Frontiers in Plant Science,
Год журнала:
2023,
Номер
14
Опубликована: Сен. 1, 2023
The
deployment
of
disease
resistance
genes
is
currently
the
most
economical
and
environmentally
sustainable
method
crop
protection.
However,
can
rapidly
break
down
because
constant
pathogen
evolution,
particularly
when
they
are
deployed
singularly.
Polygenic
is,
therefore,
considered
durable,
but
combining
maintaining
these
by
breeding
a
laborious
process
as
effective
usually
unlinked.
polygenic
with
single-locus
inheritance
promising
innovation
that
overcomes
difficulties
while
enhancing
durability.
Because
major
advances
in
genomic
technologies,
increasing
numbers
plant
have
been
cloned,
enabling
development
transgene
stacks
(RTGSs)
encode
multiple
all
located
at
single
genetic
locus.
Gene
encoding
five
stem
rust
now
developed
transgenic
wheat
offer
both
simplicity
potential
similar
resources
phytopathogens
has
advanced
effector
gene
isolation
and,
some
instances,
enabled
functional
validation
individual
RTGS.
Here,
pathosystem
used
an
illustrative
example
how
host
instrumental
RTGS,
which
strategy
applicable
to
many
other
agricultural
species.
Nature,
Год журнала:
2024,
Номер
633(8031), С. 848 - 855
Опубликована: Авг. 14, 2024
Bread
wheat
(Triticum
aestivum)
is
a
globally
dominant
crop
and
major
source
of
calories
proteins
for
the
human
diet.
Compared
with
its
wild
ancestors,
modern
bread
shows
lower
genetic
diversity,
caused
by
polyploidisation,
domestication
breeding
bottlenecks
Plant Biotechnology Journal,
Год журнала:
2022,
Номер
20(9), С. 1730 - 1742
Опубликована: Май 14, 2022
A
resistance
gene
atlas
is
an
integral
component
of
the
breeder's
arsenal
in
fight
against
evolving
pathogens.
Thanks
to
high-throughput
sequencing,
catalogues
genes
can
be
assembled
even
crop
species
with
large
and
polyploid
genomes.
Here,
we
report
on
capture
sequencing
assembly
homologs
a
diversity
panel
907
winter
wheat
genotypes
comprising
ex
situ
genebank
accessions
current
elite
cultivars.
In
addition,
use
accurate
long-read
chromosome
conformation
construct
chromosome-scale
genome
sequence
cv.
Attraktion,
variety
representative
European
wheat.
We
illustrate
value
our
resource
for
breeders
geneticists
by
(i)
comparing
complements
plant
genetic
resources
varieties
(ii)
conducting
genome-wide
associations
scans
(GWAS)
fungal
diseases
yellow
rust
leaf
using
reference-based
reference-free
GWAS
approaches.
The
content
under
peaks
was
scrutinized
Attraktion.
Improved
agricultural
and
industrial
production
organisms
are
required
to
meet
the
future
global
food
demands
minimize
effects
of
climate
change.
A
new
resource
for
crop
microbe
improvement,
designated
FIND-IT
(Fast
Identification
Nucleotide
variants
by
droplet
DigITal
PCR),
provides
ultrafast
identification
isolation
predetermined,
targeted
genetic
in
a
screening
cycle
less
than
10
days.
Using
large-scale
sample
pooling
combination
with
digital
PCR
(ddPCR)
greatly
increases
size
low–mutation
density
screenable
variant
libraries
probability
identifying
interest.
The
method
is
validated
totaling
500,000
barley
(
Hordeum
vulgare
)
individuals
isolating
more
125
gene
knockout
lines
miRNA
or
promoter
enabling
functional
analysis.
directly
applicable
elite
breeding
pipelines
time-consuming
technical
steps
accelerate
evolution
germplasm.
Plant Biotechnology Journal,
Год журнала:
2023,
Номер
22(1), С. 66 - 81
Опубликована: Авг. 24, 2023
Summary
Rye
(
Secale
cereale
),
a
valuable
relative
of
wheat,
contains
abundant
powdery
mildew
resistance
Pm
)
genes.
Using
physical
mapping,
transcriptome
sequencing,
barley
stripe
mosaic
virus‐induced
gene
silencing,
ethyl
methane
sulfonate
mutagenesis,
and
stable
transformation,
we
isolated
validated
two
coiled‐coil,
nucleotide‐binding
site
leucine‐rich
repeat
(CC‐NBS‐LRR)
alleles,
PmTR1
PmTR3
,
located
on
rye
chromosome
6RS
from
different
triticale
lines.
confers
age‐related
starting
the
three‐leaf
stage,
whereas
its
allele,
typical
all‐stage
resistance,
which
may
be
associated
with
their
differential
expression
patterns.
Overexpression
in
Nicotiana
benthamiana
showed
that
CC,
CC‐NBS,
CC‐LRR
fragments
PMTR1
induce
cell
death,
PMTR3
CC
full‐length
perform
this
function.
Luciferase
complementation
imaging
pull‐down
assays
revealed
distinct
interaction
activities
between
NBS
fragments.
Our
study
elucidates
novel
rye‐derived
genes
derivative
germplasm
resources
provides
insights
into
mechanism
can
aid
improvement
against
wheat
mildew.
Plants,
Год журнала:
2025,
Номер
14(7), С. 1007 - 1007
Опубликована: Март 23, 2025
Climate
change
is
driving
the
spread
of
transboundary
wheat
diseases,
necessitating
development
resilient
varieties
for
sustainable
agriculture.
Wheat
rusts,
including
leaf
rust
(LR),
yellow
(YR),
and
stem
(SR),
remain
among
most
economically
significant
causing
substantial
yield
losses
worldwide.
Enhancing
genetic
diversity
by
identifying
deploying
resistance
genes
crucial
durable
in
breeding
programs.
This
study
aimed
to
identify
quantitative
trait
loci
(QTL)
associated
with
CIMMYT
line
Kasuku,
released
Kenya
2018.
A
recombinant
inbred
(RIL)
population
(181
lines)
derived
from
Kasuku
(triple
rust-resistant)
Apav#1
rust-susceptible)
was
evaluated
under
artificial
LR
YR
epidemics
Mexico
SR
Kenya.
QTL
mapping
using
genotyping-by-sequencing
(DArTSeq)
phenotypic
data
identified
four
major
loci:
QLrYrSr.cim-1BL
(Lr46/Yr29/Sr58)
on
1BL,
conferring
LR,
YR,
SR;
QLrYr.cim-2AS
(Yr17/Lr37)
2AS,
providing
resistance;
QLrYr.cim-3AL
3AL;
QLrYrSr.cim-6AL
6AL,
representing
novel
multiple
resistances.
Additionally,
minor
were
also
identified:
(QLr.cim-2DS
2DS,
QLr.cim-6DS
6DS),
(QYrKen.cim-3DS
3DS,
QYrKen.cim-6BS
6BS),
(QSr.cim-2BS
2BS,
QSr.cim-5AL
5AL,
QSr.cim-6AS
6AS).
RILs
carrying
these
combinations
exhibited
reductions
severity.
Flanking
markers
are
being
used
develop
Kompetitive
Allele-Specific
PCR
(KASP)
fine
marker-assisted
selection
(MAS).
These
findings
contribute
strategic
deployment
programs,
facilitating
pathogens.
