Cited by Surface Visualization of a Bacterial Biofilm using Scanning Helium Microscopy

Investigating Biofilms: Advanced Methods for Comprehending Microbial Behavior and Antibiotic Resistance DOI

Ahmad Almatroudi

Frontiers in Bioscience-Landmark, Journal Year: 2024, Volume and Issue: 29(4), P. 133 - 133

Published: March 29, 2024

Biofilms, which consist of microorganisms enclosed in an extracellular polymeric material (EPS), hold immense importance the fields environmental research, industry, and medicine. They play a significant role ecosystem dynamics stability, but they also pose issues such as biofouling, corrosion, pollution. Biofilms medical environments are linked to persistent infections elevated healthcare expenses. The EPS matrix plays crucial maintaining structural integrity antibiotic resistance these structures. research primarily investigates facilitating horizontal gene transfer among biofilm communities, with particular emphasis on its impact this process. process is recognized pivotal mechanism emergence resistance, underscoring function biofilms. analysis highlights financial constraints caused by biofilms several industries. Biofilm-associated sector result escalated treatment expenses extended hospitalization periods. In industrial context, have increasing maintenance product contamination, emphasizing need for efficient management solutions. This review presents most recent progress utilization sophisticated imaging tools molecular methodologies. addition conventional techniques, explores tools, DNA RNA sequencing, conjunction proteomics. These approaches essential assessing genetic metabolic mechanisms that regulate development resistance. underscores significance employing interdisciplinary methodology study By incorporating range approaches, analysis, comprehensive understanding may be achieved. approach opens up possibilities developing novel solutions address negative impacts health, environment.

Language: Английский

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How Do ROS Induce NETosis? Oxidative DNA Damage, DNA Repair, and Chromatin Decondensation DOI

Dhia Azzouz,

Nades Palaniyar

Biomolecules, Journal Year: 2024, Volume and Issue: 14(10), P. 1307 - 1307

Published: Oct. 16, 2024

Neutrophil extracellular traps (NETs) are intricate, DNA-based, web-like structures adorned with cytotoxic proteins. They play a crucial role in antimicrobial defense but also implicated autoimmune diseases and tissue injury. The process of NET formation, known as NETosis, is regulated cell death mechanism that involves the release these unique to neutrophils. NETosis heavily dependent on production reactive oxygen species (ROS), which can be generated either through NADPH oxidase (NOX) or mitochondrial pathways, leading NOX-dependent NOX-independent respectively. Recent research has revealed an intricate interplay between ROS production, DNA repair, formation different contexts. UV radiation trigger combined apoptosis, apoNETosis, driven by repair. Similarly, calcium ionophore-induced both repair key components, only partial role. In case bacterial infections, early stages pivotal. Interestingly, serum-free conditions, spontaneous occurs NOX-derived ROS, early-stage inhibition halting process, while late-stage increases it. balance processes appears critical factor regulating pathways being activated depending nature stimulus. These findings not deepen our understanding mechanisms behind suggest potential therapeutic targets for conditions where NETs contribute disease pathology.

Language: Английский

Citations

Dual‐Action Antibacterial Nanoblades for Rapid Inactivation of Bioaerosols in Personal Protective Equipment DOI

Se Kye Park, Seung Yeon Lee, Sang Bok Kim

et al.

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 31, 2025

Abstract Commercial personal protective equipment (PPE) often has limited antibacterial activity that compromises its effectiveness in preventing infections. Traditional mechano‐bactericidal methods deliver suboptimal performance, while photo‐bactericidal struggle with activation requirements and durability. In this study, a dual‐action PPE, combining mechano‐ strategies, have been designed to overcome these challenges. Mg–Al layered double hydroxide nanoblades (LDH‐NBs) are synthesized on polydimethylsiloxane (PDMS)‐coated polypropylene (PP) fabric. The PDMS coating plays crucial role enabling the formation of densely packed LDH‐NBs, thereby implementing action. Additionally, hydrophobic residues diffuse surface during heat treatment, converting LDH‐NB hydrophobic. Rose Bengal (RB)‐adsorbed LDH‐NBs (RB@LDH‐NBs) become superhydrophobic after exhibiting antifouling effects against various contaminants bacteria. RB@LDH‐NBs mechanically inactivate 99.5% Escherichia coli 95% Staphylococcus aureus under typical respiratory flow rates completely eradicate E. within 2 h light exposure. Combining photobactericidal effects, rapidly both S. 15 min These outstanding bactericidal performances highlight potential advanced PPE provide robust protection infectious diseases.

Language: Английский

Citations

Surface Visualization of a Bacterial Biofilm using Scanning Helium Microscopy DOI

Nick A. von Jeinsen, David J. Ward, Matthew Bergin

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 26, 2024

Abstract The scanning helium microscope (SHeM) is an emerging technology that uses a beam of neutral atoms to form images with extreme surface sensitivity and non-destructive qualities. Here, we present the first application SHeM image bacterial biofilms. We demonstrate uniquely natively visualizes outermost extracellular polymeric substance in absence contrast agents dyes without inducing radiative damage.

Language: Английский

Citations