Circular oligomeric particles formed by Ros/MucR family members mediate DNA organization in α-proteobacteria DOI Creative Commons
Antonio Chaves-Sanjuán, Gianluca D’Abrosca, Veronica Russo

et al.

Nucleic Acids Research, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 26, 2024

The transcriptional regulator MucR from Brucella species controls the expression of many genes, including those involved in virulence, by binding AT-rich DNA regions. and its homologs belong to Ros/MucR family, whose members occur α-proteobacteria. is a recent addition family histone-like nucleoid structuring (H-NS) proteins. Indeed, despite lack sequence homology, bears functional similarities with H-NS H-NS-like proteins, bacterial genome acting as global regulators transcription. Here we present an integrated cryogenic electron microscopy (cryo-EM), nuclear magnetic resonance, modeling biochemical study shedding light on architecture abortus homolog Ml5 Mesorhizobium loti. We show that fold circular quaternary assembly, which allows it bridge condense sequences. Our results are novel type proteins and, based previous studies, provide model connecting structure transcription regulation

Language: Английский

Future Directions of the Prokaryotic Chromosome Field DOI
Elio A. Abbondanzieri, Anjana Badrinarayanan, Daniela Barillà

et al.

Molecular Microbiology, Journal Year: 2025, Volume and Issue: 123(2), P. 89 - 100

Published: Feb. 1, 2025

In September 2023, the Biology and Physics of Prokaryotic Chromosomes meeting ran at Lorentz Center in Leiden, The Netherlands. As part workshop, those attendance developed a series discussion points centered around current challenges for field, how these might be addressed, field is likely to develop over next 10 years. staff facilitated discussions via tools aimed optimizing productive interactions. This Perspective article summary reflects state-of-the-art field. It expected help colleagues advancing their own research related prokaryotic chromosomes inspiring novel interdisciplinary collaborations. forward-looking perspective highlights open questions driving builds on impressive recent progress areas as represented by accompanying reviews, perspectives, articles this issue. These underline multi-disciplinary nature multiple length scales which chromatin studied vitro highlight differences similarities bacterial archaeal chromatin-associated processes.

Language: Английский

Citations

1
Subtilosin A production is influenced by surfactin levels in Bacillus subtilis DOI Creative Commons
Caja Dinesen, Manca Vertot, Scott A. Jarmusch

et al.

microLife, Journal Year: 2025, Volume and Issue: 6

Published: Jan. 1, 2025

Abstract Although not essential for their growth, the production of secondary metabolites increases fitness producing microorganisms in natural habitat by enhancing establishment, competition, and nutrient acquisition. The Gram-positive soil-dwelling bacterium, Bacillus subtilis, produces a variety metabolites. Here, we investigated regulatory relationship between non-ribosomal peptide surfactin sactipeptide bacteriocin subtilosin A. We discovered that B. subtilis mutants lacking exhibited higher A compared to parental wild-type strain. Additionally, spatial visualization demonstrated secreted colony could suppress an adjacent mutant production. Reporter assays were performed using specific transcriptional regulators, which confirmed role ResD as activator encoding biosynthetic gene cluster (BGC), while removal Rok AbrB repressors increased expression BGC, was further enhanced additional deletion surfactin, suggesting so-far-unidentified regulator might mediate influence on Our study reveals one metabolite another, highlighting function be more complex than its other organisms interactions among also contribute ecological significance.

Language: Английский

Citations

0
Bacterial epigenetics and its implication for agriculture, probiotics development, and biotechnology design DOI

Tatyana L. Povolotsky,

Hilit Levy Barazany,

Yosi Shacham‐Diamand

et al.

Biotechnology Advances, Journal Year: 2024, Volume and Issue: 75, P. 108414 - 108414

Published: Oct. 1, 2024

Language: Английский

Citations

1
Coupling of secondary metabolite production inBacillus subtilis DOI Creative Commons
Caja Dinesen, Manca Vertot, Scott A. Jarmusch

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 15, 2024

Abstract Although not essential for their growth, the production of secondary metabolites increases fitness producing microorganisms in natural habitat by enhancing establishment, competition and nutrient acquisition. The Gram-positive soil-dwelling bacterium, Bacillus subtilis produces a variety metabolites. Here, we investigated regulatory relationship between non-ribosomal peptide surfactin sactipeptide bacteriocin subtilosin A. We discovered that B. mutants lacking exhibited higher A compared to parental wild-type strain. Additionally, spatial visualization demonstrated secreted colony could suppress an adjacent mutant production. Reporter assays were performed using specific transcriptional regulators confirmed role ResD as activator encoding BGC, while removal or Rok AbrB repressors increased expression BGC was further enhanced additional deletion surfactin, suggesting so far unidentified regulator might mediate influence on Our study reveals one metabolite another, highlighting function be more complex than its other organisms interactions among also contribute ecological significance. Importance Secondary play important life facilitating environment, including competing against microorganisms, interacting with host allowing expansion environment. However, cue molecules influencing gene within species. describe non-ribosomally synthesized repress ribosomally post translationally modified peptide, , revealing interaction two potentially biocontrol efficiency strain depends these plant pathogen microorganisms.

Language: Английский

Citations

0
Circular oligomeric particles formed by Ros/MucR family members mediate DNA organization in α-proteobacteria DOI Creative Commons
Antonio Chaves-Sanjuán, Gianluca D’Abrosca, Veronica Russo

et al.

Nucleic Acids Research, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 26, 2024

The transcriptional regulator MucR from Brucella species controls the expression of many genes, including those involved in virulence, by binding AT-rich DNA regions. and its homologs belong to Ros/MucR family, whose members occur α-proteobacteria. is a recent addition family histone-like nucleoid structuring (H-NS) proteins. Indeed, despite lack sequence homology, bears functional similarities with H-NS H-NS-like proteins, bacterial genome acting as global regulators transcription. Here we present an integrated cryogenic electron microscopy (cryo-EM), nuclear magnetic resonance, modeling biochemical study shedding light on architecture abortus homolog Ml5 Mesorhizobium loti. We show that fold circular quaternary assembly, which allows it bridge condense sequences. Our results are novel type proteins and, based previous studies, provide model connecting structure transcription regulation

Language: Английский

Citations

0