Research Square (Research Square),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 5, 2024
Abstract
The
medicinal
plant
Stephania
yunnanensis
is
rich
in
aporphine
alkaloids,
a
type
of
benzylisoquinoline
alkaloids
(BIAs),
with
being
the
representative
and
most
abundant
compound,
but
our
understanding
on
biosynthesis
BIA
this
have
been
relatively
limited.
Previous
research
has
reported
genome
S.
preliminarily
identified
upstream
gene
norcoclaurine
synthase
(NCS)
biosynthetic
pathways.
However,
key
genes
promoting
formation
skeleton
not
yet
reported.
In
study,
based
differences
content
crebanine
several
other
BIAs
different
tissues,
we
conducted
transcriptome
sequencing
roots,
stems,
leaves.
We
then
candidate
through
functional
annotation
sequence
alignment,
followed
by
transcriptomic
genomic
analyses.
Based
analysis,
three
CYP80
enzymes
(SyCYP80Q5-1,
SyCYP80Q5-3,
SyCYP80G6),
which
exhibited
activities
towards
(S)-
(R)-configured
substrates
yunnanensisand
demonstrated
strict
stereoselectivity
enroute
to
aporphine.
This
study
provides
metabolomic
information
offers
valuable
insights
into
elucidation
biosynthesis,
lays
foundation
for
complete
analysis
pathways
more
alkaloids.
Journal of Cotton Research,
Journal Year:
2025,
Volume and Issue:
8(1)
Published: Feb. 3, 2025
Abstract
Cotton
(
Gossypium
hirsutum
L.)
is
one
of
the
most
important
global
crops
that
supports
textile
industry
and
provides
a
living
for
millions
farmers.
The
constantly
increasing
demand
needs
significant
rise
in
cotton
production.
Genome
editing
technology,
specifically
with
clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)/CRISPR-associated
protein
(Cas)
tools,
has
opened
new
possibilities
trait
development
cotton.
It
allows
precise
efficient
manipulation
within
genome
when
compared
other
genetic
engineering
tools.
Current
developments
CRISPR/Cas
including
prime
editing,
base
multiplexing
have
expanded
scope
traits
breeding
can
be
targeted.
been
employed
to
generate
effectively
CRISPRized
plants
enhanced
agronomic
traits,
fiber
yield
quality,
oil
improvement,
stress
resistance,
nutrition.
Here
we
summarized
various
target
genes
which
successfully
altered
However,
some
challenges
remain,
tetraploid
having
redundant
gene
sets
homologs
making
editing.
To
ensure
specificity
avoiding
off-target
effects,
need
optimize
parameters
such
as
site,
guide
RNA
design,
choosing
right
Cas
variants.
We
outline
future
prospects
breeding,
suggesting
areas
further
research
innovation.
A
combination
speed
might
useful
fastening
potentials
create
customized
cultivars
meet
higher
demands
agriculture
industry.
The Journal of Physical Chemistry B,
Journal Year:
2024,
Volume and Issue:
128(31), P. 7627 - 7638
Published: July 29, 2024
Rosemary
is
one
of
the
most
promising,
versatile,
and
studied
natural
preservatives.
Carnosic
acid
(CA)
carnosol
(CARN),
as
primary
active
ingredients
rosemary
extracts,
have
little
difference
in
structure,
but
their
antioxidant
activities
vary
significantly,
depending
on
system
studied.
The
underlying
molecular
mechanisms
remain
unclear.
By
means
optical
spectroscopies,
stopped-flow,
laser
photolysis,
density
functional
theory
(DFT)
calculations,
we
compared
CA
CARN
between
reaction
dynamics
radical
scavenging,
metal
ion
chelation,
oxidation
inhibition
lipid
emulsion
beef,
well
interactions
with
β-carotene
(β-Car).
For
reference,
3-isopropyl
catechol
(IC),
which
structurally
similar
to
groups
CARN,
was
parallel.
It
found
for
that
intramolecular
hydrogen
bond
can
boost
acidity
its
phenol
hydroxyl
synergistic
effect
β-Car
substantially
enhance
antioxidation
activity
model
systems
meat
via
CA-to-β-Car
electron
transfer
reaction.
substitution
A
B
rings
group
both
limits
browning
caused
by
formation
oxidative
products
antioxidants.
Molecules,
Journal Year:
2025,
Volume and Issue:
30(2), P. 259 - 259
Published: Jan. 10, 2025
The
medicinal
plant
Stephania
yunnanensis
is
rich
in
aporphine
alkaloids,
a
type
of
benzylisoquinoline
alkaloid
(BIA),
with
being
the
representative
and
most
abundant
compound,
but
our
understanding
biosynthesis
BIAs
this
has
been
relatively
limited.
Previous
research
reported
genome
S.
preliminarily
identified
norcoclaurine
synthase
(NCS),
which
involved
early
stages
BIA
biosynthetic
pathways.
However,
key
genes
promoting
formation
skeleton
have
not
yet
reported.
In
study,
based
on
differences
content
crebanine
several
other
different
tissues,
we
conducted
transcriptome
sequencing
roots,
stems,
leaves.
We
then
candidate
through
functional
annotation
sequence
alignment
further
analyzed
them
combination
genome.
Based
analysis,
three
CYP80
enzymes
(SyCYP80Q5-1,
SyCYP80Q5-3,
SyCYP80G6),
exhibited
activities
toward
(S)-
(R)-configured
substrates
demonstrated
strict
stereoselectivity
enroute
to
aporphine.
This
study
provides
metabolomic
transcriptomic
information
yunnanensis,
offers
valuable
insights
into
elucidation
biosynthesis,
lays
foundation
for
complete
analysis
pathways
more
alkaloids.
Plant Biotechnology Journal,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 17, 2025
Summary
Flower
scent
is
a
crucial
determiner
in
pollinator
attraction
and
significant
horticultural
trait
ornamental
plants.
Orchids,
which
have
long
been
of
interest
evolutionary
biology
horticulture,
exhibit
remarkable
diversity
floral
type
intensity.
However,
the
mechanisms
underlying
biosynthesis
regulation
orchids
remain
largely
unexplored.
In
this
study,
we
focus
on
Cymbidium
tracyanum
,
wild
species
known
for
its
strong
fragrance
as
primary
breeding
parent
commercial
hybrids.
We
present
chromosome‐level
genome
assembly
C.
totaling
3.79
Gb
size.
Comparative
genomic
analyses
reveal
expansion
gene
families
associated
with
terpenoid
related
metabolic
pathways
.
Integrative
analysis
genomic,
volatolomic
transcriptomic
data
identified
terpenoids
predominant
volatile
components
flowers
characterized
spatiotemporal
patterns
these
volatiles
CtTPS
genes
responsible
biosynthesis,
validating
their
catalytic
functions
vitro
Dual‐luciferase
reporter
assays,
yeast
one‐hybrid
assays
EMSA
experiments
confirmed
that
CtTPS2
CtTPS3,
CtTPS8
could
be
activated
by
various
transcription
factors
(i.e.,
CtAP2/ERF1,
CtbZIP1,
CtMYB2,
CtMYB3
CtAP2/ERF4),
thereby
regulating
production
corresponding
monoterpenes
sesquiterpenes.
Our
study
elucidates
biosynthetic
regulatory
great
significance
fragrant
varieties
understanding
ecological
adaptability
orchids.
This
also
highlights
importance
integrating
multi‐omics
deciphering
key
traits
Journal of Fungi,
Journal Year:
2025,
Volume and Issue:
11(3), P. 229 - 229
Published: March 17, 2025
The
growth
of
Flammulina
filiformis
is
strongly
dependent
on
low-temperature
cues
for
the
initiation
primordia
formation.
To
obtain
a
comprehensive
understanding
molecular
mechanisms
that
govern
mycelial
response
to
cold
stress,
de
novo
genome
sequencing
F.
monokaryon
and
multi-omics
data
(transcriptome
metabolome)
analyses
mycelia,
primordia,
fruiting
bodies
were
conducted
in
present
study.
Genome
based
PacBio
HiFi
Hi-C
resulted
36.3
Mb
sequence
mapped
12
chromosomes,
comprising
11,886
protein-coding
genes.
A
total
25
cold-responsive
(COR)
genes
520
cold-adapted
enzymes
identified
genome.
Multi-omics
showed
pathways
related
carbohydrate
metabolism
mycelia
under
low
temperature
(10
°C)
significantly
enriched.
Further
examination
expression
profiles
carbohydrate-active
(CAZymes)
involved
revealed
out
515
CAZyme
filiformis,
58
specifically
upregulated
conditions.
By
contrast,
levels
these
reverted
those
prior
exposure.
These
indicate
are
important
adaptation
filiformis.
This
research
contributes
targeted
breeding
