Israel Journal of Chemistry,
Год журнала:
2024,
Номер
64(5)
Опубликована: Май 1, 2024
Abstract
N
6
‐methyladenosine
(m
A),
as
the
most
abundant
and
well‐studied
RNA
modification,
can
be
reversibly
added
or
removed
by
m
A
methyltransferase
demethylase.
The
further
molecular
biological
function
of
is
achieved
recognition
its
binding
protein.
functions
in
diverse
progress
processing,
including
transcription
regulation,
splicing,
nuclear
export,
stability,
translation,
to
regulate
fate
cells.
Although
been
extensively
studied
various
animal
cell
systems,
research
on
A's
regulatory
plant
cells
lags.
In
recent
years,
with
a
deepening
understanding
development
sequencing
technologies,
researches
have
gradually
increased.
this
review,
we
focused
discussing
nucleus
cytoplasm,
aiming
elucidate
specific
mechanisms
which
regulates
RNAs
plants.
Finally,
provide
some
perspectives
future
investigations
detailed
mechanism
A‐mediated
regulation
plants,
might
insights
into
strategies
for
achieving
multiple
growth
processes
crops.
Plant Communications,
Год журнала:
2024,
Номер
5(10), С. 101037 - 101037
Опубликована: Июль 6, 2024
N6-methyladenosine
(m6A)
is
the
most
abundant
modification
observed
in
eukaryotic
mRNAs.
Advances
transcriptome-wide
m6A
mapping
and
sequencing
technologies
have
enabled
identification
of
several
conserved
motifs
plants,
including
RRACH
(R
=
A/G
H
A/C/U)
UGUAW
(W
U
or
A)
motifs.
However,
mechanisms
underlying
deposition
marks
at
specific
positions
individual
transcripts
remain
to
be
clarified.
Evidence
from
plant
animal
studies
suggests
that
writer
eraser
components
are
recruited
genomic
loci
through
interactions
with
particular
transcription
factors,
5-methylcytosine
DNA
methylation
marks,
histone
marks.
In
addition,
recent
cells
shown
microRNAs
play
a
role
depositing
sites
base-pairing
mechanism.
also
affects
biogenesis
function
chromatin-associated
regulatory
RNAs
long
noncoding
RNAs.
Although
we
less
an
understanding
link
between
epigenetic
factors
plants
than
animals,
progress
identifying
proteins
interact
has
provided
insights
into
crosstalk
which
plays
crucial
transcript-specific
regulation
plants.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Июнь 7, 2024
Abstract
N
6
-methyladenosine
(m
A)
plays
critical
roles
in
regulating
mRNA
metabolism.
However,
comprehensive
m
A
methylomes
different
plant
tissues
with
single-base
precision
have
yet
to
be
reported.
Here,
we
present
transcriptome-wide
maps
at
resolution
of
rice
and
Arabidopsis
using
A-SAC-seq.
Our
analysis
uncovers
a
total
205,691
sites
distributed
across
22,574
genes
rice,
188,282
19,984
.
The
evolutionarily
conserved
ortholog
gene
pairs
are
involved
controlling
tissue
development,
photosynthesis
stress
response.
We
observe
an
overall
stabilization
effect
by
3’
UTR
certain
tissues.
Like
mammals,
positive
correlation
between
the
level
length
internal
exons
is
also
observed
mRNA,
except
for
last
exon.
data
suggest
active
deposition
process
occurring
near
stop
codon
mRNA.
In
addition,
MTA-installed
correlate
both
translation
promotion
suppression,
depicting
more
complicated
regulatory
picture.
results
therefore
provide
in-depth
resources
relating
functions
plants
uncover
suppression-activation
model
biogenesis
species.
Over
the
past
decade,
N
6
-methyladenosine
(m
A)
has
emerged
as
a
prevalent
and
dynamically
regulated
modification
across
transcriptome;
it
been
reversibly
installed,
removed,
interpreted
by
specific
binding
proteins,
played
crucial
roles
in
molecular
biological
processes.
Within
this
scope,
we
consolidate
recent
advancements
of
m
A
research
plants
regarding
gene
expression
regulation,
diverse
physiologic
pathogenic
processes,
well
crop
trial
implications,
to
guide
discussions
on
challenges
associated
with
leveraging
epitranscriptome
editing
for
improvement.
Journal of Integrative Plant Biology,
Год журнала:
2024,
Номер
66(12), С. 2586 - 2599
Опубликована: Окт. 4, 2024
ABSTRACT
Modifications
to
RNA
have
recently
been
recognized
as
a
pivotal
regulator
of
gene
expression
in
living
organisms.
More
than
170
chemical
modifications
identified
RNAs,
with
N
6
‐methyladenosine
(m
A)
being
the
most
abundant
modification
eukaryotic
mRNAs.
The
addition
and
removal
m
A
marks
are
catalyzed
by
methyltransferases
(referred
“writers”)
demethylases
“erasers”),
respectively.
In
addition,
mRNAs
interpreted
A‐binding
proteins
“readers”),
which
regulate
fate
mRNAs,
including
stability,
splicing,
transport,
translation.
Therefore,
exploring
mechanism
underlying
reader‐mediated
modulation
metabolism
is
essential
for
much
deeper
understanding
epigenetic
role
plants.
Recent
discoveries
improved
our
functions
readers
plant
growth
development,
stress
response,
disease
resistance.
This
review
highlights
latest
developments
reader
research,
emphasizing
diverse
RNA‐binding
domains
crucial
function
biological
cellular
roles
response
developmental
environmental
signals.
Moreover,
we
propose
discuss
potential
future
research
directions
challenges
identifying
novel
elucidating
mechanistic
Molecular Ecology,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 5, 2024
ABSTRACT
Genetic
mechanisms
have
been
at
the
forefront
of
our
exploration
into
substrate
adaptive
evolution
and
phenotypic
diversification.
However,
genetic
variation
only
accounts
for
a
fraction
variation.
In
last
decade,
significance
RNA
modification
has
become
more
apparent
in
context
organismal
adaptation
to
rapidly
changing
environments.
m
6
A
methylation,
most
abundant
form
modification,
is
emerging
as
potentially
significant
player
various
biological
processes.
Despite
its
fundamental
function
regulate
other
major
post‐transcriptional
such
microRNA
alternative
splicing,
role
ecology
understudied.
This
review
highlights
potential
importance
methylation
ecological
adaptation,
emphasising
need
further
research,
especially
natural
systems.
We
focus
on
how
not
affects
mRNA
fate
but
also
influences
miRNA‐mediated
gene
regulation
contributing
adaptation.
The
aim
this
synthesise
key
background
information
enhance
understanding
driving
species
survival
dynamic
environments
motivate
future
research
dynamics
methylation.
Frontiers in Plant Science,
Год журнала:
2024,
Номер
15
Опубликована: Июль 16, 2024
The
epitranscriptomic
mark
N
6-methyladenosine
(m6A)
is
the
most
common
type
of
messenger
RNA
(mRNA)
post-transcriptional
modification
in
eukaryotes.
With
discovery
demethylase
FTO
(FAT
MASS
AND
OBESITY-ASSOCIATED
PROTEIN)
Homo
Sapiens,
this
has
been
proven
to
be
dynamically
reversible.
technological
advances,
research
on
m6A
plants
also
rapidly
developed.
widely
distributed
plants,
which
usually
enriched
near
stop
codons
and
3'-UTRs,
conserved
sequences.
related
proteins
mainly
consist
three
components:
methyltransferases
(writers),
demethylases
(erasers),
reading
(readers).
regulates
growth
development
by
modulating
metabolic
processes
playing
an
important
role
their
responses
environmental
signals.
In
review,
we
briefly
outline
detection
techniques;
comparatively
analyze
distribution
characteristics
plants;
summarize
methyltransferases,
demethylases,
binding
m6A;
elaborate
how
functions
plant
growth,
development,
response
signals;
provide
a
summary
outlook
plants.
Summary
Among
many
mRNA
modifications,
adenine
methylation
at
the
N
6
position
(N
‐methyladenosine,
m
A)
is
known
to
affect
biology
extensively.
The
influence
of
A
has
yet
be
assessed
under
drought,
one
most
impactful
abiotic
stresses.
We
show
that
Arabidopsis
thaliana
(L.)
Heynh.
(Arabidopsis)
plants
lacking
ADENOSINE
METHYLASE
(MTA)
are
drought‐sensitive.
Subsequently,
we
comprehensively
assess
impacts
MTA‐dependent
changes
during
drought
on
abundance,
stability,
and
translation
in
Arabidopsis.
During
there
a
global
trend
toward
hypermethylation
protein‐coding
transcripts
does
not
occur
mta
.
also
observe
complex
regulation
transcript‐specific
level,
possibly
reflecting
compensation
by
other
components.
Importantly,
subset
hypermethylated
an
manner
exhibited
reduced
turnover
,
compared
with
wild‐type
(WT)
plants,
drought.
Additionally,
MTA
transcript
stability
independently
A.
correlate
drought‐associated
deposition
increased
modulators
response,
such
as
RD29A
COR47
COR413
ALDH2B
ERD7
ABF4
WT,
which
impaired
dynamic
and,
alongside
MTA,
promotes
tolerance
regulating
drought‐responsive
translation.
Physiologia Plantarum,
Год журнала:
2025,
Номер
177(1)
Опубликована: Янв. 1, 2025
Abstract
N6‐methyladenosine
(m
6
A),
the
most
prevalent
modification
found
in
eukaryotic
mRNAs,
is
recognized
and
interpreted
by
m
A‐binding
proteins
called
A
readers.
The
EVOLUTIONARILY
CONSERVED
C‐TERMINAL
REGION
(ECT)
have
increasingly
been
identified
as
readers
plants.
recent
study
has
demonstrated
that
loss‐of‐function
ect8
mutant
sensitive
to
salt
stress
enhancing
stability
of
negative
regulators
Arabidopsis
(
thaliana
).
In
this
study,
we
generated
analyzed
ECT8‐overexpressing
transgenic
plants
further
explore
function
ECT8
response.
electrophoretic
mobility
shift
assay
vitro
showed
binds
A‐modified
synthetic
RNAs,
preferring
UGUm
AA
UACm
AGA
motifs
over
GGm
ACU
motif.
Contrary
exhibiting
hypersensitivity
regulators,
displayed
tolerance
increasing
expression
levels
positive
regulators.
Moreover,
RNA‐immunoprecipitation
stress‐responsive
mRNAs
planta
.
Collectively,
our
current
previous
findings
highlight
ECT8‐mediated
stabilization
destabilization
genes
encoding
or
respectively,
contribute
Arabidopsis.
