A new Clonostachys sp. ZBS49 filamentous fungus with high production of betulinic acid and its inhibitory effect on liver cancer cells

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

Background Triterpenoid compounds such as betulinic acid (BA) and oleanolic (OA) exhibit considerable pharmacological activities. However, their current production primarily relies on plant extraction chemical synthesis, methods that are often plagued by low efficiency, complex processes, environmental concerns. Microbial-based synthesis has emerged one of the most effective approaches for producing BA OA. Results This study presented first identification filamentous fungal strains efficiently synthesizing both The strain ZBS49 is a newly identified species Clonostachys isolated from Gleditsia japonica Miq. (showing 99.82% sequence identity), produced 47.7 mg/L BA. XJ1-1, characterized Colletotrichum gloeosporioides Cannabis sativa L., yielded 65.76 After optimizing culture medium cultivation conditions, yields XJ1-1 increased to 288.97 86.14 mg/L, representing improvements 506% 31%, respectively. Furthermore, we discovered extract significantly inhibited hepatocellular cancer cells (SMMC-7721 HepG2) in dose-dependent manner, with minimum inhibitory concentration 70 µM. Genomic analysis sp. elucidated presence 16 putative genes was related triterpenoid biosynthesis 6 distinct terpene biosynthetic gene clusters. Among 145 CYP450, 5 involved C-28 oxidation were predicted. Conclusions research underscores effectiveness fungi biotechnological platform efficient its derivatives, highlighting potential applications therapy. these findings provide valuable genetic resources establish robust technical theoretical framework utilizing microbial triterpenoids.

Language: Английский

Research progress in the extraction, purification, structural features, biological activities, and structure-activity relationships from Prunella vulgaris polysaccharides

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 307, P. 141957 - 141957

Published: March 10, 2025

Language: Английский