Theoretical and Applied Genetics, Год журнала: 2024, Номер 137(1)
Опубликована: Янв. 1, 2024
Язык: Английский
Science China Life Sciences, Год журнала: 2022, Номер 65(9), С. 1718 - 1775
Опубликована: Авг. 24, 2022
Язык: Английский
Процитировано
127
Molecular Plant, Год журнала: 2024, Номер 17(3), С. 438 - 459
Опубликована: Фев. 2, 2024
Язык: Английский
Процитировано
20
Plant Biotechnology Journal, Год журнала: 2021, Номер 20(1), С. 75 - 88
Опубликована: Сен. 6, 2021
Summary The AGAMOUS‐LIKE6 ( AGL6 )‐like genes are ancient MADS‐box and functionally studied in a few model plants. knowledge of these wheat remains limited. Here, by studying ‘double homoeolog mutant’ the gene tetraploid wheat, we showed that was required for development all four whorls floral organs with dosage‐dependent effect on floret fertility. Yeast two‐hybrid analyses detected interactions classes proteins ABCDE organ development. found to interact several additional proteins, including G protein β γ (DEP1) subunits. Analysis DEP1‐B mutant significant reduction spikelet number per spike while overexpression common increased hence grain spike. RNA‐seq analysis identified regulation meristem activity AGL6, such as FUL2 TaMADS55 . Our work therefore extensively updated proposed an alternative approach improve yield manipulating gene.
Язык: Английский
Процитировано
58
Nature Communications, Год журнала: 2023, Номер 14(1)
Опубликована: Март 10, 2023
Abstract Torreya plants produce dry fruits with assorted functions. Here, we report the 19-Gb chromosome-level genome assembly of T. grandis . The is shaped by ancient whole-genome duplications and recurrent LTR retrotransposon bursts. Comparative genomic analyses reveal key genes involved in reproductive organ development, cell wall biosynthesis seed storage. Two encoding a C 18 Δ 9 -elongase 20 5 -desaturase are identified to be responsible for sciadonic acid both present diverse plant lineages except angiosperms. We demonstrate that histidine-rich boxes crucial its catalytic activity. Methylome analysis reveals methylation valleys harbor associated important activities, including lipid biosynthesis. Moreover, development accompanied DNA changes possibly fuel energy production. This study provides resources elucidates evolutionary mechanism land plants.
Язык: Английский
Процитировано
43
Plant Biotechnology Journal, Год журнала: 2023, Номер 21(10), С. 1990 - 2001
Опубликована: Авг. 17, 2023
Summary Plant breeding is constrained by trade‐offs among different agronomic traits the pleiotropic nature of many genes. Genes that contribute to two or more favourable with no penalty on yield are rarely reported, especially in wheat. Here, we describe editing a wheat auxin response factor TaARF12 using CRISPR/Cas9 rendered shorter plant height larger spikes. Changes architecture enhanced grain number per spike up 14.7% significantly higher thousand‐grain weight and 11.1% increase under field trials. Weighted Gene Co‐Expression Network Analysis (WGCNA) spatial–temporal transcriptome profiles revealed hub genes: RhtL1 , DELLA domain‐free Rht‐1 paralog, which was up‐regulated peduncle, TaNGR5 an organ size regulator rachis, taarf12 plants. The up‐regulation peduncle suggested repression GA signalling, whereas may promote response, working model supported differential expression patterns biogenesis genes tissues. Thus, complemented reduction spikes gave yield. Manipulation represent new strategy trait pyramiding for improvement
Язык: Английский
Процитировано
26
Genome biology, Год журнала: 2023, Номер 24(1)
Опубликована: Май 12, 2023
Abstract Background Plant architecture associated with increased grain yield and adaptation to the local environments is selected during wheat ( Triticum aestivum ) breeding. The internode length of individual stems tiller plants are important for determination plant architecture. However, few studies have explored genetic basis these traits. Results Here, we conduct a genome-wide association study (GWAS) dissect geographical differentiation traits in 306 worldwide accessions including both landraces traditional varieties. We determine changes haplotypes genomic regions frequency 831 that either introduced from other countries or developed China last two decades. identify 83 loci one trait, while remaining 247 pleiotropic. also find 163 under strong selective sweep. GWAS results demonstrate independent regulation consistent plants. This makes it possible obtain ideal haplotype combinations four internodes. distribution explains observed differences among accessions. Conclusion provides insights into It will facilitate gene functional analysis molecular design
Язык: Английский
Процитировано
24
Journal of genetics and genomics/Journal of Genetics and Genomics, Год журнала: 2023, Номер 50(11), С. 835 - 845
Опубликована: Март 11, 2023
Wheat is the most widely grown crop globally, providing 20% of daily consumed calories and protein content around world. With growing global population frequent occurrence extreme weather caused by climate change, ensuring adequate wheat production essential for food security. The architecture inflorescence plays a crucial role in determining grain number size, which key trait improving yield. Recent advances genomics gene cloning techniques have improved our understanding spike development its applications breeding practices. Here, we summarize genetic regulation network governing formation, strategies used identifying studying factors affecting architecture, progress made applications. Additionally, highlight future directions that will aid regulatory mechanistic study determination targeted yield improvement.
Язык: Английский
Процитировано
23
Nature Communications, Год журнала: 2024, Номер 15(1)
Опубликована: Янв. 5, 2024
Abstract Floral organ shape and size in cereal crops can affect grain yield, so genes that regulate their development are promising breeding targets. The lemma, which protects inner floral organs, physically constrain growth; while the awn, a needle-like extension of creates photosynthate to developing grain. Although several modules controlling awn/lemma growth rice have been characterized, these processes, relationships between them, not well understood for barley wheat. Here, we demonstrate E-class gene HvMADS1 positively regulates awn length lemma width, affecting weight. Cytological data indicates promotes by promoting cell proliferation, multi-omics reveals target associated with cycle, phytohormone signaling, developmental processes. We define two potential targets regulation, HvSHI HvDL , whose knockout mutants mimic and/or phenotypes mads1 mutants. Additionally, interacts APETALA2 (A-class) synergistically activate downstream barley. Notably, find MADS1 function remains conserved wheat, proliferation increase length. These findings extend our understanding provide insights Triticeae crop improvement strategies.
Язык: Английский
Процитировано
9
BMC Genomics, Год журнала: 2025, Номер 26(1)
Опубликована: Янв. 20, 2025
Paeonia section Moutan DC. is a significant perennial subshrub, the ornamental value of which heavily depends on type flower it possesses. MADS-box transcription factors have particular impact intricate process floral organ development and differentiation. The release whole-genome data from ostii now allows us to conduct thorough investigation tree peony gene family. In this study, we identified 110 genes in that were classified into 5 subgroups. Gene structure, domain motif analyses revealed conservation structure these Analysis cis-acting elements PoMADS contained different kinds hormones stress-related their promoter regions. Quantitative real-time PCR analysis was employed validate expression patterns some related development. Genome collinearity with Arabidopsis grape during evolution. A total 857 SSRs by analysing genome sequences genes. We additionally created protein‒protein interaction networks for proteins analysed could interact among PoMADSs thaliana grape. These findings offer fundamental insights understanding function family, can aid selection breeding varieties high addition supporting organogenesis.
Язык: Английский
Процитировано
1
New Phytologist, Год журнала: 2022, Номер 235(4), С. 1515 - 1530
Опубликована: Май 11, 2022
Summary Plant architecture is a key determinant of crop productivity and adaptation. The highly conserved microRNA319 (miR319) family functions in various biological processes, but little known about how miR319 regulates plant wheat ( Triticum aestivum ). Here, we determined that the miR319/TaGAMYB3 module controls grain yield common wheat. Repressing tae‐miR319 using short tandem target mimics resulted favorable traits, including increased height, reduced tiller number, enlarged spikes flag leaves, thicker culms, as well enhanced field plot tests. Overexpressing had opposite effects on yield. Although both TaPCF8 TaGAMYB3 were identified genes, genetic complementation assays demonstrated only miR319‐resistant rTaGAMYB3 ) abolished tae‐miR319‐mediated growth inhibition leaves spikes. transcriptional activator downstream TaPSKR1 , TaXTH23 TaMADS5 TaMADS51 by binding to their promoters. Furthermore, physically interacts with TaBA1, an important regulator spike development, additively activate transcription genes such . Our findings provide insight into improves
Язык: Английский
Процитировано
25