Future Directions of the Prokaryotic Chromosome Field
Molecular Microbiology,
Год журнала:
2025,
Номер
123(2), С. 89 - 100
Опубликована: Фев. 1, 2025
In
September
2023,
the
Biology
and
Physics
of
Prokaryotic
Chromosomes
meeting
ran
at
Lorentz
Center
in
Leiden,
The
Netherlands.
As
part
workshop,
those
attendance
developed
a
series
discussion
points
centered
around
current
challenges
for
field,
how
these
might
be
addressed,
field
is
likely
to
develop
over
next
10
years.
staff
facilitated
discussions
via
tools
aimed
optimizing
productive
interactions.
This
Perspective
article
summary
reflects
state-of-the-art
field.
It
expected
help
colleagues
advancing
their
own
research
related
prokaryotic
chromosomes
inspiring
novel
interdisciplinary
collaborations.
forward-looking
perspective
highlights
open
questions
driving
builds
on
impressive
recent
progress
areas
as
represented
by
accompanying
reviews,
perspectives,
articles
this
issue.
These
underline
multi-disciplinary
nature
multiple
length
scales
which
chromatin
studied
vitro
highlight
differences
similarities
bacterial
archaeal
chromatin-associated
processes.
Язык: Английский
Subtilosin A production is influenced by surfactin levels in Bacillus subtilis
microLife,
Год журнала:
2025,
Номер
6
Опубликована: Янв. 1, 2025
Abstract
Although
not
essential
for
their
growth,
the
production
of
secondary
metabolites
increases
fitness
producing
microorganisms
in
natural
habitat
by
enhancing
establishment,
competition,
and
nutrient
acquisition.
The
Gram-positive
soil-dwelling
bacterium,
Bacillus
subtilis,
produces
a
variety
metabolites.
Here,
we
investigated
regulatory
relationship
between
non-ribosomal
peptide
surfactin
sactipeptide
bacteriocin
subtilosin
A.
We
discovered
that
B.
subtilis
mutants
lacking
exhibited
higher
A
compared
to
parental
wild-type
strain.
Additionally,
spatial
visualization
demonstrated
secreted
colony
could
suppress
an
adjacent
mutant
production.
Reporter
assays
were
performed
using
specific
transcriptional
regulators,
which
confirmed
role
ResD
as
activator
encoding
biosynthetic
gene
cluster
(BGC),
while
removal
Rok
AbrB
repressors
increased
expression
BGC,
was
further
enhanced
additional
deletion
surfactin,
suggesting
so-far-unidentified
regulator
might
mediate
influence
on
Our
study
reveals
one
metabolite
another,
highlighting
function
be
more
complex
than
its
other
organisms
interactions
among
also
contribute
ecological
significance.
Язык: Английский
Bacterial epigenetics and its implication for agriculture, probiotics development, and biotechnology design
Biotechnology Advances,
Год журнала:
2024,
Номер
75, С. 108414 - 108414
Опубликована: Окт. 1, 2024
Язык: Английский
Coupling of secondary metabolite production inBacillus subtilis
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 15, 2024
Abstract
Although
not
essential
for
their
growth,
the
production
of
secondary
metabolites
increases
fitness
producing
microorganisms
in
natural
habitat
by
enhancing
establishment,
competition
and
nutrient
acquisition.
The
Gram-positive
soil-dwelling
bacterium,
Bacillus
subtilis
produces
a
variety
metabolites.
Here,
we
investigated
regulatory
relationship
between
non-ribosomal
peptide
surfactin
sactipeptide
bacteriocin
subtilosin
A.
We
discovered
that
B.
mutants
lacking
exhibited
higher
A
compared
to
parental
wild-type
strain.
Additionally,
spatial
visualization
demonstrated
secreted
colony
could
suppress
an
adjacent
mutant
production.
Reporter
assays
were
performed
using
specific
transcriptional
regulators
confirmed
role
ResD
as
activator
encoding
BGC,
while
removal
or
Rok
AbrB
repressors
increased
expression
BGC
was
further
enhanced
additional
deletion
surfactin,
suggesting
so
far
unidentified
regulator
might
mediate
influence
on
Our
study
reveals
one
metabolite
another,
highlighting
function
be
more
complex
than
its
other
organisms
interactions
among
also
contribute
ecological
significance.
Importance
Secondary
play
important
life
facilitating
environment,
including
competing
against
microorganisms,
interacting
with
host
allowing
expansion
environment.
However,
cue
molecules
influencing
gene
within
species.
describe
non-ribosomally
synthesized
repress
ribosomally
post
translationally
modified
peptide,
,
revealing
interaction
two
potentially
biocontrol
efficiency
strain
depends
these
plant
pathogen
microorganisms.
Язык: Английский
Circular oligomeric particles formed by Ros/MucR family members mediate DNA organization in α-proteobacteria
Nucleic Acids Research,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 26, 2024
The
transcriptional
regulator
MucR
from
Brucella
species
controls
the
expression
of
many
genes,
including
those
involved
in
virulence,
by
binding
AT-rich
DNA
regions.
and
its
homologs
belong
to
Ros/MucR
family,
whose
members
occur
α-proteobacteria.
is
a
recent
addition
family
histone-like
nucleoid
structuring
(H-NS)
proteins.
Indeed,
despite
lack
sequence
homology,
bears
functional
similarities
with
H-NS
H-NS-like
proteins,
bacterial
genome
acting
as
global
regulators
transcription.
Here
we
present
an
integrated
cryogenic
electron
microscopy
(cryo-EM),
nuclear
magnetic
resonance,
modeling
biochemical
study
shedding
light
on
architecture
abortus
homolog
Ml5
Mesorhizobium
loti.
We
show
that
fold
circular
quaternary
assembly,
which
allows
it
bridge
condense
sequences.
Our
results
are
novel
type
proteins
and,
based
previous
studies,
provide
model
connecting
structure
transcription
regulation
Язык: Английский