Plant Cell Reports, Journal Year: 2024, Volume and Issue: 43(11)
Published: Oct. 28, 2024
Language: Английский
Physiologia Plantarum, Journal Year: 2023, Volume and Issue: 175(5)
Published: Aug. 1, 2023
Abstract Recognized for its multifaceted functions, melatonin is a hormone found in both animals and plants. In the plant kingdom, it plays diverse roles, regulating growth, development, stress responses. Notably, demonstrates significance by mitigating effects of abiotic stresses like drought. However, understanding precise regulatory mechanisms controlling biosynthesis genes, especially during monocots' response to stresses, requires further exploration. Seeking understand molecular basis drought tolerance wheat, we analyzed RNA‐Seq libraries wheat exposed using bioinformatics methods. light our findings, identified that Myelocytomatosis oncogenes 2 (MYC2) transcription factor hub gene upstream main gene, N‐acetylserotonin methyltransferase ( ASMT ), response‐gene network. Promoter analysis suggested might be target MYC2. We conducted set physiochemical assays along with robust machine learning approaches elevate those findings further. MYC2 were co‐regulated under Jasmonate, drought, combination them leaf tissues was detected. A meaningful correlation observed among expression profiles, contents, photosynthetic activities, antioxidant enzyme H O levels, plasma membrane damage. The results indicated an evident relationship between jasmonic acid pathway. Moreover, seems MYC2‐ASMT module contribute contents.
Language: Английский
Citations
11
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(12), P. 6799 - 6799
Published: June 20, 2024
Melatonin (MEL), a hormone primarily known for its role in regulating sleep and circadian rhythms animals, has emerged as multifaceted molecule plants. Recent research shed light on diverse functions plant growth defense mechanisms. This review explores the intricate roles of MEL responses. is involved owing to influence regulation. promotes root elongation lateral formation enhances photosynthesis, thereby promoting overall productivity. Additionally, implicated rhythm plants, affecting key physiological processes that patterns. also exhibits antioxidant properties scavenges reactive oxygen species, mitigating oxidative stress. Furthermore, it activates pathways against various biotic stressors. production secondary metabolites contribute resistance environmental changes. MEL’s ability modulate response abiotic stresses been extensively studied. It regulates stomatal closure, conserves water, stress tolerance by activating stress-responsive genes modulating signaling pathways. Moreover, nitric oxide cooperate responses, defense, growth. Understanding mechanisms underlying actions plants will provide new insights into development innovative strategies enhancing crop productivity, improving tolerance, combating diseases. Further this area deepen our knowledge potential applications sustainable agriculture.
Language: Английский
Citations
4
Plant Physiology and Biochemistry, Journal Year: 2025, Volume and Issue: 221, P. 109635 - 109635
Published: Feb. 10, 2025
Melatonin, a versatile biomolecule, profoundly influences plant growth and resilience through its intricate regulation of metabolic pathways, circadian rhythms, cellular processes. The current study elucidates melatonin's concentration-dependent biphasic effects on dynamics in Arabidopsis thaliana Brassica nigra. While 50 μM melatonin optimized biomass accumulation root elongation, higher concentrations (100 μM) elicited stress responses, underscoring dual role as promoter modulator. Melatonin extended photosynthetic efficiency by modulating chlorophyll carotenoid synthesis diurnally, offering protection against photodamage. Divergent responses between the two species, driven species-specific reprogramming, were evident pigment biosynthesis antioxidant pathways. B. nigra displayed robust activation flavonoid phenylpropanoid cytokinin signaling, enhanced oxidative defenses, contrasting with A. thaliana, where suppressed precursors activation. Metabolomic analysis revealed orchestration hormonal crosstalk, involving auxins, gibberellins, jasmonates, to fine-tune adaptation. Stomatal cell wall fortification highlighted optimizing water-use structural under abiotic stress. Cytogenetic studies confirmed safeguarding genomic integrity, regulating chromatin remodeling, promoting DNA repair mechanisms, demonstrating adaptive strategies Moreover, influenced critical including polyamine biosynthesis, sulfur metabolism, nucleotide regulation, emphasizing multifaceted impact homeostasis. These findings position cornerstone molecule biotechnology, potential applications enhancing crop productivity fluctuating environmental conditions.
Language: Английский
Citations
0
The Plant Journal, Journal Year: 2025, Volume and Issue: 121(6)
Published: March 1, 2025
SUMMARY Melatonin significantly influences the regulation of plant growth, development, and stress tolerance. However, regulatory mechanisms underlying melatonin accumulation for drought tolerance in citrus are not fully understood. In this study, we first demonstrated that application exogenous resulted better by reducing water loss maintaining redox homeostasis. Genome‐wide analysis revealed presence 96 genes involved biosynthesis trifoliate orange ( Poncirus trifoliata L., also known as Citrus L.). Seven caffeic acid‐O‐methyltransferases (COMT) were detected, among which PtCOMT5 was most substantially induced predominantly expressed roots leaves. Overexpression led to enhanced promoting root whereas CRISPR‐Cas9‐mediated mutation opposite phenotype. Yeast one‐hybrid screening protein‐DNA interaction assays confirmed transcription factor PtbHLH28 acts a transcriptional activator through interacting with gene promoter. addition, found be positively regulated PtABF4, core member ABA signaling pathway. PtABF4 function regulating ‐mediated synthesis development. Overall, study elucidates crucial role molecular module composed PtABF4‐PtbHLH28‐ modulation development citrus. Our findings shed light on plants exposed gain new insight into network associated response.
Language: Английский
Citations
0
Food Science & Nutrition, Journal Year: 2025, Volume and Issue: 13(4)
Published: April 1, 2025
ABSTRACT Melatonin is recognized as a crucial plant hormone due to its significant role in development, growth promotion, enhancing antioxidant activity, and stress resistance. Soybean one of the most important crops globally. To date, three SNAT genes have been cloned Arabidopsis rice; however, only gene, GmSNAT1 , has characterized soybeans. In this study, members GmGNAT family were identified through genome‐wide analysis. Two candidate GmSNAT ( GmSNAT3.1 GmSNAT3.2 ) selected based on phylogenetic tree analysis exhibited highly similar protein tertiary structures ZmSNAT3. Furthermore, functionality these two was confirmed for synthesizing N‐acetylserotonin or melatonin using serotonin 5‐methoxytryptamine substrates vitro. RNA‐seq data indicated that transcription levels downregulated under conditions dehydration salt stress. Additionally, SNAT3 from other leguminous plants homology with genes. This study elucidates soybeans establishes foundation further exploration into physiological functions within legume plants.
Language: Английский
Citations
0
Journal of Applied Genetics, Journal Year: 2025, Volume and Issue: unknown
Published: April 30, 2025
Language: Английский
Citations
0
Plant Communications, Journal Year: 2023, Volume and Issue: 4(6), P. 100674 - 100674
Published: Aug. 19, 2023
Melatonin (Mel) has previously been reported to effectively alleviate nitrogen-limitation (N-L) stress and thus increase nitrogen-use efficiency (NUE) in several plants, but the underlying mechanism remains obscure. Here, we revealed that OsbZIP79 (BASIC LEUCINE ZIPPER 79) is transcriptionally activated under N-L conditions, its expression further enhanced by exogenous Mel. By combined use of omics, genetics, biological techniques, OsbZIP79-OsABI5 (ABSCISIC ACID INSENSITIVE 5) module stimulated regulation reactive oxygen species (ROS) homeostasis uptake metabolism nitrogen conditions indoor limitation (1/16 normal level). transcription OsABI5, OsABI5 then bound promoters target genes, including genes involved ROS metabolism, activating their transcription. This was also indispensable for upregulation other abscisic acid catabolism, uptake, assimilation Mel treatment, although these were not directly transactivated OsABI5. Field experiments demonstrated significantly improved rice growth low (L-N, half level) same study. application produced a 28.6% yield L-N similar increases NUE. Also, two OsbZIP79-overexpression lines grown field plots had higher NUE (+13.7% +21.2%) than wild types. Together, our data show an regulates response N insufficiency (N or N), which important increasing production.
Language: Английский
Citations
10
Plants, Journal Year: 2024, Volume and Issue: 13(13), P. 1763 - 1763
Published: June 26, 2024
Both melatonin and hydrogen sulfide (H2S) mitigate chromium (Cr) toxicity in plants, but the specific interaction between H2S Cr detoxification remains unclear. In this study, was elucidated by measuring cell wall polysaccharide metabolism antioxidant enzyme activity maize. The findings revealed that exposure to stress (100 μM K2Cr2O7) resulted upregulation of L-/D-cysteine desulfhydrase (LCD/DCD) gene expression, leading a 77.8% 27.3% increase endogenous levels maize leaves roots, respectively. Similarly, system is activated response stress. We found had significant impact on relative expression LCD/DCD, 103.3% 116.7% contrast, NaHS minimal effects mRNA serotonin-Nacetyltransferase (SNAT) levels. production induced accompanied an tolerance, as evidenced elevated content, increased pectin methylesterase activity, improved activity. scavenging decreases melatonin-induced while inhibitor synthesis, p-chlorophenylalanine (p-CPA), has H2S-induced tolerance. conclusion, our suggest serves downstream signaling molecule involved tolerance
Language: Английский
Citations
3
BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)
Published: March 7, 2025
Melatonin plays a pivotal role in alleviating abiotic stresses, yet its biosynthesis regulation crops, particularly wheat, remains unclear. This study explores regulatory components of melatonin under drought stress using bioinformatic, physiochemical, and molecular approaches contrasting wheat genotypes. Bioinformatic analysis identified SNAT, key gene, 88 transcription factors (TFs) from 26 families as potential regulators. The network for SNAT highlighted CYTOKININ-RESPONSIVE GATA FACTOR 1 (CGA1) significant TF. Under stress, genotypes exhibited distinct CGA1-SNAT module expression, cytokinin levels, photosynthetic activity, oxidative damage. Cytokinin treatments regulated the module, altering content, SPAD values, chloroplast numbers, drought-susceptible uncovers interaction with pathway regulating during stress. These findings enhance our understanding mechanisms underpinning tolerance offer promising targets genetic biochemical interventions to improve crop resilience.
Language: Английский
Citations
0
International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(7), P. 6134 - 6134
Published: March 24, 2023
Melatonin is among one of the promising agents able to protect agricultural plants from adverse action different stressors, including salinity. We aimed investigate effects melatonin priming (0.1, 1.0 and 10 µM) on salt-stressed potato (125 mM NaCl), by studying growth parameters, photochemical activity photosystem II, water status, ion content antioxidant system activity. as a pleiotropic signaling molecule was found decrease negative effect salt stress stolon formation, tissue status without significant expression Na+/H+-antiporter genes localized vacuolar (NHX1 NHX3) plasma membrane (SOS1). effectively decreases accumulation lipid peroxidation products in leaves whole range concentrations studied. A melatonin-induced dose-dependent increase Fv/Fm together with uncontrolled non-photochemical dissipation Y(NO) also indicates decreased oxidative damage. The observed protective ability unlikely due its influence enzymes, since neither SOD nor peroxidase were activated melatonin. exerted positive water-soluble low-molecular-weight antioxidants, proline flavonoids, which could aid decreasing stress. most consistent carotenoids, are well-known lipophilic antioxidants playing an important role protection photosynthesis Finally, it possible that accumulated during pretreatment exert direct antioxidative ROS scavenging molecules.
Language: Английский
Citations
8