tetrabenazine, known induce CJB, respectively. Further, shifts were following an injection procedure (handling intraperitoneal with saline solution), demonstrating sensitivity task. Interestingly, whereas social isolation has been linked changes state humans, 8 weeks single-housing mice did not alter but anxiety-like behaviour open field. Collectively, these results present assess mice, which will facilitate future explorations into underlying neurobiology potential treatment disorders.

Язык: Английский

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SOSYAL İZOLASYONUN NEDEN OLDUĞU STRESİN OKSİDATİF STRES BELİRTEÇLERİ VE ÖĞRENME İLE İLİŞKİLİ NÖROGRANİN/ CAMKII/CREB PROTEİNLERİNE ETKİSİ

Kocatepe tıp dergisi., Год журнала: 2025, Номер 26(2), С. 148 - 155

Опубликована: Апрель 28, 2025

AMAÇ: Sosyal izolasyon (Sİ) hem insanlar için de ratlar gibi sosyal hayvanlar önemli bir stres faktörüdür. Sİ stresine maruz kalan ratların davranışsal bozukluklar gösterdiği ve kognitif fonksiyonlarının bozulduğu bilinmektedir. Bu çalışmanın amacı erken dönemde oluşturulan stresinin öğrenme hafızayı nasıl etkilediğini sinaptik proteinlerde meydana gelen değişiklikleri incelemektir. GEREÇ VE YÖNTEM: Çalışmada 21 günlük 20 adet erkek Wistar albino cinsi rat kullanıldı. Ratlar rastgele 2 gruba ayrıldı: 1) Kontrol grubu (n=10), 2) (n=10). doğum sonrası 21. günde sütten kesmenin ilk gününden itibaren 6 hafta boyunca her sıçanın farklı kafeslerde barındırılmasıyla gerçekleştirildi. hayvanları ise kafeste 3-4 sıçan olacak şekilde etkileşimin gerçekleşeceği standart koşullarda barındırıldı. haftalık süre sonunda değerlendirmek Morris su labirent (MWM) testi Test feda edilerek hipokampüs dokuları toplandı. Doku malondialdehid (MDA) indirgenmiş glutatyon (GSH) seviyeleri spektrofotometrik olarak nörogranin, kalsiyum/kalmodulin bağımlı protein kinaz tip II (CaMKII), Döngüsel AMP (cAMP) cevap elementine bağlanan (CREB) Beyinden türeyen nörotrofik faktör (BDNF) ELISA ile değerlendirildi. BULGULAR: kontrol arasında MWM testinde hafıza açısından fark saptanmadı. Hipokampüs dokusunda CaMKII CREB düzeyleri gruplar değildi. yapılan grupta MDA düzeyi artarken, GSH BDNF azaldı. SONUÇ: Çalışmamızda kesme altı bozukluğuna neden olmadan hipokampüste oksidatif stresi arttırdığı, düzeylerini düşürdüğü diğer proteinleri değiştirmediği gösterildi.

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A depression–like phenotype is associated with discrete defects in the primary hippocampal circuit.

Опубликована: Май 30, 2025

Major depressive disorder is known to disturb the hippocampus, but how this impacts signal processing performed by structure remains poorly understood. Here, we report that single housing (7–10 days) promotes a depression-like phenotype in young adult mice associated with robust, yet surprisingly discreet defect information flow across primary hippocampal circuit. In addition perturbations social and despair-like behavior, eliminated preference for novelty impaired episodic memory encoding. Additionally, lateral habenula, an epithalamic critically involved depression, was hyperactive. Although complex CA1 response generated single-pulse perforant path (LPP) activation of hippocampus largely unaffected housing, pronounced differences emerged when circuit activated physiologically relevant frequencies patterns. The characteristic ‘theta/gamma’ pattern distorted such facilitation observed single-housed slices, while filtering output brief beta (25Hz) gamma (50Hz) frequency LPP stimulation evident group housed slices absent. A novel filter within field CA3 suppressed spike (but not synaptic potentials), subsequent propagation CA1, inputs those arriving at frequencies. This significantly following housing. These results suggest depression highly selective partial loss inhibition links circuit, providing new insights into relationship between function.

Язык: Английский

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