Characterization of activating cis‐regulatory elements from the histone genes of Chlamydomonas reinhardtii DOI Creative Commons
Yuliia Lihanova, Raimund Nagel, Torsten Jakob

и другие.

The Plant Journal, Год журнала: 2024, Номер 119(1), С. 525 - 539

Опубликована: Май 1, 2024

SUMMARY Regulation of gene expression in eukaryotes is controlled by cis ‐regulatory modules (CRMs). A major class CRMs are enhancers which composed activating elements (CREs) responsible for upregulating transcription. To date, most and CREs have been studied angiosperms; contrast, our knowledge about these key regulators green algae limited. In this study, we aimed at characterizing putative CREs/CRMs from the histone genes unicellular model alga Chlamydomonas reinhardtii . test activity four candidates, reporter constructs consisting a tetramerized CRE, an established promoter, mCerulean3 fluorescent protein were incorporated into nuclear genome C. , their was quantified flow cytometry. Two tested E upstr hist cons significantly upregulated characterized detail. originates highly expressed orientation‐independent CRE capable both RBCS2 β2‐tubulin promoters. CRM angiosperms, upregulates promoter over distance least 1.5 kb. The octamer motif present identified related incerta schloesseri Edaphochlamys debaryana demonstrating its high evolutionary conservation. results investigation expand regulation algae. Furthermore, can be applied as valuable genetic tools.

Язык: Английский

Exchange or Eliminate: The Secrets of Algal-Bacterial Relationships DOI Creative Commons
Bertille Burgunter‐Delamare, Prateek Shetty, Trang Vuong

и другие.

Plants, Год журнала: 2024, Номер 13(6), С. 829 - 829

Опубликована: Март 13, 2024

Algae and bacteria have co-occurred coevolved in common habitats for hundreds of millions years, fostering specific associations interactions such as mutualism or antagonism. These are shaped through exchanges primary secondary metabolites provided by one the partners. Metabolites, N-sources vitamins, can be beneficial to partner they may assimilated chemotaxis towards producing these metabolites. Other metabolites, especially many natural products synthesized bacteria, act toxins damage kill partner. For instance, green microalga Chlamydomonas reinhardtii establishes a mutualistic partnership with Methylobacterium, stark contrast its antagonistic relationship toxin Pseudomonas protegens. In other cases, coccolithophore haptophyte alga Phaeobacter bacterium, same bacterium even subject both processes, depending on secreted bacterial algal Some also influence morphology micronutrients, is observed some macroalgae. This review focuses algal-bacterial micro- macroalgal models from marine, freshwater, terrestrial environments summarizes advances field. It highlights effects temperature it presently known.

Язык: Английский

Процитировано

11
Extensive remodeling during Chlamydomonas reinhardtii zygote maturation leads to highly resistant zygospores DOI Creative Commons

Martim Cardador,

Stephanie Krüger, Susanne Dunker

и другие.

The Plant Journal, Год журнала: 2025, Номер 121(3)

Опубликована: Фев. 1, 2025

SUMMARY The unicellular soil alga Chlamydomonas reinhardtii forms diploid zygotes during its sexual cycle. process of a zygote maturing into highly resistant zygospore remains poorly understood despite importance for survival under adverse environmental conditions. Here we describe the detailed timeline morphological and physiological changes maturation in darkness on ammonium‐free Tris‐acetate‐phosphate agar plates. formation multilayered cell wall is primarily responsible increase size first few days after formation. Desiccation freezing tolerance also develop period 3–7 days. Photosynthetic respiratory activity decrease to reach minimal levels 7–10 days, accompanied by partial dedifferentiation chloroplast that includes chlorophyll degradation followed possible disappearance pyrenoid. In contrast decreasing concentrations most carotenoids formation, ketocarotenoids can be detected 3 their accumulation completed 10 Furthermore, degrades large proportion starch enriches oligosaccharides may serve as osmoprotectants. storage lipid triacylglycerol accumulated at expense thylakoid membrane lipids, which mirrors conversion metabolically active dormant spore metabolic level. Taken together, multifaceted yields mature zygospores ~ weeks. This work sheds light complete time course remodeling photosynthetically eukaryotic dormant, spore.

Язык: Английский

Процитировано

2
A protein blueprint of the diatom CO2-fixing organelle DOI Creative Commons
Onyou Nam,

Sabina Musiał,

Manon Demulder

и другие.

Cell, Год журнала: 2024, Номер unknown

Опубликована: Окт. 1, 2024

Язык: Английский

Процитировано

8
Key Proteomics Tools for Fundamental and Applied Microalgal Research DOI Creative Commons
Maxence Plouviez, Éric Dubreucq

Proteomes, Год журнала: 2024, Номер 12(2), С. 13 - 13

Опубликована: Апрель 4, 2024

Microscopic, photosynthetic prokaryotes and eukaryotes, collectively referred to as microalgae, are widely studied improve our understanding of key metabolic pathways (e.g., photosynthesis) for the development biotechnological applications. Omics technologies, which now common tools in biological research, have been shown be critical microalgal research. In past decade, significant technological advancements allowed omics technologies become more affordable efficient, with huge datasets being generated. particular, where studies focused on a single or few proteins decades ago, it is possible study whole proteome microalgae. The mass spectrometry-based methods has provided this leap forward high-throughput identification quantification proteins. This review specifically provides an overview use proteomics fundamental applied lipid production biofuel) presents future research directions field.

Язык: Английский

Процитировано

5
A novel MoClo-mediated intron insertion system facilitates enhanced transgene expression in Chlamydomonas reinhardtii DOI Creative Commons
Moritz Aschern,

Jochem Braad,

Alfonsina Milito

и другие.

Frontiers in Plant Science, Год журнала: 2025, Номер 16

Опубликована: Март 7, 2025

The Chlamydomonas Modular Cloning (MoClo) toolkit allows for straightforward and flexible construction of genetic modules gene expression in the microalgal model species, fostering developments algal biotechnology. Efficiently expressing transgenes from nuclear genome C. reinhardtii requires proper insertion introns throughout respective gene, as it can substantially enhance expression. To facilitate synthetic biology approaches this microalga, we developed a novel strategy intron into DNA fragments. Our method aligns with current MoClo standards, its feasibility is demonstrated by assembling genes various lengths successfully them . Examples include enhanced NanoLuc increased numbers, fungal luciferase enabling bioluminescence , tryptophan decarboxylase.

Язык: Английский

Процитировано

0
Cross‐Kingdom Genomic Conservation of Putative Human Sleep‐Related Genes: Phylogenomic Evidence From Chlamydomonas reinhardtii DOI
Seithikurippu R. Pandi‐Perumal, Konda Mani Saravanan, Sayan Paul

и другие.

Journal of Cellular Biochemistry, Год журнала: 2025, Номер 126(4)

Опубликована: Март 31, 2025

ABSTRACT Sleep is a widespread and evolutionarily conserved process observed in diverse organisms, from jellyfish to mammals, hinting at its origin as life‐supporting mechanism over 500 million years ago. Although fundamental purpose mechanisms remain unclear, sleep's evolution adaptive significance continue be debated. This study explores the evolutionary origins of sleep using Chlamydomonas reinhardtii model organism, identifying 112 putative sleep‐related genes across species highlighting conservation sleep‐regulatory pathways. Additionally, discovering uncharacterized proteins with high sequence similarity significant e‐values suggests unexplored roles regulation, underscoring potential C. reveal new insights into molecular basis sleep. provides foundation for previously unknown sleep‐associated proteins, particularly within single‐celled which may offer novel perspectives on biological role The demonstrates that phylogenomic analysis organisms can expand our understanding trajectory function, paving way further research biology health implications. Overall, functions higher animal phyla originated primordial activities, demonstrating an continuum wherein more specialized tasks were integrated essential restorative properties.

Язык: Английский

Процитировано

0
A Protein Blueprint of the Diatom CO2-Fixing Organelle DOI Creative Commons
Onyou Nam, Sabina Musiał, Manon Demulder

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Окт. 26, 2023

Abstract Diatoms are central to the global carbon cycle. At heart of diatom fixation is an overlooked organelle called pyrenoid, where concentrated CO 2 delivered densely packed Rubisco. Diatom pyrenoids fix approximately one-fifth , but virtually nothing known about this in diatoms. Using large-scale fluorescence protein tagging and affinity purification-mass spectrometry, we generate a high-confidence spatially-defined protein-protein interaction network for pyrenoid. Within our pyrenoid 10 proteins with no function. We show that six these form static shell encapsulating Rubisco matrix critical structural integrity, shape, Although not conserved at sequence level, shares some architectural similarities prokaryotic carboxysomes. Collectively, results support convergent evolution across two main plastid lineages uncover major functional component fixation.

Язык: Английский

Процитировано

4
Characterization of activating cis‐regulatory elements from the histone genes of Chlamydomonas reinhardtii DOI Creative Commons
Yuliia Lihanova, Raimund Nagel, Torsten Jakob

и другие.

The Plant Journal, Год журнала: 2024, Номер 119(1), С. 525 - 539

Опубликована: Май 1, 2024

SUMMARY Regulation of gene expression in eukaryotes is controlled by cis ‐regulatory modules (CRMs). A major class CRMs are enhancers which composed activating elements (CREs) responsible for upregulating transcription. To date, most and CREs have been studied angiosperms; contrast, our knowledge about these key regulators green algae limited. In this study, we aimed at characterizing putative CREs/CRMs from the histone genes unicellular model alga Chlamydomonas reinhardtii . test activity four candidates, reporter constructs consisting a tetramerized CRE, an established promoter, mCerulean3 fluorescent protein were incorporated into nuclear genome C. , their was quantified flow cytometry. Two tested E upstr hist cons significantly upregulated characterized detail. originates highly expressed orientation‐independent CRE capable both RBCS2 β2‐tubulin promoters. CRM angiosperms, upregulates promoter over distance least 1.5 kb. The octamer motif present identified related incerta schloesseri Edaphochlamys debaryana demonstrating its high evolutionary conservation. results investigation expand regulation algae. Furthermore, can be applied as valuable genetic tools.

Язык: Английский

Процитировано

1