Divergent MYB paralogs determine spatial distribution of linalool mediated by JA and DNA demethylation participating in aroma formation and cold tolerance of tea plants

Plant Biotechnology Journal, Год журнала: 2025, Номер unknown

Опубликована: Фев. 11, 2025

Summary Linalool not only is one of characteristic flavour volatiles tea, contributing to floral aroma, but also a kind defensive compounds, playing essential roles in resistance against biotic/abiotic stresses. Although the linalool synthases have been identified, much unknown about regulation mechanism tea plants. We identified two pairs MYB paralogs as biosynthesis activators, which pair (CsMYB148/CsMYB193) specifically expressed flowers, and another (CsMYB68/CsMYB147) highly leaves, fruits roots. These activators interacted with CsMYC2 form MYC2‐MYB complexes regulate synthase . While Jasmonate ZIM‐domain (JAZ) proteins served repressors by interfering complex. Further, we found that transcripts CsMYB68 / CsMYB147 were significantly upregulated jasmonic acid (JA) improve products during processing pathway may downstream pathways JA signalling DNA methylation processes participate cold resistance. Under stress, was activated elevate abundance MYC‐MYB complexes; meanwhile, demethylation activated, leading declining levels increasing Our study provides new insight into synergistically improving quality plant

Язык: Английский

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A CCA1-like MYB subfamily member CsMYB128 participates in chilling sensitivity and cold tolerance in tea plants (Camellia sinensis)

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 139473 - 139473

Опубликована: Янв. 1, 2025

Язык: Английский

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Natural variation in promoters of F3′5′H and ANS correlates with catechins diversification in Thea species of genus Camellia

The Plant Journal, Год журнала: 2025, Номер 121(6)

Опубликована: Март 1, 2025

SUMMARY Catechins were diversely accumulated in Thea plants and crucial for tea flavor, yet the mechanism underlying diverse catechins distribution remained elusive. We herein collected a total of 19 12 non‐ to investigate their mechanism. Results showed that pattern cultivated significantly differs from wild relatives. (+)‐Gallocatechin gallate (GCG) was detected over 50% but almost undetectable plants. Conversely, (−)‐Epigallocatechin (EGCG) extensively distributed cultivars extremely low few relatives such as Camellia tetracocca C. ptilophylla . Expression analysis found expression flavonoid 3′,5′‐hydroxylase ( F3′5′H ) highly correlated with EGCG accumulation Yeast one‐hybrid luciferase assays CsMYB1, key regulator, could bind promoter activate its promote plants; it unable due 14‐bp deletion promoter, leading content EGCG. also silencing anthocyanidin synthase ANS enhanced metabolic flux toward GCG not plants, consistent observation high expression. Overall, results illustrated variation would help facilitate utilization future breeding.

Язык: Английский

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Revealing novel insights into the improvement of greenhouse tea quality through exogenous substance interventions using targeted and untargeted metabolomics and microbial community analyses

Food Chemistry X, Год журнала: 2025, Номер unknown, С. 102410 - 102410

Опубликована: Март 1, 2025

Tea quality in greenhouse was certain gap with open air. Metabolites and foliar microorganisms were investigated under seaweed fertiliser (CF) gibberellin (CH) treatments using sensory evaluation, HPLC, untargeted metabolomics, 16S rDNA, Internal Transcribed Spacer. CF tea mellow, less astringent, of better compared to CH. Catechin, -(-)Epicatechin, Epigallocatechin notably lower CF. Differentially accumulated metabolites (DAMs) enriched Flavonoid Phenylpropanoid biosynthesis, both involved Catechin synthesis. DAMs these pathways appeared down-regulated The improved by down-regulating biosynthesis conjunction microbial community metabolism amino acid secondary metabolite biosynthesis. Metabolite- correlation analysis indicated that the highest phenylpropane pathway bacteria Variovorax Pseudomonas, fungi Filobasidium. study provides theoretical basis for regulating flavour tea.

Язык: Английский

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Effect of micrografting technique on growth and cold resistance of tea (Camellia sinensis) plant

BMC Plant Biology, Год журнала: 2025, Номер 25(1)

Опубликована: Июнь 2, 2025

Micrografting technology has gained popularity in model plants, with the advantages of a wide grafting range and small space. However, this technique not been fully explored tea plants. In our study, different rootstocks [radicle (obtained from germination seed), epicotyl without cotyledons, varieties] scion (red branch, green branch) combinations were used to estimate survival rate, plant growth, compatibility behavior, cold tolerance grafted seedlings. Our results showed that higher rate shooting observed radicle germinated seed diameter ≥ 15 mm, D3) as rootstock. Also, same growth indicators found branch rootstock (GB\R) than other combinations. addition, seedlings LJ43 had best vascular bundle bridge was completely established SCZ (SCZ/LJ43) graft combination, accompanied shoot leaf number new shoots field experiments. findings provide viable micrografting method, which potential substitute traditional cuttings for seedling propagation thus meet requirements cultivation.

Язык: Английский

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Genetic diversity analysis and core collection construction of tea plant from the Yunnan Province of China using ddRAD sequencing

BMC Plant Biology, Год журнала: 2024, Номер 24(1)

Опубликована: Дек. 4, 2024

Язык: Английский

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Optimization of Tea (Camellia Sinensis) Micrografting Technique to Enhance its Adoption in Tea Cultivation

Опубликована: Янв. 1, 2024

Язык: Английский

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