Haplotype‐resolved genome of a heterozygous wild peach reveals the PdaWRKY4‐PdaCYP716A1 module mediates resistance to aphids by regulating betulin biosynthesis DOI Open Access
Junxiu Wang, Yong Li,

Xinwei Wang

и другие.

Journal of Integrative Plant Biology, Год журнала: 2024, Номер unknown

Опубликована: Окт. 25, 2024

ABSTRACT Wild species of domesticated crops provide valuable genetic resources for resistance breeding. Prunus davidiana , a wild relative peach with high heterozygosity and diverse stress tolerance, exhibits against aphids. However, the highly heterozygous genome P. makes determining underlying factors influencing traits challenging. Here, we present 501.7 Mb haplotype‐resolved assembly . Genomic comparisons two haplotypes revealed 18,152 structural variations, 2,699 Pda_hap1‐specific 2,702 Pda_hap2‐specific genes, 1,118 allele‐specific expressed genes. Genome composition indicated 4.1% was non‐peach origin, out which 94.5% derived from almond. Based on haplotype genome, aphid quantitative trait locus (QTL) mapped at end Pda03. From QTL, PdaWRKY4 identified as major dominant gene, 9‐bp deletion in its promoter resistant phenotype. Specifically, regulates by promoting PdaCYP716A1 ‐mediated anti‐aphid metabolite betulin biosynthesis. Moreover, employed design to develop breeding workflow rapidly precisely producing aphid‐resistant peaches. In conclusion, this study identifies novel gene provides insights into development fruit cultivars.

Язык: Английский

A Dual RNA-Seq Analysis Revealed Dynamic Arms Race during the Infestation of Wheat by the English Grain Aphid (Sitobion avenae) DOI
Zhenyu Wang,

Weixi Hao,

Dong‐Hui Geng

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Sitobion avenae is an important pest that threatens the safety of wheat production in China. However, resistance mechanisms to S. are not well understood at present. In this study, we investigated interaction between and four infestation time points (6, 24, 48, 72 hpi) using a high-resolution series dual transcriptomic analysis. The results showed plant hormone signal transduction, phenylpropanoid biosynthesis, flavonoid biosynthesis pathways were significantly activated spike Lunxuan144 during infestation. Meanwhile, functional analysis transcriptome revealed some secretory proteins participated wheat-S. interaction. This study sheds light on arms race process wheat, laying foundation for green prevention providing theoretical basis mining key genes both avenae.

Язык: Английский

Процитировано

0

The LoMYB26/LoJAZ4-LoCOMT module regulates anther dehiscence via Jasmonic acid-mediated endothecium lignification in lily DOI Creative Commons

Qianqian Fang,

Xue Pan, Ze Wu

и другие.

Journal of Advanced Research, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Timely anther dehiscence is a key step for successful sexual reproduction in plants. Secondary cell wall thickening of endothecium vital process during that provides an indispensable mechanical force dehiscence. Anther depends on lignification, and it timely sophisticated regulated by phytohormones transcription factors. However, whether lignification occurs lily underlying mechanisms are still largely unclear. Our work focuses identifying the course elucidating molecular lignification-dependent lily. Lignin fluorescence analysis ultraviolet spectrophotometry were employed to elucidate process. Target genes isolated from transcriptomic data Virus-induced gene silencing (VIGS) transient overexpression anthers used analyze LoMYB26 function. Yeast one-hybrid (Y1H), electrophoretic mobility shift assay (EMSA), dual-luciferase (LUC) analyzed regulatory mechanisms. two-hybrid (Y2H), luciferase complementation imaging (LCI), bimolecular (BiFC) assays illustrated interaction between LoJAZ4. results showed occurred S6-S7 stages when had not yet occurred. The R2R3-type MYB factor, LoMYB26, was found promote lignification. directly bound Caffeic Acid O-methyltransferase (LoCOMT) promoter activated its transcription. Meanwhile, interacted with jasmonate-ZIM domain protein 4 (LoJAZ4), which repressed LoMYB26-mediated activation LoCOMT Additionally, exogenous application methyl-jasmonate (Me-JA) induced promoted findings demonstrate promotes LoJAZ4, forming heterodimer participates JA-mediated This study offers valuable insights theoretical foundation breeding anther-indehiscent

Язык: Английский

Процитировано

0

Influence of phloem lectin CsPP2A1 on aphid development via mediation of phenylpropanoid and flavonoid biosynthesis in cucumber DOI

Li Yapeng,

Yu Zhu,

Dong Lele

и другие.

Pest Management Science, Год журнала: 2025, Номер unknown

Опубликована: Апрель 8, 2025

Abstract BACKGROUND Aphid, Aphis gossypii Glover, is a pest that significantly affects cucumbers ( Cucumis sativus L.). Phloem protein 2 (PP2) conserved phloem lectin. Our previous study showed the expression of CsPP2‐A1 under aphid attack affected accumulation flavonoids and total phenolics in cucumber. The novel mechanism lectin mediating secondary metabolites affecting resistance needs to be investigated. RESULTS weight length aphids on overexpression CsPP2‐A1‐ OE) cucumber plants reduced compared wild‐type (WT). Conversely, RNA interference RNAi) opposite trend. Using metabolomics, small molecular were qualitatively quantitatively assessed WT transgenic after inoculation. resulted up‐regulation differential (DMs) phenylpropanoid biosynthesis, whereas led down‐regulation DMs flavonoid biosynthesis. Concurrently, it was observed CAD activity CsPAL , CsCAD OE‐2 up‐regulated significantly. A significant reduction activities CHI, F3H, CsF3H CsCHS CsFLS CsCCR noted RNAi‐2. CONCLUSION indirectly growth development via mediation indirect effects interaction with offer insights into plant–insect studies. © 2025 Society Chemical Industry.

Язык: Английский

Процитировано

0

Comprehensive transcriptome and metabolome analysis deciphers the mechanism underlying rapid xylem growth in the dominant hybrid poplar QB3 DOI
Weiwei Wang,

Xing Yang,

Senyan Zhang

и другие.

Planta, Год журнала: 2025, Номер 261(6)

Опубликована: Апрель 23, 2025

Язык: Английский

Процитировано

0

More or Less: Recent Advances in Lignin Accumulation and Regulation in Horticultural Crops DOI Creative Commons
Guang‐Long Wang, Jiaqi Wu, Yangyang Chen

и другие.

Agronomy, Год журнала: 2023, Номер 13(11), С. 2819 - 2819

Опубликована: Ноя. 15, 2023

Lignin is an important secondary metabolite that maintains the mechanical strength of horticultural plants and enhances their ability to respond external environmental changes such as biotic abiotic stresses. However, excessive accumulation lignin can lead lignification products, reducing taste quality nutritional value. Therefore, content products needs be controlled at a reasonable level, studying regulating metabolism very meaningful work. This article focuses on synthesis, accumulation, regulation in crops recent years, provides systematic analysis its molecular mechanism application prospects, sheds insights into directions need further research future. basis for proposes new ideas improving crops.

Язык: Английский

Процитировано

8

Genome-wide analysis of the WRKY family in Nicotiana benthamiana reveals key members regulating lignin synthesis and Bemisia tabaci resistance DOI

Shun‐Xia Ji,

Fengbin Zhang,

Hong-Da Song

и другие.

Industrial Crops and Products, Год журнала: 2024, Номер 222, С. 119655 - 119655

Опубликована: Сен. 14, 2024

Язык: Английский

Процитировано

3

Jasmonate signaling drives defense responses against Alternaria alternata in chrysanthemum DOI Creative Commons
Shuhuan Zhang,

Weihao Miao,

Ye Liu

и другие.

BMC Genomics, Год журнала: 2023, Номер 24(1)

Опубликована: Сен. 19, 2023

Black spot disease caused by the necrotrophic fungus Alternaria spp. is one of most devastating diseases affecting Chrysanthemum morifolium. There currently no effective way to prevent chrysanthemum black spot.We revealed that pre-treatment leaves with methy jasmonate (MeJA) significantly reduces their susceptibility alternata. To understand how MeJA treatment induces resistance, we monitored dynamics metabolites and transcriptome in after following A. alternata infection. JA signaling affected resistance plants pathogens through cell wall modification, Ca2+ regulation, reactive oxygen species (ROS) mitogen-activated protein kinase cascade hormonal processes, accumulation anti-fungal anti-oxidant metabolites. Furthermore, expression genes associated these functions was verified reverse transcription quantitative PCR transgenic assays.Our findings indicate could be a potential orchestrator broad-spectrum defense response may help establish an ecologically friendly pest control strategy offer promising priming induce responses against

Язык: Английский

Процитировано

7

Fusion gene 4CL-CCR promotes lignification in tobacco suspension cells DOI
Nan Sun, Jiaqi Hu, Can Li

и другие.

Plant Cell Reports, Год журнала: 2023, Номер 42(5), С. 939 - 952

Опубликована: Март 25, 2023

Язык: Английский

Процитировано

6

The RAV transcription factor TEMPRANILLO1 involved in ethylene‐mediated delay of chrysanthemum flowering DOI Open Access
Hua Cheng,

Qingguo Wang,

Zixin Zhang

и другие.

The Plant Journal, Год журнала: 2023, Номер 116(6), С. 1652 - 1666

Опубликована: Сен. 11, 2023

TEMPRANILLO1 (TEM1) is a transcription factor belonging to related ABI3 and VP1 family, which also known as ethylene response DNA-binding 1 functions repressor of flowering in Arabidopsis. Here, putative homolog AtTEM1 was isolated characterized from chrysanthemum, designated CmTEM1. Exogenous application ethephon leads an upregulation the expression Knockdown CmTEM1 promotes floral initiation, while overexpression retards transition. Further phenotypic observations suggested that involves ethylene-mediated inhibition flowering. Transcriptomic analysis established integrator CmAFL1, member APETALA1/FRUITFULL subfamily, downregulated significantly CmTEM1-overexpressing transgenic plants compared with wild-type but verified be upregulated amiR-CmTEM1 lines by quantitative RT-PCR. In addition, capable binding promoter CmAFL1 gene inhibit its transcription. Moreover, genetic evidence supported notion partially inhibits transition targeting CmAFL1. conclusion, these findings demonstrate acts regulator delayed partly through interaction

Язык: Английский

Процитировано

6

Chrysanthemum (Chrysanthemum morifolium) CmHRE2-like negatively regulates the resistance of chrysanthemum to the aphid (Macrosiphoniella sanborni) DOI Creative Commons
You Wang, Wanwan Zhang,

Chaojun Hong

и другие.

BMC Plant Biology, Год журнала: 2024, Номер 24(1)

Опубликована: Янв. 29, 2024

Abstract Background The growth and ornamental value of chrysanthemums are frequently hindered by aphid attacks. ethylene-responsive factor (ERF) gene family is pivotal in responding to biotic stress, including insect stress. However, date, little known regarding the involvement ERF transcription factors (TFs) response chrysanthemum aphids. Results In present study, CmHRE2-like from ( Chrysanthemum morifolium ), a activator that localizes mainly nucleus, was cloned. Expression induced infestation. Overexpression mediated its susceptibility aphids, whereas -SRDX dominant repressor transgenic plants enhanced resistance suggesting contributes flavonoids -overexpression were decreased 29% 28% two different lines, they increased 42% plants. expression Chrysanthemum-chalcone-synthase gene( CmCHS) , chalcone isomerase CmCHI) flavonoid 3′-hydroxylase CmF3’H ) downregulated overexpression upregulated plants, regulates aphids partially through regulation biosynthesis. Conclusion key regulating vulnerability This study offers fresh perspectives on molecular mechanisms chrysanthemum-aphid interactions may bear practical significance for developing new strategies manage infestation chrysanthemums.

Язык: Английский

Процитировано

2