Integration of metabolomics and transcriptomics reveals the regulation mechanism of the phenylpropanoid biosynthesis pathway in insect resistance traits in Solanum habrochaites

Horticulture Research, Год журнала: 2024, Номер 11(2)

Опубликована: Янв. 9, 2024

Abstract Solanum habrochaites (SH), a wild species closely related to ‘Ailsa Craig’ (AC), is an important germplasm resource for modern tomato breeding. Trichomes, developed from epidermal cells, have role in defense against insect attack, and their secretions are of non-negligible value. Here, we found that the glandular heads type VI trichomes were clearly distinguishable between AC SH under cryo-scanning electron microscopy, difference indicating could secrete more anti-insect metabolites than AC. Pest preference experiments showed aphids mites preferred feed near compared with SH. Integration analysis transcriptomics metabolomics data revealed phenylpropanoid biosynthesis pathway was secondary metabolic plants, secreted larger amounts phenylpropanoids flavonoids by upregulating expression relevant genes this pathway, may contribute greater resistance phytophagous insects. Notably, virus-induced silencing Sl4CLL6 not only decreased downstream (SlHCT, SlCAD, SlCHI), but also reduced tomato. These findings provided new genetic resources synthesis compounds breeding S. theoretical basis improvement traits cultivated

Язык: Английский

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Plant secondary metabolites against biotic stresses for sustainable crop protection

Biotechnology Advances, Год журнала: 2025, Номер unknown, С. 108520 - 108520

Опубликована: Янв. 1, 2025

Язык: Английский

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Lignin Metabolism Is Crucial in the Plant Responses to Tambocerus elongatus (Shen) in Camellia sinensis L.

Plants, Год журнала: 2025, Номер 14(2), С. 260 - 260

Опубликована: Янв. 17, 2025

Tambocerus elongatus (Shen) (Hemiptera: Cicadellidae) is a devastating insect pest species of Camellia sinensis, significantly affecting the yield and quality tea. Due to growing concerns over irrational use insecticides associated food safety, it crucial better understand innate resistance mechanism tea trees T. elongatus. This study aims explore responses different levels infestation. We first focused on primary metabolism found that amino acid decreased with increasing infestation, while sugar accumulation showed an opposite trend. Moreover, secondary metabolite analysis significant increase in flavonoid compounds lignin content after Metabolomics revealed decrease proanthocyanidin level anthocyanidin glycosides (anthocyanins their derivatives) infestation also caused abundance non-ester catechins ester catechins. Furthermore, gene expression transcripts genes involved biosynthesis, such as CsCHI, CsF3H, CsF3'H, CsFNS, CsFLS, CsUFGT, were down-regulated, pathway up-regulated by suggesting probably plays pivotal role plant response Analysis related indicates jasmonate (JA) primarily responds leafhopper damage. These findings suggest JA play preferential production saccharides anthocyanin metabolic are critical during this stress response. Further exploration roles tree could provide theoretical basis for understanding defense against

Язык: Английский

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Phytoalexin gene regulation in Arabidopsis thaliana – On the verge of a paradigm shift?

Current Plant Biology, Год журнала: 2024, Номер 39, С. 100367 - 100367

Опубликована: Июнь 30, 2024

Phytoalexins are plant-specialized metabolites that biosynthesized de novo in response to pathogens. Their biosynthesis is highly diverse, with different plant lineages biosynthesizing unique molecules. A common notion individual species produce one type of phytoalexin molecule. Arabidopsis well known an indole alkaloid phytoalexin, named camalexin, as important component its defense against microbial However, studies collectively demonstrate produces a diverse array molecules from branches primary and specialized metabolism. The signaling proteins stimulate synthesis conserved plants, yet each transcription factor they converge upon has been reported regulate the specific class metabolite. We have conducted this review because recent demonstrated homologs factors dissimilar biosynthetic pathways other species. These findings challenge paradigm role regulating Here, we Arabidopsis, them, discoveries on their regulatory mechanisms. discuss crop suggest WRKY33, ERF1, ANAC042, MYB15, MYB72, protein JAZ1, part 'core' network conserved, regulates distinct lineages. Finally, highlight questions raised by that, once solved, will provide major advances our understanding evolution biochemical defenses plants. Recent evidence demonstrates these can be manipulated enhance production pathogen resistance

Язык: Английский

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Designing of future ornamental crops: a biotechnological driven perspective

Horticulture Research, Год журнала: 2023, Номер 10(11)

Опубликована: Сен. 25, 2023

With a basis in human appreciation of beauty and aesthetic values, the new era ornamental crops is based on implementing innovative technologies transforming symbols into tangible assets. Recent advances plant biotechnology have attracted considerable scientific industrial interest, particularly terms modifying desired traits developing future crops. By utilizing omics approaches, genomic data, genetic engineering, gene editing tools, scientists successively explored underlying molecular mechanism potential gene(s) behind trait regulation such as floral induction, architecture, stress resistance, plasticity, adaptation, phytoremediation crop species. These signs progress lay theoretical practical foundation for designing enhancing efficiency plants wide range applications. In this review, we briefly summarized existing literature biotechnological approaches improvement vital plants. The plants, light-emitting biotic/abiotic detectors, pollution abatement, introduction varieties via domestication wild species are also discussed.

Язык: Английский

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CsPAT1, a GRAS transcription factor, promotes lignin accumulation by antagonistic interacting with CsWRKY13 in tea plants

The Plant Journal, Год журнала: 2024, Номер 118(5), С. 1312 - 1326

Опубликована: Фев. 6, 2024

SUMMARY Lignin is an important component of plant cell walls and plays crucial roles in the essential agronomic traits tea quality tenderness. However, molecular mechanisms underlying regulation lignin biosynthesis plants remain unclear. CsWRKY13 acts as a negative regulator plants. In this study, we identified GRAS transcription factor, phytochrome A signal transduction 1 (CsPAT1), that interacts with CsWRKY13. Silencing CsPAT1 expression heterologous overexpression Arabidopsis demonstrated positively regulates accumulation. Further investigation revealed directly binds to promoters CsPAL CsC4H suppresses . indirectly affects promoter activities by interacting CsWRKY13, thereby facilitating Compared alone, co‐expression Oryza sativa significantly increased biosynthesis. Conversely, compared O. reduced These results antagonistic pathway findings improve our understanding provide insights into role factor family

Язык: Английский

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GERDH: an interactive multi‐omics database for cross‐species data mining in horticultural crops

The Plant Journal, Год журнала: 2023, Номер 116(4), С. 1018 - 1029

Опубликована: Июнь 13, 2023

Horticultural plants contribute immensely to the quality of human's life. The rapid development omics studies on horticultural has resulted in large volumes valuable growth- and development-related data. Genes that are essential for growth highly conserved evolution. Cross-species data mining reduces impact species heterogeneity been extensively used gene identification. Owing lack a comprehensive database cross-species using multi-omics from all plant species, current resources this field far satisfactory. Here, we introduce GERDH (https://dphdatabase.com), platform among plants, based 12 961 uniformly processed publicly available libraries more than 150 accessions, including fruits, vegetables ornamental plants. Important genes specific biological process can be obtained by analysis module with interactive web-based visualization. Moreover, is equipped seven online tools, expression, in-species analysis, epigenetic regulation, co-expression, enrichment/pathway phylogenetic analysis. By identified key contributing postharvest storage. expression explored new functions CmEIN3 flower development, which was validated transgenic chrysanthemum We believe will useful resource identification allow big accessible community members.

Язык: Английский

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Genome-wide analysis of the WRKY family in Nicotiana benthamiana reveals key members regulating lignin synthesis and Bemisia tabaci resistance

Industrial Crops and Products, Год журнала: 2024, Номер 222, С. 119655 - 119655

Опубликована: Сен. 14, 2024

Язык: Английский

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NtWRKY28 orchestrates flavonoid and lignin biosynthesis to defense aphid attack in tobacco plants

Plant Physiology and Biochemistry, Год журнала: 2025, Номер 221, С. 109673 - 109673

Опубликована: Фев. 19, 2025

Язык: Английский

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CmHRE2L-CmACS6 transcriptional cascade negatively regulates waterlogging tolerance in Chrysanthemum

Molecular Horticulture, Год журнала: 2025, Номер 5(1)

Опубликована: Март 3, 2025

Abstract The role of ethylene as an initial signaling molecule in waterlogging stress is well-established. However, the complex molecular mechanisms underlying biosynthesis and its functional significance chrysanthemums under conditions have remained unclear. In this study, we observed increase expression 1-aminocyclopropane-1-carboxylate synthase 6 ( CmACS6 ), which encodes a key enzyme responsible for biosynthesis, response to waterlogging. This elevation increases production, induces leaf chlorosis, enhances chrysanthemum's sensitivity stress. Moreover, our analysis upstream regulators revealed that , waterlogging, directly upregulated by CmHRE2-like (Hypoxia Responsive ERF-like, CmHRE2L), factor. Notably, CmHRE2-L binds GCC-like motif promoter region . Genetic validation assays demonstrated CmHRE2L was induced contributed consequently reducing tolerance partially -dependent manner. study identified regulatory module involving governs

Язык: Английский

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