Plant and algal lipidomes: Analysis, composition, and their societal significance
Progress in Lipid Research,
Год журнала:
2024,
Номер
96, С. 101290 - 101290
Опубликована: Июль 31, 2024
niveau
recherche,
publiés
ou
non,
émanant
des
établissements
d'enseignement
et
de
recherche
français
étrangers,
laboratoires
publics
privés.
Exploring lipid–protein interactions in plant membranes
Journal of Experimental Botany,
Год журнала:
2024,
Номер
75(17), С. 5251 - 5266
Опубликована: Май 6, 2024
Abstract
Once
regarded
as
mere
membrane
building
blocks,
lipids
are
now
recognized
diverse
and
intricate
players
that
mold
the
functions,
identities,
responses
of
cellular
membranes.
Although
interactions
with
integral
peripheral
proteins
crucial
for
their
localization,
activity,
function,
how
bind
is
still
far
from
being
thoroughly
explored.
Describing
characterizing
these
dynamic
protein–lipid
thus
essential
to
understanding
membrane-associated
processes.
Here
we
review
current
range
experimental
techniques
employed
study
plant
interactions,
integrating
various
methods.
We
summarize
principles,
advantages,
limitations
classical
in
vitro
biochemical
approaches,
including
overlays
liposome
binding
assays,
complement
them
vivo
microscopic
centered
around
use
genetically
encoded
lipid
sensors
pharmacological
or
genetic
manipulation
tools.
also
highlight
several
emerging
awaiting
advancement
into
research
emphasize
need
complementary
strategies
key
elucidating
mechanistic
roles
cell
biology.
Язык: Английский
Sphingolipid long chain bases as mediators of cell death in olive fruit abscission
Physiologia Plantarum,
Год журнала:
2025,
Номер
177(1)
Опубликована: Янв. 1, 2025
Abstract
Plant
sphingolipids
are
lipophilic
membrane
components
essential
for
different
cellular
functions
but
they
also
act
as
signaling
molecules
in
various
aspects
of
plant
development.
However,
the
interaction
between
and
abscission
remains
largely
uncharacterized.
Here,
possible
role
regulating
fruit
was
examined
zone
(AZ)
olive
fruit.
To
this
end,
sphingolipid
levels
were
manipulated
through
application
exogenous
long‐chain
bases
(LCBs)
or
biosynthesis
inhibitors,
their
effects
on
well
LCB/gene
expression,
hormones,
reactive
oxygen
species
(ROS)
cell
death
AZ
Our
data
indicated
that
sphinganine
(d18:0)
induced
abscission,
whereas
sphingosine
(d18:1)
phytosphingosine
(t18:0)
phosphorylated
derivatives
did
not
have
an
effect
abscission.
Moreover,
inhibition
LCB
kinase
ceramide
synthase,
which
increases
AZ,
reduced
break
strength.
This
induction
is
associated
with
elevated
ROS
enriched
salicylic
acid
(SA)
jasmonic
(JA).
Along
same
line,
programmed
(PCD)
particularly
evident
distal
side
AZ.
These
suggest
endogenous
d18:0
plays
a
key
molecule
functioning
upstream
SA/JA
pathway
mediating
PCD
spatially
regulated
during
Overall,
findings
reported
here
provide
insight
into
complex
connection
LCBs,
uncovering
process.
Язык: Английский
Effects of phospholipase C inhibition on the regulation of membrane lipid metabolism in maize leaves
Frontiers in Plant Science,
Год журнала:
2025,
Номер
16
Опубликована: Апрель 1, 2025
Phospholipase
C
(PLC)
is
an
enzyme
that
catalyzes
the
hydrolysis
of
glycerophospholipids
and
can
be
classified
as
phospholipase-specific
PLC
(PI-PLC)
non-specific
(NPC)
depending
on
its
specific
substrate.
In
this
study,
neomycin
sulfate
(NS,
100
mM)
was
used
to
inhibit
activity
phospholipase
in
maize
seedlings,
effect
lipid
metabolism
investigated
by
combined
analysis
transcriptome
lipidome.
Lipidomic
showed
when
inhibited,
content
phospholipids
more
than
10%
increase
due
elevated
accumulation
PC
PE.
At
same
time,
transcriptomic
data
suggested
activation
de
novo
biosynthesis
PE
from
choline
ethanolamine
via
upregulated
CCT
PECT,
respectively.
Thus,
inhibition
phospholipid
enhancement
synthesis
together
contribute
total
phospholipids.
Glycolipids
account
for
around
60%
lipids
leaves.
Under
NS
treatment,
MGDG
decreased
significantly,
while
DGDG
increased.
Our
results
indicate
treatment
may
cause
damage
photosynthetic
membrane
lipids,
whereas
bilayer
provide
a
kind
protection
maintain
stability
chloroplasts.
These
findings
suggest
plays
key
role
plasma
metabolism.
Язык: Английский
A New Approach to Detect and Semi-quantify All Molecular Species and Classes of Anionic Phospholipids Simultaneously in Plant Samples
BIO-PROTOCOL,
Год журнала:
2025,
Номер
15(1369)
Опубликована: Янв. 1, 2025
Membranes
are
very
complex
and
dynamic
structures
that
essential
for
plant
cellular
functions
whose
lipidic
composition
can
be
influenced
by
numerous
factors.
Anionic
phospholipids,
which
include
phosphatidylserine,
phosphatidic
acid,
phosphatidylinositol,
phosphoinositides
key
components
of
these
membranes
as
they
involved
in
cell
signaling
even
slight
modifications
their
quantities
may
largely
impact
the
metabolism.
However,
presence
compounds
low
amounts,
well
poor
stability
during
analysis
mass
spectrometry,
make
study
complicated.
In
addition,
precise
quantification
all
anionic
phospholipid
species
is
not
possible
lipid
separation
using
thin-layer
chromatography
followed
fatty
acyl
chains
gas
chromatography.
Here,
we
describe
a
straightforward
strategy
extraction
semi-quantification
from
samples.
Our
method
based
on
derivatization
more
especially
methylation
trimethylsilyldiazomethane,
high-performance
liquid
coupled
with
triple
quadrupole
spectrometer.
This
approach
allows
improving
sensitivity
phospholipids
samples,
will
help
to
gain
deeper
insights
into
dynamics
parts
signaling.
Key
features
•
Extraction,
derivatization,
(PA,
PS,
PI,
PIPs)
The
trimethylsilyldiazomethane
improves
therefore
analysis.
HPLC-QQQ-MS/MS
methylated
high
sensitivity.
Allows
investigate
diversity
anionics
wide
range
samples
(leaves,
cultivated
cells,
membranes,
…).
Язык: Английский
Identification of INOSITOL PHOSPHORYLCERAMIDE SYNTHASE 2 (IPCS2) as a new rate‐limiting component in Arabidopsis pathogen entry control
The Plant Journal,
Год журнала:
2025,
Номер
122(2)
Опубликована: Апрель 1, 2025
SUMMARY
INOSITOL
PHOSPHORYLCERAMIDE
SYNTHASE
2
(IPCS2)
is
involved
in
the
biosynthesis
of
complex
sphingolipids
at
trans
‐Golgi
network
(TGN).
Here,
we
demonstrate
a
role
IPCS2
penetration
resistance
against
non‐adapted
powdery
mildew
fungi.
A
novel
ipcs2
W205
*
mutant
was
recovered
from
forward
genetic
screen
for
Arabidopsis
plants
with
enhanced
epidermal
cell
entry
success
barley
fungus
Blumeria
graminis
f.
sp.
hordei
(
Bgh
).
yeast
complementation
assay
and
sphingolipidomic
approach
revealed
that
represents
knock‐out
lacks
IPCS2‐specific
enzymatic
activity.
Further
analyses
suggested
IPCS2‐derived
glycosyl
inositol
phosphorylceramides
(GIPCs)
are
required
control
fungal
intruders.
Confocal
laser
scanning
microscopy
(CLSM)
studies
indicated
upon
pathogen
attack,
remains
TGN
to
produce
GIPCs,
while
focal
accumulation
defense
cargo
PENETRATION
3
(PEN3)
sites
reduced
background.
Thus,
propose
model
which
sorting
events
facilitated
by
sphingolipids,
regulating
polar
secretion
PEN3
host‐pathogen
contact
terminate
ingress.
Язык: Английский
Sterols in plant biology – Advances in studying membrane dynamics
The Cell Surface,
Год журнала:
2025,
Номер
unknown, С. 100147 - 100147
Опубликована: Май 1, 2025
Язык: Английский
Membrane nanodomains to shape plant cellular functions and signaling
New Phytologist,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 25, 2024
Summary
Plasma
membrane
(PM)
nanodomains
have
emerged
as
pivotal
elements
in
the
regulation
of
plant
cellular
functions
and
signal
transduction.
These
nanoscale
regions,
enriched
specific
lipids
proteins,
behave
regulatory/signaling
hubs
spatially
temporally
coordinating
critical
functions.
In
this
review,
we
first
examine
mechanisms
underlying
formation
maintenance
PM
cells,
highlighting
roles
lipid
composition,
protein
oligomerization
interactions
with
cytoskeletal
cell
wall
components.
Then,
discuss
how
act
organizing
centers
by
mediating
protein–protein
that
orchestrate
essential
processes
such
symbiosis,
defense
against
pathogens,
ion
transport
or
hormonal
reactive
oxygen
species
(ROS)
signaling.
Finally,
introduce
concept
nanoenvironments,
where
localized
physicochemical
variations
are
generated
very
close
proximity
nanodomains,
response
to
stimuli.
After
decoding
a
dedicated
machinery
likely
vicinity
enrichment
secondary
messengers,
ROS
Ca
2+
,
would
allow
downstream
responses.
This
review
provides
insights
into
dynamic
nature
proposes
future
research
better
understand
their
contribution
intricate
signaling
networks
govern
development
stress
Язык: Английский