Gut microbiota and intervertebral disc degeneration: A Bidirectional Two-Sample Mendelian Randomization Study DOI Creative Commons

Ziming Geng,

Jian Wang, Guangdong Chen

и другие.

Research Square (Research Square), Год журнала: 2023, Номер unknown

Опубликована: Июнь 27, 2023

Abstract BACKGROUND Although previous studies have suggested a close association between gut microbiota(GM) and intervertebral disc degeneration(IVDD), the causal relationship them remains unclear.Hence,we thoroughly investigate their by means of two-sample mendelian randomization (MR) study, aiming to determine impact microbiota on risk developing degeneration. METHODS Summary data from genome-wide (GWAS) GM (the MiBioGen) IVDD FinnGen biobank) been acquired.The inverse variance weighted (IVW) method was utilized as primary MR analysis approach. Weighted median (WME), MR-Egger regression, mode, simple mode were used supplements. The pleiotropy residual sum outlier (MR-PRESSO) regression performed assess horizontal pleiotropy. Cochran's Q test evaluated heterogeneity.Leave-one-out sensitivity further conducted reliability relationship. A reverse potential causation. RESULTS We identified nine microbial taxa that causally associated with IVDD( P < 0.05).Following Benjamini-Hochberg corrected test, phylum Bacteroidetes higher remained significant(IVW FDR-corrected = 0.0365). results Cochrane did not indicate heterogeneity ( > 0.05). Additionally, both intercept MR-PRESSO global revealed our influenced Furthermore, leave-one-out substantiated relationship.In analysis, no evidence found suggest has an microbiota. CONCLUSION Our validate particular IVDD, thus providing fresh insights into microbiota-mediated mechanism laying groundwork for research targeted preventive measures.

Язык: Английский

Genetic analysis of the causal relationship between gut microbiota and intervertebral disc degeneration: a two-sample Mendelian randomized study DOI Creative Commons
Daqiang Zheng, Zhiming Wu, Lu Li

и другие.

European Spine Journal, Год журнала: 2023, Номер 33(5), С. 1986 - 1998

Опубликована: Дек. 13, 2023

Abstract Purpose Several recent studies have reported a possible association between gut microbiota and intervertebral disc degeneration; however, no shown causal relationship degeneration. This study was dedicated to investigate the degeneration presence of potentially bacterial traits using two-sample Mendelian randomization. Methods A randomization performed summary statistics from largest available genome-wide meta-analysis conducted by MiBioGen consortium. Summary were obtained FinnGen consortium R8 release data. Five basic methods MR-PRESSO used examine associations. The results Cochran's Q quantify heterogeneity instrumental variables. Results By analysis, 10 associated with identified: genus Eubacterium coprostanoligenes group, Lachnoclostridium, unknown id.2755, Marvinbryantia, Ruminococcaceae UCG003, family Rhodospirillaceae, id.959, order Rhodospirillales, Lachnospiraceae NK4A136 grou, brachy group. Conclusion Randomization found effect degeneration, we summarize mechanisms action in context existing studies. However, additional research is essential fully understand contribution genetic factors dynamics its impact on

Язык: Английский

Процитировано

7
The Role of the Gut Microbiome in Orthopedic Surgery—a Narrative Review DOI
David Hiltzik, Alyssa M. Goodwin, Steven S. Kurapaty

и другие.

Current Reviews in Musculoskeletal Medicine, Год журнала: 2023, Номер 17(2), С. 37 - 46

Опубликована: Дек. 22, 2023

Язык: Английский

Процитировано

7
Unveiling the Gut-Disc Axis: How Microbiome Dysbiosis Accelerates Intervertebral Disc Degeneration DOI Creative Commons

Yu Sun,

Zhaoyong Li, Jiahao Duan

и другие.

Journal of Inflammation Research, Год журнала: 2024, Номер Volume 17, С. 8271 - 8280

Опубликована: Ноя. 1, 2024

Abstract: The gut microbiome (GM), often referred to as the second genome of human body, plays a crucial role in various metabolic processes and mediates development numerous diseases. Intervertebral disc degeneration (IDD) is an age-related degenerative spinal disease characterized by loss height, hydration, integrity, leading pain reduced mobility. Although pathogenesis IDD not fully understood, recent studies suggest that dysbiosis may accelerate progression through multiple mechanisms. This article begins discussing potential relationship between GM diseases, followed comprehensive review regulatory mechanisms skeletal diseases within gut-disc axis framework. Furthermore, it explores three pathways which mediate IDD: immunomodulation, bacterial translocation colonization, decomposition absorption intestinal metabolites. These can disrupt cell homeostasis promote changes. Finally, this paper summarizes for first time therapeutic approaches delaying targeting axis, providing new insights into regenerative repair strategies IDD. Keywords: intervertebral degeneration, microbiome, inflammation, immunity, metabolism

Язык: Английский

Процитировано

2
Analysis of Intestinal Bacterial Microbiota in Individuals with and without Chronic Low Back Pain DOI Creative Commons
Antonio Martins Tieppo,

Júlia Silva Tieppo,

Luiz Antônio Rivetti

и другие.

Current Issues in Molecular Biology, Год журнала: 2024, Номер 46(7), С. 7339 - 7352

Опубликована: Июль 12, 2024

Low back pain is a health problem that represents the greatest cause of years lived with disability. This research seeks to evaluate bacterial composition intestinal microbiota two similar groups: one chronic low (PG) and control group (CG). Clinical data from 73 participants genome sequencing stool samples were analyzed. There 40 individuals in PG 33 CG, aged between 20 50 body mass index up 30 kg/m2. Thus, intragroup alpha diversity intergroup beta The significant results (p < 0.05) showed greater species richness compared CG. Additionally, abundance Clostridium difficile was found along 52 significantly different average relative abundances groups (adjusted p 0.05), 36 more abundant 16 We are first unveil differences who non-elderly, non-obese without any other serious diseases. It could be reference for possible signature pain.

Язык: Английский

Процитировано

0
A new target for treating intervertebral disk degeneration: gut microbes DOI Creative Commons
Kaizhong Wang, Xiangyan Liu,

Huagui Huang

и другие.

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Ноя. 29, 2024

Intervertebral disk degeneration (IDD) is a common clinical spinal disease and one of the main causes low back pain (LBP). Generally speaking, IDD considered natural degenerative process with age. However, deepening research, people have discovered that not only related to age, but also has many factors can induce accelerate its progression. In addition, pathogenesis remains unclear, resulting in limited traditional treatment methods cannot effectively prevent treat IDD. Conservative may lead patients’ dependence on drugs, relief effect obvious. Similarly, surgical highly invasive, longer recovery time higher recurrence rate. With exploration, intestinal microorganisms are an important symbiotic microbial community human body closely occurrence development various diseases. Changes their metabolites affect body’s inflammatory response, immune regulation, metabolic processes, thereby affecting health intervertebral disk. this context, gut microbiota received considerable attention as potential target for delaying or treating This article first introduces impact microbes distal organs, then focuses three mechanisms by which influence Finally, we summarized interfering metabolites. Further understanding between will help formulate reasonable strategies achieve ideal therapeutic effects.

Язык: Английский

Процитировано

0
Bone morphogenetic proteins, DNA methylation, and gut microbiota interaction in lumbar disc degeneration: A multi‐omics Mendelian randomization study DOI Creative Commons
Xiangyu Li, Pengyun Wang,

Qijun Wang

и другие.

JOR Spine, Год журнала: 2024, Номер 7(4)

Опубликована: Дек. 1, 2024

Abstract Background Lumbar disc degeneration (LDD) is a ubiquitous finding in low back pain. Many different etiology factors may explain the LDD process, such as bone morphogenetic proteins (BMPs), DNA methylation, and gut microbiota. Until recently mechanisms underlying process have been elusive. Methods BMP‐related genes were extracted from GeneCards database. The transcriptome dataset was obtained Gene Expression Omnibus. We used linear regression meta‐analysis to screen integrate differentially expressed associated with BMPs LDD. Genome‐wide association studies (GWASs) of FinnGen UKBB. expression quantitative trait loci (eQTLs) methylation blood identified via summary data‐based Mendelian randomization (SMR) method, possible BMP their regulatory elements risk prioritized. Intestinal eQTLs fecal microbial QTLs (mbQTLs) integrated, potential interactions between gene host intestinal tissue microbiota revealed through SMR colocalization analysis. GWAS catalog (GCST90246169) validate results. Results A five datasets that 113 control tissues. Seven selected candidate pathogenic three‐step method: CREB1 , BMP6 PTCH1 GLI1 MEG3 GALNS NF1 . analysis also genes: HFE MET MAPK3 NPC1 GDF5 correlation tissues verified by eQTL‐mbQTL colocalization. Conclusion This multi‐omics study are regulated methylation. There genetic differences These findings provide evidence for new therapeutic targets future.

Язык: Английский

Процитировано

0
Gut microbiota and intervertebral disc degeneration: A Bidirectional Two-Sample Mendelian Randomization Study DOI Creative Commons

Ziming Geng,

Jian Wang, Guangdong Chen

и другие.

Research Square (Research Square), Год журнала: 2023, Номер unknown

Опубликована: Июнь 27, 2023

Abstract BACKGROUND Although previous studies have suggested a close association between gut microbiota(GM) and intervertebral disc degeneration(IVDD), the causal relationship them remains unclear.Hence,we thoroughly investigate their by means of two-sample mendelian randomization (MR) study, aiming to determine impact microbiota on risk developing degeneration. METHODS Summary data from genome-wide (GWAS) GM (the MiBioGen) IVDD FinnGen biobank) been acquired.The inverse variance weighted (IVW) method was utilized as primary MR analysis approach. Weighted median (WME), MR-Egger regression, mode, simple mode were used supplements. The pleiotropy residual sum outlier (MR-PRESSO) regression performed assess horizontal pleiotropy. Cochran's Q test evaluated heterogeneity.Leave-one-out sensitivity further conducted reliability relationship. A reverse potential causation. RESULTS We identified nine microbial taxa that causally associated with IVDD( P < 0.05).Following Benjamini-Hochberg corrected test, phylum Bacteroidetes higher remained significant(IVW FDR-corrected = 0.0365). results Cochrane did not indicate heterogeneity ( > 0.05). Additionally, both intercept MR-PRESSO global revealed our influenced Furthermore, leave-one-out substantiated relationship.In analysis, no evidence found suggest has an microbiota. CONCLUSION Our validate particular IVDD, thus providing fresh insights into microbiota-mediated mechanism laying groundwork for research targeted preventive measures.

Язык: Английский

Процитировано

0