Investigating Biofilms: Advanced Methods for Comprehending Microbial Behavior and Antibiotic Resistance
Frontiers in Bioscience-Landmark,
Год журнала:
2024,
Номер
29(4), С. 133 - 133
Опубликована: Март 29, 2024
Biofilms,
which
consist
of
microorganisms
enclosed
in
an
extracellular
polymeric
material
(EPS),
hold
immense
importance
the
fields
environmental
research,
industry,
and
medicine.
They
play
a
significant
role
ecosystem
dynamics
stability,
but
they
also
pose
issues
such
as
biofouling,
corrosion,
pollution.
Biofilms
medical
environments
are
linked
to
persistent
infections
elevated
healthcare
expenses.
The
EPS
matrix
plays
crucial
maintaining
structural
integrity
antibiotic
resistance
these
structures.
research
primarily
investigates
facilitating
horizontal
gene
transfer
among
biofilm
communities,
with
particular
emphasis
on
its
impact
this
process.
process
is
recognized
pivotal
mechanism
emergence
resistance,
underscoring
function
biofilms.
analysis
highlights
financial
constraints
caused
by
biofilms
several
industries.
Biofilm-associated
sector
result
escalated
treatment
expenses
extended
hospitalization
periods.
In
industrial
context,
have
increasing
maintenance
product
contamination,
emphasizing
need
for
efficient
management
solutions.
This
review
presents
most
recent
progress
utilization
sophisticated
imaging
tools
molecular
methodologies.
addition
conventional
techniques,
explores
tools,
DNA
RNA
sequencing,
conjunction
proteomics.
These
approaches
essential
assessing
genetic
metabolic
mechanisms
that
regulate
development
resistance.
underscores
significance
employing
interdisciplinary
methodology
study
By
incorporating
range
approaches,
analysis,
comprehensive
understanding
may
be
achieved.
approach
opens
up
possibilities
developing
novel
solutions
address
negative
impacts
health,
environment.
Язык: Английский
How Do ROS Induce NETosis? Oxidative DNA Damage, DNA Repair, and Chromatin Decondensation
Biomolecules,
Год журнала:
2024,
Номер
14(10), С. 1307 - 1307
Опубликована: Окт. 16, 2024
Neutrophil
extracellular
traps
(NETs)
are
intricate,
DNA-based,
web-like
structures
adorned
with
cytotoxic
proteins.
They
play
a
crucial
role
in
antimicrobial
defense
but
also
implicated
autoimmune
diseases
and
tissue
injury.
The
process
of
NET
formation,
known
as
NETosis,
is
regulated
cell
death
mechanism
that
involves
the
release
these
unique
to
neutrophils.
NETosis
heavily
dependent
on
production
reactive
oxygen
species
(ROS),
which
can
be
generated
either
through
NADPH
oxidase
(NOX)
or
mitochondrial
pathways,
leading
NOX-dependent
NOX-independent
respectively.
Recent
research
has
revealed
an
intricate
interplay
between
ROS
production,
DNA
repair,
formation
different
contexts.
UV
radiation
trigger
combined
apoptosis,
apoNETosis,
driven
by
repair.
Similarly,
calcium
ionophore-induced
both
repair
key
components,
only
partial
role.
In
case
bacterial
infections,
early
stages
pivotal.
Interestingly,
serum-free
conditions,
spontaneous
occurs
NOX-derived
ROS,
early-stage
inhibition
halting
process,
while
late-stage
increases
it.
balance
processes
appears
critical
factor
regulating
pathways
being
activated
depending
nature
stimulus.
These
findings
not
deepen
our
understanding
mechanisms
behind
suggest
potential
therapeutic
targets
for
conditions
where
NETs
contribute
disease
pathology.
Язык: Английский
Dual‐Action Antibacterial Nanoblades for Rapid Inactivation of Bioaerosols in Personal Protective Equipment
Advanced Functional Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 31, 2025
Abstract
Commercial
personal
protective
equipment
(PPE)
often
has
limited
antibacterial
activity
that
compromises
its
effectiveness
in
preventing
infections.
Traditional
mechano‐bactericidal
methods
deliver
suboptimal
performance,
while
photo‐bactericidal
struggle
with
activation
requirements
and
durability.
In
this
study,
a
dual‐action
PPE,
combining
mechano‐
strategies,
have
been
designed
to
overcome
these
challenges.
Mg–Al
layered
double
hydroxide
nanoblades
(LDH‐NBs)
are
synthesized
on
polydimethylsiloxane
(PDMS)‐coated
polypropylene
(PP)
fabric.
The
PDMS
coating
plays
crucial
role
enabling
the
formation
of
densely
packed
LDH‐NBs,
thereby
implementing
action.
Additionally,
hydrophobic
residues
diffuse
surface
during
heat
treatment,
converting
LDH‐NB
hydrophobic.
Rose
Bengal
(RB)‐adsorbed
LDH‐NBs
(RB@LDH‐NBs)
become
superhydrophobic
after
exhibiting
antifouling
effects
against
various
contaminants
bacteria.
RB@LDH‐NBs
mechanically
inactivate
99.5%
Escherichia
coli
95%
Staphylococcus
aureus
under
typical
respiratory
flow
rates
completely
eradicate
E.
within
2
h
light
exposure.
Combining
photobactericidal
effects,
rapidly
both
S.
15
min
These
outstanding
bactericidal
performances
highlight
potential
advanced
PPE
provide
robust
protection
infectious
diseases.
Язык: Английский
Evaluating Antibiofilm Efficacy of Carbon Dots Against Based on Precursor Molecule Selection
Colloids and Surfaces B Biointerfaces,
Год журнала:
2025,
Номер
254, С. 114811 - 114811
Опубликована: Май 26, 2025
Язык: Английский
Surface Visualization of a Bacterial Biofilm using Scanning Helium Microscopy
Research Square (Research Square),
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 26, 2024
Abstract
The
scanning
helium
microscope
(SHeM)
is
an
emerging
technology
that
uses
a
beam
of
neutral
atoms
to
form
images
with
extreme
surface
sensitivity
and
non-destructive
qualities.
Here,
we
present
the
first
application
SHeM
image
bacterial
biofilms.
We
demonstrate
uniquely
natively
visualizes
outermost
extracellular
polymeric
substance
in
absence
contrast
agents
dyes
without
inducing
radiative
damage.
Язык: Английский