Microbial
infections
have
been
recognized
as
one
of
the
most
serious
threats
to
healthcare
and
agriculture
production,
it
is
still
a
great
challenge
explore
antimicrobial
biomaterials
with
supramolecular
self-assembling
systems.
To
address
this
challenge,
novel
bionanohoops
were
fabricated
via
"graft
from"
reversible
addition–fragmentation
chain
transfer
(RAFT)
host–guest
chemistry.
Admittedly,
controllable
grafting
vanillin-derived
homopolymer
(PVMAx)
from
β-cyclodextrin
(β-CD)
synthesize
β-cyclodextrin-grafted
poly(vanillin
methacrylate)
(β-CD-g-PVMAx,
x
=
5,
35,
103)
was
calculated
1H
NMR
integral
area,
2D
NOESY
demonstrated
that
primary
structured
linear
chains
(β-CD-g-PVMA5)
linked
each
other
by
interactions.
Additionally,
GPC
results
illustrated
secondary
nanohoops
([β-CD-g-PVMA5]y,
y
38
or
364)
self-assembled
in
situ
β-CD-g-PVMA5
through
Compared
stacking
nanorods,
not
only
exhibited
excellent
antibacterial
antifungal
activities
but
also
presented
good
biocompatibility
better
paint
adhesion.
Overall,
we
provided
valuable
strategy
constructs
combining
RAFT
chemistry
for
addressing
microbial
infections.
Molecular Plant Pathology,
Год журнала:
2025,
Номер
26(2)
Опубликована: Фев. 1, 2025
ABSTRACT
Necrotrophic
pathogens
cause
serious
threats
to
agricultural
crops,
and
understanding
the
resistance
genes
their
genetic
networks
is
key
breeding
new
plant
cultivars
with
better
traits.
Although
Alternaria
alternata
causes
black
spot
in
important
leafy
brassica
vegetables,
leads
significant
loss
of
yield
food
quality,
little
known
about
plant–
A.
interactions.
In
this
study,
we
used
a
unique
large
collection
single,
double
triple
mutant
lines
defence
metabolite
regulators
Arabidopsis
explore
how
these
transcription
factors
epistatic
may
influence
infections.
This
identified
nine
novel
20
pairs
interactions
that
modulate
plants'
responses
infection.
We
further
showed
glucosinolate
4‐methoxy‐indol‐3‐ylmethyl
only
consistently
responsive
infection
Col‐0
ecotype.
With
exploration
on
modulating
accumulation
glucosinolates
under
infection,
an
inverted
triangle
regulatory
model
was
proposed
for
at
metabolic
level
phenotypic
level.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(12), С. 6798 - 6798
Опубликована: Июнь 20, 2024
The
necrotrophic
plant
pathogenic
fungus
Botrytis
cinerea
(Pers.,
1794),
the
causative
agent
of
gray
mold
disease,
causes
significant
losses
in
agricultural
production.
Control
this
fungal
pathogen
is
quite
difficult
due
to
its
wide
host
range
and
environmental
persistence.
Currently,
management
disease
still
mainly
based
on
chemicals,
which
can
have
harmful
effects
not
only
environment
human
health
but
also
because
they
favor
development
strains
resistant
fungicides.
flexibility
plasticity
B.
challenging
defense
mechanisms
ability
evolve
strategies
escape
chemicals
require
new
control
for
successful
management.
In
review,
some
aspects
host-pathogen
interactions
from
novel
sustainable
could
be
developed
(e.g.,
signaling
pathways,
molecules
involved
immune
mechanisms,
hormones,
post-transcriptional
gene
silencing)
were
analyzed.
New
biotechnological
tools
use
RNA
interference
(RNAi)
are
emerging
crop
protection
scenario
as
versatile,
sustainable,
effective,
environmentally
friendly
alternatives
chemicals.
RNAi-based
fungicides
expected
approved
soon,
although
will
face
several
challenges
before
reaching
market.
Heliyon,
Год журнала:
2025,
Номер
11(2), С. e42037 - e42037
Опубликована: Янв. 1, 2025
This
study
was
performed
to
identify
Botrytis
species
pathogenic
on
strawberry
and
investigate
effect
of
Ferula
gummosa
essential
oil
(EO)
against
spp.
The
infected
plant
samples
were
collected
in
Khorasan
Razavi
province
from
fruits
with
gray
mold
disease
symptoms.
Following
purification
the
fungi,
54
isolates,
53
isolates
identified
as
B.
cinerea
1
isolate
belonged
pelargonii
based
morphological
molecular
(ITS
RPB2
sequences)
identification.
EO
obtained
F.
at
125,
250,
500,
750
1000
μg
mL-1
concentrations
showed
significant
antifungal
mycelial
growth
a
dose
dependent
manner.
Also,
all
tested
strongly
inhibited
spore
germination
pelargonii.
Treatment
EC50
concentration
significantly
reduced
sclerotia
production
Light
electron
microscopy
observations
that
caused
changes
fungal
structures.
activity
cell
wall
degrading
enzymes,
such
cellulase
pectinase
produced
by
both
species.
A
total
22
compounds
gas
chromatography-mass
spectrometry.
major
β-Pinene
(%
37.7),
γ-Terpinene
21)
a-pinene
12).
Moreover,
considerably
severity
infection
According
results
this
study,
inhibitory
impressive
controlling
postharvest
disease.
is
first
report
ability
disease,
which
can
be
suggested
preserver
coating
for
extend
their
shelf
life
during
storage
period.
Mycologia,
Год журнала:
2025,
Номер
unknown, С. 1 - 15
Опубликована: Фев. 3, 2025
In
this
study,
Minimedusa
polyspora
and
Chaetomium
globosum
their
metabolites
were
assessed
in
vitro
for
ability
to
inhibit
growth
of
Alternaria
alternata,
Berkeleyomyces
basicola,
Botrytis
cinerea,
gaining
insights
into
biocontrol
mechanisms.
A
dual
culture,
an
assay
volatile
antimicrobial
compounds
effectiveness
(performed
two
different
conditions),
a
culture
filtrate
antifungal
designed
discriminate
the
involved
Moreover,
filtrates
these
strains
fungistatic
fungicidal
activities
(determining
also
minimum
inhibitory
concentration
concentration)
occurrence
siderophores.
The
results
show
that
both
M.
C.
inhibited,
extents,
all
pathogens
plate
assays.
Both
showed
activities,
pointing
release
diffusible
as
mechanism.
Based
on
are
promising
bioprotection
agents
phytopathogens
species
interest
further
studies
aimed
at
validating
potential
vivo
conditions.
Microorganisms,
Год журнала:
2025,
Номер
13(3), С. 483 - 483
Опубликована: Фев. 21, 2025
One
of
the
most
important
crop
pathogens
is
Botrytis
cinerea.
It
overcomes
plant
defenses
using
laccase,
an
enzyme
which
frequently
researched.
Yet
differences
between
strains
regarding
their
laccase
activity
poorly
understood.
The
aim
this
study
was
to
analyze
genes
in
context
regionality,
vintage,
and
strains.
Eight
were
analyzed
whole
genome
sequencing,
assessed.
differentiated
by
SSR-PCR.
We
looked
at
all
14
known
regions
as
well
promoter
terminator
variant
metrics
phylogenetic
trees.
seem
be
correlated
with
regionality
rather
than
activity,
provides
new
understanding
pathogen
adaption
specific
environments.
Some
gene
showed
little
no
evolutionary
change,
while
other
a
great
variety
changes.
This
research
highlights
taking
different
into
context.
provide
fundamental
information
for
further
research.
Further
studies,
especially
on
expression,
could
insightful
potential
infection.
ABSTRACT
This
study
aimed
to
assess
the
diversity,
genetic
structure
and
fungicide
(thiophanate‐methyl
iprodione)
sensitivity
of
Botrytis
cinerea
populations
from
Brazilian
eucalypt
nurseries.
Among
106
isolates
obtained
eight
commercial
nurseries
in
five
states,
77
haplotypes
were
identified.
The
frequency
distribution
transposons
Boty
Flipper
,
mating‐type
alleles
MAT1‐1
MAT1‐2
inter‐simple
sequence
repeat
(ISSR)
marker
analyses
revealed
different
levels
diversity
among
Significant
linkage
disequilibrium
almost
all
subpopulations
indicates
a
predominantly
asexual
reproductive
mode
However,
differed
significantly
expected
1:1
ratio
for
total
population,
as
well
two
evaluated,
demonstrating
potential
recombination.
Isolates
resistant
at
least
one
evaluated
fungicides
detected
Of
96
screened
resistance,
1%
only
thiophanate‐methyl,
7.3%
iprodione
41.6%
both
fungicides.
Analysis
molecular
variance
showed
that
variation
B.
was
more
related
than
states
origin.
Both
hierarchical
cluster
analysis
discriminant
principal
components
identified
four
main
groups
associated
with
fungicide‐resistant
phenotypes.
These
findings
provide
evidence
are
highly
diverse,
structured
exhibit
phenotypic
singularities
should
be
considered
when
designing
disease
management
strategies.
Plants,
Год журнала:
2025,
Номер
14(7), С. 985 - 985
Опубликована: Март 21, 2025
Plant-derived
biopesticides
are
emerging
as
a
promising
and
popular
alternative
for
promoting
cleaner
safer
agricultural
practices.
The
present
work
aims
to
explore
Argyranthemum
frutescens
(Asteraceae)
source
of
botanical
pesticides
validate
this
through
cultivation
process.
To
task,
bioassay-guided
fractionation
the
ethanolic
root
extracts
from
both
wild
cultivated
A.
on
phytopathogenic
fungi
Botrytis
cinerea,
Fusarium
oxysporum,
Alternaria
alternata
was
conducted.
This
approach
led
identification
polyacetylenes
with
higher
potency
than
commercial
fungicides.
Specifically,
compounds
3
(capillin)
5
(frutescinone)
showed
more
90%
growth
inhibition
at
0.05
mg/mL
concentration
B.
while
2
(capillinol)
were
also
active
positive
controls,
Fosbel-Plus
Azoxystrobin,
against
F.
oxysporum.
structures
isolated
(1-6,
9,
10)
alkamides
(7,
8,
11)
determined
spectroscopic
analysis,
absolute
configuration
stereocenter
C1
1,
2,
4
9
by
NMR-spectroscopy
(R)-(-)-α-methoxy-phenylacetic
chiral
derivatizing
agent,
biogenetic
considerations.
Overall,
study
supports
potential
agrochemical
lead
phytopathogens,
validates
viable
biopesticides.
