The honeybee microbiota and its impact on health and disease

Nature Reviews Microbiology, Год журнала: 2023, Номер 22(3), С. 122 - 137

Опубликована: Дек. 4, 2023

Язык: Английский

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Human nasal microbiota shifts in healthy and chronic respiratory disease conditions

BMC Microbiology, Год журнала: 2024, Номер 24(1)

Опубликована: Апрель 27, 2024

Abstract Background An increasing number of studies investigate various human microbiotas and their roles in the development diseases, maintenance health states, balanced signaling towards brain. Current data demonstrate that nasal microbiota contains a unique highly variable array commensal bacteria opportunistic pathogens. However, we need to understand how harness current knowledge, enrich with beneficial microorganisms, prevent pathogenic developments. Results In this study, have obtained nasal, nasopharyngeal, bronchoalveolar lavage fluid samples from healthy volunteers patients suffering chronic respiratory tract diseases for full-length 16 S rRNA sequencing analysis using Oxford Nanopore Technologies. Demographic clinical were collected simultaneously. The microbiome 97 people Lithuania inflammatory disease revealed represents upper airways well. Conclusions was enriched pathogens, which could be used as indicators conditions. addition, observed contained several plant- bee-associated species, suggesting possibility enriching via such exposures when needed. These candidate probiotics should investigated modulating effects on airway lung epithelia, immunogenic properties, neurotransmitter content, maintaining nose-brain interrelationships.

Язык: Английский

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Exposure to a fungicide for a field-realistic duration does not alter bumble bee fecal microbiota structure

Applied and Environmental Microbiology, Год журнала: 2024, Номер 90(2)

Опубликована: Янв. 19, 2024

Social bees are frequently exposed to pesticides when foraging on nectar and pollen. Recent research has shown that pesticide exposure not only impacts social bee host health but can also alter the community structure of gut microbiotas. However, most pesticide-bee microbiota interactions been conducted in honey bees; bumble bees, native North American pollinators, have received less attention and, due differences their ecology, may be certain for shorter durations than bees. Here, we examine how fungicide chlorothalonil a short, field-realistic duration alters fecal microbiotas (used as proxy microbiotas) performance. We expose small groups

Язык: Английский

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The gut microbiome differs between hygiene-performing and non-hygiene-performing worker honey bees

Insectes Sociaux, Год журнала: 2025, Номер unknown

Опубликована: Март 6, 2025

Язык: Английский

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Probiotic and postbiotic strategies against foulbrood in honeybees: in vitro and in vivo insights

Опубликована: Апрель 28, 2025

Among the most severe honeybee diseases, American and European foulbroods caused by Paenibacillus larvae Melissococcus plutonius, respectively pose a significant threat to larval health colony survival. Due lack of safe effective treatments, interest is growing in use beneficial bacterial supplements as promising alternative antibiotics. This study evaluated probiotic postbiotic potential selected strains against foulbrood pathogens. An initial screening 25 for anti-foulbrood activity led selection active candidates further investigation. The inhibitory effect their cell-free supernatants (CFS) was assessed mode action investigated. properties were using P. larvae-infected larvae reared under laboratory conditions. Five lactic acid bacteria exhibited strong antagonistic one or both pathogens, CFS displayed effects. Notably, Lactobacillus crispatus Lactiplantibacillus plantarum completely inhibited at dose 12.5% (v/v). Further characterisation these CFS, suggested bacteriostatic effect, mainly attributed organic acids. In vivo assays demonstrated increase survival when supplemented with live L. plantarum, whereas treatments failed rescue infected larvae. These findings highlight postbiotic-based strategies sustainable alternatives managing beekeeping.

Язык: Английский

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Damage to the behavior and physiological functions of Apis mellifera (Hymenoptera: Apidae) by monocrotaline via the modulation of tryptophan metabolism and the corazonin receptor

The Science of The Total Environment, Год журнала: 2024, Номер 952, С. 175931 - 175931

Опубликована: Авг. 30, 2024

Язык: Английский

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Gut microbiota from patients with Parkinson’s disease causes motor deficits in honeybees

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Июль 12, 2024

Objective Parkinson’s disease (PD) is possibly caused by genetic factors, environmental and gut microbiota dysbiosis. This study aims to explore whether the contributes behavior abnormalities of PD. Methods We transplanted from patients with PD or healthy controls (HC) into microbiota-free honeybees. also established two more groups, namely rotenone (ROT) group, in which PD-like symptoms honeybees were induced rotenone, conventional (CV) colonized microbiota. The climbing assay was performed assess motor capabilities Histopathological examination conducted evaluate integrity mucosa. Tyrosine hydroxylase (TH) gene expression levels dopamine (DA) concentrations brain examined. Additionally, metagenomics full-length 16S rRNA analyses identify alterations profiles, both Results Honeybees ROT groups exhibited slower speeds, downregulated TH expression, impaired barriers. Both HC successfully harbored a portion corresponding human donors, differences microbial composition identified. Morganella morganii Erysipelatoclostridium ramosum significantly increased relative abundance while Dorea longicatena , Collinsella aerofaciens Lactococcus garvieae Holdemanella biformis Gemmiger formicilis Blautia obeum showed group. Functional predictions communities group indicated an synthesis hydrogen sulfide methane. Conclusion A novel model patients. linked PD-related behaviors altered microbiota, highlighting potential microbiota-brain axis involvement pathogenesis. previously unrecognized associations pathways related methane have been suggested as contributors development PD, our research further supports this hypothesis.

Язык: Английский

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Interplay between gut symbionts and behavioral variation in social insects

Current Opinion in Insect Science, Год журнала: 2024, Номер 65, С. 101233 - 101233

Опубликована: Июль 15, 2024

Social insects exhibit a high degree of intraspecific behavioral variation. Moreover, they often harbor specialized microbial communities in their gut. Recent studies suggest that these two characteristics social are interlinked: insect phenotypes affect gut microbiota composition, partly through exposure to different environments and diet, return, the has been shown influence behavior. Here, we discuss bidirectional relationship existing between variation composition insects.

Язык: Английский

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Targeted viromes and total metagenomes capture distinct components of bee gut phage communities

Microbiome, Год журнала: 2024, Номер 12(1)

Опубликована: Авг. 23, 2024

Abstract Background Despite being among the most abundant biological entities on earth, bacteriophage (phage) remain an understudied component of host-associated systems. One limitation to studying phage is lack consensus methods for sampling communities. Here, we compare paired total metagenomes and viral size fraction (viromes) as investigating dsDNA communities associated with GI tract two bee species: European honey Apis mellifera eastern bumble Bombus impatiens . Results We find that viromes successfully enriched phage, thereby increasing recovery, but only in bees. In contrast, bees, recovered greater diversity. Across both species, better sampled low occupancy while were biased towards temperate phage. Additionally, many captured by absent altogether from viromes. Comparing between show commercially reared bees are significantly reduced diversity compared likely reflecting differences bacterial titer a broader context, these results highlight complementary nature targeted viromes, especially when applied environments. Conclusions Overall, suggest studies interested assessing should consider using approaches. However, given constraints virome sampling, may serve sample understanding they will preferentially dominant

Язык: Английский

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Phytochemical S -methyl- L -cysteine sulfoxide from Brassicaceae: a key to health or a poison for bees?

Open Biology, Год журнала: 2024, Номер 14(12)

Опубликована: Дек. 1, 2024

Intensive agricultural practices impact the health and nutrition of pollinators like honey bees (

Язык: Английский

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