Neotropical Entomology,
Год журнала:
2024,
Номер
53(4), С. 889 - 906
Опубликована: Май 7, 2024
Increased
attention
is
being
focused
on
the
biological
control
of
agricultural
pests
using
microorganisms,
owing
to
their
potential
as
a
viable
substitute
for
chemical
methods.
Insect
cadavers
constitute
source
entomopathogenic
microorganisms.
We
tested
whether
bacteria
and
fungi
isolated
from
Spodoptera
frugiperda
(JE
Smith)
could
affect
its
survival,
development,
egg-laying
pattern,
hatchability,
well
induce
mortality
in
Anthonomus
grandis
Boheman
adults.
Enterobacter
hormaechei
Serratia
marcescens
Scopulariopsis
sp.
Aspergillus
nomiae
fall
armyworm
pest
insects
were
subjected
an
artificial
diet
enriched
with
cells
or
fungal
spores
be
tested,
case
S.
frugiperda,
only
A.
grandis.
pathogenic
affecting
survival
adults
pupae.
The
fungus
does
not
caterpillars
pupae;
however,
due
late
action,
moths
eggs
may
affected.
also
increased
adults,
development
early
stages
exposure
diet,
indicated
by
vertical
spore
transfer
offspring
low
hatchability.
are
biocontrol
agents
these
pests,
warrant
further
investigation
toxicological
point
view
subsequently
field
tests
involving
formulations
that
improve
sustainability
practices.
Cell Reports,
Год журнала:
2022,
Номер
40(12), С. 111368 - 111368
Опубликована: Сен. 1, 2022
Quorum
sensing
fundamentally
alters
the
interaction
of
Vibrio
cholerae
with
aquatic
environments,
environmental
hosts,
and
human
intestine.
At
high
cell
density,
quorum-sensing
regulator
HapR
represses
not
only
expression
cholera
toxin
co-regulated
pilus,
virulence
factors
essential
in
infection,
but
also
synthesis
polysaccharide
(VPS)
exopolysaccharide-based
matrix
required
for
abiotic
biotic
surface
attachment.
Here,
we
describe
a
feature
V.
quorum
that
shifts
host-pathogen
toward
commensalism.
By
repressing
pathogen
consumptive
anabolic
metabolism
and,
particular,
tryptophan
uptake,
stimulates
host
intestinal
serotonin
production.
This,
turn,
activates
innate
immune
signaling
to
promote
survival.
PLoS Genetics,
Год журнала:
2023,
Номер
19(6), С. e1010782 - e1010782
Опубликована: Июнь 15, 2023
Endocrine
signaling
networks
control
diverse
biological
processes
and
life
history
traits
across
metazoans.
In
both
invertebrate
vertebrate
taxa,
steroid
hormones
regulate
immune
system
function
in
response
to
intrinsic
environmental
stimuli,
such
as
microbial
infection.
The
mechanisms
of
this
endocrine-immune
regulation
are
complex
constitute
an
ongoing
research
endeavor
facilitated
by
genetically
tractable
animal
models.
20-hydroxyecdysone
(20E)
is
the
major
hormone
arthropods,
primarily
studied
for
its
essential
role
mediating
developmental
transitions
metamorphosis;
20E
also
modulates
innate
immunity
a
variety
insect
taxa.
This
review
provides
overview
our
current
understanding
20E-mediated
responses.
prevalence
correlations
between
20E-driven
activation
summarized
range
holometabolous
insects.
Subsequent
discussion
focuses
on
studies
conducted
using
extensive
genetic
resources
available
Drosophila
that
have
begun
reveal
underlying
contexts
development
bacterial
Lastly,
I
propose
directions
future
into
will
advance
knowledge
how
interactive
endocrine
coordinate
animals'
physiological
responses
microbes.
Philosophical Transactions of the Royal Society B Biological Sciences,
Год журнала:
2024,
Номер
379(1901)
Опубликована: Март 18, 2024
Interactions
between
animals
and
microbes
are
ubiquitous
in
nature
strongly
impact
animal
physiology.
These
interactions
shaped
by
the
host
immune
system,
which
responds
to
infections
contributes
tailor
associations
with
beneficial
microorganisms.
In
many
insects,
symbiotic
not
only
include
gut
commensals,
but
also
intracellular
bacteria,
or
endosymbionts.
Endosymbionts
housed
within
specialized
cells,
bacteriocytes,
transmitted
vertically
across
generations.
Host–endosymbiont
co-evolution
shapes
endosymbiont
genome
fights
against
microbial
intruders,
ensures
preservation
of
endosymbionts
control
their
load
location.
The
cereal
weevil
Sitophilus
spp.
is
a
remarkable
model
study
evolutionary
adaptation
system
endosymbiosis
owing
its
binary
association
unique,
relatively
recently
acquired
nutritional
endosymbiont,
Sodalis
pierantonius
.
This
Gram-negative
bacterium
has
experienced
size
shrinkage
observed
long-term
endosymbioses
retained
immunogenicity.
We
focus
here
on
sixteen
antimicrobial
peptides
(AMPs)
identified
oryzae
expression
patterns
different
tissues,
along
development
upon
challenges,
address
potential
functions
defensive
response
homeostasis
insect
life
cycle.
article
part
theme
issue
‘Sculpting
microbiome:
how
factors
determine
respond
colonization’.
Abstract
Mosquitoes
are
prolific
vectors
of
human
pathogens,
therefore
a
clear
and
accurate
understanding
the
organization
their
antimicrobial
defenses
is
crucial
for
informing
development
transmission
control
strategies.
The
canonical
infection
response
in
insects,
as
described
insect
model
Drosophila
melanogaster
,
pathogen
type-dependent,
with
distinct
stereotypical
responses
to
Gram-negative
bacteria
Gram-positive
bacteria/fungi
mediated
by
activation
Imd
Toll
pathways,
respectively.
To
determine
whether
this
pathogen-specific
discrimination
shared
mosquitoes,
we
used
RNAseq
capture
genome-wide
transcriptional
Aedes
aegypti
Anopheles
gambiae
(
s.l.
)
systemic
bacteria,
yeasts,
filamentous
fungi,
well
challenge
heat-killed
Gram-negative,
Gram-positive,
fungal
pathogens.
From
resulting
data,
found
that
Ae.
An.
both
mount
core
all
categories
infection,
highly
conserved
between
two
species
respect
function
orthology.
When
compared
transcriptomes
mosquitoes
infected
different
types
observed
intensity
was
correlated
virulence
growth
rate
infecting
pathogen.
Exhaustive
comparisons
Gram-negative-challenged
versus
Gram-positive-challenged
yielded
no
difference
either
species.
In
however,
identified
signatures
specific
bacterial
infection.
dominated
expression
defensins
cecropins,
while
included
disproportionate
upregulation
an
uncharacterized
family
glycine-rich
proteins.
These
were
also
challenged
indicating
can
discriminate
molecular
patterns
fungi.
FEBS Journal,
Год журнала:
2023,
Номер
290(11), С. 2881 - 2894
Опубликована: Янв. 7, 2023
Juvenile
hormone
(JH)
signalling
provides
vital
regulatory
functions
during
insect
development
via
transcriptional
regulation
of
genes
critical
for
the
progression
metamorphosis
and
oogenesis.
Despite
importance
JH
signalling,
underlying
molecular
mechanisms
remain
largely
unknown.
Our
current
understanding
pathway
depends
on
static
end‐point
information
suffers
from
lack
time‐resolved
data.
Here,
we
have
addressed
dynamic
aspect
by
monitoring
in
real
time
interactions
receptor
proteins.
Use
two
tags
that
reconstitute
a
functional
luciferase
when
proximity
enabled
us
to
follow
rapid
assembly
heterodimer
basic
helix–loop–helix/Per‐Arnt‐SIM
(bHLH‐PAS)
proteins,
methoprene‐tolerant
(Met)
taiman
(Tai),
upon
specific
binding
Met.
On
similar
timescale
(minutes),
dissociation
Met‐Met
complexes
occurred,
again
strictly
dependent
Met
interaction
with
agonist
ligands.
To
resolve
questions
regarding
role
chaperone
Hsp90/83
JHR
complex
formation,
used
same
technique
demonstrate
Met‐Hsp83
persisted
absence
but
readily
dissociated
Preincubation
Hsp90
inhibitor
geldanamycin
showed
protected
degradation
was
produce
active
dimer
Tai.
Thus,
appear
be
governed
principles
those
regulating
aryl
hydrocarbon
receptor,
closest
vertebrate
homologue
arthropod
receptor.
Insects,
Год журнала:
2021,
Номер
12(5), С. 423 - 423
Опубликована: Май 8, 2021
The
immunocompetence
of
the
mealworm
beetle
Tenebrio
molitor
has
been
well
investigated
at
molecular
and
physiological
levels,
but
information
on
morphological
functional
characteristics
its
immune
cells
(haemocytes)
is
still
scarce
fragmentary.
This
study
provides
an
updated
overview
morphology
circulating
from
adults,
using
light
transmission
electron
microscopy.
Based
their
affinities
for
May–Grünwald
Giemsa
stain,
haemocytes
were
defined
as
either
eosinophilic,
basophilic
or
neutral.
Ultrastructural
descriptions
allowed
to
detect
four
main
cell
types
in
haemolymph:
prohaemocytes,
plasmatocytes,
granular
oenocytoids.
plasticity
evidence
mitotic
cells,
intermediate
stages,
autophagic
activities
suggest
haemocyte
proliferation,
turnover
transdifferentiation
constantly
active
processes
haemolymph.
Cytochemical
tests
revealed
differences
distribution
carbohydrates
among
underling
great
response
direct
involvement
resource
allocation.
In
addition,
our
results
provide
a
detailed
description
vesicle
trafficking,
macro-
microautophagy,
apoptotic
necrotic
processes,
confirming
suitability
T.
model
studying
evolutionarily
conserved
cellular
mechanisms.
Insects,
Год журнала:
2025,
Номер
16(1), С. 54 - 54
Опубликована: Янв. 8, 2025
Insect
diapause
and
response
to
thermal
stress
are
similar
in
the
variety
of
manifestations.
However,
influence
shocks
on
incidence
insect
has
not
been
sufficiently
studied.
Our
laboratory
experiments
showed
that
both
cold
(-10
°C)
heat
(43
experienced
for
at
least
20-30
min
significantly
reduced
facultative
larval
winter
egg
parasitoid
Trichogramma
telengai.
patterns
these
responses
were
substantially
different.
In
particular,
peaks
sensitivity
diapause-averting
effects
fell,
correspondingly,
middle-stage
(5
days
development
15
late-stage
(9-11
larvae.
Heat
mostly
via
changes
initial
proportions
diapause-destined
non-diapause-destined
individuals,
whereas
effect
is
based
differential
mortality
(i.e.,
difference
among
treatments
same
experiment)
with
better
survival
individuals.
These
results
elucidate
peculiarities
interaction
between
diapause,
allowing
us
specify
methods
mass
rearing
storage.
Insects,
Год журнала:
2025,
Номер
16(3), С. 311 - 311
Опубликована: Март 17, 2025
Insects
face
the
constant
challenge
of
balancing
energy
allocation
between
reproduction
and
immune
responses,
both
which
are
highly
energy-demanding
processes.
Immune
challenges
frequently
result
in
decreased
fecundity,
reduced
egg
viability,
delayed
ovarian
development.
Conversely,
heightened
reproductive
activity
often
suppresses
functions.
This
trade-off
has
profound
ecological
evolutionary
consequences,
shaping
insects’
survival,
adaptation,
population
dynamics.
The
intricate
interplay
immunity
insects
is
regulated
by
neuroendocrine
endocrine
systems,
orchestrate
resource
distribution
alongside
other
biological
Key
hormones,
such
as
juvenile
hormone
ecdysteroids,
serve
central
regulators,
influencing
responses
activities.
Additionally,
macromolecules
like
vitellogenin
lipophorin,
primarily
known
for
their
functions
yolk
protein
precursors
lipid
carriers,
play
crucial
roles
pathogen
recognition
transgenerational
priming.
Advancements
molecular
omics
tools
have
unveiled
complexity
these
regulatory
mechanisms,
providing
new
insights
into
how
dynamically
allocate
resources
to
optimize
fitness.
delicate
balance
underscores
critical
strategies
integration
physiological
systems
across
species.
review
synthesizes
from
life
history
theory,
oogenesis,
immunity,
offering
perspectives
on
trade-offs
output
investment.
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(6), С. 2786 - 2786
Опубликована: Март 19, 2025
As
a
tropomyosin-binding
component,
troponin
T
(TnT)
is
essential
for
the
Ca2+
regulation
of
striated
muscles’
contraction
and
locomotion
activity,
but
its
impacts
on
growth
development
insects
have
rarely
been
reported.
In
this
study,
TnT
was
identified
functionally
characterized
in
Tribolium
castaneum
by
RNA
interference
(RNAi)
transcriptome
analysis.
The
T.
contained
1152
bp
open
reading
frame
encoding
383
amino
acids.
It
displayed
highest
expression
late
pupae
highly
expressed
integument
CNS.
Both
larval
early
pupal
injection
dsTnT
led
to
100%
cumulative
mortality
before
pupal–adult
transition.
Late
RNAi
caused
26.01
±
4.29%
mortality;
survivors
successfully
became
adults,
49.71
6.51%
died
10
days
with
dried
shriveled
abdomen,
poorly
developed
reproductive
system
no
offspring.
Additionally,
sequencing
results
indicated
that
key
ecdysteroid
juvenile
hormone
biosynthesis
genes
(CYP314A1,
aldehyde
dehydrogenase
family
3
member
B1
farnesol
dehydrogenase)
were
affected,
as
well
several
cuticle
protein,
nutrition
metabolism
immune-related
genes,
suggesting
may
play
prominent
roles
development,
reproduction
affecting
these
pathways.
This
study
could
provide
brand-new
target
gene
strategy
pest
control.
