International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 143062 - 143062
Опубликована: Апрель 1, 2025
Язык: Английский
International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 143062 - 143062
Опубликована: Апрель 1, 2025
Язык: Английский
Scientia Horticulturae, Год журнала: 2025, Номер 341, С. 113958 - 113958
Опубликована: Янв. 16, 2025
Язык: Английский
Процитировано
2Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown
Опубликована: Март 19, 2025
Flavonoid rutinosides, a group of bioactive compounds in Anoectochilus roxburghii, contribute greatly to the plant's beneficial effects on human health. However, glycosylation mechanism flavonoid rutinosides A. roxburghii remains unclear. In this study, two efficient and selective glycosyltransferases, AUTG25 AUTG23, involved biosynthesis narcissoside, major rutinoside were identified through transcriptome analysis functional validation. could regioselectively catalyze 3-O-glucosylation isorhamnetin produce 3-O-glucoside, while AUTG23 further 6"-O-rhamnosylation generate narcissoside. Both exhibited high positional sugar donor selectivities catalytic reaction. Homology modeling site-directed mutagenesis showed that H20, E83, E385, F143 E280, E89, D188, W327, D369, Y191 may be critical for their functions. Transient expression Nicotiana benthamiana finally confirmed possesses flavonol-3-O-glucosyltransferase activity has flavonol-3-O-glucoside (1→6) rhamnosyltransferase activity. This study clarified provided candidate UDP-dependent glycosyltransferase genes narcissoside roxburghii.
Язык: Английский
Процитировано
0International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 143062 - 143062
Опубликована: Апрель 1, 2025
Язык: Английский
Процитировано
0