Scientia Horticulturae, Год журнала: 2024, Номер 329, С. 113034 - 113034
Опубликована: Фев. 24, 2024
Язык: Английский
Plants, Год журнала: 2022, Номер 11(12), С. 1602 - 1602
Опубликована: Июнь 18, 2022
Ascorbic acid, also known as vitamin C, is a vital antioxidant widely found in plants. Plant fruits are rich ascorbic acid and the primary source of human intake acid. affects fruit ripening stress resistance plays an essential regulatory role development postharvest storage. The metabolic pathway plants has been extensively studied. accumulation can be effectively regulated by genetic engineering technology. transcription factors, protein interactions, phytohormones, environmental but research on mechanism still relatively weak. This paper systematically reviews regulation metabolism recent decades. It provides theoretical basis for in-depth study critical cultivation
Язык: Английский
Процитировано
81
PLANT PHYSIOLOGY, Год журнала: 2023, Номер 192(2), С. 982 - 999
Опубликована: Фев. 24, 2023
Abstract Cold stress seriously affects plant development, resulting in heavy agricultural losses. L-ascorbic acid (AsA, vitamin C) is an antioxidant implicated abiotic tolerance and metabolism of reactive oxygen species (ROS). Understanding whether how cold elicits AsA biosynthesis to reduce oxidative damage important for developing cold-resistant plants. Here, we show that the accumulation response a common mechanism conserved across kingdom, from single-cell algae angiosperms. We identified basic leucine zipper domain (bZIP) transcription factor (TF) kiwifruit (Actinidia eriantha Benth.), AcePosF21, which was triggered by involved regulation defense responses against stress. AcePosF21 interacted with R2R3-MYB TF AceMYB102 directly bound promoter gene encoding GDP-L-galactose phosphorylase 3 (AceGGP3), key conduit regulating biosynthesis, up-regulate AceGGP3 expression produce more AsA, neutralized excess ROS induced On contrary, VIGS or CRISPR-Cas9-mediated editing decreased content increased generation under Taken together, illustrated model regulatory AcePosF21-mediated stress, provides valuable clues manipulating resistance kiwifruit.
Язык: Английский
Процитировано
54
New Phytologist, Год журнала: 2023, Номер 238(5), С. 2064 - 2079
Опубликована: Фев. 27, 2023
Kiwifruit (Actinidia chinensis) is one of the popular fruits world-wide, and its quality mainly determined by key metabolites (sugars, flavonoids, vitamins). Previous works on kiwifruit are mostly done via a single omics approach or involve only limited metabolites. Consequently, dynamic metabolomes during development ripening underlying regulatory mechanisms poorly understood. In this study, using high-resolution metabolomic transcriptomic analyses, we investigated metabolic landscapes at 11 different developmental stages revealed parallel classification 515 their co-expressed genes into 10 distinct vs gene modules (MM GM). Through integrative bioinformatics coupled with functional genomic assays, constructed global map uncovered essential transcriptional networks for all major changes that occurred throughout growth cycle. Apart from known MM GM such as soluble sugars, identified novel transcription factors regulate accumulation procyanidins, vitamin C, other important Our findings thus shed light network provide valuable resource designed improvement quality.
Язык: Английский
Процитировано
52
Molecular Horticulture, Год журнала: 2025, Номер 5(1)
Опубликована: Янв. 24, 2025
Abstract Kiwifruit is an economically and nutritionally important horticultural fruit crop worldwide. The genomic data of several kiwifruit species have been released, providing unprecedented opportunity for pan-genome analysis to comprehensively investigate the inter- intra-species genetic diversity facilitate utilization breeding. Here, we generated a super using 15 high-quality assemblies eight Actinidia species. For gene-based pan-genome, total 61,465 gene families were identified, softcore dispensable genes enriched in biological processes like response endogenous stimulus, hormone cell wall organization or biogenesis. Then, structural variations (SVs) against A. chinensis ‘Donghong’ identified then used construct graph-based genome. Further population-scale SVs based on resequencing from 112 individuals 20 revealed extensive which probably contributed phenotypic among SV hotspot regions found environmental adaptation. Furthermore, systematically resistance analogs (RGAs) pan-RGA dataset reveal potentially involved disease . pan-genomic obtained here useful evolutionary functional studies , facilitates breeding design.
Язык: Английский
Процитировано
3
Molecular Plant, Год журнала: 2022, Номер 16(2), С. 452 - 470
Опубликована: Дек. 31, 2022
Kiwifruit is a recently domesticated horticultural fruit crop with substantial economic and nutritional value, especially because of the high content vitamin C in its fruit. In this study, we de novo assembled two telomere-to-telomere kiwifruit genomes from Actinidia chinensis var. 'Donghong' (DH) latifolia 'Kuoye' (KY), total lengths 608 327 852 640 561 626 bp for 29 chromosomes, respectively. With burst structural variants involving inversion, translocations, duplications within 8.39 million years, metabolite DH KY exhibited differences saccharides, lignans, vitamins. A regulatory ERF098 transcription factor family has expanded eriantha, both which have ultra-high content. each assembly phased into complete haplotypes, identified allelic variations between sets leading to protein sequence 26 494 27 773 gene loci allele-specific expression 4687 12 238 homozygous pairs. Synchronized metabolome transcriptome changes during development revealed same dynamic patterns levels contents; free fatty acids flavonols accumulated early stages, but sugar substances amino late stages. The AcSWEET9b that exhibits dominance was further positively correlate sucrose Compared wild varieties other species, promoters were selected red-flesh kiwifruits increased content, providing possible explanation on why are sweeter. Collectively, these gap-free provide valuable genetic resource investigating domestication mechanisms genome-based breeding kiwifruit.
Язык: Английский
Процитировано
68
The Plant Cell, Год журнала: 2023, Номер 35(5), С. 1474 - 1495
Опубликована: Фев. 14, 2023
Abstract The major antioxidant L-ascorbic acid (AsA) plays important roles in plant growth, development, and stress responses. However, the importance of AsA concentration regulation metabolism reproduction remain unclear. In Arabidopsis (Arabidopsis thaliana) anthers, tapetum monolayer undergoes cell differentiation to support pollen development. Here, we report that a transcription factor, DEFECTIVE IN TAPETAL DEVELOPMENT AND FUNCTION 1 (TDF1), inhibits tapetal division leading differentiation. We identified SKEWED5-SIMILAR 18 (SKS18) as downstream target TDF1. Enzymatic assays showed SKS18, annotated multicopper oxidase-like protein, has ascorbate oxidase activity, oxidation. also show VITAMIN C DEFECTIVE1 (VTC1), an biosynthetic enzyme, is negatively controlled by TDF1 maintain proper contents. Consistently, either knockout SKS18 or VTC1 overexpression raised concentrations, resulting extra cells, while tdf1 vtc1-3 double mutant mitigated their defective tapetum. observed high concentrations caused lower accumulation reactive oxygen species (ROS) cells. Overexpression ROS scavenging genes restored excess divisions. Thus, our findings demonstrate TDF1-regulated balances through governing homeostasis.
Язык: Английский
Процитировано
33
PLANT PHYSIOLOGY, Год журнала: 2023, Номер 192(3), С. 1684 - 1695
Опубликована: Апрель 19, 2023
Abstract L-Ascorbic acid (AsA) is more commonly known as vitamin C and an indispensable compound for human health. As a major antioxidant, AsA not only maintains redox balance resists biological abiotic stress but also regulates plant growth, induces flowering, delays senescence through complex signal transduction networks. However, content varies greatly in horticultural crops, especially fruit crops. The of the highest species approximately 1,800 times higher than that lowest species. There have been significant advancements understanding accumulation past 20 years. most noteworthy accomplishment was identification critical rate-limiting genes 2 synthesis pathways (L-galactose pathway D-galacturonic pathway) former are GMP, GME, GGP, GPP, gene latter GalUR. Moreover, APX, MDHAR, DHAR regarded key degradation regeneration pathways. Interestingly, some these sensitive to environmental factors, such GGP being induced by light. efficiency enhancing high editing upstream open reading frames (uORF) constructing multi-gene expression vectors. In summary, metabolism has well understood transport mechanism synergistic improvement other traits less known, which will be focus research
Язык: Английский
Процитировано
31
Journal of Experimental Botany, Год журнала: 2024, Номер 75(9), С. 2604 - 2630
Опубликована: Фев. 1, 2024
Abstract Ascorbate (vitamin C) is one of the most abundant primary metabolites in plants. Its complex chemistry enables it to function as an antioxidant, a free radical scavenger, and reductant for iron copper. biosynthesis occurs via mannose/l-galactose pathway green plants, evidence this being major route reviewed. accumulation leaves responsive light, reflecting various roles photoprotection. GDP-l-galactose phosphorylase (GGP) first dedicated step important controlling ascorbate synthesis. expression determined by combination transcription translation. Translation controlled upstream open reading frame (uORF) which blocks translation main GGP-coding sequence, possibly ascorbate-dependent manner. GGP associates with PAS-LOV protein, inhibiting its activity, dissociation induced blue light. While low mutants are susceptible oxidative stress, they grow nearly normally. In contrast, lacking do not unless rescued supplementation. Further research should investigate possible basal functions severely deficient plants involving prevention overoxidation 2-oxoglutarate-dependent dioxygenases mobilization during seed development germination.
Язык: Английский
Процитировано
18
Critical Reviews in Biotechnology, Год журнала: 2024, Номер 44(7), С. 1350 - 1366
Опубликована: Янв. 1, 2024
Plant growth and productivity are continually being challenged by a diverse array of abiotic stresses, including: water scarcity, extreme temperatures, heavy metal exposure, soil salinity. A common theme in these stresses is the overproduction reactive oxygen species (ROS), which disrupts cellular redox homeostasis causing oxidative damage. Ascorbic acid (AsA), commonly known as vitamin C, an essential nutrient for humans, also plays crucial role plant kingdom. AsA synthesized plants through d-mannose/l-galactose pathway that functions powerful antioxidant protects cells from ROS generated during photosynthesis. controls several key physiological processes, photosynthesis, respiration, carbohydrate metabolism, either acting co-factor metabolic enzymes or regulating redox-status. AsA's multi-functionality uniquely positions it to integrate recalibrate redox-responsive transcriptional/metabolic circuits biological accordance developmental environmental cues. In recognition this, we present systematic overview current evidence highlighting central metabolite-switch plants. Further, comprehensive genetic manipulation genes involved metabolism has been provided along with bottlenecks future research directions, could serve framework designing "stress-smart" crops future.
Язык: Английский
Процитировано
11
PLANT PHYSIOLOGY, Год журнала: 2024, Номер 195(3), С. 2053 - 2072
Опубликована: Март 27, 2024
Abstract Carotenoids are major pigments contributing to fruit coloration. We previously reported that the apple (Malus domestica Borkh.) mutant fruits of “Beni Shogun” and “Yanfu 3” show a marked difference in However, regulatory mechanism underlying this phenomenon remains unclear. In study, we determined carotenoid is main factor influencing flesh color. identified an R1-type MYB transcription (TF), MdMYBS1, which was found be highly associated with carotenoids abscisic acid (ABA) contents fruits. Overexpression MdMYBS1 promoted, silencing repressed, β-branch synthesis ABA accumulation. regulates biosynthesis by directly activating biosynthetic genes encoding phytoene synthase (MdPSY2-1) lycopene β-cyclase (MdLCYb). 9-cis-epoxycarotenoid dioxygenase 1 (MdNCED1) contributes biosynthesis, enhances endogenous accumulation MdNCED1 promoter. addition, basic leucine zipper domain TF ABSCISIC ACID-INSENSITIVE5 (MdABI5) as upstream activator promotes Furthermore, MdABI5 promoter activities. Our findings demonstrate MdABI5–MdMYBS1 cascade activated carotenoid-derived coloration apple, providing avenues breeding planting for improvement quality.
Язык: Английский
Процитировано
10