Plant specialized metabolism: Diversity of terpene synthases and their products

Current Opinion in Plant Biology, Год журнала: 2024, Номер 81, С. 102607 - 102607

Опубликована: Июль 24, 2024

Язык: Английский

---

Impact of plant monoterpenes on insect pest management and insect-associated microbes

Heliyon, Год журнала: 2024, Номер 10(20), С. e39120 - e39120

Опубликована: Окт. 1, 2024

The fight against insect pests primarily relies on the utilization of synthetic insecticides. However, improper application these chemicals can lead to detrimental effects both environment and human health, as well foster development resistance. Consequently, novel strategies must be implemented address challenges stemming from prolonged use insecticides in agricultural public health environments. Certain involve combination crop protectants, which not only enhance insecticidal effectiveness but also reduce rates. Plant-based natural products emerge promising alternatives for management. Monoterpenes, are abundant plant compounds produced through activation various enzymes, have attracted significant attention their control. Notably, they prolific fragrance-producing plants. This review explores defense, insecticidal, antimicrobial characteristics monoterpenes pests, shedding light potential modes action possibilities commercialization. Emphasizing role targeted environmentally safer, highlights practical viability within integrated pest management programs.

Язык: Английский

---

Immunity-associated volatile emissions of β-ionone and nonanal propagate defence responses in neighbouring barley plants

Journal of Experimental Botany, Год журнала: 2021, Номер 73(2), С. 615 - 630

Опубликована: Ноя. 24, 2021

Plants activate biochemical responses to combat stress. (Hemi-)biotrophic pathogens are fended off by systemic acquired resistance (SAR), a primed state allowing plants respond faster and more strongly upon subsequent infection. Here, we show that SAR-like defences in barley (Hordeum vulgare) propagated between neighbouring plants, which with enhanced the volatile cues from infected senders. The emissions of sender contained 15 organic compounds (VOCs) associated Two these, β-ionone nonanal, elicited plant exposure. Whole-genome transcriptomics analysis confirmed interplant propagation defence is established as form priming. Although gene expression changes were pronounced after challenge infection receiver Blumeria graminis f. sp. hordei, differential response included an induction HISTONE DEACETYLASE 2 (HvHDA2) priming TETRATRICOPEPTIDE REPEAT-LIKE superfamily protein (HvTPL). Because HvHDA2 HvTPL transcript accumulation was also exposure our data identify both genes possible defence/priming markers barley. Our results suggest VOCs plant-plant interactions relevant for crop protection strategies

Язык: Английский

---

Terpenoid Transport in Plants: How Far from the Final Picture?

Plants, Год журнала: 2023, Номер 12(3), С. 634 - 634

Опубликована: Фев. 1, 2023

Contrary to the biosynthetic pathways of many terpenoids, which are well characterized and elucidated, their transport inside subcellular compartments secretion reaction intermediates final products at short- (cell-to-cell), medium- (tissue-to-tissue), long-distance (organ-to-organ) levels still poorly understood, with some limited exceptions. In this review, we aim describe state art several terpene classes that have important physiological ecological roles or represent high-value bioactive molecules. Among tens thousands terpenoids identified in plant kingdom, only less than 20 been from point view localization. Most secreted apoplast stored vacuoles by action ATP-binding cassette (ABC) transporters. However, little information is available regarding movement terpenoid plastids endoplasmic reticulum cytosol. Through a description mechanisms cytosol- plastid-synthesized terpenes, attempt provide hypotheses, suggestions, general schemes about trafficking different substrates, intermediates, products, might help develop novel strategies approaches allow for future identification transporters uncharacterized.

Язык: Английский

---

The behavioral sensitivity of mice to acyclic, monocyclic, and bicyclic monoterpenes

PLoS ONE, Год журнала: 2024, Номер 19(2), С. e0298448 - e0298448

Опубликована: Фев. 23, 2024

Monoterpenes are a large class of naturally occurring fragrant molecules. These chemicals commonly used in olfactory studies to survey neural activity and probe the behavioral limits odor discrimination. (typically form essential oils) have been for centuries therapeutic purposes pivotal roles various biological medical applications. Despite their importance multiple lines research using rodent models role system detecting these volatile chemicals, murine sensitivity monoterpenes remains mostly unexplored. We assayed ability C57BL/6J mice detect nine different (the acyclic monoterpenes: geraniol, citral, linalool; monocyclic r-limonene, s-limonene, γ-terpinene; bicyclic eucalyptol, α-pinene, β-pinene) head-fixed Go / No-Go operant conditioning assay. found that can reliably monoterpene concentrations low parts per billion ( ppb ) range. Specifically, were most sensitive geraniol (threshold: 0.7 ppb) least γ-terpinene 18.1 ppb). estimations serve set lower limit relevant functional experiments mice. To define an upper limit, we estimated maximum mouse may experience nature by collating published headspace analyses emitted from natural sources. typically ranged ~1 1000 ppb. It is our hope this dataset will help researchers use appropriate provide context vapor-phase delivery investigating

Язык: Английский

---

Interorgan, intraorgan and interplant communication mediated by nitric oxide and related species

New Phytologist, Год журнала: 2024, Номер 244(3), С. 786 - 797

Опубликована: Сен. 2, 2024

Summary Plant survival to a potential plethora of diverse environmental insults is underpinned by coordinated communication amongst organs help shape effective responses these challenges at the whole plant level. This interorgan supported complex signal network that regulates growth, development and responses. Nitric oxide (NO) has emerged as key signalling molecule in plants. However, its role only recently started come into view. Direct indirect evidence supporting NO related species ( S ‐nitrosoglutathione, nitro‐linolenic acid) are mobile signals transmitting stresses such hypoxia heat. Beyond their signals, involved mediating xylem development, thus contributing efficient root–shoot communication. Moreover, regulators intraorgan systemic defence aiming an effective, against pathogens. planta role, may act ex coordinating external leaf‐to‐leaf, root‐to‐leaf but also plant‐to‐plant Here, we discuss exciting developments emphasise how manipulation provide novel strategies for crop improvement.

Язык: Английский

---

The genomic and enzymatic basis for iridoid biosynthesis in cat thyme (Teucrium marum)

The Plant Journal, Год журнала: 2024, Номер 118(5), С. 1589 - 1602

Опубликована: Март 15, 2024

SUMMARY Iridoids are non‐canonical monoterpenoids produced by both insects and plants. An example is the cat‐attracting insect‐repelling volatile iridoid nepetalactone, Nepeta sp. (catmint) aphids. Recently, nepetalactone biosynthetic pathways were elucidated, showing a remarkable convergent evolution. The iridoid, dolichodial, Teucrium marum (cat thyme) multiple insect species, has highly similar properties to but its origin remains unknown. We set out determine genomic, enzymatic, evolutionary basis of biosynthesis in T. . First, we generated de novo chromosome‐scale genome assembly for using Oxford Nanopore Technologies long reads proximity‐by‐ligation Hi‐C reads. 610.3 Mb spans 15 pseudomolecules with 32.9 N50 scaffold size. This enabled identification genes, whose roles verified via activity assays. Phylogenomic analysis revealed that history synthase, scaffold‐forming enzyme, not orthologous typical synthases derived from conserved paralog. discovered an enzymatic route nepetalactol diverse iridoids through coupled oxidase cytochrome P450 acetyltransferases, inferred acylated intermediate. work provides genomic resource specialized metabolite research mints demonstration role acetylation diversity. will enable future biocatalytic or production potent repellents, as well comparative studies into insects.

Язык: Английский

---

The potential of Burkholderia gladioli KRS027 in plant growth promotion and biocontrol against Verticillium dahliae revealed by dual transcriptome of pathogen and host

Microbiological Research, Год журнала: 2024, Номер 287, С. 127836 - 127836

Опубликована: Июль 15, 2024

Verticillium dahliae is a destructive, soil-borne pathogen that causes significant losses on numerous important dicots. Recently, beneficial microbes inhabiting the rhizosphere have been exploited and used to control plant diseases. In present study, Burkholderia gladioli KRS027 demonstrated excellent inhibitory effects against wilt in cotton seedlings. Plant growth development was promoted by affecting biosynthesis signaling pathways of brassinosteroids (BRs), gibberellins (GAs), auxins, consequently promoting stem elongation, shoot apical meristem, root tissue division cotton. Furthermore, based host transcriptional response V. infection, it found modulates plants maintain cell homeostasis respond other stress. Moreover, induced disruption cellular structures, as evidenced scanning electron microscopy (SEM) transmission (TEM) analyses. Based comparative transcriptomic analysis between treated group dahliae, substantial alterations transcriptome, particularly genes encoding secreted proteins, small cysteine-rich proteins (SCRPs), protein kinases. addition, suppressed different clonal lineages strains through metabolites, volatile organic compounds (VOCs) released inhibited melanin microsclerotia development. These findings provide valuable insights into an alternative biocontrol strategy for wilt, demonstrating antagonistic bacterium holds promise agent managing disease occurrence.

Язык: Английский

---

Mycorrhiza-Tree-Herbivore Interactions: Alterations in Poplar Metabolome and Volatilome

Metabolites, Год журнала: 2022, Номер 12(2), С. 93 - 93

Опубликована: Янв. 19, 2022

Plants are continuously interacting with other organisms to optimize their performance in a changing environment. Mycorrhization is known affect the plant growth and nutrient status, but it also can lead adjusted defense alter interactions trophic levels. Here, we studied effect of Laccaria bicolor-mycorrhization on poplar (Populus x canescens) metabolome volatilome trees without leaf beetle (Chrysomela populi) infestation. We analyzed root metabolomes employing liquid chromatography-mass spectrometry, headspace sorptive extraction combined gas-chromatography-mass spectrometry. caused distinct metabolic adjustments roots, young/infested leaves old/not directly infested leaves. lipid composition, abundance peptides and, especially upon herbivory, level various phenolic compounds. The greatest change volatile organic compound (VOC) emissions occurred four eight days following Together, these results prove that mycorrhization affects whole may influence aboveground interactions. herbivores mycorrhizal fungi interact each indirectly through common host plant, result emphasizes importance community approach chemical ecology.

Язык: Английский

---

The biochemical and molecular investigation of flower color and scent sheds lights on further genetic modification of ornamental traits in Clivia miniata

Horticulture Research, Год журнала: 2022, Номер 9

Опубликована: Янв. 5, 2022

Abstract Clivia miniata is renowned for its evergreen and strap-like leaves, whereas floral color scent are lacking diversity. Here, anthocyanin, volatile terpene, carotenoid metabolisms were integrally investigated in C. flowers. The results showed that pelargonidins lutein might cooperate to confer orange or yellow flowers, but only a trace amount of (+)-limonene was detected. expression levels CmF3′H CmDFR appeared be responsible the ratio cyanidin pelargonidin derivatives miniata, low lack cyanidins Moreover, promoter could not activated by CmMYBAs, suggesting it controlled novel regulators. Only two CmTPSs functional, with CmTPS2 synthesis, contributing monotonous flower terpenes miniata. CmCCD1a CmCCD1b able cleave carotenoids at 5,6 (5′,6′), 9,10 (9′,10′) positions generate apocarotenoids, substrates found low-quantities specific subcellular localizations CmCCD1s constrain apocarotenoid release. Consequently, activating F3′H introducing F3′5′H versatile TPS may effective ways modify scent, respectively. Alternatively, modifying flux CCD1 localization affect simultaneously. Taking these together, present study provides preliminary deciphering genetic constraints underlying development, proposes possible schemes further modification ornamental traits other plants.

Язык: Английский

---