Metal transport proteins and transcription factor networks in plant responses to cadmium stress

Plant Cell Reports, Journal Year: 2024, Volume and Issue: 43(9)

Published: Aug. 17, 2024

Language: Английский

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CRISPR/Cas genome editing for cotton precision breeding: mechanisms, advances, and prospects

Journal of Cotton Research, Journal Year: 2025, Volume and Issue: 8(1)

Published: Feb. 3, 2025

Abstract Cotton ( Gossypium hirsutum L.) is one of the most important global crops that supports textile industry and provides a living for millions farmers. The constantly increasing demand needs significant rise in cotton production. Genome editing technology, specifically with clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) tools, has opened new possibilities trait development cotton. It allows precise efficient manipulation within genome when compared other genetic engineering tools. Current developments CRISPR/Cas including prime editing, base multiplexing have expanded scope traits breeding can be targeted. been employed to generate effectively CRISPRized plants enhanced agronomic traits, fiber yield quality, oil improvement, stress resistance, nutrition. Here we summarized various target genes which successfully altered However, some challenges remain, tetraploid having redundant gene sets homologs making editing. To ensure specificity avoiding off-target effects, need optimize parameters such as site, guide RNA design, choosing right Cas variants. We outline future prospects breeding, suggesting areas further research innovation. A combination speed might useful fastening potentials create customized cultivars meet higher demands agriculture industry.

Language: Английский

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Genome-Wide Identification of the WRKY Gene Family in Four Cotton Varieties and the Positive Role of GhWRKY31 in Response to Salt and Drought Stress

Plants, Journal Year: 2024, Volume and Issue: 13(13), P. 1814 - 1814

Published: July 1, 2024

The WRKY gene family is ubiquitously distributed in plants, serving crucial functions stress responses. Nevertheless, the structural organization and evolutionary dynamics of genes cotton have not been fully elucidated. In this study, a total 112, 119, 217, 222 were identified Gossypium arboreum, raimondii, hirsutum, barbadense, respectively. These 670 categorized into seven distinct subgroups unequally across chromosomes. Examination conserved motifs, domains, cis-acting elements, architecture collectively highlighted conservation divergence within cotton. Analysis synteny collinearity further confirmed instances expansion, duplication, loss events among during evolution. Furthermore, GhWRKY31 transgenic Arabidopsis exhibited heightened germination rates longer root lengths under drought salt stress. Silencing led to reduced levels ABA, proline, POD, SOD, along with downregulated expression stress-responsive genes. Yeast one-hybrid molecular docking assays binding capacity W box GhABF1, GhDREB2, GhRD29. findings offer systematic comprehensive insight patterns WRKYs, proposing suitable regulatory framework for developing cultivars enhanced resilience salinity

Language: Английский

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The MYB-bHLH-NRAMP module modulates the cadmium sensitivity of quinoa by regulating cadmium transport and absorption

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: 486, P. 137132 - 137132

Published: Jan. 5, 2025

Language: Английский

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Uncovering miRNA-mRNA regulatory modules of cotton in response to cadmium stress

Plant Physiology and Biochemistry, Journal Year: 2025, Volume and Issue: 222, P. 109614 - 109614

Published: Feb. 21, 2025

Language: Английский

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CsbHLH122/CsMYBS3‐CsSUT2 contributes to the rapid accumulation of sugar in the ripening stage of sweet orange (Citrus sinensis)

The Plant Journal, Journal Year: 2025, Volume and Issue: 122(1)

Published: April 1, 2025

SUMMARY The sugar content serves as the fundamental metabolic component that determines both flavor quality and nutritional value of fruits. Nevertheless, regulatory mechanism underlying rapid accumulation sugars during citrus fruit maturation remains elusive. In this study, we demonstrated expression level sucrose transporter CsSUT2 is increased ripening accumulation. Functional assays confirmed localized in plasma membrane exhibits activity. Homologous heterologous overexpression juice sacs, calli, tomato resulted an increase content. Conversely, virus‐induced gene silencing RNAi‐mediated led to a decrease levels transgenic tissues. We further identified CsMYBS3 upstream transcription factor positively regulates . Transgenic evidence supported induction by depends on transcript Additionally, found CsbHLH122 physically interacts with form complex, enhancing promoter transcriptional activity This study expands our understanding function citrus, providing valuable insights for regulating control fruit.

Language: Английский

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AINTEGUMENTA-LIKE 5 promotes root and leaf growth by regulating CYCLIN D6;1 in Rehmannia glutinosa

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 144857 - 144857

Published: June 1, 2025

Language: Английский

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Cadmium (Cd) Tolerance and Phytoremediation Potential in Fiber Crops: Research Updates and Future Breeding Efforts

Agronomy, Journal Year: 2024, Volume and Issue: 14(11), P. 2713 - 2713

Published: Nov. 17, 2024

Heavy metal pollution is one of the most devastating abiotic factors, significantly damaging crops and human health. One serious problems it causes a rise in cadmium (Cd) toxicity. Cd highly toxic with negative biological role, enters plants via soil–plant system. stress induces series disorders plants’ morphological, physiological, biochemical processes initiates inhibition seed germination, ultimately resulting reduced growth. Fiber such as kenaf, jute, hemp, cotton, flax have high industrial importance often face issue Various techniques been introduced to counter rising threats toxicity, including reducing content soil, mitigating effects stress, genetic improvements plant tolerance against this stress. For decades, breeders trying develop Cd-tolerant fiber through identification transformation novel genes. Still, complex mechanism has hindered progress breeding. These are ideal candidates for phytoremediation heavy metals contaminated soils. Hence, increased uptake, accumulation, translocation below-ground parts (roots) above-ground (shoots, leaves, stems) can help clean agricultural lands safe use food crops. Earlier studies indicated that detoxification, developing these stresses genes fruitful approaches. This review aims highlight role some conventional molecular key Molecular mainly involve QTL mapping GWAS. However, more focus given transcriptome TFs analysis explore potential genomic regions involved will serve source valuable information on crops, allowing further in-depth analyses identify critical breeding, like engineering CRISPR/Cas9.

Language: Английский

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Genome-wide analysis of WRKY gene family and the dynamic responses of key WRKY genes involved in cadmium stress in Brassica juncea

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 10, 2024

The WRKY transcription factors comprise one of the most extensive gene families and serve as pivotal regulators plant responses to heavy metal stress. They contribute significantly maintaining growth development by enhancing tolerance. However, research on role genes in response cadmium (Cd) stress mustard is minimal. In this study, we conducted a genome-wide analysis family using bioinformatics. results revealed that 291 putative (

Language: Английский

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