Paper electrochemical immunosensor for the rapid screening of Galectin-3 patients with heart failure

Talanta, Год журнала: 2024, Номер 274, С. 126012 - 126012

Опубликована: Март 29, 2024

A paper electrochemical immunosensor for the combined binding and quantification of heart failure (HF) biomarker Galectin-3 has been developed. The simple design new sensor is comprised material that decorated with gold nanostructures, to maximize its electroactive surface area, functionalized target-specific recognition molecules selectively bind protein from aqueous solutions. caused blockage electron flow surface, thus causing oxidation potential shift corresponding current reduce quantitatively increase in concentration within working range 0.5ng/mL-8ng/mL (LOQ-0.5 ng/mL). This novel was able quantify saliva samples HF patients healthy controls 20 min good reproducibility (RSD = 3.64%), without need complex sample processing steps. measurements patient were cross validated by ELISA where percent agreement between two methods found be 92.7% 7.20%). Therefore, a strong rapid cost-effective screening Galectin 3 at points care, supporting timely diagnosis failure.

Язык: Английский

---

Clinical Periodontal Evaluation and Assessment of Dipeptidyl-Peptidase-4 and Galectin-3 in Gingival Crevicular Fluid of Periodontitis Patients with Heart Failure and Diabetes

Journal of Clinical Medicine, Год журнала: 2025, Номер 14(10), С. 3345 - 3345

Опубликована: Май 12, 2025

Background/Objectives: Periodontitis, a prevalent chronic inflammatory disease affecting tooth-supporting structures, has been increasingly linked to systemic conditions such as diabetes mellitus (DM) and cardiovascular diseases (CVDs). This study aimed evaluate the periodontal status levels of dipeptidyl peptidase-4 (DPP-4) galectin-3 (Gal-3) in gingival crevicular fluid (GCF) patients with periodontitis, heart failure (HF), diabetes, exploring their potential biomarkers for association. Methods: A cross-sectional was conducted on 88 categorized into four groups: periodontally systemically healthy (control, C); periodontitis (P); HF (P+HF); HF, (P+HF+D). Periodontal assessed using probing pocket depth (PPD) Gingival Index (GI). GCF samples were collected analyzed DPP-4 Gal-3 ELISA. Statistical analyses performed assess differences between groups correlations. Results: Results indicated significantly higher all test compared controls (p < 0.0001), highest P+HF+D group. Similarly, elevated patients, particularly those there no significant difference P+HF groups. No observed smokers non-smokers regarding these markers. Positive correlations found parameters immunological markers Conclusions: The findings suggest that may serve association being more upregulated failure.

Язык: Английский

---

Multiple Brain Abscesses of Odontogenic Origin. May Oral Microbiota Affect Their Development? A Review of the Current Literature

Applied Sciences, Год журнала: 2021, Номер 11(8), С. 3316 - 3316

Опубликована: Апрель 7, 2021

In the last few years, role of oral microbiota in setting diseases such as caries, periodontal disease, cancer and systemic infections, including rheumatoid arthritis, cardiovascular disease brain abscess (BA), has attracted attention physicians researchers. Approximately 5–7% all BAs have an odontogenic origin, representing important pathological condition with a high morbidity mortality. A systematic search two databases (Pubmed Ovid EMBASE) was performed for studies published up to 5 January 2021, reporting multiple attributed origin. According PRISMA guidelines, we included total 16 papers due infections. The aim this review is investigate treatment modality clinical outcome patients well identify most common pathogens involved status their role, microbiota, onset multidisciplinary approach essential management BAs. Further are required understand better development

Язык: Английский

---

Circulating MicroRNA Expression, Vitamin D, and Hypercortisolism as Predictors of Osteoporosis in Elderly Postmenopausal Women

Disease Markers, Год журнала: 2021, Номер 2021, С. 1 - 12

Опубликована: Дек. 13, 2021

Background. MicroRNAs (miRNA) identified as critical molecular regulators for bone development, function, and modeling/remodeling process could be predictable osteoporotic fractures in postmenopausal elderly women. Aim. The potential diagnostic role of circulating miRNAs, miR-148a miR-122-5p, the pathogenesis osteoporosis its association with markers, hypercortisolism, vitamin D deficiency were explored women osteoporosis. Methods. A total 120 aged 50–80 years old recruited this study, which only 100 eligible amenorrhea at least 12 consecutive months or surgical menopause participated study. Based upon mineral density (BMD) measurements, participants classified according into two groups: normal ( n=45 ; T score ≥-1.0) n=55 T score: ≤-2.5). Circulating estimated by real-time RT-PCR analysis. In addition, colorimetrically ELISA immune assay estimated. miR-148a, cortisol, diagnosis was predicted using analysis respective area under receiver operating characteristic curve (AUC-ROC). Results. expressed level significantly increased miR-122-5p decreased serum patients compared to healthy controls. a significant increase levels s-BAP, CTx decrease T-BMD, OC, s-Ca also identified. All parameters correlated fracture risk parameters; BMD, T lumbar spine (L2-L4). Thus, data showed AUC cut off values (miR-148a; 0.876, miR-122-5p; 0.761) best evaluated clinical that 0.748 cortisol 0.635 values, respectively, reported prediction diagnosis. Conclusion. miRNAs changes both status are associated loss circulation alone combination might considered biomarkers women; however, more studies recommended.

Язык: Английский

---

Efficacy of curcumin gel as an adjunct to scaling and root planing on salivary procalcitonin level in the treatment of patients with chronic periodontitis: a randomized controlled clinical trial

BMC Oral Health, Год журнала: 2023, Номер 23(1)

Опубликована: Ноя. 19, 2023

To evaluate the effect of curcumin gel combined with scaling and root planing (SRP) on salivary procalcitonin in periodontitis treatment.

Язык: Английский

---