MALDI Mass Spectrometry Imaging of Metabolites in Cereal Grains and Legumes: Repurposing for Industrial Applications DOI Creative Commons
David C.W. Lau, Leigh Donnellan, Matthew T. Briggs

et al.

Food Reviews International, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 22

Published: Dec. 20, 2024

Cereal grains and legumes are major sources of health-promoting nutrients in our daily diet key ingredients food production. However, they also subjected to contamination by foreign substances such as mycotoxins, pesticides, acrylamide. Analytical techniques methods for detecting quantifying metabolites plants mass spectrometry well-established, but disregard spatial information. In contrast, matrix-assisted laser desorption/ionisation imaging (MALDI-MSI) is a powerful technique study the distribution molecules within plant tissues, providing valuable insights into biology physiology. To date, translation this technology industrial applications seldom explored. This review article introduces how MALDI-MSI can revolutionise traditional approaches cereal legumes. The principles typical workflow, particularly sample preparation were discussed. explores previous implementations ag-food research, opportunities it presents seed crop applications. open gates technological advances improve analysis, processing regulatory systems grain legume industry.

Language: Английский

Mini Review: Highlight of Recent Advances and Applications of MALDI Mass Spectrometry Imaging in 2024 DOI Open Access
Yuen T. Ngai, David C.W. Lau, Parul Mittal

et al.

Analytical Science Advances, Journal Year: 2025, Volume and Issue: 6(1)

Published: May 10, 2025

Abstract Matrix‐assisted laser desorption/ionisation mass spectrometry imaging (MALDI‐MSI) is an emerging tool that allows visualisation of hundreds analytes unbiasedly in a single experiment. This paper highlights the adaptations MALDI‐MSI different context 2024, such as clinical diagnostic, pharmacology, forensics applications, plant metabolism and biology. Challenges advancements were also discussed regarding sample preparation, instrumentations, data analysis, integration machine learning trend cell resolution multi‐omics. There are still rooms for improvements sensitivity, spatial resolution, acquisition algorithm across multi‐omics to enable at subcellular level.

Language: Английский

Citations

0
Imaging the spatial distribution of structurally diverse plant hormones: A comprehensive review DOI
Lulu Chen, Yue Zhang,

Yufen Bu

et al.

Journal of Experimental Botany, Journal Year: 2024, Volume and Issue: 75(22), P. 6980 - 6997

Published: Sept. 12, 2024

Abstract Plant hormones are essential and structurally diverse molecules that regulate various aspects of plant growth, development, stress responses. However, the precise analysis in complex biological samples poses a challenge due to their low concentrations, dynamic levels, intricate spatial distribution. Moreover, complexity interconnectedness hormone signaling networks make it difficult simultaneously trace multiple distributions. In this review, we provide an overview currently recognized small-molecule hormones, signal peptide growth regulators, along with analytical methods employed for analysis. We delve into latest advancements mass spectrometry imaging situ fluorescence techniques, which enable examination distribution hormones. The advantages disadvantages these techniques further discussed. Finally, propose potential avenues enhance our understanding biology.

Language: Английский

Citations

2
Advancing our understanding of root development: Technologies and insights from diverse studies DOI
Núria S. Coll, Miguel Á. Moreno-Risueno, Lucia C. Strader

et al.

PLANT PHYSIOLOGY, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 17, 2024

Understanding root development is critical for enhancing plant growth and health, advanced technologies are essential unraveling the complexities of these processes. In this review, we highlight select technological innovations in study development, with a focus on transformative impact single-cell gene expression analysis. We provide high-level overview recent advancements, illustrating how RNA sequencing (scRNA-seq) has become pivotal tool biology. scRNA-seq revolutionized biology by enabling detailed, cell-specific analysis expression. This allowed researchers to create comprehensive atlases, predict cell map regulatory networks (GRNs) unprecedented precision. Complementary technologies, such as multimodal profiling bioinformatics, further enrich our understanding cellular dynamics interactions. Innovations imaging modeling, combined genetic tools like CRISPR, continue deepen knowledge formation function. Moreover, integration biosensors microfluidic devices ability plant-microbe interactions phytohormone signaling at high resolution. These collectively more system architecture its regulation environmental factors. As evolve, they promise drive breakthroughs science, substantial implications agriculture sustainability.

Language: Английский

Citations

1
MALDI Mass Spectrometry Imaging of Metabolites in Cereal Grains and Legumes: Repurposing for Industrial Applications DOI Creative Commons
David C.W. Lau, Leigh Donnellan, Matthew T. Briggs

et al.

Food Reviews International, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 22

Published: Dec. 20, 2024

Cereal grains and legumes are major sources of health-promoting nutrients in our daily diet key ingredients food production. However, they also subjected to contamination by foreign substances such as mycotoxins, pesticides, acrylamide. Analytical techniques methods for detecting quantifying metabolites plants mass spectrometry well-established, but disregard spatial information. In contrast, matrix-assisted laser desorption/ionisation imaging (MALDI-MSI) is a powerful technique study the distribution molecules within plant tissues, providing valuable insights into biology physiology. To date, translation this technology industrial applications seldom explored. This review article introduces how MALDI-MSI can revolutionise traditional approaches cereal legumes. The principles typical workflow, particularly sample preparation were discussed. explores previous implementations ag-food research, opportunities it presents seed crop applications. open gates technological advances improve analysis, processing regulatory systems grain legume industry.

Language: Английский

Citations

1