Simultaneous silencing of gut nucleases and a vital target gene by adult dsRNA feeding enhances RNAi efficiency and mortality inCeratitis capitataadults DOI Creative Commons
Gennaro Volpe, Sarah Maria Mazzucchiello,

Noemi Rosati

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Авг. 5, 2024

Abstract Ceratitis capitata , known as Mediterranean fruit fly (Medfly), is a major dipteran pest significantly impacting and vegetable farming. Currently, its control heavily relies mainly on chemical insecticides, which pose health risks have effects pollinators. A friendly species-specific alternative strategy involves providing double-stranded RNA (dsRNA) through feeding to disrupt essential functions in insects, poorly explored species. Previous reports Orthoptera Coleoptera species suggested that dsRNA degradation by two specific nucleases the intestinal lumen among obstacle feeding-mediated RNAi insects. In our study, we experimented with three-day adult using combination of molecules target expression ATPase vital gene nucleases. These were recently tested separately Tephritidae species, showing limited effectiveness [1,2]. contrast, observed 79% mortality over seven days, was associated decrease mRNA levels three targeted genes. As expected, also reduction following against This research illustrates potential utilizing pesticides achieve rates Medfly adults targeting crucial genes Furthermore, it underscores importance exploring RNAi-based approaches for management Simple Summary The insect belonging Orthoptera, Hemiptera, can be based novel emerging pesticides. consist delivered larvae or adults, suppress RNA-RNA sequence complementarity interference. However, fewer studies been performed Two shown suppressing enzymes degrading external improve rates. (Tephritidae), beneficial attempts induce dsRNA-feeding this other such Bactrocera tryoni B. dorsalis showed some effectiveness, but often limited. We improved method simultaneously silencing gene. found mix dsRNAs able much higher (79%) within one week, only days feeding.

Язык: Английский

Next Generation dsRNA-Based Insect Control: Success So Far and Challenges DOI Creative Commons
Rahul Nitnavare, Joorie Bhattacharya, Satnam Singh

и другие.

Frontiers in Plant Science, Год журнала: 2021, Номер 12

Опубликована: Окт. 18, 2021

RNA interference (RNAi) is a method of gene silencing where dsRNA digested into small interfering (siRNA) in the presence enzymes. These siRNAs then target homologous mRNA sequences aided by RNA-induced complex (RISC). The mechanism uptake has been well studied and established across many living organisms including insects. In insects, RNAi novel potential tool to develop future pest management means targeting various classes insects dipterans, coleopterans, hemipterans, lepidopterans, hymenopterans isopterans. However, extent individual class varies due underlying mechanisms. present review focuses on three major insect viz lepidopterans coleopterans rationale behind this lies fact that studies pertaining extensively performed these groups. Additionally, harbour agriculturally important species which require attention. Interestingly, all exhibit varying levels efficiencies with exhibiting maximum response, while hemipterans are relatively inefficient. Lepidopterans other hand, show minimum response RNAi. This attributed facts few being endosomal escape, high activity dsRNA-specific nucleases, highly alkaline gut environment renders unstable. Various methods have ensure safe delivery biological system insect. most common for administration supplementing diet via spraying onto leaves commonly eaten parts plant. environment-friendly superior hazardous effects pesticides. Another involves submergence root systems solutions subsequent phloem. more recent techniques nanoparticle- Agrobacterium -mediated systems. novelty biotechnological recalcitrant nature certain crops, further optimization required. emphasizes developments hurdles efficient also discusses detail development new enhance efficiency using liposomes nanoparticles, transplastomics, microbial-mediated chemical methods.

Язык: Английский

Процитировано

52
CRISPR‐mediated mutagenesis of the odorant receptor co‐receptor (Orco) gene disrupts olfaction‐mediated behaviors in Bactrocera dorsalis DOI
Li Xu, Hong‐Bo Jiang,

Kai‐Yue Tang

и другие.

Insect Science, Год журнала: 2022, Номер 29(5), С. 1275 - 1286

Опубликована: Янв. 5, 2022

Olfaction plays an essential role in insect behavior such as host location, foraging, mating, and oviposition. The odorant receptor co-receptor (Orco) is obligatory indispensable odor perception. Here, we characterized the Orco gene from oriental fruit fly, Bactrocera dorsalis (Hendel), a notorious agriculture pest. olfactory deficiency mutants were generated by editing BdorOrco using CRISPR/Cas9 system. Electroantennograms (EAG) preference assays confirmed that BdorOrco-/- mutant flies had reduced perception of methyl eugenol, β-caryophyllene, ethyl acetate. Oviposition bioassays showed eggs laid females mediated benzothiazole 1-octen-3-ol significantly decreased. In addition, took longer time to locate food source compared with wild type (WT) flies. Altogether, our data indicated for multiple physiological processes B. dorsalis, it expands understanding function Orco.

Язык: Английский

Процитировано

26
Control of two insect pests by expression of a mismatch corrected double‐stranded RNA in plants DOI Creative Commons
Yi Dong, Qi Zhang,

Yarou Mao

и другие.

Plant Biotechnology Journal, Год журнала: 2024, Номер 22(7), С. 2010 - 2019

Опубликована: Март 1, 2024

Summary RNA interference (RNAi) has emerged as an efficient technology for pest control by silencing the essential genes of targeted insects. Owing to its nucleotide sequence‐guided working mechanism, RNAi a high degree species‐specificity without impacts on non‐target organisms. However, plants are inevitably under threat two or more insect pests in nature, species‐specific mode RNAi‐based restricts wide application control. In this study, we artificially designed intermediate dsRNA ( iACT ) targeting β‐Actin ACT sap‐sucking Bemisia tabaci and Myzus persicae mutual correction their mismatches. When expressing hairpin (hp from tobacco nuclear genome, transgenic well protected both B. M. , either individually simultaneously, evidenced reduced fecundity suppressed gene expression, whereas expression hpRNA BtACT MpACT could only confer specific resistance respectively. sum, our data provide novel proof‐of‐concept that different species be simultaneously controlled artificial synthesis with sequence optimization, which expands range methods crop protection.

Язык: Английский

Процитировано

5
RNA interference (RNAi) applications to the management of fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae): Its current trends and future prospects DOI Creative Commons

Megersa Kebede,

Tarekegn Fite

Frontiers in Molecular Biosciences, Год журнала: 2022, Номер 9

Опубликована: Сен. 7, 2022

The fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae) is among the invasive insect pests that damages maize and sorghum, high-priority crops in newly colonized agro-ecologies, including African contexts. Owing to increasing infestation of pest limitations current conventional methods for its management, there a call discovering advanced management approaches. RNA interference (RNAi) an emerging molecular tool showing flexible potential S. . We conducted search recent application RNAi literature using Google Scholar Mendeley find papers on tools led growth inhibition, developmental aberrations, reduced fecundity, mortality, mainly by disruption normal biological processes pest. Although efforts have been made accelerate utility RNAi, many factors limit efficiency achieve successful control over RNAi’s bioactivity economic ecological acceptability, continued research should focus improving broad applicability, field conditions. Screening identification key target genes be priority task effective sustainable this via RNAi. In addition, clear understanding present status utilization paramount importance improve efficiency. Therefore, review, we highlight biology mechanism as foundation Then, discuss knowledge approach affecting application. Finally, prospects RNAi-based are highlighted future frugiperda.

Язык: Английский

Процитировано

19
Postcopulatory Behavior of Tephritid Flies DOI Creative Commons
Diana Pérez‐Staples, Solana Abraham

Annual Review of Entomology, Год журнала: 2022, Номер 68(1), С. 89 - 108

Опубликована: Окт. 6, 2022

Mating produces profound changes in the behavior of female flies, such as an increase oviposition, reduction sexual receptivity, feeding, and even excretion. Many these are produced by copulation, sperm, accessory gland products that males transfer to females during mating. Our knowledge on function male ejaculate its effect insects is still incipient. In this article, we review peri- postcopulatory behaviors tephritid flies. We address effects copulatory behavior; copula duration; ejaculate, remating behavior. species from families pests economic importance; thus, understanding mating contributes both developing more effective environmentally friendly control methods furthering our evolutionary implications intersexual competition conflict.

Язык: Английский

Процитировано

19
Olfactory receptors in Bactrocera species for sustainable fruit fly management: A review and future perspectives DOI Creative Commons
Saleem Jaffar, Guy Smagghe, Yongyue Lu

и другие.

Physiological Entomology, Год журнала: 2024, Номер 49(2), С. 67 - 90

Опубликована: Янв. 24, 2024

Abstract Molecular studies on odorant receptors (ORs), odorant‐binding proteins (OBPs) and the functioning of receptor pheromone signal transduction in fruit fly Bactrocera species have expanded exponentially during past few decades. OBPs contribute to sensing olfactory system (OS) via odorants through sensillum lymph. However, ORs, a family G‐protein‐coupled various other species, exhibit heightened responsiveness multiple chemical odours such as hormones, sensory stimuli neurotransmitters. The apparent mechanism involves combinatorial code encompassing both peripheral antennal lobe processing, facilitating reception sexual pheromones environmental cues. OS is specifically designed recognize process information from volatile signals, these signals play an important function flies. Insects rely chemicals navigate comprehend their surroundings. A mature insect composed two pairs sensillae‐covered palps, antennae primary appendages anterior head. It has been shown that chemosensory gene families odour perception. These include neuroreceptor families, OBPs, neuron membrane proteins. Additionally, there are three divergent chemoreceptors, namely ionotropic gustatory receptors. Methods based systematic biology, molecular biology bioinformatics tools rapidly emerged investigate communication systems provide new insights for management many agricultural pest. Several aromatic compounds, including semiochemicals pheromones, employed defend crops animals destructive flies invasive frugivorous species. To promote expansion cropping system, utilization phytochemical lures can be convenient sustainable agriculture production enhance food security. Hence, this review examined state art insects with focus pest identify semiochemical receptors, protein (CSRs), well practical applications biological control integrated highlighted.

Язык: Английский

Процитировано

4
Geographical distribution of two major quarantine fruit flies (Bactrocera minax Enderlein and Bactrocera dorsalis Hendel) in Sichuan Basin based on four SDMs DOI Creative Commons
Yanli Xia, Jinpeng Zhao,

Jian Ding

и другие.

PeerJ, Год журнала: 2024, Номер 12, С. e16745 - e16745

Опубликована: Янв. 8, 2024

Both

Язык: Английский

Процитировано

3
Chitosan double‐stranded RNA nanocomplexes for Piezodorus guildinii control DOI Open Access
Claudia Schvartzman, S. Murchio, Analía Castro

и другие.

Pest Management Science, Год журнала: 2025, Номер unknown

Опубликована: Март 5, 2025

Piezodorus guildinii (Westwood, Hemiptera: Pentatomidae) causes economic losses in soybean crops by diminishing productivity, impacting grain size, oil content, and germination power. RNA interference (RNAi), a conserved gene regulation process initiated double-stranded (dsRNA), may be used as safe technique integrated pest management strategies. Conjugating dsRNA with cationic polymers, which protect from degradation has shown to enhance this response. This study aims assess the impact of administration on viability P. evaluate dsRNA-chitosan nanocomplexes control strategy. Ten target genes were selected, dsRNAs designed synthesized. In injection assays, significant mortality exceeding 76% was observed, while ingestion also showed differences, reaching 49%. The targeting Srp54k selected chitosan-dsRNA nanocomplex These demonstrated enhanced stability under RNase treatment vitro, and, vivo cumulative corrected rate 66% reported. Consistent these findings, RT-qPCR analysis revealed that expression levels treated insects significantly reduced compared at 24, 48, 72 h post for targeted genes. work, we evaluated effect determined its molecular silencing effects. Additionally, synthesized characterized nanoparticles, improved efficacy terms stability. findings are promising development new strategies stink bugs control. © 2025 Society Chemical Industry.

Язык: Английский

Процитировано

0
Identification of RNAi efficiency‐related gene in the green peach aphid, Myzus persicae (Hemiptera), based on comparative transcriptomics DOI

Xiu‐Cheng Xie,

Feng Shang, Yujing Liu

и другие.

Pest Management Science, Год журнала: 2025, Номер unknown

Опубликована: Апрель 9, 2025

Abstract BACKGROUND RNA interference (RNAi) is a promising strategy for aphid control; however, its practical application has been limited by low efficiency in aphids. To address this, we combined transcriptomic analysis with an ‘RNAi of RNAi’ approach to identify genes associated RNAi green peach Myzus persicae . RESULTS Using the artificial diet‐mediated dsRNA delivery method, observed that ingestion both dsMpHunchback and dsfusion (a targeting three genes) induced effects. These included significant gene silencing reduced nymph production at 24, 36, 48 h post‐feeding compared dsGFP. Expression profiling core machinery revealed samples from 36 were critical efficiency, prompting their selection transcriptome sequencing. Weighted co‐expression network (WGCNA) Differentially Expressed Genes (DEGs) employed elucidate RNA‐seq data. Subsequent validation through assay demonstrated functional roles encoding basic region leucine zipper ( gene11325 ), cathepsin B‐like gene12476 glucosidase KIAA1161‐like gene6489 piggyBac transposable element‐derived protein 3‐like gene7736 ). Notably, whereas g ene12476 appeared exert compensatory effects counteracted dsfusion. CONCLUSION findings highlight diversity influencing aphid, enhancing our understanding mechanisms establishing foundation optimizing RNAi‐based control strategies. © 2025 Society Chemical Industry.

Язык: Английский

Процитировано

0
Transcription and functional analysis of phosphine fumigation regulating heat responses in Bactrocera dorsalis DOI

Yisha Ma,

Li Li,

Hang Zou

и другие.

Pesticide Biochemistry and Physiology, Год журнала: 2025, Номер 212, С. 106433 - 106433

Опубликована: Апрель 29, 2025

Язык: Английский

Процитировано

0