Mini Review: Highlight of Recent Advances and Applications of MALDI Mass Spectrometry Imaging in 2024
Analytical Science Advances,
Год журнала:
2025,
Номер
6(1)
Опубликована: Май 10, 2025
Abstract
Matrix‐assisted
laser
desorption/ionisation
mass
spectrometry
imaging
(MALDI‐MSI)
is
an
emerging
tool
that
allows
visualisation
of
hundreds
analytes
unbiasedly
in
a
single
experiment.
This
paper
highlights
the
adaptations
MALDI‐MSI
different
context
2024,
such
as
clinical
diagnostic,
pharmacology,
forensics
applications,
plant
metabolism
and
biology.
Challenges
advancements
were
also
discussed
regarding
sample
preparation,
instrumentations,
data
analysis,
integration
machine
learning
trend
cell
resolution
multi‐omics.
There
are
still
rooms
for
improvements
sensitivity,
spatial
resolution,
acquisition
algorithm
across
multi‐omics
to
enable
at
subcellular
level.
Язык: Английский
Imaging the spatial distribution of structurally diverse plant hormones: A comprehensive review
Journal of Experimental Botany,
Год журнала:
2024,
Номер
75(22), С. 6980 - 6997
Опубликована: Сен. 12, 2024
Abstract
Plant
hormones
are
essential
and
structurally
diverse
molecules
that
regulate
various
aspects
of
plant
growth,
development,
stress
responses.
However,
the
precise
analysis
in
complex
biological
samples
poses
a
challenge
due
to
their
low
concentrations,
dynamic
levels,
intricate
spatial
distribution.
Moreover,
complexity
interconnectedness
hormone
signaling
networks
make
it
difficult
simultaneously
trace
multiple
distributions.
In
this
review,
we
provide
an
overview
currently
recognized
small-molecule
hormones,
signal
peptide
growth
regulators,
along
with
analytical
methods
employed
for
analysis.
We
delve
into
latest
advancements
mass
spectrometry
imaging
situ
fluorescence
techniques,
which
enable
examination
distribution
hormones.
The
advantages
disadvantages
these
techniques
further
discussed.
Finally,
propose
potential
avenues
enhance
our
understanding
biology.
Язык: Английский
Advancing our understanding of root development: Technologies and insights from diverse studies
PLANT PHYSIOLOGY,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 17, 2024
Understanding
root
development
is
critical
for
enhancing
plant
growth
and
health,
advanced
technologies
are
essential
unraveling
the
complexities
of
these
processes.
In
this
review,
we
highlight
select
technological
innovations
in
study
development,
with
a
focus
on
transformative
impact
single-cell
gene
expression
analysis.
We
provide
high-level
overview
recent
advancements,
illustrating
how
RNA
sequencing
(scRNA-seq)
has
become
pivotal
tool
biology.
scRNA-seq
revolutionized
biology
by
enabling
detailed,
cell-specific
analysis
expression.
This
allowed
researchers
to
create
comprehensive
atlases,
predict
cell
map
regulatory
networks
(GRNs)
unprecedented
precision.
Complementary
technologies,
such
as
multimodal
profiling
bioinformatics,
further
enrich
our
understanding
cellular
dynamics
interactions.
Innovations
imaging
modeling,
combined
genetic
tools
like
CRISPR,
continue
deepen
knowledge
formation
function.
Moreover,
integration
biosensors
microfluidic
devices
ability
plant-microbe
interactions
phytohormone
signaling
at
high
resolution.
These
collectively
more
system
architecture
its
regulation
environmental
factors.
As
evolve,
they
promise
drive
breakthroughs
science,
substantial
implications
agriculture
sustainability.
Язык: Английский
MALDI Mass Spectrometry Imaging of Metabolites in Cereal Grains and Legumes: Repurposing for Industrial Applications
Food Reviews International,
Год журнала:
2024,
Номер
unknown, С. 1 - 22
Опубликована: Дек. 20, 2024
Cereal
grains
and
legumes
are
major
sources
of
health-promoting
nutrients
in
our
daily
diet
key
ingredients
food
production.
However,
they
also
subjected
to
contamination
by
foreign
substances
such
as
mycotoxins,
pesticides,
acrylamide.
Analytical
techniques
methods
for
detecting
quantifying
metabolites
plants
mass
spectrometry
well-established,
but
disregard
spatial
information.
In
contrast,
matrix-assisted
laser
desorption/ionisation
imaging
(MALDI-MSI)
is
a
powerful
technique
study
the
distribution
molecules
within
plant
tissues,
providing
valuable
insights
into
biology
physiology.
To
date,
translation
this
technology
industrial
applications
seldom
explored.
This
review
article
introduces
how
MALDI-MSI
can
revolutionise
traditional
approaches
cereal
legumes.
The
principles
typical
workflow,
particularly
sample
preparation
were
discussed.
explores
previous
implementations
ag-food
research,
opportunities
it
presents
seed
crop
applications.
open
gates
technological
advances
improve
analysis,
processing
regulatory
systems
grain
legume
industry.
Язык: Английский