Next Generation dsRNA-Based Insect Control: Success So Far and Challenges

Frontiers in Plant Science, Journal Year: 2021, Volume and Issue: 12

Published: Oct. 18, 2021

RNA interference (RNAi) is a method of gene silencing where dsRNA digested into small interfering (siRNA) in the presence enzymes. These siRNAs then target homologous mRNA sequences aided by RNA-induced complex (RISC). The mechanism uptake has been well studied and established across many living organisms including insects. In insects, RNAi novel potential tool to develop future pest management means targeting various classes insects dipterans, coleopterans, hemipterans, lepidopterans, hymenopterans isopterans. However, extent individual class varies due underlying mechanisms. present review focuses on three major insect viz lepidopterans coleopterans rationale behind this lies fact that studies pertaining extensively performed these groups. Additionally, harbour agriculturally important species which require attention. Interestingly, all exhibit varying levels efficiencies with exhibiting maximum response, while hemipterans are relatively inefficient. Lepidopterans other hand, show minimum response RNAi. This attributed facts few being endosomal escape, high activity dsRNA-specific nucleases, highly alkaline gut environment renders unstable. Various methods have ensure safe delivery biological system insect. most common for administration supplementing diet via spraying onto leaves commonly eaten parts plant. environment-friendly superior hazardous effects pesticides. Another involves submergence root systems solutions subsequent phloem. more recent techniques nanoparticle- Agrobacterium -mediated systems. novelty biotechnological recalcitrant nature certain crops, further optimization required. emphasizes developments hurdles efficient also discusses detail development new enhance efficiency using liposomes nanoparticles, transplastomics, microbial-mediated chemical methods.

Language: Английский

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CRISPR‐mediated mutagenesis of the odorant receptor co‐receptor (Orco) gene disrupts olfaction‐mediated behaviors in Bactrocera dorsalis

Insect Science, Journal Year: 2022, Volume and Issue: 29(5), P. 1275 - 1286

Published: Jan. 5, 2022

Olfaction plays an essential role in insect behavior such as host location, foraging, mating, and oviposition. The odorant receptor co-receptor (Orco) is obligatory indispensable odor perception. Here, we characterized the Orco gene from oriental fruit fly, Bactrocera dorsalis (Hendel), a notorious agriculture pest. olfactory deficiency mutants were generated by editing BdorOrco using CRISPR/Cas9 system. Electroantennograms (EAG) preference assays confirmed that BdorOrco-/- mutant flies had reduced perception of methyl eugenol, β-caryophyllene, ethyl acetate. Oviposition bioassays showed eggs laid females mediated benzothiazole 1-octen-3-ol significantly decreased. In addition, took longer time to locate food source compared with wild type (WT) flies. Altogether, our data indicated for multiple physiological processes B. dorsalis, it expands understanding function Orco.

Language: Английский

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Olfactory receptors in Bactrocera species for sustainable fruit fly management: A review and future perspectives

Physiological Entomology, Journal Year: 2024, Volume and Issue: 49(2), P. 67 - 90

Published: Jan. 24, 2024

Abstract Molecular studies on odorant receptors (ORs), odorant‐binding proteins (OBPs) and the functioning of receptor pheromone signal transduction in fruit fly Bactrocera species have expanded exponentially during past few decades. OBPs contribute to sensing olfactory system (OS) via odorants through sensillum lymph. However, ORs, a family G‐protein‐coupled various other species, exhibit heightened responsiveness multiple chemical odours such as hormones, sensory stimuli neurotransmitters. The apparent mechanism involves combinatorial code encompassing both peripheral antennal lobe processing, facilitating reception sexual pheromones environmental cues. OS is specifically designed recognize process information from volatile signals, these signals play an important function flies. Insects rely chemicals navigate comprehend their surroundings. A mature insect composed two pairs sensillae‐covered palps, antennae primary appendages anterior head. It has been shown that chemosensory gene families odour perception. These include neuroreceptor families, OBPs, neuron membrane proteins. Additionally, there are three divergent chemoreceptors, namely ionotropic gustatory receptors. Methods based systematic biology, molecular biology bioinformatics tools rapidly emerged investigate communication systems provide new insights for management many agricultural pest. Several aromatic compounds, including semiochemicals pheromones, employed defend crops animals destructive flies invasive frugivorous species. To promote expansion cropping system, utilization phytochemical lures can be convenient sustainable agriculture production enhance food security. Hence, this review examined state art insects with focus pest identify semiochemical receptors, protein (CSRs), well practical applications biological control integrated highlighted.

Language: Английский

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Control of two insect pests by expression of a mismatch corrected double‐stranded RNA in plants

Plant Biotechnology Journal, Journal Year: 2024, Volume and Issue: 22(7), P. 2010 - 2019

Published: March 1, 2024

Summary RNA interference (RNAi) has emerged as an efficient technology for pest control by silencing the essential genes of targeted insects. Owing to its nucleotide sequence‐guided working mechanism, RNAi a high degree species‐specificity without impacts on non‐target organisms. However, plants are inevitably under threat two or more insect pests in nature, species‐specific mode RNAi‐based restricts wide application control. In this study, we artificially designed intermediate dsRNA ( iACT ) targeting β‐Actin ACT sap‐sucking Bemisia tabaci and Myzus persicae mutual correction their mismatches. When expressing hairpin (hp from tobacco nuclear genome, transgenic well protected both B. M. , either individually simultaneously, evidenced reduced fecundity suppressed gene expression, whereas expression hpRNA BtACT MpACT could only confer specific resistance respectively. sum, our data provide novel proof‐of‐concept that different species be simultaneously controlled artificial synthesis with sequence optimization, which expands range methods crop protection.

Language: Английский

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Chitosan double‐stranded RNA nanocomplexes for Piezodorus guildinii control

Pest Management Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 5, 2025

Piezodorus guildinii (Westwood, Hemiptera: Pentatomidae) causes economic losses in soybean crops by diminishing productivity, impacting grain size, oil content, and germination power. RNA interference (RNAi), a conserved gene regulation process initiated double-stranded (dsRNA), may be used as safe technique integrated pest management strategies. Conjugating dsRNA with cationic polymers, which protect from degradation has shown to enhance this response. This study aims assess the impact of administration on viability P. evaluate dsRNA-chitosan nanocomplexes control strategy. Ten target genes were selected, dsRNAs designed synthesized. In injection assays, significant mortality exceeding 76% was observed, while ingestion also showed differences, reaching 49%. The targeting Srp54k selected chitosan-dsRNA nanocomplex These demonstrated enhanced stability under RNase treatment vitro, and, vivo cumulative corrected rate 66% reported. Consistent these findings, RT-qPCR analysis revealed that expression levels treated insects significantly reduced compared at 24, 48, 72 h post for targeted genes. work, we evaluated effect determined its molecular silencing effects. Additionally, synthesized characterized nanoparticles, improved efficacy terms stability. findings are promising development new strategies stink bugs control. © 2025 Society Chemical Industry.

Language: Английский

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RNA interference (RNAi) applications to the management of fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae): Its current trends and future prospects

Frontiers in Molecular Biosciences, Journal Year: 2022, Volume and Issue: 9

Published: Sept. 7, 2022

The fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae) is among the invasive insect pests that damages maize and sorghum, high-priority crops in newly colonized agro-ecologies, including African contexts. Owing to increasing infestation of pest limitations current conventional methods for its management, there a call discovering advanced management approaches. RNA interference (RNAi) an emerging molecular tool showing flexible potential S. . We conducted search recent application RNAi literature using Google Scholar Mendeley find papers on tools led growth inhibition, developmental aberrations, reduced fecundity, mortality, mainly by disruption normal biological processes pest. Although efforts have been made accelerate utility RNAi, many factors limit efficiency achieve successful control over RNAi’s bioactivity economic ecological acceptability, continued research should focus improving broad applicability, field conditions. Screening identification key target genes be priority task effective sustainable this via RNAi. In addition, clear understanding present status utilization paramount importance improve efficiency. Therefore, review, we highlight biology mechanism as foundation Then, discuss knowledge approach affecting application. Finally, prospects RNAi-based are highlighted future frugiperda.

Language: Английский

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Postcopulatory Behavior of Tephritid Flies

Annual Review of Entomology, Journal Year: 2022, Volume and Issue: 68(1), P. 89 - 108

Published: Oct. 6, 2022

Mating produces profound changes in the behavior of female flies, such as an increase oviposition, reduction sexual receptivity, feeding, and even excretion. Many these are produced by copulation, sperm, accessory gland products that males transfer to females during mating. Our knowledge on function male ejaculate its effect insects is still incipient. In this article, we review peri- postcopulatory behaviors tephritid flies. We address effects copulatory behavior; copula duration; ejaculate, remating behavior. species from families pests economic importance; thus, understanding mating contributes both developing more effective environmentally friendly control methods furthering our evolutionary implications intersexual competition conflict.

Language: Английский

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Genome report: chromosome-scale genome assembly of the West Indian fruit fly Anastrepha obliqua (Diptera: Tephritidae)

G3 Genes Genomes Genetics, Journal Year: 2024, Volume and Issue: 14(4)

Published: Feb. 1, 2024

The West Indian fruit fly, Anastrepha obliqua, is a major pest of mango in Central and South America attacks more than 60 species host fruits. To support current genetic genomic research on A. we sequenced the genome using high-fidelity long-read sequencing. This resulted highly contiguous contig assembly with 90% 10 contigs. was placed chromosomal context synteny closely related species, ludens, as both are members fraterculus group. resulting represents five autosomes X chromosome which 95.9% genome, 199 unplaced contigs representing remaining 4.1%. Orthology analysis across structural annotation sets high quality tephritid genomes demonstrates gene annotations robust, identified genes unique to that may help define their pestiferous nature can be used starting point for comparative genomics. first this will serve foundation future its management an agricultural pest.

Language: Английский

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Identification of RNAi efficiency‐related gene in the green peach aphid, Myzus persicae (Hemiptera), based on comparative transcriptomics

Pest Management Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 9, 2025

Abstract BACKGROUND RNA interference (RNAi) is a promising strategy for aphid control; however, its practical application has been limited by low efficiency in aphids. To address this, we combined transcriptomic analysis with an ‘RNAi of RNAi’ approach to identify genes associated RNAi green peach Myzus persicae . RESULTS Using the artificial diet‐mediated dsRNA delivery method, observed that ingestion both dsMpHunchback and dsfusion (a targeting three genes) induced effects. These included significant gene silencing reduced nymph production at 24, 36, 48 h post‐feeding compared dsGFP. Expression profiling core machinery revealed samples from 36 were critical efficiency, prompting their selection transcriptome sequencing. Weighted co‐expression network (WGCNA) Differentially Expressed Genes (DEGs) employed elucidate RNA‐seq data. Subsequent validation through assay demonstrated functional roles encoding basic region leucine zipper ( gene11325 ), cathepsin B‐like gene12476 glucosidase KIAA1161‐like gene6489 piggyBac transposable element‐derived protein 3‐like gene7736 ). Notably, whereas g ene12476 appeared exert compensatory effects counteracted dsfusion. CONCLUSION findings highlight diversity influencing aphid, enhancing our understanding mechanisms establishing foundation optimizing RNAi‐based control strategies. © 2025 Society Chemical Industry.

Language: Английский

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Transcription and functional analysis of phosphine fumigation regulating heat responses in Bactrocera dorsalis

Pesticide Biochemistry and Physiology, Journal Year: 2025, Volume and Issue: 212, P. 106433 - 106433

Published: April 29, 2025

Language: Английский

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