Agriculture,
Год журнала:
2025,
Номер
15(9), С. 950 - 950
Опубликована: Апрель 27, 2025
Chrysanthemum
morifolium,
a
major
cut
flower
worldwide,
undergoes
petal
fading
under
heat
stress
due
to
reduced
anthocyanin
accumulation,
significantly
compromising
its
ornamental
value.
While
previous
studies
have
focused
on
heat-induced
inhibition
of
biosynthesis,
the
mechanisms
governing
degradation
remain
unclear.
In
this
study,
‘Nannong
Fencui’
chrysanthemums
at
full
bloom—when
accumulation
peaks—were
exposed
35
°C,
while
control
group
was
maintained
22
assess
effects
metabolism,
including
both
biosynthesis
and
degradation.
Transcriptomic
analysis
identified
nine
core
structural
genes
three
key
transcription
factors
involved
in
along
with
twelve
linked
enzymatic
Notably,
FPKM
values
for
were
extremely
low
groups,
indicating
that
largely
inactive
bloom.
Untargeted
metabolomic
30
most
enriched
metabolic
pathways.
Compared
control,
treatment
led
significant
increase
93
metabolites
(FC
>
1.5,
p
<
0.05,
VIP
1)
decrease
160
1/1.5,
1).
Cyanidin
glucoside,
primary
chrysanthemum
petals,
decreased
treatment,
potential
product,
protocatechuic
acid,
undetectable.
Meanwhile,
5-carboxyvanillic
acid
levels
increased
heat-treated
suggesting
may
been
converted
into
via
an
O-methylation
pathway.
These
findings
provide
new
insights
regulation
anthocyanins
offer
strategies
maintaining
color
quality
during
summer
production,
highlighting
candidate
(CmPRXs
CmOMT1)
future
functional
validation
breeding
efforts
aimed
improving
tolerance
stability.
The Plant Journal,
Год журнала:
2025,
Номер
121(6)
Опубликована: Март 1, 2025
SUMMARY
Catechins
were
diversely
accumulated
in
Thea
plants
and
crucial
for
tea
flavor,
yet
the
mechanism
underlying
diverse
catechins
distribution
remained
elusive.
We
herein
collected
a
total
of
19
12
non‐
to
investigate
their
mechanism.
Results
showed
that
pattern
cultivated
significantly
differs
from
wild
relatives.
(+)‐Gallocatechin
gallate
(GCG)
was
detected
over
50%
but
almost
undetectable
plants.
Conversely,
(−)‐Epigallocatechin
(EGCG)
extensively
distributed
cultivars
extremely
low
few
relatives
such
as
Camellia
tetracocca
C.
ptilophylla
.
Expression
analysis
found
expression
flavonoid
3′,5′‐hydroxylase
(
F3′5′H
)
highly
correlated
with
EGCG
accumulation
Yeast
one‐hybrid
luciferase
assays
CsMYB1,
key
regulator,
could
bind
promoter
activate
its
promote
plants;
it
unable
due
14‐bp
deletion
promoter,
leading
content
EGCG.
also
silencing
anthocyanidin
synthase
ANS
enhanced
metabolic
flux
toward
GCG
not
plants,
consistent
observation
high
expression.
Overall,
results
illustrated
variation
would
help
facilitate
utilization
future
breeding.
Plant Cell & Environment,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 23, 2025
ABSTRACT
High
temperatures
significantly
affect
tea
yield
and
quality.
Arginine
methylation
is
crucial
for
plant
growth
environmental
adaptation.
However,
its
role
in
regulating
responses
to
high
remains
unclear.
In
this
study,
we
identified
an
important
Type
II
arginine
methyltransferase,
PRMT5,
plants
confirmed
methyltransferase
activity
both
vivo
vitro.
Our
findings
revealed
that
CsPRMT5‐mediated
symmetric
dimethylation
of
histone
H4R3
(H4R3sme2)
was
markedly
reduced
under
high‐temperature
conditions
plants.
Both
the
inhibitor
gene‐silencing
approaches
led
decreased
levels
H4R3sme2
modification,
resulting
alterations
theanine
catechins.
We
employed
a
genome‐wide
approach
analyze
RNA
sequencing
(RNA‐seq)
subjected
ambient
temperatures,
PRMT5
inhibitors,
silencing,
along
with
CsPRMT5
chromatin
immunoprecipitation
(ChIP‐seq).
Comparative
analysis
these
datasets
indicated
genes
regulated
by
were
predominantly
enriched
within
reactive
oxygen
species
(ROS),
calcium
ion,
hormone
signalling
pathways
elevated
temperature
conditions.
Furthermore,
validated
CsCDPK9
as
target
gene
found
silencing
resulted
increased
content
catechin
at
temperatures.
suggest
plays
pivotal
plants,
well
their
adaptability
fluctuations
This
study
provides
new
insights
into
breeding
strategies
aimed
developing
crops
are
better
equipped
withstand
changes
induced
climate
change.
Agriculture,
Год журнала:
2025,
Номер
15(9), С. 950 - 950
Опубликована: Апрель 27, 2025
Chrysanthemum
morifolium,
a
major
cut
flower
worldwide,
undergoes
petal
fading
under
heat
stress
due
to
reduced
anthocyanin
accumulation,
significantly
compromising
its
ornamental
value.
While
previous
studies
have
focused
on
heat-induced
inhibition
of
biosynthesis,
the
mechanisms
governing
degradation
remain
unclear.
In
this
study,
‘Nannong
Fencui’
chrysanthemums
at
full
bloom—when
accumulation
peaks—were
exposed
35
°C,
while
control
group
was
maintained
22
assess
effects
metabolism,
including
both
biosynthesis
and
degradation.
Transcriptomic
analysis
identified
nine
core
structural
genes
three
key
transcription
factors
involved
in
along
with
twelve
linked
enzymatic
Notably,
FPKM
values
for
were
extremely
low
groups,
indicating
that
largely
inactive
bloom.
Untargeted
metabolomic
30
most
enriched
metabolic
pathways.
Compared
control,
treatment
led
significant
increase
93
metabolites
(FC
>
1.5,
p
<
0.05,
VIP
1)
decrease
160
1/1.5,
1).
Cyanidin
glucoside,
primary
chrysanthemum
petals,
decreased
treatment,
potential
product,
protocatechuic
acid,
undetectable.
Meanwhile,
5-carboxyvanillic
acid
levels
increased
heat-treated
suggesting
may
been
converted
into
via
an
O-methylation
pathway.
These
findings
provide
new
insights
regulation
anthocyanins
offer
strategies
maintaining
color
quality
during
summer
production,
highlighting
candidate
(CmPRXs
CmOMT1)
future
functional
validation
breeding
efforts
aimed
improving
tolerance
stability.
