Microbial Pathogenesis, Год журнала: 2025, Номер 206, С. 107790 - 107790
Опубликована: Июнь 4, 2025
Язык: Английский
Signal Transduction and Targeted Therapy, Год журнала: 2022, Номер 7(1)
Опубликована: Июнь 25, 2022
Abstract Pseudomonas aeruginosa ( P. ) is a Gram-negative opportunistic pathogen that infects patients with cystic fibrosis, burn wounds, immunodeficiency, chronic obstructive pulmonary disorder (COPD), cancer, and severe infection requiring ventilation, such as COVID-19. also widely-used model bacterium for all biological areas. In addition to continued, intense efforts in understanding bacterial pathogenesis of including virulence factors (LPS, quorum sensing, two-component systems, 6 type secretion outer membrane vesicles (OMVs), CRISPR-Cas their regulation), rapid progress has been made further studying host-pathogen interaction, particularly host immune networks involving autophagy, inflammasome, non-coding RNAs, cGAS, etc . Furthermore, numerous technologic advances, bioinformatics, metabolomics, scRNA-seq, nanoparticles, drug screening, phage therapy, have used improve our defense. Nevertheless, much remains be uncovered about interactions between responses, mechanisms resistance by known or unannotated well mammalian cell signaling pathways. The widespread use antibiotics the slow development effective antimicrobials present daunting challenges necessitate new theoretical practical platforms screen develop mechanism-tested novel drugs treat intractable infections, especially those caused multi-drug strains. Benefited from advancing research tools technology, dissecting this pathogen’s feature entered into molecular mechanistic details dynamic holistic views. Herein, we comprehensively review discuss current status biophysical traits, behaviors, factors, invasive regulators, defense patterns against its infection, which point out directions future investigation add design and/or alternative therapeutics combat clinically significant pathogen.
Язык: Английский
Процитировано
774
Virulence, Год журнала: 2023, Номер 15(1)
Опубликована: Дек. 6, 2023
Acinetobacter baumannii is a common cause of healthcare-associated infections and hospital outbreaks, particularly in intensive care units. Much the success A. relies on its genomic plasticity, which allows rapid adaptation to adversity stress. The capacity acquire novel antibiotic resistance determinants tolerance stresses encountered environment promote spread among patients long-term contamination healthcare setting. This review explores virulence factors physiological traits contributing infection environment. Several cell-associated secreted involved biofilm formation, cell adhesion, invasion, persistence host, as well xeric stress imposed by settings, are illustrated give reasons for pathogen.
Язык: Английский
Процитировано
40
Journal of Hazardous Materials, Год журнала: 2025, Номер 491, С. 137932 - 137932
Опубликована: Март 13, 2025
Язык: Английский
Процитировано
1
Frontiers in Microbiology, Год журнала: 2022, Номер 13
Опубликована: Май 10, 2022
The Fo ATP synthase, the bacterial flagellar motor, and type 9 secretion system (T9SS) are three known proton motive force driven biological rotary motors. In this review, we summarize current information on nuts bolts of T9SS. Torque generation by T9SS, its role in gliding motility bacteria, mechanism via which a T9SS-driven swarm shapes microbiota discussed. knowledge gaps our understanding T9SS machinery outlined.
Язык: Английский
Процитировано
25
Nucleic Acids Research, Год журнала: 2022, Номер 50(17), С. 9675 - 9688
Опубликована: Сен. 12, 2022
Abstract Pseudomonas aeruginosa is an opportunistic pathogen and major cause of hospital-acquired infections. The virulence P. largely determined by its transcriptional regulatory network (TRN). We used 411 transcription profiles from diverse growth conditions to construct a quantitative TRN identifying independently modulated sets genes (called iModulons) their condition-specific activity levels. current study focused on the use iModulons analyze biofilm production antibiotic resistance aeruginosa. Our analysis revealed: (i) 116 iModulons, 81 which show strong association with known regulators; (ii) novel roles regulators in modulating antibiotics efflux pumps; (iii) substrate-efflux pump associations; (iv) differential iModulon response beta-lactam bacteriological physiological media; (v) activation ‘Cell Division’ resulting exposure different (vi) role PprB stress-induced transition planktonic lifestyle. In light these results, construction iModulon-based provides basis for key aspects infection, such as stress responses formation. Taken together, our results offer mechanistic understanding virulence.
Язык: Английский
Процитировано
25
Antibiotics, Год журнала: 2023, Номер 12(2), С. 195 - 195
Опубликована: Янв. 17, 2023
Infections led by Acinetobacter baumannii strains are of great concern in healthcare environments due to the strong ability bacteria spread through different apparatuses and develop drug resistance. Severe diseases can be caused A. critically ill patients, but its biological process mechanism not well understood. Secretion systems have recently been demonstrated involved pathogenic process, five types secretion out currently known six from Gram-negative found baumannii. They promote fitness pathogenesis releasing a variety effectors. Additionally, antibiotic resistance is related some systems. In this review, we describe genetic structural compositions that exist Acinetobacter. addition, function molecular each system summarized explain how they enable these critical pathogens overcome eukaryotic hosts prokaryotic competitors cause diseases.
Язык: Английский
Процитировано
17
Cold Spring Harbor Protocols, Год журнала: 2023, Номер 2024(8), С. pdb.over107754 - pdb.over107754
Опубликована: Июль 17, 2023
The closely related Escherichia coli Ff filamentous phages (f1, fd, and M13) have taken a fantastic journey over the past 60 years, from urban sewerage which they were first isolated, to their use in high-end technologies multiple fields. Their relatively small genome size, high titers, virions that tolerate fusion proteins make Ffs an ideal system for phage display. Folding of fusions oxidizing environment E. periplasm makes platform allows display eukaryotic surface secreted proteins, including antibodies. Resistance broad range pH detergents facilitates affinity screening display, whereas stability at ambient temperature them suitable applications material science nanotechnology. Among phages, only been used technology, because most advanced state knowledge about biology various tools developed as cloning host them. Filamentous thought be rather group, infecting mostly Gram-negative bacteria. A recent discovery more than 10 thousand diverse bacteria archaea, however, opens fascinating prospect novel applications. main aim this review is give detailed biological structural information researchers embarking on projects. secondary discuss yet-unresolved puzzles, well developments biology, viewpoint impact current future
Язык: Английский
Процитировано
14
Nature Communications, Год журнала: 2023, Номер 14(1)
Опубликована: Сен. 13, 2023
Abstract The bacterial T ight ad herence S ecretion ystem (TadSS) assembles surface pili that drive cell adherence, biofilm formation and predation. structure mechanism of the TadSS is mostly unknown. This includes characterisation outer membrane secretin through which pilus channelled recruitment its pilotin. Here we investigate RcpA TadD lipoprotein from Pseudomonas aeruginosa . Light microscopy reveals colocalising with in P. when heterologously expressed Escherichia coli We use cryogenic electron to determine how assemble a channel C13 C14 symmetries. Despite low sequence homology, show shares similar fold type 4 system pilotin PilF. establish C-terminal four residues bind - an interaction essential for formation. binding between appears distinct known secretin-pilotin pairings other secretion systems.
Язык: Английский
Процитировано
13
Journal of Hazardous Materials, Год журнала: 2024, Номер 472, С. 134502 - 134502
Опубликована: Май 3, 2024
Язык: Английский
Процитировано
5
Nature Communications, Год журнала: 2024, Номер 15(1)
Опубликована: Авг. 5, 2024
Abstract The bacterial tight adherence pilus system (TadPS) assembles surface pili essential for adhesion and colonisation in many human pathogens. Pilus dynamics are powered by the ATPase CpaF (TadA), which drives extension retraction cycles Caulobacter crescentus through an unknown mechanism. Here we use cryogenic electron microscopy cell-based light to characterise We show that into a hexamer with C2 symmetry different nucleotide states. Nucleotide cycling occurs intra-subunit clamp-like mechanism promotes sequential conformational changes between subunits. Moreover, comparison of active sites nucleotides bound suggests bidirectional motion. Conserved residues, predicted interact platform proteins CpaG (TadB) CpaH (TadC), mutated vivo establish their role processing. Our findings provide model how TadPS have broad implications other ancient type 4 filament family members power assembly.
Язык: Английский
Процитировано
5