Impact of Hormone on Growth and GA3 Regulation of Anthocyanin Biosynthesis in Suspension‐Culture Cells of Cyclocarya paliurus DOI Open Access

Chuanqing Pan,

Yuan Liu,

Daobang Tang

et al.

Biotechnology and Bioengineering, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 23, 2024

ABSTRACT Hormones are effective in regulating plant metabolism and, therefore, often used cell culture to increase the yield of target products. This study investigated effects hormones on growth and anthocyanin biosynthesis suspension‐cultured red Cyclocarya paliurus cells. Additionally, mechanism by which gibberellin induces was explored through multi‐omics integrated analysis assay dynamic changes signaling molecule concentration. The results showed that total content cells, when induced 1.0 mg L⁻¹ Gibberellin A 3 (GA ), experienced increases 1.92‐ 1.83‐fold, respectively. application exogenous GA activated synthesis transduction four molecules, is, nitric oxide (NO), hydrogen peroxide (H 2 O salicylic acid (SA), jasmonic (JA), cells altered expression patterns transcription factors. factors upregulated biosynthetic genes such as anthocyanin‐3‐O‐glucosyl transferase leucoanthocyanidin dioxygenase , while downregulated reductase flavonoid 3′,5′,‐hydroxylase pathway, ultimately leading a significant biosynthesis. research work establishes foundation for further role

Language: Английский

A novel bHLH transcription factor, FabHLH110, is involved in regulation of anthocyanin synthesis in petals of pink-flowered strawberry DOI

Song Yang,

Ming Liu, Can Zhao

et al.

Plant Physiology and Biochemistry, Journal Year: 2025, Volume and Issue: 222, P. 109713 - 109713

Published: Feb. 28, 2025

Language: Английский

Citations

1
The E3 ubiquitin ligase BRG3 and the protein kinase MPK7 antagonistically regulate LBD36 turnover, a key node for integrating nitrate and gibberellin signaling in apple DOI Open Access

Xin‐Long Guo,

Da‐Ru Wang, Baoyou Liu

et al.

New Phytologist, Journal Year: 2025, Volume and Issue: unknown

Published: March 14, 2025

Summary Nitrate is the main source of nitrogen in plants. stimulation causes changes plant secondary metabolites, including anthocyanins. However, molecular mechanism underlying how nitrate regulates anthocyanin biosynthesis remains unclear. In this study, we identified a response factor MdLBD36 apple. This positively regulated deficiency‐induced by promoting transcriptional activity MdABI5, an important regulator anthocyanins, and directly activated MdABI5 expression. The E3 ubiquitin ligase MdBRG3 promoted ubiquitinated degradation to reduce under nitrate‐sufficient conditions. deficiency‐activated MdMPK7 maintained stimulating effect on counteracting MdBRG3‐mediated MdLBD36. coordinated gibberellin (GA) signaling regulate biosynthesis. GA repressor MdRGL2a contributed MdLBD36‐promoted enhancing MdLBD36–MdABI5 interaction increasing activation . summary, our results elucidate framework regulation ubiquitination phosphorylation. study revealed cross talk between provides references for in‐depth exploration signal transduction pathway its interactions with hormones.

Language: Английский

Citations

1
LcbHLH107 actively suppresses the expression of LcDFR to repress anthocyanin biosynthesis in Litchi chinensis Sonn. DOI
Shiqi Jing,

Yingsheng Xia,

Bo Zhang

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142396 - 142396

Published: March 1, 2025

Language: Английский

Citations

1
The MdNAC72‐MdABI5 module acts as an interface integrating jasmonic acid and gibberellin signals and undergoes ubiquitination‐dependent degradation regulated by MdSINA2 in apple DOI

Hong‐Liang Li,

Ruirui Xu,

Xin‐Long Guo

et al.

New Phytologist, Journal Year: 2024, Volume and Issue: 243(3), P. 997 - 1016

Published: June 7, 2024

Jasmonic acid (JA) and gibberellin (GA) coordinately regulate plant developmental programs environmental cue responses. However, the fine regulatory network of cross-interaction between JA GA remains largely elusive. In this study, we demonstrate that MdNAC72 together with MdABI5 positively regulates anthocyanin biosynthesis through an exquisite MdNAC72-MdABI5-MdbHLH3 transcriptional cascade in apple. interacts to promote activation on its target gene MdbHLH3 directly activates transcription MdABI5. The MdNAC72-MdABI5 module integration signals by combining repressor MdJAZ2 MdRGL2a. disrupts interaction attenuates MdNAC72. MdRGL2a sequesters from MdJAZ2-MdNAC72 protein complex, leading release E3 ubiquitin ligase MdSINA2 is responsive promotes ubiquitination-dependent degradation interface fine-regulates at posttranslational levels MdJAZ2, MdRGL2a, MdSINA2. summary, our findings elucidate connecting as core

Language: Английский

Citations

6
The basic helix-loop-helix transcription factor PpeUNE12 regulates peach ripening by promoting polyamine catabolism and anthocyanin synthesis DOI
Wei Wang, Chen Yang,

Yabo Jiang

et al.

Plant Physiology and Biochemistry, Journal Year: 2025, Volume and Issue: 220, P. 109537 - 109537

Published: Jan. 21, 2025

Language: Английский

Citations

0
CsPHYB–CsPIF3/4 Regulates Hypocotyl Elongation by Coordinating the Auxin and Gibberellin Biosynthetic Pathways in Cucumber (Cucumis sativus L.) DOI Creative Commons
Liqin Chen,

Zongbo Qiu,

Jing Dong

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(3), P. 371 - 371

Published: Jan. 26, 2025

Hypocotyl length is closely related to quality in seedlings and an important component of plant height vital for plant-type breeding cucumber. However, the underlying molecular mechanisms hypocotyl elongation are poorly understood. In this study, endogenous hormone content indole acetic acid (IAA) gibberellin (GA3) showed increase long Csphyb (phytochrome B) mutant AM274M compared with its wild-type AM274W. An RNA-sequencing analysis identified 1130 differentially expressed genes (DEGs), which 476 654 were up- downregulated AM274M, respectively. A KEGG enrichment exhibited that these DEGs mainly enriched signal transduction pathway. The expression levels pivotal CsGA20ox-2, biosynthesis pathway, CsYUCCA8, auxin notably elevated contrast Additionally, GUS staining a dual-luciferase reporter assay corroborated phytochrome-interacting factors CsPIF3/4 can bind E(G)-box motifs present promoters CsGA20ox-2 CsYUCCA8 genes, thereby modulating their subsequently influencing elongation. Consequently, research offers profound insights into regulation by response light signals establishes crucial theoretical groundwork cultivating robust cucumber agricultural practice.

Language: Английский

Citations

0
The Gmbhlh13-Gmchs7 Module Positively Regulates Isoflavones Accumulation in Soybean (Glycine Max. L) DOI
Tianyi Liu, Fan Yan, Yajing Liu

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
MdZFP7 integrates JA and GA signals via interaction with MdJAZ2 and MdRGL3a in regulating anthocyanin biosynthesis and undergoes degradation by the E3 ubiquitin ligase MdBRG3 DOI Open Access

Xing‐Long Ji,

L. Zhao,

Baoyou Liu

et al.

Journal of Integrative Plant Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 12, 2025

ABSTRACT Jasmonic acid (JA) and gibberellin (GA) coordinate many aspects of plant growth development, including anthocyanin biosynthesis. However, the crossover points JA GA signals pathways through which they interact to regulate biosynthesis are poorly understood. Here, we investigated molecular mechanism by zinc finger protein (ZFP) transcription factor Malus domestica ZFP7 (MdZFP7) regulates integrating at transcriptional post‐translational levels. MdZFP7 is a positive regulator biosynthesis, fulfills its role directly activating expression MdMYB1 enhancing activation MdWRKY6 on target genes MdDFR MdUF3GT . integrates interacting with repressor apple JASMONATE ZIM‐DOMAIN2 (MdJAZ2) REPRESSOR‐of‐ga1‐3‐like 3a (MdRGL3a). MdJAZ2 weakens disrupts MdZFP7–MdWRKY6 interaction, thereby reducing promoted MdZFP7. MdRGL3a contributes stimulation sequestering from MdJAZ2–MdZFP7 complex. The E3 ubiquitin ligase BOI‐related ubiquitin‐protein 3 (MdBRG3), antagonistically regulated GA, targets ubiquitination degradation MdBRG3‐MdZFP7 module moves crosstalk realm regulation into modification. In conclusion, this study not only elucidates node‐role in integration signals, but also describes regulatory network as hub.

Language: Английский

Citations

0
Exogenous Gibberellin Delays Postharvest Leaf Senescence in Pak Choi by Modulating Transcriptomic and Metabolomic Profiles DOI Creative Commons
Dan Wang,

Xiuyun Zhao,

Tongbing Su

et al.

Foods, Journal Year: 2025, Volume and Issue: 14(6), P. 981 - 981

Published: March 13, 2025

Postharvest leaf senescence is a pivotal determinant influencing the quality and shelf life of leafy vegetables, exemplified by pak choi (Brassica rapa L. subsp. chinensis). While regulatory role gibberellin (GA) in modulating has been documented across diverse plant species, underlying physiological molecular mechanisms remain insufficiently characterized. This study, through combination transcriptomic metabolomic analyses, investigated effect exogenous GA on postharvest choi. treatment alleviated etiolation, maintained chlorophyll levels, reduced conductivity malondialdehyde content, delayed onset symptoms Transcriptome profiling indicated that suppressed expression senescence-associated genes BraSRGs BraSAGs. In addition, influenced degradation preserved content implicated metabolism, including BraPPH, BraSGR1, BraNYCI, BraPAO. impacted lipid levels regulated membrane phospholipids. Furthermore, disrupted efficacy jasmonic acid signal pathway, primarily transcriptional downregulation key genes, BraJAZ10 BraJAR1. These results provide insights into delaying highlight potential targets for improving management vegetables.

Language: Английский

Citations

0
Advances in the biosynthesis and regulatory mechanisms of anthocyanins in horticultural plants: a comprehensive review DOI
Kunkun Zhao,

Q. Zhang,

Yuting Wang

et al.

Tropical Plants, Journal Year: 2025, Volume and Issue: 4(1), P. 0 - 0

Published: Jan. 1, 2025

Language: Английский

Citations

0