A novel bHLH transcription factor, FabHLH110, is involved in regulation of anthocyanin synthesis in petals of pink-flowered strawberry

Plant Physiology and Biochemistry, Год журнала: 2025, Номер 222, С. 109713 - 109713

Опубликована: Фев. 28, 2025

Язык: Английский

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The E3 ubiquitin ligase BRG3 and the protein kinase MPK7 antagonistically regulate LBD36 turnover, a key node for integrating nitrate and gibberellin signaling in apple

New Phytologist, Год журнала: 2025, Номер unknown

Опубликована: Март 14, 2025

Summary Nitrate is the main source of nitrogen in plants. stimulation causes changes plant secondary metabolites, including anthocyanins. However, molecular mechanism underlying how nitrate regulates anthocyanin biosynthesis remains unclear. In this study, we identified a response factor MdLBD36 apple. This positively regulated deficiency‐induced by promoting transcriptional activity MdABI5, an important regulator anthocyanins, and directly activated MdABI5 expression. The E3 ubiquitin ligase MdBRG3 promoted ubiquitinated degradation to reduce under nitrate‐sufficient conditions. deficiency‐activated MdMPK7 maintained stimulating effect on counteracting MdBRG3‐mediated MdLBD36. coordinated gibberellin (GA) signaling regulate biosynthesis. GA repressor MdRGL2a contributed MdLBD36‐promoted enhancing MdLBD36–MdABI5 interaction increasing activation . summary, our results elucidate framework regulation ubiquitination phosphorylation. study revealed cross talk between provides references for in‐depth exploration signal transduction pathway its interactions with hormones.

Язык: Английский

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The MdNAC72‐MdABI5 module acts as an interface integrating jasmonic acid and gibberellin signals and undergoes ubiquitination‐dependent degradation regulated by MdSINA2 in apple

New Phytologist, Год журнала: 2024, Номер 243(3), С. 997 - 1016

Опубликована: Июнь 7, 2024

Jasmonic acid (JA) and gibberellin (GA) coordinately regulate plant developmental programs environmental cue responses. However, the fine regulatory network of cross-interaction between JA GA remains largely elusive. In this study, we demonstrate that MdNAC72 together with MdABI5 positively regulates anthocyanin biosynthesis through an exquisite MdNAC72-MdABI5-MdbHLH3 transcriptional cascade in apple. interacts to promote activation on its target gene MdbHLH3 directly activates transcription MdABI5. The MdNAC72-MdABI5 module integration signals by combining repressor MdJAZ2 MdRGL2a. disrupts interaction attenuates MdNAC72. MdRGL2a sequesters from MdJAZ2-MdNAC72 protein complex, leading release E3 ubiquitin ligase MdSINA2 is responsive promotes ubiquitination-dependent degradation interface fine-regulates at posttranslational levels MdJAZ2, MdRGL2a, MdSINA2. summary, our findings elucidate connecting as core

Язык: Английский

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LcbHLH107 actively suppresses the expression of LcDFR to repress anthocyanin biosynthesis in Litchi chinensis Sonn.

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 142396 - 142396

Опубликована: Март 1, 2025

Язык: Английский

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The basic helix-loop-helix transcription factor PpeUNE12 regulates peach ripening by promoting polyamine catabolism and anthocyanin synthesis

Plant Physiology and Biochemistry, Год журнала: 2025, Номер 220, С. 109537 - 109537

Опубликована: Янв. 21, 2025

Язык: Английский

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CsPHYB–CsPIF3/4 Regulates Hypocotyl Elongation by Coordinating the Auxin and Gibberellin Biosynthetic Pathways in Cucumber (Cucumis sativus L.)

Plants, Год журнала: 2025, Номер 14(3), С. 371 - 371

Опубликована: Янв. 26, 2025

Hypocotyl length is closely related to quality in seedlings and an important component of plant height vital for plant-type breeding cucumber. However, the underlying molecular mechanisms hypocotyl elongation are poorly understood. In this study, endogenous hormone content indole acetic acid (IAA) gibberellin (GA3) showed increase long Csphyb (phytochrome B) mutant AM274M compared with its wild-type AM274W. An RNA-sequencing analysis identified 1130 differentially expressed genes (DEGs), which 476 654 were up- downregulated AM274M, respectively. A KEGG enrichment exhibited that these DEGs mainly enriched signal transduction pathway. The expression levels pivotal CsGA20ox-2, biosynthesis pathway, CsYUCCA8, auxin notably elevated contrast Additionally, GUS staining a dual-luciferase reporter assay corroborated phytochrome-interacting factors CsPIF3/4 can bind E(G)-box motifs present promoters CsGA20ox-2 CsYUCCA8 genes, thereby modulating their subsequently influencing elongation. Consequently, research offers profound insights into regulation by response light signals establishes crucial theoretical groundwork cultivating robust cucumber agricultural practice.

Язык: Английский

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The Gmbhlh13-Gmchs7 Module Positively Regulates Isoflavones Accumulation in Soybean (Glycine Max. L)

Опубликована: Янв. 1, 2025

Язык: Английский

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MdZFP7 integrates JA and GA signals via interaction with MdJAZ2 and MdRGL3a in regulating anthocyanin biosynthesis and undergoes degradation by the E3 ubiquitin ligase MdBRG3

Journal of Integrative Plant Biology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 12, 2025

ABSTRACT Jasmonic acid (JA) and gibberellin (GA) coordinate many aspects of plant growth development, including anthocyanin biosynthesis. However, the crossover points JA GA signals pathways through which they interact to regulate biosynthesis are poorly understood. Here, we investigated molecular mechanism by zinc finger protein (ZFP) transcription factor Malus domestica ZFP7 (MdZFP7) regulates integrating at transcriptional post‐translational levels. MdZFP7 is a positive regulator biosynthesis, fulfills its role directly activating expression MdMYB1 enhancing activation MdWRKY6 on target genes MdDFR MdUF3GT . integrates interacting with repressor apple JASMONATE ZIM‐DOMAIN2 (MdJAZ2) REPRESSOR‐of‐ga1‐3‐like 3a (MdRGL3a). MdJAZ2 weakens disrupts MdZFP7–MdWRKY6 interaction, thereby reducing promoted MdZFP7. MdRGL3a contributes stimulation sequestering from MdJAZ2–MdZFP7 complex. The E3 ubiquitin ligase BOI‐related ubiquitin‐protein 3 (MdBRG3), antagonistically regulated GA, targets ubiquitination degradation MdBRG3‐MdZFP7 module moves crosstalk realm regulation into modification. In conclusion, this study not only elucidates node‐role in integration signals, but also describes regulatory network as hub.

Язык: Английский

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Exogenous Gibberellin Delays Postharvest Leaf Senescence in Pak Choi by Modulating Transcriptomic and Metabolomic Profiles

Foods, Год журнала: 2025, Номер 14(6), С. 981 - 981

Опубликована: Март 13, 2025

Postharvest leaf senescence is a pivotal determinant influencing the quality and shelf life of leafy vegetables, exemplified by pak choi (Brassica rapa L. subsp. chinensis). While regulatory role gibberellin (GA) in modulating has been documented across diverse plant species, underlying physiological molecular mechanisms remain insufficiently characterized. This study, through combination transcriptomic metabolomic analyses, investigated effect exogenous GA on postharvest choi. treatment alleviated etiolation, maintained chlorophyll levels, reduced conductivity malondialdehyde content, delayed onset symptoms Transcriptome profiling indicated that suppressed expression senescence-associated genes BraSRGs BraSAGs. In addition, influenced degradation preserved content implicated metabolism, including BraPPH, BraSGR1, BraNYCI, BraPAO. impacted lipid levels regulated membrane phospholipids. Furthermore, disrupted efficacy jasmonic acid signal pathway, primarily transcriptional downregulation key genes, BraJAZ10 BraJAR1. These results provide insights into delaying highlight potential targets for improving management vegetables.

Язык: Английский

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Advances in the biosynthesis and regulatory mechanisms of anthocyanins in horticultural plants: a comprehensive review

Tropical Plants, Год журнала: 2025, Номер 4(1), С. 0 - 0

Опубликована: Янв. 1, 2025

Язык: Английский

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