The fungal effector AaAlta1 inhibits PATHOGENESIS-RELATED PROTEIN10-2-mediated callose deposition and defense responses in apple DOI
Shun Gong, Jinqi Tang, Yi Xiao

и другие.

PLANT PHYSIOLOGY, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 26, 2024

Abstract Apple leaf spot, caused by Alternaria alternata f. sp mali (ALT), poses a substantial threat to the global apple (Malus × domestica Borkh.) industry. Fungal effectors promote pathogen infestation and survival interfering with plant immune responses. In our study, we investigated secretion of effector proteins virulent ALT7 strain. Using mass spectrometry, identified AaAlta1, which belongs Alt 1 protein family (AA1s). Further analysis confirmed that secretes AaAlta1. AaAlta1 knockdown mutants displayed reduced pathogenicity in tissue culture seedlings, while overexpression strains exhibited enhanced compared wild-type immunoprecipitation followed isolated pathogenesis-related 10-2 (PR10-2) as an interaction partner apple. Knockdown showed increased PR10-2-mediated callose deposition apple, critical defense response. The responses substantially their susceptibility infection these mutants. Our findings delineate strategy whereby suppress PR10-2, thereby circumventing system.

Язык: Английский

Plant disease resistance outputs regulated by AP2/ERF transcription factor family DOI Creative Commons

Ning Ma,

Ping Sun, Zhaoyang Li

и другие.

Stress Biology, Год журнала: 2024, Номер 4(1)

Опубликована: Янв. 2, 2024

Abstract Plants have evolved a complex and elaborate signaling network to respond appropriately the pathogen invasion by regulating expression of defensive genes through certain transcription factors. The APETALA2/ethylene response factor (AP2/ERF) family members been determined as key regulators in growth, development, stress responses plants. Moreover, growing body evidence has demonstrated critical roles AP2/ERFs plant disease resistance. In this review, we describe recent advances for function defense against microbial pathogens. We summarize that are involved resistance acting downstream mitogen activated protein kinase (MAPK) cascades, associated with hormonal pathways, biosynthesis secondary metabolites, formation physical barriers an MAPK-dependent or -independent manner. present review provides multidimensional perspective on functions resistance, which will facilitate understanding future investigation immunity.

Язык: Английский

Процитировано

19

Novel Regulators and Their Epistatic Networks in Arabidopsis' Defence Responses to Alternaria alternata Infection DOI Creative Commons
Q. Zeng,

Xifan Liu,

Xuemei Yan

и другие.

Molecular Plant Pathology, Год журнала: 2025, Номер 26(2)

Опубликована: Фев. 1, 2025

ABSTRACT Necrotrophic pathogens cause serious threats to agricultural crops, and understanding the resistance genes their genetic networks is key breeding new plant cultivars with better traits. Although Alternaria alternata causes black spot in important leafy brassica vegetables, leads significant loss of yield food quality, little known about plant– A. interactions. In this study, we used a unique large collection single, double triple mutant lines defence metabolite regulators Arabidopsis explore how these transcription factors epistatic may influence infections. This identified nine novel 20 pairs interactions that modulate plants' responses infection. We further showed glucosinolate 4‐methoxy‐indol‐3‐ylmethyl only consistently responsive infection Col‐0 ecotype. With exploration on modulating accumulation glucosinolates under infection, an inverted triangle regulatory model was proposed for at metabolic level phenotypic level.

Язык: Английский

Процитировано

1

Arabidopsis thaliana accumulates dehydroepiandrosterone after infection with phytopathogenic fungi – effects on plants and fungi. DOI

Ceren Oktay,

Glendis Shiko,

Maximilian Liebl

и другие.

Plant Physiology and Biochemistry, Год журнала: 2025, Номер unknown, С. 109570 - 109570

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0

16S rDNA sequencing combined with metabolomic probes to investigate the effects of Salmonella Pullorum on gut microbes and metabolites in broilers DOI Creative Commons
Jiajun Wu, Ruixiang Xue,

Zhexia Fan

и другие.

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Март 5, 2025

Pullorum disease (PD) caused by Salmonella (SP) results in high mortality chicks and potential carriers adult chickens, negatively affecting growth egg production. This study identified SP infection 100-day-old White Plymouth Rock hens serum plate agglutination fecal anal swab polymerase chain reaction. SP-infected broilers were classified into positive (P) negative (N) groups using hematoxylin-and-eosin staining, metabolome sequencing, 16S rDNA to investigate the effects of on metabolites microorganisms cecum broilers. Groups had different degrees inflammatory cell infiltration cecum, spleen, liver, lung tissues. The diversity bacterial flora P N differed significantly (P < 0.05). o__Lactobacillales o__Verrucomicrobiota higher Group than At genus level, g__Akkermansia was Metabolome sequencing contents screened 77 differential at secondary metabolite level. 11 metabolites, including 2,4-dimethylbenzaldehyde, 3a,6b,7b,12a-tetrahydroxy-5b-cholanoic acid, LysoPG 19:1, differentially expressed A combined analysis cecal content metabolomics 28 genera associated with 38 Specific such as Corynebacterium Roseobacter have particularly prominent metabolites. These findings highlight significant alterations gut microbial composition metabolic functions due infection. taxa this may provide insights underlying mechanisms PD pathogenesis biomarkers for management.

Язык: Английский

Процитировано

0

Alternaria alternata effector AaAlta1 targets CmWD40 and participates in regulating disease resistance in Chrysanthemum morifolium DOI Creative Commons
Shuhuan Zhang, Lina Liu, Wenjie Li

и другие.

PLoS Pathogens, Год журнала: 2025, Номер 21(3), С. e1012942 - e1012942

Опубликована: Март 31, 2025

Black spot diseases caused by the necrotrophic fungal pathogen Alternaria alternata adversely affect growth and yield of many plants worldwide. However, molecular mechanisms underlying virulence pathogenicity A. remain largely unknown. In this study, we report identification a novel effector Alta1, secreted , which not only contributes to its but also triggers cell death defense host plant. The expression Alta1 in Chrysanthemum morifolium activated jasmonic acid (JA) signaling, which, turn, enhanced plant resistance . Moreover, found that targeted WD40-repeat protein chrysanthemum ( Cm WD40) after entering cells. Notably, CmWD40 gene showed rhythmic basal expression, overexpression increased leaves against whereas loss function led decrease resistance. results comparative transcriptomics JA content analyses indicated is possibly involved accumulation signaling JA. transcript levels MYC2 were significantly upregulated lines overexpressing compared with wild type. Further, infection assay revealed positively modulated Alta1-induced response activating transcription. Overall, obtained study demonstrate identified recognized circadian rhythm JA-induced immune enhances disease plants.

Язык: Английский

Процитировано

0

Evaluation of Five Chrysanthemum morifolium Cultivars against Leaf Blight Disease Caused by Alternaria alternata at Rooting and Seedling Growth Stages DOI Creative Commons

Mayada Seliem,

Naglaa Taha,

Nahla I. El-Feky

и другие.

Plants, Год журнала: 2024, Номер 13(2), С. 252 - 252

Опубликована: Янв. 16, 2024

During the winter of 2018, leaf blight on florist’s daisy (Chrysanthemum morifolium L.) was noticed in Egypt. The disease, which identified as caused by Alternaria alternata, widely spread and led to serious damage for exportation sector this crop. Therefore, a study conducted better understand what can be minimize problem future. Isolates were gathered evaluated five chrysanthemum cultivars (i.e., ‘Feeling Green Dark’, ‘Talitha’, ‘Chrystal Regan’, ‘Arctic queen’, ‘Podolsk Purple’) grown greenhouse. objectives isolate identify phytopathogen detect resistant degree these with emphasis early growth stages results showed that Purple’ most cultivar against different isolates during rooting seedling stages. Regan’ very susceptible isolates. In addition, from Dark’ strongest, negatively affected chlorophyll content its fluorescence parameters besides other measured vegetative anatomical features. findings indicated best characters stem leaf, like thickness cuticle cortex, diameter, xylem vessel epidermis well lamina recorded cultivar. This highlighted using right cultivars, cultivated season under Egyptian conditions. These part solution overcome A. alternata growing

Язык: Английский

Процитировано

1

The fungal effector AaAlta1 inhibits PATHOGENESIS-RELATED PROTEIN10-2-mediated callose deposition and defense responses in apple DOI
Shun Gong, Jinqi Tang, Yi Xiao

и другие.

PLANT PHYSIOLOGY, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 26, 2024

Abstract Apple leaf spot, caused by Alternaria alternata f. sp mali (ALT), poses a substantial threat to the global apple (Malus × domestica Borkh.) industry. Fungal effectors promote pathogen infestation and survival interfering with plant immune responses. In our study, we investigated secretion of effector proteins virulent ALT7 strain. Using mass spectrometry, identified AaAlta1, which belongs Alt 1 protein family (AA1s). Further analysis confirmed that secretes AaAlta1. AaAlta1 knockdown mutants displayed reduced pathogenicity in tissue culture seedlings, while overexpression strains exhibited enhanced compared wild-type immunoprecipitation followed isolated pathogenesis-related 10-2 (PR10-2) as an interaction partner apple. Knockdown showed increased PR10-2-mediated callose deposition apple, critical defense response. The responses substantially their susceptibility infection these mutants. Our findings delineate strategy whereby suppress PR10-2, thereby circumventing system.

Язык: Английский

Процитировано

0