MedComm, Год журнала: 2024, Номер 5(10)
Опубликована: Сен. 20, 2024
Radiation-induced tissue injury (RITI) is the most common complication in clinical tumor radiotherapy. Due to heterogeneity response of different tissues radiation (IR), radiotherapy will cause types and degrees RITI, which greatly limits application Efforts are continuously ongoing elucidate molecular mechanism RITI develop corresponding prevention treatment drugs for RITI. Single-cell sequencing (Sc-seq) has emerged as a powerful tool uncovering mechanisms identifying potential targets by enhancing our understanding complex intercellular relationships, facilitating identification novel cell phenotypes, allowing assessment spatiotemporal developmental trajectories. Based on comprehensive review we analyzed regulatory networks combination with Sc-seq summarized targeted intervention pathways therapeutic Deciphering diverse underlying can shed light its pathogenesis unveil new avenues potentially facilitate repair or regeneration currently irreversible Furthermore, discuss how personalized strategies based offer promise mitigating
Язык: Английский
Процитировано
8Navigating the landscape of plant proteomics
Journal of Integrative Plant Biology, Год журнала: 2025, Номер unknown
Опубликована: Янв. 15, 2025
In plants, proteins are fundamental to virtually all biological processes, such as photosynthesis, signal transduction, metabolic regulation, and stress responses. Studying protein distribution, function, modifications, interactions at the cellular tissue levels is critical for unraveling complexities of these pathways. Protein abundance localization highly dynamic vary widely across proteome, presenting a challenge global quantification analysis. Mass spectrometry-based proteomics approaches have proven be powerful tools addressing this complex issue. review, we summarize recent advancements in research their applications plant biology, with an emphasis on current state challenges studying post-translational single-cell proteomics, protein-protein interactions. Additionally, discuss future prospects highlighting potential opportunities that technologies offer advancing biology research.
Язык: Английский
Процитировано
1Trends in Mass Spectrometry-Based Single-Cell Proteomics
Analytical Chemistry, Год журнала: 2025, Номер unknown
Опубликована: Март 16, 2025
InfoMetrics Analytical ChemistryASAPArticle CiteCitationCitation and abstractCitation referencesMore citation options ShareShare onFacebookXWeChatLinkedInRedditEmailBlueskyJump toExpandCollapse ReviewMarch 16, 2025Trends in Mass Spectrometry-Based Single-Cell ProteomicsClick to copy article linkArticle link copied!Ximena Sanchez-AvilaXimena Sanchez-AvilaDepartment of Chemistry Biochemistry, Brigham Young University, Provo, Utah 84602, United StatesMore by Ximena Sanchez-AvilaView BiographyRaphaela M. de OliveiraRaphaela OliveiraDepartment Raphaela OliveiraView BiographySiqi HuangSiqi HuangDepartment Siqi HuangView BiographyChao WangChao WangDepartment Chao WangView Biographyhttps://orcid.org/0009-0008-6197-2985Ryan T. Kelly*Ryan KellyDepartment States*Email: [email protected]More Ryan KellyView Biographyhttps://orcid.org/0000-0002-3339-4443Other Access OptionsAnalytical ChemistryCite this: Anal. Chem. 2025, XXXX, XXX, XXX-XXXClick citationCitation copied!https://pubs.acs.org/doi/10.1021/acs.analchem.5c00661https://doi.org/10.1021/acs.analchem.5c00661Published March 2025 Publication History Received 28 January 2025Accepted February 2025Revised 23 2025Published online 16 2025review-article© American Chemical SocietyRequest reuse permissionsACS Publications© SocietySubjectswhat are subjects Article automatically applied from the ACS Subject Taxonomy describe scientific concepts themes article. Cells Isolation spectrometry Peptides proteins Sample preparation Note: In lieu an abstract, this is article's first page. Read To access article, please review available below. Get instant Purchase for 48 hours. Check out below using your ID or as a guest. Restore my guest Recommended through Your Institution You may have institution. institution does not content. Add change let them know you'd like include access. Through Recommend Name Loading Institutional Login Options... Change Explore subscriptions institutions Log with if you previously purchased it member benefits. hours $48.00 cart Checkout Cited By Click section linkSection copied!This has yet been cited other publications.Download PDF e-AlertsGet e-AlertsAnalytical copied!https://doi.org/10.1021/acs.analchem.5c00661Published 2025© permissionsArticle Views6Altmetric-Citations-Learn about these metrics closeArticle Views COUNTER-compliant sum full text downloads since November 2008 (both HTML) across all individuals. These regularly updated reflect usage leading up last few days.Citations number articles citing calculated Crossref daily. Find more information counts.The Altmetric Attention Score quantitative measure attention that research received online. Clicking on donut icon will load page at altmetric.com additional details score social media presence given how calculated.Recommended Articles
Язык: Английский
Процитировано
1Single-cell proteomics of Arabidopsis leaf mesophyll identifies drought stress-related proteins
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Июль 3, 2024
Abstract The application of single-cell omics tools to biological systems can provide unique insights into diverse cellular populations and their heterogeneous responses internal external perturbations. Thus far, most studies in plant have been limited RNA-sequencing approaches, which only indirect readouts functions. Here, we present a proteomics workflow for cells that integrates tape-sandwich protoplasting, piezoelectric cell sorting, nanoPOTS sample preparation, FAIMS-based MS data acquisition method label-free analysis Arabidopsis leaf mesophyll cells. From single protoplast, over 3,000 proteins were quantified with high precision. is demonstrated identify stress associated changes protein abundance by analyzing >80 protoplasts from well-watered water-deficit stressed plants. Additionally, describe new approach constructing covarying networks at the level demonstrate how covariation reveal previously unrecognized functions while also capturing stress-induced protein-protein dynamics. Highlights This study describes first scProteomics abiotic proteome regulation ∼2800 on average precision using label free leaves revealed known novel involved drought response Single-protoplast water deficit-induced independent
Язык: Английский
Процитировано
4The plant proteome delivers from discovery to innovation
Trends in Plant Science, Год журнала: 2025, Номер unknown
Опубликована: Апрель 1, 2025
Язык: Английский
Процитировано
0Multifunctionality of Vesicle-Associated Membrane Protein 721 and 722 in Plants
Journal of Plant Biology, Год журнала: 2025, Номер unknown
Опубликована: Апрель 17, 2025
Язык: Английский
Процитировано
0Exploring the untapped potential of single‐cell and spatial omics in plant biology
New Phytologist, Год журнала: 2025, Номер unknown
Опубликована: Май 21, 2025
Summary Advances in single‐cell and spatial omics technologies have revolutionised biology by revealing the diverse molecular states of individual cells their organization within tissues. The field plant has widely adopted transcriptome chromatin accessibility profiling transcriptomics, which extend traditional cell genomics analyses provide unique opportunities to reveal cellular dynamics Using these technologies, comprehensive atlases been generated several model species, providing valuable platforms for discovery tool development. Other emerging related omics, such as multiomics, lineage tracing, recording, high‐content genetic chemical perturbation phenotyping, offer immense potential deepening our understanding yet remain underutilised due technical challenges resource availability. Overcoming plant‐specific barriers, wall complexity limited antibody resources, alongside community‐driven efforts developing more complete reference computational tools, will accelerate progress. synergy between technological innovation targeted biological questions is poised drive significant discoveries, advancing science. This review highlights current applications research introduces approaches with transform field.
Язык: Английский
Процитировано
0Pleiotropy, a feature or a bug? Toward co-ordinating plant growth, development, and environmental responses through engineering plant hormone signaling
Current Opinion in Biotechnology, Год журнала: 2024, Номер 88, С. 103151 - 103151
Опубликована: Май 31, 2024
The advent of gene editing technologies such as CRISPR has simplified co-ordinating trait development. However, identifying candidate genes remains a challenge due to complex networks and pathways. These exhibit pleiotropy, complicating the determination specific pathway functions. In this review, we explore how systems biology single-cell sequencing can aid in for specifics plant growth development within temporal tissue contexts. Exploring sequence-function space these modules with synthetic allows us test hypotheses define genotype-phenotype relationships through reductionist approaches. Collectively, techniques hold potential advance breeding genetic engineering strategies while also addressing diversity issues critical adaptation
Язык: Английский
Процитировано
2The whole and its parts: cell-specific functions of brassinosteroids
Trends in Plant Science, Год журнала: 2024, Номер unknown
Опубликована: Ноя. 1, 2024
Язык: Английский
Процитировано
1Proteomics: An Essential Tool to Study Plant-Specialized Metabolism
Biomolecules, Год журнала: 2024, Номер 14(12), С. 1539 - 1539
Опубликована: Ноя. 30, 2024
Plants are a valuable source of specialized metabolites that provide plethora therapeutic applications. They natural defenses plants use to adapt and respond their changing environment. Decoding biosynthetic pathways understanding how plant (SPMs) biotic or abiotic stress will vital knowledge for biology research its application the future sustainable production many SPMs interest. Here, we focus on proteomic approaches strategies help with study plant-specialized metabolism, including the: (i) discovery key enzymes clarification pathways; (ii) interconnection both primary (providers carbon energy SPM production) (secondary) metabolism; (iii) responses stress; (iv) regulatory mechanisms direct pathways. Proteomics, as exemplified in this review by studies performed date, is powerful tool forms part omics-driven research. The proteomes analysis provides an additional unique level information, which absent from any other omics studies. Thus, integrative analysis, considered versus single moves us more closely toward closer interpretation real cellular processes. Finally, work highlights advanced technologies immediate applications field.
Язык: Английский
Процитировано
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