Effects of single housing on behavior, corticosterone level and body weight in male and female mice

Laboratory Animal Research, Journal Year: 2024, Volume and Issue: 40(1)

Published: Sept. 29, 2024

Abstract Background Experimental mice are often single-housed either for an individual analysis (feeding behavior, imaging, calorimetry) or as a stress paradigm (social isolation) in translational biomedical research. Reports of the influence single housing rodents conflicting and may depend on age duration isolation. Sex is not included factor. In this study we investigated effects 4-week male female body weight, serum corticosterone levels. Results Behavioral tests showed no effect anhedonia coping, anxiety motor exploration. Social avoidance occurred both males females. Regarding physiological effects, did induce changes levels, but reduced weight gain. Conclusions While some mouse studies chronic social isolation reported depression-related disturbances, our data suggest that might be necessarily too stressful. This important animal welfare regulations experiments life science

Language: Английский

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A single reminder trial updates fear memory and affects extinction in rats housed under different living conditions

Learning and Motivation, Journal Year: 2025, Volume and Issue: 89, P. 102098 - 102098

Published: Jan. 31, 2025

Language: Английский

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Oxytocin administration rescues the negative impacts of social isolation on wound healing in mice

Hormones and Behavior, Journal Year: 2025, Volume and Issue: 171, P. 105741 - 105741

Published: April 15, 2025

Language: Английский

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SOSYAL İZOLASYONUN NEDEN OLDUĞU STRESİN OKSİDATİF STRES BELİRTEÇLERİ VE ÖĞRENME İLE İLİŞKİLİ NÖROGRANİN/ CAMKII/CREB PROTEİNLERİNE ETKİSİ

Kocatepe tıp dergisi., Journal Year: 2025, Volume and Issue: 26(2), P. 148 - 155

Published: April 28, 2025

AMAÇ: Sosyal izolasyon (Sİ) hem insanlar için de ratlar gibi sosyal hayvanlar önemli bir stres faktörüdür. Sİ stresine maruz kalan ratların davranışsal bozukluklar gösterdiği ve kognitif fonksiyonlarının bozulduğu bilinmektedir. Bu çalışmanın amacı erken dönemde oluşturulan stresinin öğrenme hafızayı nasıl etkilediğini sinaptik proteinlerde meydana gelen değişiklikleri incelemektir. GEREÇ VE YÖNTEM: Çalışmada 21 günlük 20 adet erkek Wistar albino cinsi rat kullanıldı. Ratlar rastgele 2 gruba ayrıldı: 1) Kontrol grubu (n=10), 2) (n=10). doğum sonrası 21. günde sütten kesmenin ilk gününden itibaren 6 hafta boyunca her sıçanın farklı kafeslerde barındırılmasıyla gerçekleştirildi. hayvanları ise kafeste 3-4 sıçan olacak şekilde etkileşimin gerçekleşeceği standart koşullarda barındırıldı. haftalık süre sonunda değerlendirmek Morris su labirent (MWM) testi Test feda edilerek hipokampüs dokuları toplandı. Doku malondialdehid (MDA) indirgenmiş glutatyon (GSH) seviyeleri spektrofotometrik olarak nörogranin, kalsiyum/kalmodulin bağımlı protein kinaz tip II (CaMKII), Döngüsel AMP (cAMP) cevap elementine bağlanan (CREB) Beyinden türeyen nörotrofik faktör (BDNF) ELISA ile değerlendirildi. BULGULAR: kontrol arasında MWM testinde hafıza açısından fark saptanmadı. Hipokampüs dokusunda CaMKII CREB düzeyleri gruplar değildi. yapılan grupta MDA düzeyi artarken, GSH BDNF azaldı. SONUÇ: Çalışmamızda kesme altı bozukluğuna neden olmadan hipokampüste oksidatif stresi arttırdığı, düzeylerini düşürdüğü diğer proteinleri değiştirmediği gösterildi.

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Age-Related Alterations in Brain Perfusion, Venous Oxygenation, and Oxygen Metabolic Rate of Mice: A 17-Month Longitudinal MRI Study

Frontiers in Neurology, Journal Year: 2020, Volume and Issue: 11

Published: June 12, 2020

Background: Characterization of physiological parameters the aging brain, such as perfusion and brain metabolism, is important for understanding function diseases. Aging studies on human have mostly been based cross-sectional design, while few longitudinal used relatively short follow-up time compared to lifespan. Objectives: To determine courses cerebral across adult lifespan in mice. Methods: The present work examined changes blood flow (CBF), venous oxygenation (Yv), metabolic rate oxygen (CMRO2) using MRI healthy C57BL/6 mice from 3 20 months age. Each mouse received 16 imaging sessions at an approximately 1-month interval. Results: Significant increases with age were observed CBF (p=0.017) CMRO2 (p<0.001). Meanwhile, Yv revealed a significant decrease (p=0.002) non-linear pattern (p=0.013). change was 0.87%, 2.26%, -0.24% per month CBF, CMRO2, Yv, respectively. On other hand, systemic heart did not show dependence (p=0.47). No white-matter-hyperintensities (WMH) T2-weighted image any Conclusion: With age, increase consumption. This observation consistent previous findings humans design suggests degradation brain's energy production or utilization machinery. Cerebral remains intact aged mice, least until absence WMH

Language: Английский

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Psychological Stress, Its Reduction, and Long-Term Consequences: What Studies with Laboratory Animals Might Teach Us about Life in the Dog Shelter

Animals, Journal Year: 2020, Volume and Issue: 10(11), P. 2061 - 2061

Published: Nov. 7, 2020

There is a long history of laboratory studies the physiological and behavioral effects stress, its reduction, later psychological consequences unmitigated stress responses. Many stressors employed in these approximate experience dogs confined an animal shelter. We review how literature has guided our own work describing reactions to shelter housing helping formulate means reducing their Consistent with social buffering other species, human interaction emerged as key ingredient moderating glucocorticoid responses dogs. discuss variables that appear critical for effective use procedures well potential neural mechanisms underlying glucocorticoid-reducing effect. also describe recent which enrichment centered on been found reduce aggressive temperament test used determine suitability adoption. Finally, we suggest aspect underappreciated studying evidence long-term consequences—often mediated by glucocorticoids—that may not become apparent until after initial exposure.

Language: Английский

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Brain metabolism in tau and amyloid mouse models of Alzheimer's disease: An MRI study

NMR in Biomedicine, Journal Year: 2021, Volume and Issue: 34(9)

Published: May 29, 2021

Alzheimer's disease (AD) is the leading cause of cognitive impairment and dementia in elderly individuals. According to current biomarker framework for “unbiased descriptive classification”, biomarkers neurodegeneration, “N”, constitute a critical component tri‐category “A/T/N” system. Current neurodegeneration suffer from potential drawbacks such as requiring invasive lumbar puncture, involving ionizing radiation, or representing late, irreversible marker. Recent human studies have suggested that reduced brain oxygen metabolism may be new functional marker AD, but heterogeneity presence mixed pathology patients did not allow full understanding role extraction AD. In this report, global related physiological parameters were studied two AD mouse models with relatively pure pathology, using advanced MRI techniques including T 2 ‐relaxation‐under‐spin‐tagging (TRUST) phase contrast (PC) MRI. Additionally, regional cerebral blood flow (CBF) was determined pseudocontinuous arterial spin labeling. Reduced fraction (by −18.7%, p = 0.008), unit‐mass metabolic rate (CMRO ) −17.4%, 0.04) total CMRO −30.8%, < 0.001) observed Tau4RΔK mice—referred tau model—which manifested pronounced measured by diminished volume −15.2%, 0.001). Global CBF these mice different those wild‐type ( > 0.05), suggesting normal vascular function. By contrast, B6;SJL‐Tg [APPSWE]2576Kha (APP) amyloid model—no reduction, well intact metabolism, found 0.05). Consistent imaging data, behavioral measures walking distance impaired 0.004), APP 0.88). Collectively, findings support hypothesis noninvasive measurement promising

Language: Английский

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Estrogen Receptor β Contributes to Both Hypertension and Hypothalamic Plasticity in a Mouse Model of Peri-Menopause

Journal of Neuroscience, Journal Year: 2021, Volume and Issue: 41(24), P. 5190 - 5205

Published: May 3, 2021

Hypertension susceptibility in women increases at the transition to menopause, termed perimenopause, a state characterized by erratic estrogen fluctuation and extended hormone cycles. Elucidating role of signaling emergence hypertension during perimenopause has been hindered animal models that are confounded abrupt cessation or effects aging. In present study, accelerated ovarian failure (AOF) receptor β (ERβ) reporter mice was induced 4-vinylcyclohexene diepoxide young model early-stage (peri-AOF) characteristic peri-menopause. It found administering ERβ agonists suppressed elevated blood pressure neurogenic angiotensin II (AngII) peri-AOF, but not age-matched male mice. also agonist administration peri-AOF females, males, heightened NMDAR reactive oxygen production neurons hypothalamic paraventricular nucleus (PVN), critical neural regulator pressure. further shown deleting PVN gonadally intact females produced phenotype marked sensitivity AngII hypertension. These results suggest plays an important regulation female contributes early stage comparable human perimenopause.

Language: Английский

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Diurnal effects of polypharmacy with high drug burden index on physical activities over 23 h differ with age and sex

Scientific Reports, Journal Year: 2022, Volume and Issue: 12(1)

Published: Feb. 9, 2022

Aging, polypharmacy (concurrent use of ≥ 5 medications), and functional impairment are global healthcare challenges. However, knowledge the age/sex-specific effects is limited, particularly on daily physical activities. Using continuous monitoring, we demonstrated how with high Drug Burden Index (DBI-cumulative anticholinergic/sedative exposure) affected behaviors over 23 h in male/female, young/old mice. For comparison, also evaluated different drug regimens (polypharmacy/monotherapy) influenced activities young We found that after 4 weeks treatment, DBI (HDBI) decreased exploration (reduced mean gait speed climbing) during habituation period, but increased it other periods, old mice transition to inactivity. After HDBI polypharmacy, consistently throughout experiment. Some behavioral declines were more marked females than males, indicating treatment × sex interactions. Metoprolol simvastatin monotherapies mice, compared control/polypharmacy. These findings highlight some polypharmacy-associated changes greater age females. The observed diurnal analogous drug-induced delirium sundowning seen older adults. Future mechanistic investigations needed further inform considerations age, sex, optimize quality medicines.

Language: Английский

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An Automated, Home-Cage, Video Monitoring-based Mouse Frailty Index Detects Age-associated Morbidity in C57BL/6 and Diversity Outbred Mice

The Journals of Gerontology Series A, Journal Year: 2023, Volume and Issue: 78(5), P. 762 - 770

Published: Jan. 27, 2023

Abstract Frailty indexes (FIs) provide quantitative measurements of nonspecific health decline and are particularly useful as longitudinal monitors morbidity in aging studies. For mouse studies, frailty assessments can be taken noninvasively, but they require handling direct observation that is labor-intensive to the scientist stress inducing animal. Here, we implement, evaluate, a refined digital FI composed entirely computational analyses home-cage video compare it manually obtained scores both C57BL/6 genetically heterogeneous Diversity Outbred mice. We show assigned by our index correlate with chronological age. Thus, an automated tool for assessment collected reproducibly, at scale, without substantial labor cost.

Language: Английский

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