Journal of Integrative Plant Biology,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 25, 2024
ABSTRACT
Wild
species
of
domesticated
crops
provide
valuable
genetic
resources
for
resistance
breeding.
Prunus
davidiana
,
a
wild
relative
peach
with
high
heterozygosity
and
diverse
stress
tolerance,
exhibits
against
aphids.
However,
the
highly
heterozygous
genome
P.
makes
determining
underlying
factors
influencing
traits
challenging.
Here,
we
present
501.7
Mb
haplotype‐resolved
assembly
.
Genomic
comparisons
two
haplotypes
revealed
18,152
structural
variations,
2,699
Pda_hap1‐specific
2,702
Pda_hap2‐specific
genes,
1,118
allele‐specific
expressed
genes.
Genome
composition
indicated
4.1%
was
non‐peach
origin,
out
which
94.5%
derived
from
almond.
Based
on
haplotype
genome,
aphid
quantitative
trait
locus
(QTL)
mapped
at
end
Pda03.
From
QTL,
PdaWRKY4
identified
as
major
dominant
gene,
9‐bp
deletion
in
its
promoter
resistant
phenotype.
Specifically,
regulates
by
promoting
PdaCYP716A1
‐mediated
anti‐aphid
metabolite
betulin
biosynthesis.
Moreover,
employed
design
to
develop
breeding
workflow
rapidly
precisely
producing
aphid‐resistant
peaches.
In
conclusion,
this
study
identifies
novel
gene
provides
insights
into
development
fruit
cultivars.
Journal of Agricultural and Food Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 1, 2025
Sitobion
avenae
is
an
important
pest
that
threatens
the
safety
of
wheat
production
in
China.
However,
resistance
mechanisms
to
S.
are
not
well
understood
at
present.
In
this
study,
we
investigated
interaction
between
and
four
infestation
time
points
(6,
24,
48,
72
hpi)
using
a
high-resolution
series
dual
transcriptomic
analysis.
The
results
showed
plant
hormone
signal
transduction,
phenylpropanoid
biosynthesis,
flavonoid
biosynthesis
pathways
were
significantly
activated
spike
Lunxuan144
during
infestation.
Meanwhile,
functional
analysis
transcriptome
revealed
some
secretory
proteins
participated
wheat-S.
interaction.
This
study
sheds
light
on
arms
race
process
wheat,
laying
foundation
for
green
prevention
providing
theoretical
basis
mining
key
genes
both
avenae.
Journal of Advanced Research,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 1, 2025
Timely
anther
dehiscence
is
a
key
step
for
successful
sexual
reproduction
in
plants.
Secondary
cell
wall
thickening
of
endothecium
vital
process
during
that
provides
an
indispensable
mechanical
force
dehiscence.
Anther
depends
on
lignification,
and
it
timely
sophisticated
regulated
by
phytohormones
transcription
factors.
However,
whether
lignification
occurs
lily
underlying
mechanisms
are
still
largely
unclear.
Our
work
focuses
identifying
the
course
elucidating
molecular
lignification-dependent
lily.
Lignin
fluorescence
analysis
ultraviolet
spectrophotometry
were
employed
to
elucidate
process.
Target
genes
isolated
from
transcriptomic
data
Virus-induced
gene
silencing
(VIGS)
transient
overexpression
anthers
used
analyze
LoMYB26
function.
Yeast
one-hybrid
(Y1H),
electrophoretic
mobility
shift
assay
(EMSA),
dual-luciferase
(LUC)
analyzed
regulatory
mechanisms.
two-hybrid
(Y2H),
luciferase
complementation
imaging
(LCI),
bimolecular
(BiFC)
assays
illustrated
interaction
between
LoJAZ4.
results
showed
occurred
S6-S7
stages
when
had
not
yet
occurred.
The
R2R3-type
MYB
factor,
LoMYB26,
was
found
promote
lignification.
directly
bound
Caffeic
Acid
O-methyltransferase
(LoCOMT)
promoter
activated
its
transcription.
Meanwhile,
interacted
with
jasmonate-ZIM
domain
protein
4
(LoJAZ4),
which
repressed
LoMYB26-mediated
activation
LoCOMT
Additionally,
exogenous
application
methyl-jasmonate
(Me-JA)
induced
promoted
findings
demonstrate
promotes
LoJAZ4,
forming
heterodimer
participates
JA-mediated
This
study
offers
valuable
insights
theoretical
foundation
breeding
anther-indehiscent
Agronomy,
Год журнала:
2023,
Номер
13(11), С. 2819 - 2819
Опубликована: Ноя. 15, 2023
Lignin
is
an
important
secondary
metabolite
that
maintains
the
mechanical
strength
of
horticultural
plants
and
enhances
their
ability
to
respond
external
environmental
changes
such
as
biotic
abiotic
stresses.
However,
excessive
accumulation
lignin
can
lead
lignification
products,
reducing
taste
quality
nutritional
value.
Therefore,
content
products
needs
be
controlled
at
a
reasonable
level,
studying
regulating
metabolism
very
meaningful
work.
This
article
focuses
on
synthesis,
accumulation,
regulation
in
crops
recent
years,
provides
systematic
analysis
its
molecular
mechanism
application
prospects,
sheds
insights
into
directions
need
further
research
future.
basis
for
proposes
new
ideas
improving
crops.
Black
spot
disease
caused
by
the
necrotrophic
fungus
Alternaria
spp.
is
one
of
most
devastating
diseases
affecting
Chrysanthemum
morifolium.
There
currently
no
effective
way
to
prevent
chrysanthemum
black
spot.We
revealed
that
pre-treatment
leaves
with
methy
jasmonate
(MeJA)
significantly
reduces
their
susceptibility
alternata.
To
understand
how
MeJA
treatment
induces
resistance,
we
monitored
dynamics
metabolites
and
transcriptome
in
after
following
A.
alternata
infection.
JA
signaling
affected
resistance
plants
pathogens
through
cell
wall
modification,
Ca2+
regulation,
reactive
oxygen
species
(ROS)
mitogen-activated
protein
kinase
cascade
hormonal
processes,
accumulation
anti-fungal
anti-oxidant
metabolites.
Furthermore,
expression
genes
associated
these
functions
was
verified
reverse
transcription
quantitative
PCR
transgenic
assays.Our
findings
indicate
could
be
a
potential
orchestrator
broad-spectrum
defense
response
may
help
establish
an
ecologically
friendly
pest
control
strategy
offer
promising
priming
induce
responses
against
The Plant Journal,
Год журнала:
2023,
Номер
116(6), С. 1652 - 1666
Опубликована: Сен. 11, 2023
TEMPRANILLO1
(TEM1)
is
a
transcription
factor
belonging
to
related
ABI3
and
VP1
family,
which
also
known
as
ethylene
response
DNA-binding
1
functions
repressor
of
flowering
in
Arabidopsis.
Here,
putative
homolog
AtTEM1
was
isolated
characterized
from
chrysanthemum,
designated
CmTEM1.
Exogenous
application
ethephon
leads
an
upregulation
the
expression
Knockdown
CmTEM1
promotes
floral
initiation,
while
overexpression
retards
transition.
Further
phenotypic
observations
suggested
that
involves
ethylene-mediated
inhibition
flowering.
Transcriptomic
analysis
established
integrator
CmAFL1,
member
APETALA1/FRUITFULL
subfamily,
downregulated
significantly
CmTEM1-overexpressing
transgenic
plants
compared
with
wild-type
but
verified
be
upregulated
amiR-CmTEM1
lines
by
quantitative
RT-PCR.
In
addition,
capable
binding
promoter
CmAFL1
gene
inhibit
its
transcription.
Moreover,
genetic
evidence
supported
notion
partially
inhibits
transition
targeting
CmAFL1.
conclusion,
these
findings
demonstrate
acts
regulator
delayed
partly
through
interaction
Abstract
Background
The
growth
and
ornamental
value
of
chrysanthemums
are
frequently
hindered
by
aphid
attacks.
ethylene-responsive
factor
(ERF)
gene
family
is
pivotal
in
responding
to
biotic
stress,
including
insect
stress.
However,
date,
little
known
regarding
the
involvement
ERF
transcription
factors
(TFs)
response
chrysanthemum
aphids.
Results
In
present
study,
CmHRE2-like
from
(
Chrysanthemum
morifolium
),
a
activator
that
localizes
mainly
nucleus,
was
cloned.
Expression
induced
infestation.
Overexpression
mediated
its
susceptibility
aphids,
whereas
-SRDX
dominant
repressor
transgenic
plants
enhanced
resistance
suggesting
contributes
flavonoids
-overexpression
were
decreased
29%
28%
two
different
lines,
they
increased
42%
plants.
expression
Chrysanthemum-chalcone-synthase
gene(
CmCHS)
,
chalcone
isomerase
CmCHI)
flavonoid
3′-hydroxylase
CmF3’H
)
downregulated
overexpression
upregulated
plants,
regulates
aphids
partially
through
regulation
biosynthesis.
Conclusion
key
regulating
vulnerability
This
study
offers
fresh
perspectives
on
molecular
mechanisms
chrysanthemum-aphid
interactions
may
bear
practical
significance
for
developing
new
strategies
manage
infestation
chrysanthemums.
