Transcriptomic and metabolomic analyses reveal the mechanism of cold chain breaks accelerate postharvest kiwifruit ripening and flavonoid loss

Scientia Horticulturae, Journal Year: 2025, Volume and Issue: 341, P. 113958 - 113958

Published: Jan. 16, 2025

Language: Английский

Identification and Functional Characterization of Two UDP-Glycosyltransferases Involved in Narcissoside Biosynthesis in Anoectochilus roxburghii

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 19, 2025

Flavonoid rutinosides, a group of bioactive compounds in Anoectochilus roxburghii, contribute greatly to the plant's beneficial effects on human health. However, glycosylation mechanism flavonoid rutinosides A. roxburghii remains unclear. In this study, two efficient and selective glycosyltransferases, AUTG25 AUTG23, involved biosynthesis narcissoside, major rutinoside were identified through transcriptome analysis functional validation. could regioselectively catalyze 3-O-glucosylation isorhamnetin produce 3-O-glucoside, while AUTG23 further 6"-O-rhamnosylation generate narcissoside. Both exhibited high positional sugar donor selectivities catalytic reaction. Homology modeling site-directed mutagenesis showed that H20, E83, E385, F143 E280, E89, D188, W327, D369, Y191 may be critical for their functions. Transient expression Nicotiana benthamiana finally confirmed possesses flavonol-3-O-glucosyltransferase activity has flavonol-3-O-glucoside (1→6) rhamnosyltransferase activity. This study clarified provided candidate UDP-dependent glycosyltransferase genes narcissoside roxburghii.

Language: Английский

Identification and characteristic analysis of PavUGT48 as a novel UDP-glycosyltransferase with dual functions on anthocyanin and amygdalin biosynthesis in sweet cherry

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 143062 - 143062

Published: April 1, 2025

Language: Английский