bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Май 14, 2023
Mitochondria
are
required
for
energy
production
and
even
give
brown
adipose
tissue
(BAT)
its
characteristic
color
due
to
their
high
iron
content
abundance.
The
physiological
function
bioenergetic
capacity
of
mitochondria
connected
the
structure,
folding,
organization
inner-membrane
cristae.
During
aging
process,
mitochondrial
dysfunction
is
observed,
regulatory
balance
dynamics
often
disrupted,
leading
increased
fragmentation
in
cells.
Therefore,
we
hypothesized
that
significant
morphological
changes
BAT
cristae
would
be
present
with
aging.
We
developed
a
quantitative
three-dimensional
(3D)
electron
microscopy
approach
map
network
mouse
test
this
hypothesis.
Using
methodology,
investigated
3D
morphology
adult
(3-month)
aged
(2-year)
murine
via
serial
block
face-scanning
(SBF-SEM)
reconstruction
software
manual
segmentation,
analysis,
quantification.
Upon
investigation,
found
increases
volume,
surface
area,
complexity
decreased
sphericity
BAT,
alongside
decreases
perimeter,
score.
Overall,
these
data
define
nature
structure
across
Biomedicine & Pharmacotherapy,
Год журнала:
2024,
Номер
178, С. 117084 - 117084
Опубликована: Авг. 1, 2024
The
accumulation
of
excess
reactive
oxygen
species
(ROS)
can
lead
to
oxidative
stress
(OS),
which
induce
gene
mutations,
protein
denaturation,
and
lipid
peroxidation
directly
or
indirectly.
expression
is
reduced
ATP
level
in
cells,
increased
cytoplasmic
Ca
Frontiers in Molecular Neuroscience,
Год журнала:
2022,
Номер
14
Опубликована: Янв. 28, 2022
Stroke
is
a
devastating
disease
with
high
mortality
and
disability
rates.
Previous
research
has
established
that
mitochondria,
as
major
regulators,
are
both
influenced
by
stroke,
further
regulated
the
development
of
poststroke
injury.
Mitochondria
involved
in
several
biological
processes
such
energy
generation,
calcium
homeostasis,
immune
response,
apoptosis
regulation,
reactive
oxygen
species
(ROS)
generation.
Meanwhile,
mitochondria
can
evolve
into
various
quality
control
systems,
including
mitochondrial
dynamics
(fission
fusion)
mitophagy,
to
maintain
homeostasis
network.
Various
activities
fission
fusion
associated
integrity
neurological
injury
after
stroke.
Additionally,
proper
mitophagy
seems
be
neuroprotective
for
its
effect
on
eliminating
damaged
while
excessive
disturbs
generation
mitochondria-associated
signal
pathways.
The
balance
between
more
crucial
than
absolute
level
each
process.
A
neurovascular
unit
(NVU)
multidimensional
system
which
cells
release
multiple
mediators
regulate
diverse
signaling
pathways
across
whole
network
way
dynamic
interaction.
turbulence
(MQC)
could
lead
NVU
dysfunctions,
neuron
death,
neuroglial
activation,
blood–brain
barrier
(BBB)
disruption,
neuroinflammation.
However,
exact
changes
effects
MQC
stroke
have
yet
fully
illustrated.
In
this
review,
we
will
discuss
updated
mechanisms
pathophysiology
We
highlight
regulation
potential
therapeutic
target
ischemic
hemorrhagic
European Journal of Cell Biology,
Год журнала:
2023,
Номер
102(4), С. 151371 - 151371
Опубликована: Окт. 31, 2023
The
fluorescence
viewing
of
mitochondria
is
commonly
performed
by
MitoTracker,
a
lipophilic
cationic
dye
that
taken
up
the
mitochondria.
In
this
forum,
we
highlight
several
issues
may
occur
with
including
staining
other
organelles.
Our
aim
to
offer
alternative
dyes
and
discuss
their
advantages
disadvantages.
We
also
options
for
software
alternatives
MitoTracker
expedite
future
experimental
design.
Abstract
Mitochondria
are
required
for
energy
production
and
even
give
brown
adipose
tissue
(BAT)
its
characteristic
color
due
to
their
high
iron
content
abundance.
The
physiological
function
bioenergetic
capacity
of
mitochondria
connected
the
structure,
folding,
organization
inner‐membrane
cristae.
During
aging
process,
mitochondrial
dysfunction
is
observed,
regulatory
balance
dynamics
often
disrupted,
leading
increased
fragmentation
in
cells.
Therefore,
it
hypothesized
that
significant
morphological
changes
BAT
cristae
will
be
present
with
aging.
A
quantitative
3D
electron
microscopy
approach
developed
map
network
mouse
test
this
hypothesis.
Using
methodology,
morphology
investigated
adult
(3‐month)
aged
(2‐year)
murine
via
serial
block
face‐scanning
(SBF‐SEM)
reconstruction
software
manual
segmentation,
analysis,
quantification.
Upon
investigation,
an
increase
found
volume,
surface
area,
complexity
decreased
sphericity
BAT,
alongside
decreases
perimeter,
score.
Overall,
these
data
define
nature
structure
across
The Journal of Cell Biology,
Год журнала:
2023,
Номер
222(10)
Опубликована: Авг. 4, 2023
Mitochondria
are
highly
dynamic
double
membrane–bound
organelles
that
maintain
their
shape
in
part
through
fission
and
fusion.
Mitochondrial
is
performed
by
a
dynamin-related
protein,
Dnm1
(Drp1
humans),
constricts
divides
the
mitochondria
GTP
hydrolysis–dependent
manner.
However,
it
unclear
whether
factors
inside
help
coordinate
process
if
Dnm1/Drp1
activity
sufficient
to
complete
of
both
mitochondrial
membranes.
Here,
we
identify
an
intermembrane
space
protein
required
for
yeast,
which
propose
name
Mdi1
(also
named
Atg44).
Loss
causes
hyperfusion
due
defects
fission,
but
not
lack
recruitment
mitochondria.
conserved
fungal
species,
its
homologs
contain
amphipathic
α-helix,
mutations
disrupt
morphology.
One
model
distorts
membranes
enable
robustly
fission.
Our
work
reveals
cannot
efficiently
divide
without
coordinated
function
Analytical Chemistry,
Год журнала:
2024,
Номер
96(22), С. 9262 - 9269
Опубликована: Май 17, 2024
Dual-organelle
molecular
localizers
represent
powerful
new
tools
allowing
the
exploration
of
interorganelle
physical
contacts
and
subcellular
chemical
communication.
Here,
we
describe
dynamic
probes
to
localize
mitochondria
lipid
droplets
taking
advantage
differential
proton
gradients
present
in
these
organelles
as
well
activity
mitochondrial
esterase.
We
unveil
their
potential
utility
when
organelle
retention
mechanisms
are
synchronized,
an
insight
that
has
not
been
documented
previously.
Our
discoveries
indicate
dual-organelle
serve
a
valuable
multiplexing
assay
during
starvation-induced
autophagy.
The
pioneering
mechanism
they
employ
opens
doors
avoid
using
labile
esters
such
acetoxymethyl
derivatives
which
optimal
imaging
microscopy
assays.
Journal of Fungi,
Год журнала:
2024,
Номер
10(2), С. 115 - 115
Опубликована: Янв. 30, 2024
Cytokinesis
is
required
to
separate
two
daughter
cells
at
the
end
of
mitosis,
and
septins
play
crucial
roles
in
many
aspects
cytokinesis.
While
have
been
intensively
studied
model
organisms,
including
budding
yeast
Saccharomyces
cerevisiae,
relatively
less
characterized
fission
Schizosaccharomyces
pombe,
which
has
proven
be
an
excellent
organism
for
studying
fundamental
cell
biology.
In
this
review,
we
summarize
findings
made
yeasts
mainly
from
four
aspects:
domain
structure
septins,
localization
during
cycle,
regulating
cytokinesis,
regulatory
proteins
septins.
Redox Biology,
Год журнала:
2024,
Номер
73, С. 103174 - 103174
Опубликована: Апрель 29, 2024
Ribosomes
mediate
protein
synthesis,
which
is
one
of
the
most
energy-demanding
activities
within
cell,
and
mitochondria
are
main
sources
generating
energy.
How
mitochondrial
morphology
functions
adjusted
to
cope
with
ribosomal
defects,
can
impair
synthesis
affect
cell
viability,
poorly
understood.
Here,
we
used
fission
yeast
Schizosaccharomyces
Pombe
as
a
model
organism
investigate
interplay
between
ribosome
mitochondria.
We
found
that
insult,
caused
by
absence
Rpl2702,
activates
signaling
pathway
involving
Sty1/MAPK
mTOR
modulate
functions.
Specifically,
demonstrated
induces
fragmentation
in
mTOR-independent
manner
while
both
increases
levels
membrane
potential
reactive
oxygen
species
(mROS).
Moreover,
acts
upstream
Tor1/TORC2
required
activate
Tor2/TORC1.
The
enhancements
mROS
function
promote
proliferation
cells
bearing
defects.
Hence,
our
study
reveals
previously
uncharacterized
Sty1/MAPK-mTOR
axis
regulates
response
insults
provides
new
insights
into
molecular
physiological
adaptations
impaired
synthesis.
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(5), С. 2152 - 2152
Опубликована: Фев. 27, 2025
Mitochondria
are
involved
in
a
wide
array
of
critical
cellular
processes
from
energy
production
to
cell
death.
The
morphology
(size
and
shape)
mitochondrial
compartments
is
highly
responsive
both
intracellular
extracellular
conditions,
making
these
organelles
dynamic.
Nutrient
levels
stressors
inside
outside
the
inform
balance
fission
fusion
recycling
components
known
as
mitophagy.
study
its
implications
human
disease
microbial
engineering
have
gained
significant
attention
over
past
decade.
yeast
Saccharomyces
cerevisiae
offers
valuable
model
system
for
studying
mitochondria
due
ability
survive
without
respiring,
genetic
tractability,
high
degree
similarity
across
eukaryotic
species.
Here,
we
review
how
interplay
between
fission,
fusion,
biogenesis,
mitophagy
regulates
dynamic
nature
networks
mammalian
systems
with
an
emphasis
on
organism.
Additionally,
examine
crucial
role
inter-organelle
interactions,
particularly
endoplasmic
reticulum,
regulating
dynamics.
dysregulation
any
gives
rise
abnormal
morphologies,
which
serve
distinguishing
features
numerous
diseases,
including
Parkinson's
disease,
Alzheimer's
cancer.
Notably,
models
contributed
revealing
underlying
mechanisms
driving
states.
In
addition
furthering
our
understanding
pathologic
processes,
aberrant
morphologies
increasing
interest
seemingly
distant
field
metabolic
engineering,
following
discovery
that
compartmentalization
certain
biosynthetic
pathways
within
can
significantly
improve
chemical
production.
this
review,
utility
organism
healthy
states,
explore
nascent
discuss
methods
available
quantification
classification
key
morphologies.
