Transcriptome analysis of Stephania yunnanensis and functional validation of CYP80s involved in benzylisoquinoline alkaloids biosynthesis DOI Creative Commons

Wenlong Shi,

Qishuang Li, Xinyi Li

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 5, 2024

Abstract The medicinal plant Stephania yunnanensis is rich in aporphine alkaloids, a type of benzylisoquinoline alkaloids (BIAs), with being the representative and most abundant compound, but our understanding on biosynthesis BIA this have been relatively limited. Previous research has reported genome S. preliminarily identified upstream gene norcoclaurine synthase (NCS) biosynthetic pathways. However, key genes promoting formation skeleton not yet reported. In study, based differences content crebanine several other BIAs different tissues, we conducted transcriptome sequencing roots, stems, leaves. We then candidate through functional annotation sequence alignment, followed by transcriptomic genomic analyses. Based analysis, three CYP80 enzymes (SyCYP80Q5-1, SyCYP80Q5-3, SyCYP80G6), which exhibited activities towards (S)- (R)-configured substrates yunnanensisand demonstrated strict stereoselectivity enroute to aporphine. This study provides metabolomic information offers valuable insights into elucidation biosynthesis, lays foundation for complete analysis pathways more alkaloids.

Язык: Английский

CRISPR/Cas genome editing for cotton precision breeding: mechanisms, advances, and prospects DOI Creative Commons
Vijay Sheri, M. Harikrishnan, Phanikanth Jogam

и другие.

Journal of Cotton Research, Год журнала: 2025, Номер 8(1)

Опубликована: Фев. 3, 2025

Abstract Cotton ( Gossypium hirsutum L.) is one of the most important global crops that supports textile industry and provides a living for millions farmers. The constantly increasing demand needs significant rise in cotton production. Genome editing technology, specifically with clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) tools, has opened new possibilities trait development cotton. It allows precise efficient manipulation within genome when compared other genetic engineering tools. Current developments CRISPR/Cas including prime editing, base multiplexing have expanded scope traits breeding can be targeted. been employed to generate effectively CRISPRized plants enhanced agronomic traits, fiber yield quality, oil improvement, stress resistance, nutrition. Here we summarized various target genes which successfully altered However, some challenges remain, tetraploid having redundant gene sets homologs making editing. To ensure specificity avoiding off-target effects, need optimize parameters such as site, guide RNA design, choosing right Cas variants. We outline future prospects breeding, suggesting areas further research innovation. A combination speed might useful fastening potentials create customized cultivars meet higher demands agriculture industry.

Язык: Английский

Процитировано

2
Genome-wide identification of CA genes in cotton and the functional analysis of GhαCA4-D, GhβCA6-D and GhγCA2-D in response to drought and salt stresses DOI

Runrun Sun,

Yuanyuan Wang, Runbang Zhu

и другие.

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 140872 - 140872

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

2
Integrated genomic and transcriptomic analyses reveal the genetic and molecular mechanisms underlying hawthorn peel color and seed hardness diversity DOI
Jiaxin Meng, Yan Wang,

Rongkun Guo

и другие.

Journal of genetics and genomics/Journal of Genetics and Genomics, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

1
Antioxidation Activity Enhancement by Intramolecular Hydrogen Bond and Non-Browning Mechanism of Active Ingredients in Rosemary: Carnosic Acid and Carnosol DOI
Xinyu Liu, Wenzhu Wang,

Song‐Po Yao

и другие.

The Journal of Physical Chemistry B, Год журнала: 2024, Номер 128(31), С. 7627 - 7638

Опубликована: Июль 29, 2024

Rosemary is one of the most promising, versatile, and studied natural preservatives. Carnosic acid (CA) carnosol (CARN), as primary active ingredients rosemary extracts, have little difference in structure, but their antioxidant activities vary significantly, depending on system studied. The underlying molecular mechanisms remain unclear. By means optical spectroscopies, stopped-flow, laser photolysis, density functional theory (DFT) calculations, we compared CA CARN between reaction dynamics radical scavenging, metal ion chelation, oxidation inhibition lipid emulsion beef, well interactions with β-carotene (β-Car). For reference, 3-isopropyl catechol (IC), which structurally similar to groups CARN, was parallel. It found for that intramolecular hydrogen bond can boost acidity its phenol hydroxyl synergistic effect β-Car substantially enhance antioxidation activity model systems meat via CA-to-β-Car electron transfer reaction. substitution A B rings group both limits browning caused by formation oxidative products antioxidants.

Язык: Английский

Процитировано

5
Transcriptome sequencing of medical herb Salvia Rosmarinus (Rosemary) revealed the phenylpropanoid biosynthesis pathway genes and their phylogenetic relationships DOI
Dhananjay Singh, Nishu Mittal,

Pooja Mittal

и другие.

Molecular Biology Reports, Год журнала: 2024, Номер 51(1)

Опубликована: Июнь 14, 2024

Язык: Английский

Процитировано

3
Transcriptome Analysis of Stephania yunnanensis and Functional Validation of CYP80s Involved in Benzylisoquinoline Alkaloid Biosynthesis DOI Creative Commons

Wenlong Shi,

Qishuang Li, Xinyi Li

и другие.

Molecules, Год журнала: 2025, Номер 30(2), С. 259 - 259

Опубликована: Янв. 10, 2025

The medicinal plant Stephania yunnanensis is rich in aporphine alkaloids, a type of benzylisoquinoline alkaloid (BIA), with being the representative and most abundant compound, but our understanding biosynthesis BIAs this has been relatively limited. Previous research reported genome S. preliminarily identified norcoclaurine synthase (NCS), which involved early stages BIA biosynthetic pathways. However, key genes promoting formation skeleton have not yet reported. In study, based on differences content crebanine several other different tissues, we conducted transcriptome sequencing roots, stems, leaves. We then candidate through functional annotation sequence alignment further analyzed them combination genome. Based analysis, three CYP80 enzymes (SyCYP80Q5-1, SyCYP80Q5-3, SyCYP80G6), exhibited activities toward (S)- (R)-configured substrates demonstrated strict stereoselectivity enroute to aporphine. This study provides metabolomic transcriptomic information yunnanensis, offers valuable insights into elucidation biosynthesis, lays foundation for complete analysis pathways more alkaloids.

Язык: Английский

Процитировано

0
Integrative omics reveals mechanisms of biosynthesis and regulation of floral scent in Cymbidium tracyanum DOI Creative Commons
Mengling Tu,

Ningyawen Liu,

Zhengshan He

и другие.

Plant Biotechnology Journal, Год журнала: 2025, Номер unknown

Опубликована: Март 17, 2025

Summary Flower scent is a crucial determiner in pollinator attraction and significant horticultural trait ornamental plants. Orchids, which have long been of interest evolutionary biology horticulture, exhibit remarkable diversity floral type intensity. However, the mechanisms underlying biosynthesis regulation orchids remain largely unexplored. In this study, we focus on Cymbidium tracyanum , wild species known for its strong fragrance as primary breeding parent commercial hybrids. We present chromosome‐level genome assembly C. totaling 3.79 Gb size. Comparative genomic analyses reveal expansion gene families associated with terpenoid related metabolic pathways . Integrative analysis genomic, volatolomic transcriptomic data identified terpenoids predominant volatile components flowers characterized spatiotemporal patterns these volatiles CtTPS genes responsible biosynthesis, validating their catalytic functions vitro Dual‐luciferase reporter assays, yeast one‐hybrid assays EMSA experiments confirmed that CtTPS2 CtTPS3, CtTPS8 could be activated by various transcription factors (i.e., CtAP2/ERF1, CtbZIP1, CtMYB2, CtMYB3 CtAP2/ERF4), thereby regulating production corresponding monoterpenes sesquiterpenes. Our study elucidates biosynthetic regulatory great significance fragrant varieties understanding ecological adaptability orchids. This also highlights importance integrating multi‐omics deciphering key traits

Язык: Английский

Процитировано

0
Whole-Genome Sequencing of Flammulina filiformis and Multi-Omics Analysis in Response to Low Temperature DOI Creative Commons
Xuanqiang Liang, Jing Han,

Yuqin Cui

и другие.

Journal of Fungi, Год журнала: 2025, Номер 11(3), С. 229 - 229

Опубликована: Март 17, 2025

The growth of Flammulina filiformis is strongly dependent on low-temperature cues for the initiation primordia formation. To obtain a comprehensive understanding molecular mechanisms that govern mycelial response to cold stress, de novo genome sequencing F. monokaryon and multi-omics data (transcriptome metabolome) analyses mycelia, primordia, fruiting bodies were conducted in present study. Genome based PacBio HiFi Hi-C resulted 36.3 Mb sequence mapped 12 chromosomes, comprising 11,886 protein-coding genes. A total 25 cold-responsive (COR) genes 520 cold-adapted enzymes identified genome. Multi-omics showed pathways related carbohydrate metabolism mycelia under low temperature (10 °C) significantly enriched. Further examination expression profiles carbohydrate-active (CAZymes) involved revealed out 515 CAZyme filiformis, 58 specifically upregulated conditions. By contrast, levels these reverted those prior exposure. These indicate are important adaptation filiformis. This research contributes targeted breeding

Язык: Английский

Процитировано

0
Genomic research and genetic improvement of orphan crops: novel strategies for addressing global food security challenges DOI
Zijun Wang, Guisheng Xiang

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Oxidative Stress in Rice (Oryza sativa): Mechanisms, Impact, and Adaptive Strategies DOI Creative Commons

Lady Edlenill J. Tavu,

Mark Christian Felipe R. Redillas

Plants, Год журнала: 2025, Номер 14(10), С. 1463 - 1463

Опубликована: Май 14, 2025

Oxidative stress, arising from environmental challenges such as drought, salinity, extreme temperatures, and pathogen attack, significantly impairs rice (Oryza sativa) growth, yield, grain quality. This review provides a comprehensive synthesis of the mechanisms underlying oxidative stress in rice, with focus on generation reactive oxygen species (ROS), their physiological molecular impacts, antioxidant defense systems employed for mitigation. The roles enzymatic non-enzymatic antioxidants, along key transcription factors, signaling pathways, stress-responsive genes, are explored detail. study further highlights varietal differences tolerance, emphasizing traditional, modern, genetically engineered cultivars. Recent advances breeding strategies, gene editing technologies, multi-omics integration discussed promising approaches enhancing resilience. regulatory influence epigenetic modifications small RNAs modulating responses is also examined. Finally, this paper identifies critical research gaps-including need multi-stress long-term field validation, deeper insights into non-coding RNA functions-and offers recommendations to inform development climate-resilient varieties through integrative, sustainable strategies.

Язык: Английский

Процитировано

0