Glume-opening
of
thermosensitive
genic
male
sterile
(TGMS)
rice
(Oryza
sativa
L.)
lines
after
anthesis
is
a
serious
problem
that
significantly
reduces
the
yield
and
quality
hybrid
seeds.
However,
molecular
mechanisms
regulating
opening
closing
glumes
remain
largely
unclear.
In
this
study,
we
report
isolation
functional
characterization
glum-opening
mutant
anthesis,
named
gom1.
gom1
exhibits
dysfunctional
lodicules
lead
to
open
following
anthesis.
Map-based
cloning
subsequent
complementation
tests
confirmed
GOM1
encodes
receptor-like
kinase
(RLK).
was
expressed
in
nearly
all
floral
tissues,
with
highest
expression
lodicule.
Loss-of-function
resulted
decrease
genes
related
JA
biosynthesis,
signaling,
sugar
transport.
Compared
LK638S,
content
reduced,
while
soluble
sugar,
sucrose,
glucose,
fructose
contents
were
increased
Together,
speculated
regulates
carbohydrate
transport
during
through
maintaining
higher
osmolality
which
leads
glum-opening.
The Plant Journal,
Год журнала:
2024,
Номер
119(6), С. 2585 - 2598
Опубликована: Июль 7, 2024
Diurnal
flower-opening
time
(DFOT),
the
of
spikelet
opening
during
day,
is
an
important
trait
for
hybrid
rice
(Oryza
sativa
L.)
seed
production.
Hybrids
between
indica
and
japonica
varieties
have
strong
heterosis,
but
parental
lines
usually
different,
nonoverlapping
DFOTs.
This
reduces
success
production
in
crosses
subspecies,
thus
hindering
utilization
inter-subspecies
heterosis.
However,
little
known
about
molecular
mechanisms
regulating
DFOT
rice.
Here,
we
obtained
with
a
1.5
h
earlier
than
wild
type
by
overexpressing
OsMYC2,
gene
encoding
key
transcription
factor
jasmonate
(JA)
signaling
pathway.
OsMYC2
activated
JA
directly
regulates
genes
related
to
biosynthesis
cell
wall
metabolism.
Overexpressing
led
significantly
increased
contents
decreased
cellulose
hemicellulose
lodicule
cells,
as
well
softening
walls.
may
facilitate
swelling
lodicules,
resulting
early
diurnal
flower-opening.
These
results
suggest
that
OsMYC2-JA
feedback
loop
via
remodeling.
findings
shed
light
on
understanding
regulatory
mechanism
plants,
which
should
promote
development
suitable
breeding.
Cell Reports,
Год журнала:
2025,
Номер
44(3), С. 115390 - 115390
Опубликована: Март 1, 2025
SummaryClimate-change-induced
extreme
heat
and
drought
increasingly
threaten
plant
growth
development,
with
a
particularly
significant
impact
on
sexual
reproduction.
Heat
stress
can
disrupt
key
stages
of
reproduction,
including
flowering
time,
gametophyte
pollination,
seed
formation,
leading
to
infertility
substantial
yield
reductions
in
crops.
This
review
systematically
summarizes
the
latest
research
effects
various
reproduction
proposes
specific
strategies
mitigate
agricultural
hazards
posed
by
these
stresses.
By
providing
an
in-depth
analysis
underlying
mechanisms
regulatory
networks,
this
offers
theoretical
basis
for
advancing
fundamental
optimizing
practices
address
severe
challenges
climate
change
presents
agriculture.Graphical
abstract
New Phytologist,
Год журнала:
2024,
Номер
244(1), С. 176 - 191
Опубликована: Авг. 12, 2024
Diurnal
floret
opening
time
(DFOT)
is
a
pivotal
trait
for
successful
fertilization
and
hybrid
breeding
in
rice.
However,
the
molecular
mechanism
underlying
this
poorly
understood
In
study,
we
combined
cytological,
genetic
studies
to
demonstrate
that
jasmonic
acid
(JA)
regulates
DFOT
rice
through
modulating
turgor
osmotic
pressure
of
lodicules.
We
show
lodicules
undergo
dramatic
morphologic
changes,
accompanied
by
changes
water
sugar
contents
during
process
opening.
Consistently,
large
set
genes
associated
with
cell
osmolality
wall
remodeling
exhibits
distinct
expression
profiles
at
different
points
our
time-course
transcriptomes
Notably,
group
JA
biosynthesis
signaling
continuously
upregulated,
gradual
increase
accumulation
as
approaching.
Furthermore,
gene
OsAOS1
required
endogenous
promoting
DFOT.
Moreover,
OsMYC2,
master
regulator
signaling,
directly
activating
OsAOS1,
OsSWEET4,
OsPIP2;2
OsXTH9.
Collectively,
findings
establish
core
regulatory
network
mediated
provide
effective
targets
improvement
Plant Molecular Biology,
Год журнала:
2025,
Номер
115(1)
Опубликована: Янв. 17, 2025
Various
biological
processes
are
interconnected
in
plants.
Transcription
factors
(TFs)
often
act
as
regulatory
hubs
to
regulate
plant
growth
and
responses
stress
by
integrating
various
pathways.
Despite
extensive
studies
on
TFs
functions
species,
our
understanding
of
the
details
regulation
remains
limited.
In
this
study,
clonal
seedlings
Salvia
miltiorrhiza
were
exposed
specific
inhibitors
for
12
h.
Time-series
transcriptome
data,
sampled
hourly,
used
construct
co-expression
networks
gene
(GRNs).
Transcriptome
dynamic
analysis
was
utilized
capture
expression
dynamics
decipher
potential
molecular
mechanisms
that
these
processes.
The
perturbation
results
showed
development
S.miltiorrhiza
primarily
affected
at
early
stage,
whereas
response-related
mainly
influenced
later
stage.
And
there
a
correlation
between
series
key
differentially
expressed
genes
terpenoid
biosynthesis
pathways
topological
distribution
Furthermore,
GRNs
based
indicate
play
crucial
role
connecting
cytoplasmic
lysate
module,
SmWRKY48-SmTCP4-SmWRKY28
constituted
hub
regulating
MVA
pathway.
mediated
pathways,
including
pyruvate
metabolism,
glycolysis/gluconeogenesis,
amino
acid
ubiquinone
other
terpenoid-quinone
biosynthesis.Our
findings
suggest
pathway
has
time-dependent
effects
constitutes
After
disrupting
with
inhibitors,
network
involving
transcription
reveals
mechanism
synergistic
