Expression profiling of juvenile hormone and ecdysteroid biosynthesis genes during the development and reproduction of Phytoseiulus persimilis DOI

Lina Fan,

Wang Endong,

Bo Zhang

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Июль 3, 2024

Abstract Juvenile hormone (JH) and ecdysteroids play a vital role in arthropods growth development. Significant progress has been made recent years regarding the roles molecular mechanisms of juvenile hormones molting steroids regulating insect metamorphosis developmental. However, related genes regulatory Acari remain unclear. In this study, we conducted comprehensive analysis by screening genomic transcriptomic data to identify three associated with biosynthesis five ecdysteroid predatory mite, Phytoseiulus persimilis. We analyzed spatial-temporal expression patterns each gene at different developmental stages Real-time quantitative PCR (qRT-PCR). The levels signaling factors PpJHAMT PpKr-h1 PpSad PpShd were highest during larval stage. addition, Halloween PpSpo exhibited serrated pattern immature stages. Similarly, PpDib was expressed throughout developemental stage, early always higher than late one. ecdysone receptor PpEcR found be consistently low after fertilization female adults, increased significantly, reaching level approximately 4.28 times average level. Upon verification function through RNAi (RNA interference), it observed that total egg production pre-mating post-mating adults treated dsKr-h1 (double-stranded RNA targeting Kr-h1) decrease 8.73% 13.30%, respectively. case nymphs dsEcR EcR), failure observed, accompanied severe dorsum crumpling death. functional two biosynthesis-related P. persimilis offer preliminary understanding development mites.

Язык: Английский

Regulatory Mechanisms of Vitellogenesis in Insects DOI Creative Commons
Zhongxia Wu,

Libin Yang,

Qiongjie He

и другие.

Frontiers in Cell and Developmental Biology, Год журнала: 2021, Номер 8

Опубликована: Янв. 28, 2021

Vitellogenesis is pre-requisite to insect egg production and embryonic development after oviposition. During vitellogenesis, the yolk protein precursor vitellogenin (Vg) mainly synthesized in fat body, transported by hemolymph through intercellular spaces (known as patency) follicular epithelium reach membrane of maturing oocytes, sequestered into oocytes via receptor-mediated endocytosis. Insect vitellogenesis governed two critical hormones, sesquiterpenoid juvenile hormone (JH) ecdysteriod 20-hydroxyecdysone (20E). JH acts principal gonadotropic stimulate basal hemimetabolous most holometabolous insects. 20E for some hymenopterans, lepidopterans dipterans. Furthermore, microRNA (miRNA) nutritional (amino acid/Target Rapamycin insulin) pathways interplay with signaling cascades control vitellogenesis. Revealing regulatory mechanisms underlying understanding reproduction helpful developing new strategies pest control. Here, we outline recent research progress molecular action along role miRNA sensor regulating We highlight advancements coordination hormone, pathways.

Язык: Английский

Процитировано

176
Post-transcriptional regulation of insect metamorphosis and oogenesis DOI
Jiasheng Song, Shutang Zhou

Cellular and Molecular Life Sciences, Год журнала: 2019, Номер 77(10), С. 1893 - 1909

Опубликована: Ноя. 13, 2019

Язык: Английский

Процитировано

92
Hairy and Krüppel homolog 1 Comediate the Action of Juvenile Hormone/Methoprene-Tolerant Signaling Pathway in Vitellogenesis of Spodoptera frugiperda (J.E. Smith) DOI

Shipeng Han,

Xiao-Qi Wang,

Hui Han

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Янв. 2, 2025

Spodoptera frugiperda is a major migratory invasive pest and of global concern. Vitellogenesis, crucial process for population multiplication in oviparous insects, regulated by endocrine hormones. In this study, three primary responders to JH signaling, the receptor gene Met, downstream transcription factor Kr-h1 Hairy, were first cloned identified. RNA interference results showed that silencing SfMet significantly down-regulated levels SfKr-h1 SfHairy, as well key reproductive genes Vitellogenin (SfVg) (SfVgR). Similarly, SfHairy also inhibited SfVg SfVgR. Silencing SfMet, SfKr-h1, resulted blocked ovarian development significant decrease reproduction. These findings confirm Hairy comediate action JH-Met signaling pathway vitellogenesis S. frugiperda, providing new targets insights control.

Язык: Английский

Процитировано

1
Kr-h1, a Cornerstone Gene in Insect Life History DOI Creative Commons
Qianyu He, Yuanxi Zhang

Frontiers in Physiology, Год журнала: 2022, Номер 13

Опубликована: Апрель 27, 2022

Insect life cycle is coordinated by hormones and their downstream effectors. Krüppel homolog1 (Kr-h1) one of the crucial effectors which mediates actions two critical insects, juvenile hormone (JH) 20-hydroxyecdysone (20E). It a transcription factor with DNA-binding motif eight C 2 H zinc fingers found to be conserved among insect orders. The expression Kr-h1 fluctuant during development high abundance in instars lower levels final instar pupal stage, reappearance adults, governed coordination JH, 20E, miRNAs. dynamic pattern closely linked its function entire insects. Over past several years, accumulating studies have advanced our understanding role development. acts as universal antimetamorphic both hemimetabolous holometabolous species directly inhibiting 20E signaling genes Broad-Complex ( Br-C ) Ecdysone induced protein 93F E93 ), steroidogenic enzyme involved ecdysone biosynthesis. Meanwhile, it promotes vitellogenesis ovarian majority studied In addition, regulates behavioral plasticity caste identity, neuronal morphogenesis, maturation sexual behavior, well embryogenesis metabolic homeostasis. Hence, cornerstone regulator life.

Язык: Английский

Процитировано

36
Krüppel-homolog 1 exerts anti-metamorphic and vitellogenic functions in insects via phosphorylation-mediated recruitment of specific cofactors DOI Creative Commons
Zhongxia Wu,

Libin Yang,

Huihui Li

и другие.

BMC Biology, Год журнала: 2021, Номер 19(1)

Опубликована: Окт. 8, 2021

Abstract Background The zinc-finger transcription factor Krüppel-homolog 1 (Kr-h1) exerts a dual regulatory role during insect development by preventing precocious larval/nymphal metamorphosis and in stimulating aspects of adult reproduction such as vitellogenesis. However, how Kr-h1 functions both transcriptional repressor juvenile an activator remains elusive. Here, we use the Locusta migratoria to dissect molecular mechanism which at these distinct developmental stages. Results We report that kinase PKCα triggers phosphorylation amino acid residue Ser 154 , step essential for its functions. During stage, phosphorylated recruits corepressor, C-terminal binding protein (CtBP). complex CtBP represses Ecdysone induced 93F ( E93 ) consequently prevents juvenile-to-adult transition. In insects, coactivator, CREB-binding (CBP), promotes vitellogenesis inducing expression Ribosomal L36 . Furthermore, with concomitant inhibition is evolutionarily conserved across orders. Conclusion Our results suggest indispensable recruitment cofactors, anti-metamorphic vitellogenic actions insects. data shed new light on understanding regulation function JH-regulated reproduction.

Язык: Английский

Процитировано

27
siRNA self-assembled carboxymethyl chitosan and pymetrozine nucleic pesticides for enhanced control of Sogatella furcifera: Bidirectional transduction and detoxification inhibition DOI
Changwei Gong, Wei Wang,

Yanxin Ma

и другие.

Carbohydrate Polymers, Год журнала: 2025, Номер 354, С. 123328 - 123328

Опубликована: Янв. 28, 2025

Язык: Английский

Процитировано

0
RNA interference knockdown of the nuclear receptor HR3 suppresses vitellogenesis in Chilo suppressalis DOI

J S Hu,

Qingzhen He,

Yang Cui

и другие.

Molecular Biology Reports, Год журнала: 2025, Номер 52(1)

Опубликована: Май 21, 2025

Язык: Английский

Процитировано

0
Krüppel homolog 1 regulates photoperiodic reproductive plasticity in the cabbage beetle Colaphellus bowringi DOI
Shuang Guo, Qingwen Wu, Zhong Tian

и другие.

Insect Biochemistry and Molecular Biology, Год журнала: 2021, Номер 134, С. 103582 - 103582

Опубликована: Апрель 24, 2021

Язык: Английский

Процитировано

21
FAR knockout significantly inhibits Chilo suppressalis survival and transgene expression of double‐stranded FAR in rice exhibits strong pest resistance DOI Creative Commons

Yingjuan Sun,

Youwei Gong,

Qingzhen He

и другие.

Plant Biotechnology Journal, Год журнала: 2022, Номер 20(12), С. 2272 - 2283

Опубликована: Авг. 9, 2022

Chilo suppressalis is one of the most prevalent and damaging rice pests, causing significant economic losses each year. Chemical control currently primary method controlling C. suppressalis. However, indiscriminate use chemical insecticides increases pest resistance, pollutes environment poses a health threat to humans livestock, highlighting need find safer, more pest-specific effective alternatives control. Plant-mediated RNA interference (RNAi) promising agricultural that highly has less an impact on environment. Using multi-sgRNAs/Cas9 technology delete Fatty acyl-CoA reductase (FAR) in G0 generation, we show downregulating FAR transcription may significantly increase mortality rate darken epidermis compared with Subsequently, developed dsFAR transgenic lines using Agrobacterium-mediated genetic transformation then screened three strains expressing at high levels transcriptional level analysis. stems, laboratory feeding bioassay indicated least line (L#10) displayed particularly insect than 80%. In field trials, resistance damage. Collectively, these results suggest potential new environment-friendly, species-specific strategy for management.

Язык: Английский

Процитировано

15
Impact of JH Signaling on Reproductive Physiology of the Classical Insect Model, Rhodnius prolixus DOI Open Access
Jimena Leyria, Ian Orchard, Angela B. Lange

и другие.

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(22), С. 13832 - 13832

Опубликована: Ноя. 10, 2022

In adult females of several insect species, juvenile hormones (JHs) act as gonadotrophic hormones, regulating egg production. JH binds to its nuclear receptor, Methoprene tolerant (Met), triggering dimerization with the protein Taiman (Tai). The resulting active complex induces transcription response genes, such Krüppel homolog 1 (Kr-h1). this study we report for first time participation isoform III skipped bisepoxide (JHSB3) and signaling pathway in reproductive fitness classical model Rhodnius prolixus. topical application synthetic JHSB3 increases transcript expression yolk precursors (YPPs), mainly by fat body but also ovaries, second source YPPs. These results are confirmed ex vivo assays. contrast, when cascade is impaired via RNA interference downregulating RhoprMet RhoprTai mRNA, production inhibited. Although RhoprKr-h1 highly dependent on signaling, it not involved rather successful hatching. This research contributes missing pieces action which was postulated almost 100 years ago.

Язык: Английский

Процитировано

15