Molecular ecology and microbiomes in the wild: methodological advances, common pitfalls and future directions DOI Open Access

Nick Fountain‐Jones,

Tatiana Giraud, Lucie Zinger

и другие.

Authorea (Authorea), Год журнала: 2023, Номер unknown

Опубликована: Сен. 27, 2023

The study of microbiomes across organisms and environments has become a prominent focus in molecular ecology. This perspective article explores methodological advancements, common challenges future directions the field. Key research areas include understanding drivers microbiome community assembly, linking composition to host genetics, exploring microbial functions, transience, spatial partitioning, disentangling non-bacterial components microbiome. Methodological such as quantifying absolute abundances, sequencing complete genomes, utilizing novel statistical approaches, are also useful tools for complex diversity patterns. Our aims encourage robust practices studies inspire researchers explore next frontier this rapidly changing

Язык: Английский

Molecular ecology of microbiomes in the wild: Common pitfalls, methodological advances and future directions DOI Open Access
Nicholas M. Fountain‐Jones, Tatiana Giraud, Lucie Zinger

и другие.

Molecular Ecology, Год журнала: 2023, Номер 33(2)

Опубликована: Ноя. 28, 2023

Abstract The study of microbiomes across organisms and environments has become a prominent focus in molecular ecology. This perspective article explores common challenges, methodological advancements, future directions the field. Key research areas include understanding drivers microbiome community assembly, linking composition to host genetics, exploring microbial functions, transience spatial partitioning, disentangling non‐bacterial components microbiome. Methodological such as quantifying absolute abundances, sequencing complete genomes, utilizing novel statistical approaches, are also useful tools for complex diversity patterns. Our aims encourage robust practices studies inspire researchers explore next frontier this rapidly changing

Язык: Английский

Процитировано

11

Another tool in the toolbox: Aphid‐specific Wolbachia protect against fungal pathogens DOI Creative Commons
Clesson H. V. Higashi,

Vilas Patel,

Bryan Kamalaker

и другие.

Environmental Microbiology, Год журнала: 2024, Номер 26(11)

Опубликована: Ноя. 1, 2024

Abstract Aphids harbor nine common facultative symbionts, most mediating one or more ecological interactions. Wolbachia pipientis , well‐studied in other arthropods, remains poorly characterized aphids. In Pentalonia nigronervosa and P. caladii global pests of banana, was initially hypothesized to function as a co‐obligate nutritional symbiont alongside the traditional obligate Buchnera . However, genomic analyses failed support this role. Our sampling across numerous populations revealed that than 80% aphids carried an M‐supergroup strain ( wPni ). The lack fixation further supports status for while high infection frequencies these entirely asexual strongly suggest confers net fitness benefits. Finding no correlation between presence food plant use, we challenged ‐infected with natural enemies. Bioassays conferred significant protection against specialized fungal pathogen Pandora neoaphidis ) but not generalist pathogens parasitoids. also improved aphid absence enemy challenge. Thus, identified first clear benefits aphid‐associated strains specifically. Aphid‐ systems provide unique opportunities merge key models symbiosis better understand dynamics mechanisms underpinning symbiont‐mediated phenotypes.

Язык: Английский

Процитировано

4

Mobile elements create strain‐level variation in the services conferred by an aphid symbiont DOI Creative Commons

Vilas Patel,

Nicole Lynn‐bell,

Germain Chevignon

и другие.

Environmental Microbiology, Год журнала: 2023, Номер 25(12), С. 3333 - 3348

Опубликована: Окт. 20, 2023

Heritable, facultative symbionts are common in arthropods, often functioning host defence. Despite moderately reduced genomes, retain evolutionary potential through mobile genetic elements (MGEs). MGEs form the primary basis of strain-level variation genome content and architecture, correlate with variability symbiont-mediated phenotypes. In pea aphids (Acyrthosiphon pisum), type toxin-encoding bacteriophages (APSEs) carried by bacterium Hamiltonella defensa correlates strength defence against parasitoids. However, co-inheritance creates difficulties for partitioning their relative contributions to aphid Here we identified isolates H. that were nearly identical except APSE type. When holding genotype constant, protection levels corresponded virulence module Results further indicated APSEs move repeatedly within some clades providing a mechanism rapid evolution anti-parasitoid defences. Strain also presence second symbiont Fukatsuia symbiotica. Predictions nutritional interactions structured this coinfection not supported comparative genomics, but bacteriocin-containing plasmids unique co-infecting strains may contribute pairing. conclusion, strain diversity, joint capacities horizontal transfer symbionts, emergent players arthropods.

Язык: Английский

Процитировано

7

The nutritional dimension of facultative bacterial symbiosis in aphids: current status and methodological considerations for future research DOI Creative Commons
François Renoz

Current Research in Insect Science, Год журнала: 2023, Номер 5, С. 100070 - 100070

Опубликована: Дек. 20, 2023

Aphids are valuable models for studying the functional diversity of bacterial symbiosis in insects. In addition to their ancestral obligate nutritional symbiont

Язык: Английский

Процитировано

6

Competition among host‐specific lineages of Poecilochirus carabi mites influences the extent of co‐adaptation with their Nicrophorus vespilloides burying beetle hosts DOI Creative Commons
Syuan‐Jyun Sun, Rebecca M. Kilner

Ecology and Evolution, Год журнала: 2024, Номер 14(1)

Опубликована: Янв. 1, 2024

Reciprocal selection between symbiotic organisms and their hosts can generate variations in local adaptation them. Symbionts often form species complexes with lineages partially adapted to various hosts. However, it is unclear how interactions among these influences geographic variation the extent of host-symbiont adaptation. We addressed this shortcoming experiments on burying beetles

Язык: Английский

Процитировано

2

Clines of resistance to parasitoids: the multifarious effects of temperature on defensive symbioses in insects DOI Creative Commons
Cameron M. Hudson,

Dominic Stalder,

Christoph Vorburger

и другие.

Current Opinion in Insect Science, Год журнала: 2024, Номер 64, С. 101208 - 101208

Опубликована: Май 29, 2024

Insects are frequently infected with heritable bacterial endosymbionts. Some of them confer resistance to parasitoids. Such defensive symbionts sensitive variation in temperature. Drawing predominantly from the literature on aphids and flies, we show that temperature can affect reliability maternal transmission strength protection provided by symbionts. Costs infection also be temperature-dependent may even turn into benefits under extreme temperatures, e.g. when increase heat tolerance. Alone or combination, these mechanisms drive temperature-associated (latitudinal) clines prevalence This has important consequences for host-parasitoid coevolution, as relative importance host-encoded vs. symbiont-provided defenses will shift along such clines.

Язык: Английский

Процитировано

2

Cryptic host specialisation within Poecilochirus carabi mites explains population differences in the extent of co-adaptation with their burying beetle Nicrophorus vespilloides hosts DOI Creative Commons
Syuan‐Jyun Sun, Rebecca M. Kilner

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2019, Номер unknown

Опубликована: Май 17, 2019

Abstract Reciprocal selection between symbiotic organisms and their hosts can generate variations in local adaptation them. Symbionts often form species complexes with lineages partially adapted to various hosts. However, it is unclear how interactions among these influences geographic variation host-symbiont adaptation. We addressed this shortcoming experiments on burying beetles Nicrophorus vespilloides specialist phoretic mite Poecilochirus carabi two adjacent woodlands. Burying transport mites vertebrate carrion upon which they both reproduce. P. appears be a complex, distinct that specialise breeding alongside different species. found one wood (Gamlingay Woods), N. carries mixture of lineages, each lineage corresponding the four inhabits wood. beetle coexist neighbouring Waresley Woods here predominantly favours . Mite mixing alters degree for mites, affecting reproductive success variably across In Gamlingay, reduced success, while experimentally purifying enhanced it. The near pure negligibly impacted varied host specificity: Gamlingay had greatest beetles, performed less well beetles. By contrast, consistent regardless beetle’s woodland origin. conclude there some evidence its specific have co-adapted. neither nor breed other lineages. This, we suggest, causes Gaminglay extent mites.

Язык: Английский

Процитировано

3

Modelagem de interações inseto simbionte: efeito do controle químico na distribuição de simbiontes DOI Creative Commons

Lucas Santos Canuto

Опубликована: Фев. 16, 2024

Modeling inset-symbiont interactions: the effect of chemical control in symbiont distributionInsect-symbiont interactions have been getting a lot attention literature because their interactive nature, mostly, those positive result for both parties.These can lead to interesting case studies agriculture, benefits from symbiosis.Harboring some symbionts different changes host, behavior, physiology, biology and ecology.In particular, resistance or protection host insect under scenarios selective pressures insecticides parasitoids, perspective where an individual is not just another member species.In that case, organism seen as holobiont being, integrate own microbiome community part individual.In this research, we propose computational individual-based model, with Monte Carlo simulations, test hypothesis pressure caused by presence helps selecting increasing number hosts detoxification qualities related insecticide.The model developed allowed use parameters present literature, testing out described using study on Spodoptera frugiperda, has Leclercia adecarboxylata Pseudomonas psychrotolerans, each one capable degrading compounds Chlorpyrifos ethyl Spinosad respectively.Using simulations made it was found L. had expected dynamic according presented, there growth symbiont's population when they bring benefits, lower stable cases wasn't much benefit, other hand, P. psychrotolerans didn't significative any made, probably its high constitutive cost host.Another more populations change time population, statistics tests made.It's possible say developed, while flaws, room improvement questions yet be explored, able help answering main raised research.

Язык: Английский

Процитировано

0

Molecular ecology and microbiomes in the wild: methodological advances, common pitfalls and future directions DOI Open Access

Nick Fountain‐Jones,

Tatiana Giraud, Lucie Zinger

и другие.

Authorea (Authorea), Год журнала: 2023, Номер unknown

Опубликована: Сен. 27, 2023

The study of microbiomes across organisms and environments has become a prominent focus in molecular ecology. This perspective article explores methodological advancements, common challenges future directions the field. Key research areas include understanding drivers microbiome community assembly, linking composition to host genetics, exploring microbial functions, transience, spatial partitioning, disentangling non-bacterial components microbiome. Methodological such as quantifying absolute abundances, sequencing complete genomes, utilizing novel statistical approaches, are also useful tools for complex diversity patterns. Our aims encourage robust practices studies inspire researchers explore next frontier this rapidly changing

Язык: Английский

Процитировано

0