Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Март 11, 2025

Background Triterpenoid compounds such as betulinic acid (BA) and oleanolic (OA) exhibit considerable pharmacological activities. However, their current production primarily relies on plant extraction chemical synthesis, methods that are often plagued by low efficiency, complex processes, environmental concerns. Microbial-based synthesis has emerged one of the most effective approaches for producing BA OA. Results This study presented first identification filamentous fungal strains efficiently synthesizing both The strain ZBS49 is a newly identified species Clonostachys isolated from Gleditsia japonica Miq. (showing 99.82% sequence identity), produced 47.7 mg/L BA. XJ1-1, characterized Colletotrichum gloeosporioides Cannabis sativa L., yielded 65.76 After optimizing culture medium cultivation conditions, yields XJ1-1 increased to 288.97 86.14 mg/L, representing improvements 506% 31%, respectively. Furthermore, we discovered extract significantly inhibited hepatocellular cancer cells (SMMC-7721 HepG2) in dose-dependent manner, with minimum inhibitory concentration 70 µM. Genomic analysis sp. elucidated presence 16 putative genes was related triterpenoid biosynthesis 6 distinct terpene biosynthetic gene clusters. Among 145 CYP450, 5 involved C-28 oxidation were predicted. Conclusions research underscores effectiveness fungi biotechnological platform efficient its derivatives, highlighting potential applications therapy. these findings provide valuable genetic resources establish robust technical theoretical framework utilizing microbial triterpenoids.

Язык: Английский