CRISPR/Cas genome editing for cotton precision breeding: mechanisms, advances, and prospects
Journal of Cotton Research,
Год журнала:
2025,
Номер
8(1)
Опубликована: Фев. 3, 2025
Abstract
Cotton
(
Gossypium
hirsutum
L.)
is
one
of
the
most
important
global
crops
that
supports
textile
industry
and
provides
a
living
for
millions
farmers.
The
constantly
increasing
demand
needs
significant
rise
in
cotton
production.
Genome
editing
technology,
specifically
with
clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)/CRISPR-associated
protein
(Cas)
tools,
has
opened
new
possibilities
trait
development
cotton.
It
allows
precise
efficient
manipulation
within
genome
when
compared
other
genetic
engineering
tools.
Current
developments
CRISPR/Cas
including
prime
editing,
base
multiplexing
have
expanded
scope
traits
breeding
can
be
targeted.
been
employed
to
generate
effectively
CRISPRized
plants
enhanced
agronomic
traits,
fiber
yield
quality,
oil
improvement,
stress
resistance,
nutrition.
Here
we
summarized
various
target
genes
which
successfully
altered
However,
some
challenges
remain,
tetraploid
having
redundant
gene
sets
homologs
making
editing.
To
ensure
specificity
avoiding
off-target
effects,
need
optimize
parameters
such
as
site,
guide
RNA
design,
choosing
right
Cas
variants.
We
outline
future
prospects
breeding,
suggesting
areas
further
research
innovation.
A
combination
speed
might
useful
fastening
potentials
create
customized
cultivars
meet
higher
demands
agriculture
industry.
Язык: Английский
Metal transport proteins and transcription factor networks in plant responses to cadmium stress
Plant Cell Reports,
Год журнала:
2024,
Номер
43(9)
Опубликована: Авг. 17, 2024
Язык: Английский
Genome-Wide Identification of the WRKY Gene Family in Four Cotton Varieties and the Positive Role of GhWRKY31 in Response to Salt and Drought Stress
Plants,
Год журнала:
2024,
Номер
13(13), С. 1814 - 1814
Опубликована: Июль 1, 2024
The
WRKY
gene
family
is
ubiquitously
distributed
in
plants,
serving
crucial
functions
stress
responses.
Nevertheless,
the
structural
organization
and
evolutionary
dynamics
of
genes
cotton
have
not
been
fully
elucidated.
In
this
study,
a
total
112,
119,
217,
222
were
identified
Gossypium
arboreum,
raimondii,
hirsutum,
barbadense,
respectively.
These
670
categorized
into
seven
distinct
subgroups
unequally
across
chromosomes.
Examination
conserved
motifs,
domains,
cis-acting
elements,
architecture
collectively
highlighted
conservation
divergence
within
cotton.
Analysis
synteny
collinearity
further
confirmed
instances
expansion,
duplication,
loss
events
among
during
evolution.
Furthermore,
GhWRKY31
transgenic
Arabidopsis
exhibited
heightened
germination
rates
longer
root
lengths
under
drought
salt
stress.
Silencing
led
to
reduced
levels
ABA,
proline,
POD,
SOD,
along
with
downregulated
expression
stress-responsive
genes.
Yeast
one-hybrid
molecular
docking
assays
binding
capacity
W
box
GhABF1,
GhDREB2,
GhRD29.
findings
offer
systematic
comprehensive
insight
patterns
WRKYs,
proposing
suitable
regulatory
framework
for
developing
cultivars
enhanced
resilience
salinity
Язык: Английский
The MYB-bHLH-NRAMP module modulates the cadmium sensitivity of quinoa by regulating cadmium transport and absorption
Journal of Hazardous Materials,
Год журнала:
2025,
Номер
486, С. 137132 - 137132
Опубликована: Янв. 5, 2025
Язык: Английский
Uncovering miRNA-mRNA regulatory modules of cotton in response to cadmium stress
Plant Physiology and Biochemistry,
Год журнала:
2025,
Номер
222, С. 109614 - 109614
Опубликована: Фев. 21, 2025
Язык: Английский
CsbHLH122/CsMYBS3‐CsSUT2 contributes to the rapid accumulation of sugar in the ripening stage of sweet orange (Citrus sinensis)
The Plant Journal,
Год журнала:
2025,
Номер
122(1)
Опубликована: Апрель 1, 2025
SUMMARY
The
sugar
content
serves
as
the
fundamental
metabolic
component
that
determines
both
flavor
quality
and
nutritional
value
of
fruits.
Nevertheless,
regulatory
mechanism
underlying
rapid
accumulation
sugars
during
citrus
fruit
maturation
remains
elusive.
In
this
study,
we
demonstrated
expression
level
sucrose
transporter
CsSUT2
is
increased
ripening
accumulation.
Functional
assays
confirmed
localized
in
plasma
membrane
exhibits
activity.
Homologous
heterologous
overexpression
juice
sacs,
calli,
tomato
resulted
an
increase
content.
Conversely,
virus‐induced
gene
silencing
RNAi‐mediated
led
to
a
decrease
levels
transgenic
tissues.
We
further
identified
CsMYBS3
upstream
transcription
factor
positively
regulates
.
Transgenic
evidence
supported
induction
by
depends
on
transcript
Additionally,
found
CsbHLH122
physically
interacts
with
form
complex,
enhancing
promoter
transcriptional
activity
This
study
expands
our
understanding
function
citrus,
providing
valuable
insights
for
regulating
control
fruit.
Язык: Английский
AINTEGUMENTA-LIKE 5 promotes root and leaf growth by regulating CYCLIN D6;1 in Rehmannia glutinosa
International Journal of Biological Macromolecules,
Год журнала:
2025,
Номер
unknown, С. 144857 - 144857
Опубликована: Июнь 1, 2025
Язык: Английский
Cadmium (Cd) Tolerance and Phytoremediation Potential in Fiber Crops: Research Updates and Future Breeding Efforts
Agronomy,
Год журнала:
2024,
Номер
14(11), С. 2713 - 2713
Опубликована: Ноя. 17, 2024
Heavy
metal
pollution
is
one
of
the
most
devastating
abiotic
factors,
significantly
damaging
crops
and
human
health.
One
serious
problems
it
causes
a
rise
in
cadmium
(Cd)
toxicity.
Cd
highly
toxic
with
negative
biological
role,
enters
plants
via
soil–plant
system.
stress
induces
series
disorders
plants’
morphological,
physiological,
biochemical
processes
initiates
inhibition
seed
germination,
ultimately
resulting
reduced
growth.
Fiber
such
as
kenaf,
jute,
hemp,
cotton,
flax
have
high
industrial
importance
often
face
issue
Various
techniques
been
introduced
to
counter
rising
threats
toxicity,
including
reducing
content
soil,
mitigating
effects
stress,
genetic
improvements
plant
tolerance
against
this
stress.
For
decades,
breeders
trying
develop
Cd-tolerant
fiber
through
identification
transformation
novel
genes.
Still,
complex
mechanism
has
hindered
progress
breeding.
These
are
ideal
candidates
for
phytoremediation
heavy
metals
contaminated
soils.
Hence,
increased
uptake,
accumulation,
translocation
below-ground
parts
(roots)
above-ground
(shoots,
leaves,
stems)
can
help
clean
agricultural
lands
safe
use
food
crops.
Earlier
studies
indicated
that
detoxification,
developing
these
stresses
genes
fruitful
approaches.
This
review
aims
highlight
role
some
conventional
molecular
key
Molecular
mainly
involve
QTL
mapping
GWAS.
However,
more
focus
given
transcriptome
TFs
analysis
explore
potential
genomic
regions
involved
will
serve
source
valuable
information
on
crops,
allowing
further
in-depth
analyses
identify
critical
breeding,
like
engineering
CRISPR/Cas9.
Язык: Английский
Genome-wide analysis of WRKY gene family and the dynamic responses of key WRKY genes involved in cadmium stress in Brassica juncea
Frontiers in Plant Science,
Год журнала:
2024,
Номер
15
Опубликована: Окт. 10, 2024
The
WRKY
transcription
factors
comprise
one
of
the
most
extensive
gene
families
and
serve
as
pivotal
regulators
plant
responses
to
heavy
metal
stress.
They
contribute
significantly
maintaining
growth
development
by
enhancing
tolerance.
However,
research
on
role
genes
in
response
cadmium
(Cd)
stress
mustard
is
minimal.
In
this
study,
we
conducted
a
genome-wide
analysis
family
using
bioinformatics.
results
revealed
that
291
putative
(
Язык: Английский