Animal Bioscience,
Год журнала:
2024,
Номер
38(3), С. 444 - 453
Опубликована: Окт. 24, 2024
Objective:
Expanded
potential
stem
cells
(EPSCs)
are
that
can
differentiate
into
embryonic
and
extraembryonic
lineages,
including
endoderm
trophoblast
lineages.
Therefore,
EPSCs
have
great
in
advancing
regenerative
medicine,
elucidating
disease
mechanisms,
exploring
early
development.
However,
the
generation
characterization
of
pigs
not
been
thoroughly
explored.
In
this
study,
we
successfully
generated
porcine
(pEPSCs).Methods:
We
reprogrammed
fetal
fibroblasts
(PFFs)
using
an
integration-free
method
with
Sendai
virus
vectors.Results:
The
resulting
pEPSCs
expressed
key
pluripotency
markers
demonstrated
ability
to
between
Notably,
reprogramming
was
associated
a
transformation
mitochondrial
morphology
from
elongated
form
observed
PFFs
globular
shape,
reflecting
alterations
energy
metabolism.
significant
remodeling
subsequent
shift
towards
glycolytic
dependence
during
pEPSCs.Conclusion:
Our
findings
provide
valuable
insights
characteristics
highlight
their
applications
modeling,
emerging
fields
such
as
cell-based
meat
production.
Cell stem cell,
Год журнала:
2024,
Номер
31(3), С. 312 - 333
Опубликована: Фев. 20, 2024
Though
totipotency
and
pluripotency
are
transient
during
early
embryogenesis,
they
establish
the
foundation
for
development
of
all
mammals.
Studying
these
in
vivo
has
been
challenging
due
to
limited
access
ethical
constraints,
particularly
humans.
Recent
progress
led
diverse
culture
adaptations
epiblast
cells
vitro
form
totipotent
pluripotent
stem
cells,
which
not
only
deepen
our
understanding
embryonic
but
also
serve
as
invaluable
resources
animal
reproduction
regenerative
medicine.
This
review
delves
into
hallmarks
shedding
light
on
their
key
molecular
functional
features.
Scientific Reports,
Год журнала:
2021,
Номер
11(1)
Опубликована: Май 26, 2021
Bovine
embryonic
stem
cells
(bESCs)
extend
the
lifespan
of
transient
pluripotent
bovine
inner
cell
mass
in
vitro.
After
years
research,
derivation
stable
bESCs
was
only
recently
reported.
Although
successful,
bESC
culture
relies
on
complex
conditions
that
require
a
custom-made
base
medium
and
mouse
fibroblasts
(MEF)
feeders,
limiting
widespread
use
bESCs.
We
report
here
simplified
based
replacing
custom
with
commercially
available
alternative
eliminating
need
for
MEF
feeders
by
using
chemically-defined
substrate.
lines
were
cultured
derived
consisting
N2B27
supplements
1%
BSA
(NBFR-bESCs).
Newly
easy
to
establish,
simple
propagate
after
long-term
culture.
These
expressed
pluripotency
markers
actively
proliferated
more
than
35
passages
while
maintaining
normal
karyotype
ability
differentiate
into
derivatives
all
three
germ
lineages
embryoid
bodies
teratomas.
In
addition,
NBFR-bESCs
grew
multiple
feeder-free
system
vitronectin
Activin
A
supplementation
pluripotency.
Simplified
will
facilitate
gene
editing
applications
lineage
commitment
studies.
Cell Reports,
Год журнала:
2023,
Номер
42(5), С. 112439 - 112439
Опубликована: Май 1, 2023
Here,
we
report
that
a
chemical
cocktail
(LCDM:
leukemia
inhibitory
factor
[LIF],
CHIR99021,
dimethinedene
maleate
[DiM],
minocycline
hydrochloride),
previously
developed
for
extended
pluripotent
stem
cells
(EPSCs)
in
mice
and
humans,
enables
de
novo
derivation
long-term
culture
of
bovine
trophoblast
(TSCs).
Bovine
TSCs
retain
developmental
potency
to
differentiate
into
mature
exhibit
transcriptomic
epigenetic
(chromatin
accessibility
DNA
methylome)
features
characteristic
trophectoderm
from
early
embryos.
The
established
this
study
will
provide
model
placentation
pregnancy
failure.
International Journal of Molecular Sciences,
Год журнала:
2021,
Номер
22(19), С. 10489 - 10489
Опубликована: Сен. 28, 2021
Pluripotent
stem
cells
(PSCs)
have
been
successfully
developed
in
many
species.
However,
the
establishment
of
bovine-induced
pluripotent
(biPSCs)
has
challenging.
Here
we
report
generation
biPSCs
from
bovine
mesenchymal
(bMSCs)
by
overexpression
lysine-specific
demethylase
4A
(KDM4A)
and
other
reprogramming
factors
OCT4,
SOX2,
KLF4,
cMYC,
LIN28,
NANOG
(KdOSKMLN).
These
exhibited
silenced
transgene
expression
at
passage
10,
had
prolonged
self-renewal
capacity
for
over
70
passages.
The
flat,
primed-like
PSC
colony
morphology
combined
media
knockout
serum
replacement
(KSR)
mTeSR,
but
switched
to
dome-shaped,
naïve-like
mTeSR
medium
2i/LIF
with
single
cell
colonization
capacity.
comparable
proliferation
rate
reported
primed-
or
naïve-state
human
PSCs,
three-germ
layer
differentiation
normal
karyotype.
Transcriptome
analysis
revealed
a
high
similarity
embryonic
(ESCs)
embryos.
can
be
incorporated
into
mouse
embryos,
extended
integration
extra-embryonic
tissues.
Finally,
least
24.5%
cloning
efficiency
could
obtained
nuclear
transfer
(NT)
experiment
using
late
as
donors.
Our
represents
significant
advance
PSCs.
animal,
Год журнала:
2023,
Номер
17, С. 100828 - 100828
Опубликована: Май 1, 2023
The
goal
of
in
vitro
gametogenesis
is
to
reproduce
the
events
sperm
and
oocyte
development
laboratory.
Significant
advances
have
been
made
mouse
last
decade,
but
evolutionary
divergence
from
murine
developmental
program
has
prevented
replication
these
large
mammals.
In
recent
years,
intensive
work
done
humans,
non-human
primates
livestock
elucidate
species-specific
differences
that
regulate
germ
cell
development,
due
number
potential
applications.
One
most
promising
applications
use
gametes
optimize
spread
elite
genetics
cattle.
this
context,
embryonic
stem
cells
posed
as
excellent
candidates
for
platforms.
Here,
we
present
relevant
interest
science,
including
new
types
pluripotent
with
germline
derivation,
characterization
signaling
environment
gonadal
niche,
experimental
systems
used
different
stages
Animals,
Год журнала:
2022,
Номер
12(8), С. 1010 - 1010
Опубликована: Апрель 13, 2022
Early
development
in
mammals
is
characterized
by
the
ability
of
each
cell
to
produce
a
complete
organism
plus
extraembryonic,
or
placental,
cells,
defined
as
pluripotency.
During
subsequent
development,
pluripotency
lost,
and
cells
begin
differentiate
particular
fate.
This
review
summarizes
current
knowledge
features
bovine
embryos
cultured
vitro,
focusing
on
core
genes
(OCT4,
NANOG,
SOX2,
CDX2),
main
chemical
strategies
for
controlling
pluripotent
networks
during
early
development.
Finally,
we
discuss
applicability
manipulating
morula
blastocyst
transition
cattle
species.
Advanced Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 10, 2025
Abstract
Porcine
embryonic
stem
cells
(ESCs)
are
excellent
models
for
exploring
embryogenesis,
producing
genetically
enhanced
farm
animals,
and
improving
breeding.
Various
chemicals
have
been
applied
to
generate
porcine
ESCs
from
embryos,
which
differ
mouse
human
ESC
derivation.
Wnt
inhibitors
XAV939
or
IWR1
required
isolate
maintain
ESCs.
How
specify
fate
decisions
remains
poorly
understood.
Additionally,
whether
can
be
converted
extraembryonic
endoderm
(XEN)
without
genetic
interventions
has
not
reported.
Here,
it
is
reported
that
(i.e.,
IWR1)
safeguard
acquiring
the
XEN
lineage.
rely
on
pluripotency.
Without
them,
exit
pluripotency
convert
cells.
An
efficient
strategy
culture
conditions
further
developed
directly
derive
gene
editing.
The
resulting
exhibit
similar
transcriptome
chromatin
accessibility
features
embryos
contribute
tissues.
This
study
will
deepen
understanding
of
pluripotency,
lay
foundation
deriving
high‐quality
with
germline
chimerism
transmission,
provide
valuable
materials
development
lineage
segregation
in
livestock.
