American Journal of Reproductive Immunology,
Год журнала:
2024,
Номер
91(3)
Опубликована: Март 1, 2024
Abstract
Background
Endometritis
is
an
inflammatory
reaction
of
the
lining
uterus,
leading
to
occurrence
infertility.
Platelet
rich
plasma
(PRP)
has
been
proven
exhibit
extremely
effective
for
treatment
endometrium‐associated
infertility,
but
mechanism
its
prevention
endometritis
remains
unclear.
Objective
The
present
study
aimed
investigate
protective
effect
PRP
against
induced
by
lipopolysaccharide
(LPS)
and
elucidate
underlying
these
effects.
Methods
Mouse
model
was
established
intrauterine
perfusion
LPS.
infusion
administered
at
24
h
after
LPS
induction.
After
another
h,
uterine
tissues
were
harvested
observe
histopathological
changes,
production
proinflammatory
cytokines,
variation
Toll‐like
receptor
4/nuclear
factor
κB
(TLR4/NF‐κB)
signaling
pathways,
validated
anti‐inflammatory
PRP.
myeloperoxidase
(MPO)
activity
concentration
nitric
oxide
(NO)
determined
using
assay
kit.
Proinflammatory
chemokines
(tumor
necrosis
factor‐α
(TNF‐α),
interleukin‐1β
(IL‐1β),
interleukin‐6
(IL‐6))
measured
ELISA
Real‐Time
PCR.
TLR4/NF‐κB
pathway
in
Western
blotting.
Results
Hematoxylin‐eosin
staining
(H&E)
appeared
that
remarkably
relieved
impairment
tissues.
Detection
MPO
NO
revealed
distinctly
mitigated
infiltration
cells
mice
with
significantly
affected
expression
TNF‐α,
IL‐1β,
IL‐6.
also
found
suppress
LPS‐induced
activation
pathway.
Conclusion
effectively
alleviates
via
restraining
signal
TLR4/NF‐κB.
These
findings
provide
a
solid
foundation
as
potential
therapeutic
agent
endometritis.
Endometritis
is
defined
as
an
infection
or
inflammation
of
the
endometrium.Endometritis
two
types:
acute
and
chronic.Acute
endometritis
symptomatic
endometrium,
which
upon
examination
with
a
microscope
shows
micro-abscess
neutrophil
invasion
in
superficial
endometrium.One
its
most
common
manifestations
postpartum
endometritis.Chronic
silent
disease
usually
diagnosed
on
workup
secondary
amenorrhoea
infertility.An
important
cause
chronic
tuberculosis,
especially
developing
nations.Chronic
have
been
associated
poor
reproductive
outcomes.Worse
outcomes
reported
for
individuals
endometritis.This
scoping
review
impact
fertility.
Scientific Reports,
Год журнала:
2021,
Номер
11(1)
Опубликована: Янв. 18, 2021
Abstract
The
aim
of
the
present
systematic
review
and
meta-analysis
was
to
assess
effect
different
therapeutic
options
for
repeated
embryo
implantation
failure
(RIF)
on
a
subsequent
IVF
cycle
outcome.
Twenty-two
RCTs
nineteen
observational
studies
were
included.
Pooling
results
showed
beneficial
intrauterine
PBMC
infusion
both
CPR
(RR
2.18;
95%
CI
1.58–3.00;
p
<
0.00001;
OR
2.03;
1.22–3.36;
=
0.006)
LBR
2.41;
1.40–4.16;
0.002;
3.73;
1.13–12.29;
0.03),
subcutaneous
G-CSF
administration
2.29;
1.58–3.31;
0.0001)
PRP
2.45;
1.55–3.86;
0.0001).
Observational
also
demonstrated
positive
IVIG
hCG
atosiban
CPR.
Studies
investigating
infusion,
LMWH,
intravenous
intralipid,
hysteroscopy,
blastocyst-stage
ET,
ZIFT,
PGT-A
AH
failed
observe
an
impact
quality
evidence
that
emerged
from
focused
moderate.
For
all
other
therapies/interventions
it
varied
low
very
low.
In
conclusion,
are
most
promising
RIF.
However,
further
well
conducted
necessary
before
their
introduction
into
clinical
practice.
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(3), С. 2901 - 2901
Опубликована: Фев. 2, 2023
The
expression
of
genes
various
proinflammatory
chemokines
and
cytokines
is
controlled,
among
others,
by
the
signaling
pathway
nuclear
factor
kappaB
(NF-κB)
superfamily
proteins,
providing
an
impact
on
immune
system
functioning.
present
review
addresses
influence
role
NF-κB
in
development
progression
most
vital
endometrial
diseases
human
animal
species.
Immune
modulation
endometritis,
endometrosis,
endometriosis,
carcinoma
results
changes
cell
migration,
proliferation,
inflammation
intensity
both
stroma
epithelium.
In
cells,
may
be
activated
multiple
stimuli,
such
as
bacterial
parts,
cytokines,
or
hormones
binding
to
specific
receptors.
dysregulation
response
involves
aberrant
production
which
plays
a
carcinoma.
However,
estrogen
progesterone
reproductive
tract
always
major
its
regulation.
Thus,
sex
cannot
overlooked
disease
physiopathology.
While
seems
NF-κB-dependent,
hormone-independent
hormone-dependent
regulation
endometrium
should
considered
future
studies.
Future
goals
this
research
step
up
into
clinical
trials
with
compounds
affecting
treatment
for
diseases.
Medical Review,
Год журнала:
2022,
Номер
2(6), С. 570 - 589
Опубликована: Дек. 1, 2022
Recurrent
pregnancy
loss
(RPL)
has
become
an
important
reproductive
health
issue
worldwide.
RPL
affects
about
2%-3%
of
reproductive-aged
women,
and
makes
serious
threats
to
women's
physical
mental
health.
However,
the
etiology
approximately
50%
cases
remains
unknown
(unexplained
RPL),
which
poses
a
big
challenge
for
clinical
management
these
patients.
been
widely
regarded
as
complex
disease
where
its
attributed
numerous
factors.
Heretofore,
various
risk
factors
have
identified,
such
maternal
ages,
genetic
factors,
anatomical
structural
abnormalities,
endocrine
dysfunction,
prethrombotic
state,
immunological
infection.
More
importantly,
development
applications
next
generation
sequencing
technology
significantly
expanded
opportunities
discover
chromosomal
aberrations
single
gene
variants
responsible
RPL,
provides
new
insight
into
pathogenic
mechanisms.
Furthermore,
based
upon
patients'
diagnostic
evaluation
etiologic
diagnosis,
specific
therapeutic
recommendations
established.
This
review
will
highlight
current
understanding
recent
advances
on
with
special
focus
etiologies,
diagnosis
management.
Frontiers in Cellular and Infection Microbiology,
Год журнала:
2023,
Номер
13
Опубликована: Май 22, 2023
Background
The
previous
researches
show
that
infertile
patients
have
a
higher
incidence
of
endometritis
and
endometrial
polyps,
the
occurrence
these
two
diseases
is
related
to
changes
in
microbiota
genital
tract.
We
aim
determine
composition
changing
characteristics
tract
(especially
endometrium)
with
chronic
or
find
correlation
between
it
diseases.
Methods
This
prospective
study.
collected
biopsy
samples
from
134
asymptomatic
receiving
assisted
reproductive
therapy
before
embryo
transfer.
Through
pathological
examination
16S
ribosomal
RNA(16S
rRNA)
sequencing,
we
determined
distribution
polyps
patients,
as
well
their
microorganisms.
Results
Compared
normal
control
group,
microbial
group
changed,
there
are
significant
species
differences
relative
abundance
vagina,
cervix
uterine
cavity.
Lactobacillus
,
dominant
flora
female
tract,
showed
change
Endometrial
composed
Staphylococcus,
Gardnerella,
Atopobium,
Streptococcus,
Peptostreptococcus,
Chlamydia,
Fusobacterium,
Acinetobacter
etc.
polyps.
Conclusion
results
that,
compared
did
species,
suggesting
local
microecology
may
be
an
important
factor
disease,
even
adverse
pregnancy
outcomes.
further
study
provide
new
opportunity
improve
diagnosis
treatment
strategy
endometritis.
Frontiers in Cellular and Infection Microbiology,
Год журнала:
2024,
Номер
14
Опубликована: Март 18, 2024
Relevant
studies
increasingly
indicate
that
female
reproductive
health
is
confronted
with
substantial
challenges.
Emerging
research
has
revealed
the
microbiome
interacts
anatomy,
histology,
and
immunity
of
tract,
which
are
cornerstone
maintaining
preventing
adverse
pregnancy
outcomes.
Currently,
precise
mechanisms
underlying
their
interaction
impact
on
physiological
functions
tract
remain
elusive,
constituting
a
prominent
area
investigation
within
field
microecology.
From
this
new
perspective,
we
explore
interactions
between
factors
affect
composition
in
as
well
personalized
medicine
approaches
managing
based
microbiome.
This
study
highlights
pivotal
role
influencing
occurrence
diseases.
These
findings
support
exploration
innovative
for
prevention,
monitoring
treatment
diseases
