Melatonin as a master regulatory hormone for genetic responses to biotic and abiotic stresses in model plant Arabidopsis thaliana: a comprehensive review

Functional Plant Biology, Год журнала: 2024, Номер 51(2)

Опубликована: Фев. 4, 2024

Melatonin is a naturally occurring biologically active amine produced by plants, animals and microbes. This review explores the biosynthesis of melatonin in with particular focus on its diverse roles Arabidopsis thaliana , model species. affects abiotic biotic stress resistance A. . Exogenous endogenous addressed association various conditions, including cold stress, high light intense heat infection Botrytis cinerea or Pseudomonas as well seed germination lateral root formation. Furthermore, confers initiating antioxidant system, remedying photosynthesis suppression, regulating transcription factors involved (CBF, DREB, ZAT, CAMTA, WRKY33, MYC2, TGA) other stress-related hormones (abscisic acid, auxin, ethylene, jasmonic acid salicylic acid). article additionally addresses precursors, metabolic components, expression genes (COR CBF SNAT ASMT PIN PR1 PDF1.2 HSFA ) proteins (JAZ, NPR1) associated reducing both biological environmental stressors. future perspective rich agri-crops explored to enhance plant tolerance stresses, maximise crop productivity nutritional worth, which may help improve food security.

Язык: Английский

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Exogenously Applied Melatonin and Calcium Alleviate Salt Injuries to Growth, Physiological, and Biochemical Attributes of Borago officinalis

Journal of Plant Growth Regulation, Год журнала: 2023, Номер 42(11), С. 6853 - 6869

Опубликована: Апрель 10, 2023

Язык: Английский

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Unraveling the regulatory role of MYC2 on ASMT gene expression in wheat: Implications for melatonin biosynthesis and drought tolerance

Physiologia Plantarum, Год журнала: 2023, Номер 175(5)

Опубликована: Авг. 1, 2023

Abstract Recognized for its multifaceted functions, melatonin is a hormone found in both animals and plants. In the plant kingdom, it plays diverse roles, regulating growth, development, stress responses. Notably, demonstrates significance by mitigating effects of abiotic stresses like drought. However, understanding precise regulatory mechanisms controlling biosynthesis genes, especially during monocots' response to stresses, requires further exploration. Seeking understand molecular basis drought tolerance wheat, we analyzed RNA‐Seq libraries wheat exposed using bioinformatics methods. light our findings, identified that Myelocytomatosis oncogenes 2 (MYC2) transcription factor hub gene upstream main gene, N‐acetylserotonin methyltransferase ( ASMT ), response‐gene network. Promoter analysis suggested might be target MYC2. We conducted set physiochemical assays along with robust machine learning approaches elevate those findings further. MYC2 were co‐regulated under Jasmonate, drought, combination them leaf tissues was detected. A meaningful correlation observed among expression profiles, contents, photosynthetic activities, antioxidant enzyme H O levels, plasma membrane damage. The results indicated an evident relationship between jasmonic acid pathway. Moreover, seems MYC2‐ASMT module contribute contents.

Язык: Английский

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Melatonin activates the OsbZIP79–OsABI5 module that orchestrates nitrogen and ROS homeostasis to alleviate nitrogen-limitation stress in rice

Plant Communications, Год журнала: 2023, Номер 4(6), С. 100674 - 100674

Опубликована: Авг. 19, 2023

Melatonin (Mel) has previously been reported to effectively alleviate nitrogen-limitation (N-L) stress and thus increase nitrogen-use efficiency (NUE) in several plants, but the underlying mechanism remains obscure. Here, we revealed that OsbZIP79 (BASIC LEUCINE ZIPPER 79) is transcriptionally activated under N-L conditions, its expression further enhanced by exogenous Mel. By combined use of omics, genetics, biological techniques, OsbZIP79-OsABI5 (ABSCISIC ACID INSENSITIVE 5) module stimulated regulation reactive oxygen species (ROS) homeostasis uptake metabolism nitrogen conditions indoor limitation (1/16 normal level). transcription OsABI5, OsABI5 then bound promoters target genes, including genes involved ROS metabolism, activating their transcription. This was also indispensable for upregulation other abscisic acid catabolism, uptake, assimilation Mel treatment, although these were not directly transactivated OsABI5. Field experiments demonstrated significantly improved rice growth low (L-N, half level) same study. application produced a 28.6% yield L-N similar increases NUE. Also, two OsbZIP79-overexpression lines grown field plots had higher NUE (+13.7% +21.2%) than wild types. Together, our data show an regulates response N insufficiency (N or N), which important increasing production.

Язык: Английский

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Microbial melatonin metabolism in the human intestine as a therapeutic target for dysbiosis and rhythm disorders

npj Biofilms and Microbiomes, Год журнала: 2024, Номер 10(1)

Опубликована: Ноя. 27, 2024

Abstract Melatonin (MT) (N-acetyl-5-methoxytryptamine) is an indoleamine recognized primarily for its crucial role in regulating sleep through circadian rhythm modulation humans and animals. Beyond association with the pineal gland, it synthesized various tissues, functioning as a hormone, tissue factor, autocoid, paracoid, antioxidant, impacting multiple organ systems, including gut-brain axis. However, mechanisms of extra-pineal MT production microbiota-host interactions remain less understood. This review provides comprehensive overview MT, production, actions sites, metabolic pathways, implications human health. The gastrointestinal tract highlighted additional source examination effects on intestinal microbiota. explores whether microbiota contributes to intestine, relationship food intake, Due impacts microbiota, may be valuable therapeutic agent dysbiosis-associated conditions. Moreover, due influence levels, possible target treating health disorders related dysregulation.

Язык: Английский

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The CGA1-SNAT regulatory module potentially contributes to cytokinin-mediated melatonin biosynthesis and drought tolerance in wheat

BMC Plant Biology, Год журнала: 2025, Номер 25(1)

Опубликована: Март 7, 2025

Melatonin plays a pivotal role in alleviating abiotic stresses, yet its biosynthesis regulation crops, particularly wheat, remains unclear. This study explores regulatory components of melatonin under drought stress using bioinformatic, physiochemical, and molecular approaches contrasting wheat genotypes. Bioinformatic analysis identified SNAT, key gene, 88 transcription factors (TFs) from 26 families as potential regulators. The network for SNAT highlighted CYTOKININ-RESPONSIVE GATA FACTOR 1 (CGA1) significant TF. Under stress, genotypes exhibited distinct CGA1-SNAT module expression, cytokinin levels, photosynthetic activity, oxidative damage. Cytokinin treatments regulated the module, altering content, SPAD values, chloroplast numbers, drought-susceptible uncovers interaction with pathway regulating during stress. These findings enhance our understanding mechanisms underpinning tolerance offer promising targets genetic biochemical interventions to improve crop resilience.

Язык: Английский

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The ABF4‐bHLH28‐COMT5 module regulates melatonin synthesis and root development for drought tolerance in citrus

The Plant Journal, Год журнала: 2025, Номер 121(6)

Опубликована: Март 1, 2025

SUMMARY Melatonin significantly influences the regulation of plant growth, development, and stress tolerance. However, regulatory mechanisms underlying melatonin accumulation for drought tolerance in citrus are not fully understood. In this study, we first demonstrated that application exogenous resulted better by reducing water loss maintaining redox homeostasis. Genome‐wide analysis revealed presence 96 genes involved biosynthesis trifoliate orange ( Poncirus trifoliata L., also known as Citrus L.). Seven caffeic acid‐O‐methyltransferases (COMT) were detected, among which PtCOMT5 was most substantially induced predominantly expressed roots leaves. Overexpression led to enhanced promoting root whereas CRISPR‐Cas9‐mediated mutation opposite phenotype. Yeast one‐hybrid screening protein‐DNA interaction assays confirmed transcription factor PtbHLH28 acts a transcriptional activator through interacting with gene promoter. addition, found be positively regulated PtABF4, core member ABA signaling pathway. PtABF4 function regulating ‐mediated synthesis development. Overall, study elucidates crucial role molecular module composed PtABF4‐PtbHLH28‐ modulation development citrus. Our findings shed light on plants exposed gain new insight into network associated response.

Язык: Английский

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Genome‐Wide Identification and Functional Characterization of New Serotonin N‐Acetyltransferases in Soybean

Food Science & Nutrition, Год журнала: 2025, Номер 13(4)

Опубликована: Апрель 1, 2025

ABSTRACT Melatonin is recognized as a crucial plant hormone due to its significant role in development, growth promotion, enhancing antioxidant activity, and stress resistance. Soybean one of the most important crops globally. To date, three SNAT genes have been cloned Arabidopsis rice; however, only gene, GmSNAT1 , has characterized soybeans. In this study, members GmGNAT family were identified through genome‐wide analysis. Two candidate GmSNAT ( GmSNAT3.1 GmSNAT3.2 ) selected based on phylogenetic tree analysis exhibited highly similar protein tertiary structures ZmSNAT3. Furthermore, functionality these two was confirmed for synthesizing N‐acetylserotonin or melatonin using serotonin 5‐methoxytryptamine substrates vitro. RNA‐seq data indicated that transcription levels downregulated under conditions dehydration salt stress. Additionally, SNAT3 from other leguminous plants homology with genes. This study elucidates soybeans establishes foundation further exploration into physiological functions within legume plants.

Язык: Английский

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Melatonin in crop plants: from biosynthesis through pleiotropic effects to enhanced stress resilience

Journal of Applied Genetics, Год журнала: 2025, Номер unknown

Опубликована: Апрель 30, 2025

Язык: Английский

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Effects of melatonin and tryptophan applications on viability and germination performance of tomato seeds during and after artificial aging

Acta Scientiarum Polonorum Hortorum Cultus, Год журнала: 2025, Номер 24(2), С. 33 - 52

Опубликована: Апрель 30, 2025

Although the diurnal fluctuations of melatonin (Mel) content in plants and its role abiotic biotic stress tolerance are well-documented, little is known about changes within seeds potential effects on seed viability or aging process. This study aimed to determine how artificial aging, induced by a controlled deterioration test, affects Mel tryptophan (Trp) viability. Furthermore, evaluated Trp applications mitigating impacts artificially aged seeds. Tomato treated with 250 µM were for up 8 days through test after which during ageing effect treatments germination performance was determined. Seeds also following order The positive vigor particularly evident compared control It observed that subjected contents exhibited an opposite trend. Applications precursor Trp, before significantly slowed down process alleviated adverse protecting membrane structures against peroxidation accumulation malondialdehyde (MDA) H2O2. Moreover, indicators such as electrical conductivity, MDA, H2O2 reduced untreated seeds, while activities antioxidant enzymes boosted. In conclusion, importance preserving viability, minimizing storage losses, slowing has been demonstrated, suggesting practical applications, endangered species valuable breeding materials.

Язык: Английский

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